The Experts below are selected from a list of 1269 Experts worldwide ranked by ideXlab platform
Tetsuro Oshika - One of the best experts on this subject based on the ideXlab platform.
-
comparison of triamcinolone acetonide 11 deoxycortisol and other lipid formulae for the visualization of vitreous body in the anterior chamber after posterior capsule rupture in animal models
Acta Ophthalmologica, 2008Co-Authors: Yuichi Kaji, Takahiro Hiraoka, Fumiki Okamoto, Hiroki Asano, Tetsuro OshikaAbstract:. Purpose: The efficacy and toxicity of triamcinolone acetonide and other lipid formulae – calcium palmitate, cholesterol and 11-Deoxycortisol –in the visualization of the prolapsed vitreous body in the anterior chamber after posterior capsule rupture were investigated in animal models. Methods: In porcine eyes, a suspension of calcium palmitate, cholesterol, triamcinolone acetonide and 11-Deoxycortisol was injected into the anterior chamber after intentionally creating posterior capsule rupture. Following gentle irrigation and aspiration, the vitreous body prolapsed in the anterior chamber was removed using an anterior vitrectomy cutter. In phakic rabbit eyes, the side-effects of the reagents were assessed for biomicroscopic appearance, intraocular pressure (IOP) and corneal histology. Results: The suspension of calcium palmitate, cholesterol, triamcinolone acetonide and 11-Deoxycortisol was effective in the visualization of the vitreous body prolapsed in the anterior chamber after posterior capsule rupture. When cholesterol and calcium palmitate were injected into the anterior chamber, they remained there; this induced a significant increase in IOP and corneal oedema. In contrast, most of the triamcinolone acetonide and 11-Deoxycortisol that was injected into the anterior chamber had disappeared a day after the injection without affecting IOP or corneal endothelial density. When injected into the intravitreous cavity, triamcinolone led to a significant increase in IOP 2 and 4 weeks after the injection. However, calcium palmitate, cholesterol and 11-Deoxycortisol injected into the vitreous cavity had no effect on IOP at 4 weeks. Conclusion: The suspension of triamcinolone acetonide and 11-Deoxycortisol was effective in visualizing the vitreous body prolapsed in the anterior chamber after posterior capsule rupture. However, the amount of the reagent must be kept to a minimum to prevent the potential risk of ocular toxicities and postoperative late-onset ocular hypertension.
-
clinical application of 11 deoxycortisol in visualizing prolapsed vitreous body after posterior capsule rupture in cataract surgery
Journal of Cataract and Refractive Surgery, 2005Co-Authors: Yuichi Kaji, Takahiro Hiraoka, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Purpose To compare the temperature profiles of 3 popular phacoemulsification units (Alcon AdvanTec Legacy, Bausch & Lomb Millennium, and AMO Sovereign WhiteStar) under similar operating conditions in air. Setting Jules Stein Eye Institute and the Department of Ophthalmology, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California, USA. Methods Phacoemulsification probes from the 3 units were placed side-by-side in air and imaged in the infrared using an FLIR model P60 ThermaCAM. The highest temperature produced by each probe was measured 10 and 30 seconds after power application. Testing was performed under conditions that might produce a corneal burn. Irrigation flow was set at 1 cc/min to simulate a tight incision. Aspiration flow was set at 0 cc/min to simulate occlusion. Wound compression was simulated by suspending 22.6-gram weights from the silicone sleeves. The Alcon AdvanTec Legacy and Bausch & Lomb Millennium were operated in pulse mode at 15 Hertz; 50% duty cycle; and 10%, 30% and 50% power. The AMO Sovereign WhiteStar was operated in both C/F (56 Hz, 33% duty cycle) and C/L (33 Hz, 20% duty cycle) modes. Results The Millennium and the Sovereign generated higher temperatures than the Legacy with almost all experimental conditions (at 10%, 30%, and 50% powers; with and without sleeve compression; with manufacturers' and identical irrigating sleeves; and in continuous and pulse modes). Conclusions The Bausch & Lomb Millennium and the AMO Sovereign WhiteStar, operating in both pulse and continuous modes, generated higher peak temperatures than the Alcon AdvanTec Legacy with very controlled operating conditions in air and with a variety of power, load, and duty-cycle settings.
-
visualizing the vitreous body in the anterior chamber using 11 deoxycortisol after posterior capsule rupture in an animal model
Ophthalmology, 2004Co-Authors: Yuichi Kaji, Beihua Hu, N Yamane, Takahiro Hiraoka, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Abstract Objective To develop a new technique to visualize vitreous body prolapsed in the anterior chamber using 11-Deoxycortisol. Study design Experimental study. Methods An animal model of posterior capsule rupture was developed to investigate the usefulness of 11-Deoxycortisol, a precursor of cortisol without steroid activity. After the intentional creation of posterior capsule rupture, the suspension of 11-Deoxycortisol was injected into the anterior chamber of rabbit eyes. After gentle irrigation and aspiration, the vitreous body that had prolapsed into the anterior chamber was removed using an anterior vitrectomy cutter. To investigate the safety of 11-Deoxycortisol, the biomicroscopic appearance, intraocular pressure (IOP), corneal endothelial count, and microstructure of the corneal endothelium were examined in the rabbits that received injections of 11-Deoxycortisol in the anterior chamber. Results In our posterior capsule rupture model, the vitreous in the anterior chamber became clearly visible, with 11-Deoxycortisol showing white particles entrapped on its surface. The injection of 11-Deoxycortisol facilitated the complete removal of the vitreous body from the anterior chamber. In intact rabbit eyes, most of the injected 11-Deoxycortisol had disappeared from the anterior chamber by 12 hours after injection. The injection of 11-Deoxycortisol had no effect on IOP, corneal endothelial density, or the microstructure of the corneal endothelium. Conclusions The injection of 11-Deoxycortisol in the anterior chamber is useful in visualizing the vitreous body and has no significant side effects. This technique might reduce the intraoperative and postoperative complications of anterior vitrectomy after posterior capsule rupture.
-
visualizing vitreous body in the anterior chamber using 11 deoxycortisol after posterior capsule rupture in an animal model
Investigative Ophthalmology & Visual Science, 2004Co-Authors: Yuko Ishii, Beihua Hu, N Yamane, Takahiro Hiraoka, Yuichi Kaji, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Objective: To develop a new technique to visualize vitreous body prolapsed in the anterior chamber using 11-Deoxycortisol. Study Design: Experimental study. Methods: An animal model of posterior capsule rupture was developed to investigate the usefulness of 11-Deoxycortisol, a precursor of cortisol without steroid activity. After the intentional creation of posterior capsule rupture, the suspension of 11-Deoxycortisol was injected into the anterior chamber of rabbit eyes. After gentle irrigation and aspiration, the vitreous body that had prolapsed into the anterior chamber was removed using an anterior vitrectomy cutter. To investigate the safety of 11-Deoxycortisol, the biomicroscopic appearance, intraocular pressure (IOP), corneal endothelial count, and microstructure of the corneal endothelium were examined in the rabbits that received injections of 11-Deoxycortisol in the anterior chamber. Results: In our posterior capsule rupture model, the vitreous in the anterior chamber became clearly visible, with 11-Deoxycortisol showing white particles entrapped on its surface. The injection of 11-Deoxycortisol facilitated the complete removal of the vitreous body from the anterior chamber. In intact rabbit eyes, most of the injected 11-Deoxycortisol had disappeared from the anterior chamber by 12 hours after injection. The injection of 11-Deoxycortisol had no effect on IOP, corneal endothelial density, or the microstructure of the corneal endothelium. Conclusions: The injection of 11-Deoxycortisol in the anterior chamber is useful in visualizing the vitreous body and has no significant side effects. This technique might reduce the intraoperative and postoperative complications of anterior vitrectomy after posterior capsule rupture. Ophthalmology 2004;111:1334–1339 © 2004 by the American Academy of Ophthalmology.
Yuichi Kaji - One of the best experts on this subject based on the ideXlab platform.
-
comparison of triamcinolone acetonide 11 deoxycortisol and other lipid formulae for the visualization of vitreous body in the anterior chamber after posterior capsule rupture in animal models
Acta Ophthalmologica, 2008Co-Authors: Yuichi Kaji, Takahiro Hiraoka, Fumiki Okamoto, Hiroki Asano, Tetsuro OshikaAbstract:. Purpose: The efficacy and toxicity of triamcinolone acetonide and other lipid formulae – calcium palmitate, cholesterol and 11-Deoxycortisol –in the visualization of the prolapsed vitreous body in the anterior chamber after posterior capsule rupture were investigated in animal models. Methods: In porcine eyes, a suspension of calcium palmitate, cholesterol, triamcinolone acetonide and 11-Deoxycortisol was injected into the anterior chamber after intentionally creating posterior capsule rupture. Following gentle irrigation and aspiration, the vitreous body prolapsed in the anterior chamber was removed using an anterior vitrectomy cutter. In phakic rabbit eyes, the side-effects of the reagents were assessed for biomicroscopic appearance, intraocular pressure (IOP) and corneal histology. Results: The suspension of calcium palmitate, cholesterol, triamcinolone acetonide and 11-Deoxycortisol was effective in the visualization of the vitreous body prolapsed in the anterior chamber after posterior capsule rupture. When cholesterol and calcium palmitate were injected into the anterior chamber, they remained there; this induced a significant increase in IOP and corneal oedema. In contrast, most of the triamcinolone acetonide and 11-Deoxycortisol that was injected into the anterior chamber had disappeared a day after the injection without affecting IOP or corneal endothelial density. When injected into the intravitreous cavity, triamcinolone led to a significant increase in IOP 2 and 4 weeks after the injection. However, calcium palmitate, cholesterol and 11-Deoxycortisol injected into the vitreous cavity had no effect on IOP at 4 weeks. Conclusion: The suspension of triamcinolone acetonide and 11-Deoxycortisol was effective in visualizing the vitreous body prolapsed in the anterior chamber after posterior capsule rupture. However, the amount of the reagent must be kept to a minimum to prevent the potential risk of ocular toxicities and postoperative late-onset ocular hypertension.
-
clinical application of 11 deoxycortisol in visualizing prolapsed vitreous body after posterior capsule rupture in cataract surgery
Journal of Cataract and Refractive Surgery, 2005Co-Authors: Yuichi Kaji, Takahiro Hiraoka, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Purpose To compare the temperature profiles of 3 popular phacoemulsification units (Alcon AdvanTec Legacy, Bausch & Lomb Millennium, and AMO Sovereign WhiteStar) under similar operating conditions in air. Setting Jules Stein Eye Institute and the Department of Ophthalmology, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California, USA. Methods Phacoemulsification probes from the 3 units were placed side-by-side in air and imaged in the infrared using an FLIR model P60 ThermaCAM. The highest temperature produced by each probe was measured 10 and 30 seconds after power application. Testing was performed under conditions that might produce a corneal burn. Irrigation flow was set at 1 cc/min to simulate a tight incision. Aspiration flow was set at 0 cc/min to simulate occlusion. Wound compression was simulated by suspending 22.6-gram weights from the silicone sleeves. The Alcon AdvanTec Legacy and Bausch & Lomb Millennium were operated in pulse mode at 15 Hertz; 50% duty cycle; and 10%, 30% and 50% power. The AMO Sovereign WhiteStar was operated in both C/F (56 Hz, 33% duty cycle) and C/L (33 Hz, 20% duty cycle) modes. Results The Millennium and the Sovereign generated higher temperatures than the Legacy with almost all experimental conditions (at 10%, 30%, and 50% powers; with and without sleeve compression; with manufacturers' and identical irrigating sleeves; and in continuous and pulse modes). Conclusions The Bausch & Lomb Millennium and the AMO Sovereign WhiteStar, operating in both pulse and continuous modes, generated higher peak temperatures than the Alcon AdvanTec Legacy with very controlled operating conditions in air and with a variety of power, load, and duty-cycle settings.
-
visualizing the vitreous body in the anterior chamber using 11 deoxycortisol after posterior capsule rupture in an animal model
Ophthalmology, 2004Co-Authors: Yuichi Kaji, Beihua Hu, N Yamane, Takahiro Hiraoka, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Abstract Objective To develop a new technique to visualize vitreous body prolapsed in the anterior chamber using 11-Deoxycortisol. Study design Experimental study. Methods An animal model of posterior capsule rupture was developed to investigate the usefulness of 11-Deoxycortisol, a precursor of cortisol without steroid activity. After the intentional creation of posterior capsule rupture, the suspension of 11-Deoxycortisol was injected into the anterior chamber of rabbit eyes. After gentle irrigation and aspiration, the vitreous body that had prolapsed into the anterior chamber was removed using an anterior vitrectomy cutter. To investigate the safety of 11-Deoxycortisol, the biomicroscopic appearance, intraocular pressure (IOP), corneal endothelial count, and microstructure of the corneal endothelium were examined in the rabbits that received injections of 11-Deoxycortisol in the anterior chamber. Results In our posterior capsule rupture model, the vitreous in the anterior chamber became clearly visible, with 11-Deoxycortisol showing white particles entrapped on its surface. The injection of 11-Deoxycortisol facilitated the complete removal of the vitreous body from the anterior chamber. In intact rabbit eyes, most of the injected 11-Deoxycortisol had disappeared from the anterior chamber by 12 hours after injection. The injection of 11-Deoxycortisol had no effect on IOP, corneal endothelial density, or the microstructure of the corneal endothelium. Conclusions The injection of 11-Deoxycortisol in the anterior chamber is useful in visualizing the vitreous body and has no significant side effects. This technique might reduce the intraoperative and postoperative complications of anterior vitrectomy after posterior capsule rupture.
-
visualizing vitreous body in the anterior chamber using 11 deoxycortisol after posterior capsule rupture in an animal model
Investigative Ophthalmology & Visual Science, 2004Co-Authors: Yuko Ishii, Beihua Hu, N Yamane, Takahiro Hiraoka, Yuichi Kaji, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Objective: To develop a new technique to visualize vitreous body prolapsed in the anterior chamber using 11-Deoxycortisol. Study Design: Experimental study. Methods: An animal model of posterior capsule rupture was developed to investigate the usefulness of 11-Deoxycortisol, a precursor of cortisol without steroid activity. After the intentional creation of posterior capsule rupture, the suspension of 11-Deoxycortisol was injected into the anterior chamber of rabbit eyes. After gentle irrigation and aspiration, the vitreous body that had prolapsed into the anterior chamber was removed using an anterior vitrectomy cutter. To investigate the safety of 11-Deoxycortisol, the biomicroscopic appearance, intraocular pressure (IOP), corneal endothelial count, and microstructure of the corneal endothelium were examined in the rabbits that received injections of 11-Deoxycortisol in the anterior chamber. Results: In our posterior capsule rupture model, the vitreous in the anterior chamber became clearly visible, with 11-Deoxycortisol showing white particles entrapped on its surface. The injection of 11-Deoxycortisol facilitated the complete removal of the vitreous body from the anterior chamber. In intact rabbit eyes, most of the injected 11-Deoxycortisol had disappeared from the anterior chamber by 12 hours after injection. The injection of 11-Deoxycortisol had no effect on IOP, corneal endothelial density, or the microstructure of the corneal endothelium. Conclusions: The injection of 11-Deoxycortisol in the anterior chamber is useful in visualizing the vitreous body and has no significant side effects. This technique might reduce the intraoperative and postoperative complications of anterior vitrectomy after posterior capsule rupture. Ophthalmology 2004;111:1334–1339 © 2004 by the American Academy of Ophthalmology.
Stephen D Mccormick - One of the best experts on this subject based on the ideXlab platform.
-
11 deoxycortisol is a stress responsive and gluconeogenic hormone in a jawless vertebrate the sea lamprey petromyzon marinus
The Journal of Experimental Biology, 2021Co-Authors: Ciaran A Shaughnessy, Stephen D MccormickAbstract:Although corticosteroid-mediated hepatic gluconeogenic activity in response to stress has been extensively studied in fishes and other vertebrates, there is little information on the stress response in basal vertebrates. In sea lamprey (Petromyzon marinus), a representative member of the most basal extant vertebrate group Agnatha, 11-Deoxycortisol and deoxycorticosterone are the major circulating corticosteroids. The present study examined changes in circulating glucose and 11-Deoxycortisol concentrations in response to a physical stressor. Furthermore, the gluconeogenic actions of 11-Deoxycortisol and deoxycorticosterone were examined. Within 6 h after exposure of larval and juvenile sea lamprey to an acute handling stress, plasma 11-Deoxycortisol levels increased 15- and 6-fold, respectively, and plasma glucose increased 3- and 4-fold, respectively. Radiometric receptor binding studies revealed that a corticosteroid receptor (CR) is present in the liver at lower abundance than other tissues (gill and anterior intestine) and that the binding affinity of the liver CR was similar for 11-Deoxycortisol and deoxycorticosterone. Transcriptional tissue profiles indicate a wide distribution of cr transcription, kidney-specific transcription of steroidogenic acute regulatory protein (star), and liver-specific transcription of phosphoenolpyruvate carboxykinase (pepck). Ex vivo incubation of liver tissue with 11-Deoxycortisol resulted in dose-dependent increases in pepck mRNA levels. Finally, intraperitoneal administration of 11-Deoxycortisol and deoxycorticosterone demonstrated that only 11-Deoxycortisol resulted in an increase in plasma glucose. Together, these results provide the first direct evidence for the gluconeogenic activity of 11-Deoxycortisol in an Agnathan, indicating that corticosteroid regulation of plasma glucose is a basal trait among vertebrates.
-
11 deoxycortisol is a corticosteroid hormone in the lamprey
Proceedings of the National Academy of Sciences of the United States of America, 2010Co-Authors: David A Close, Stephen D Mccormick, Sangseon Yun, Andrew WildbillAbstract:Corticosteroid hormones are critical for controlling metabolism, hydromineral balance, and the stress response in vertebrates. Although corticosteroid hormones have been well characterized in most vertebrate groups, the identity of the earliest vertebrate corticosteroid hormone has remained elusive. Here we provide evidence that 11-Deoxycortisol is the corticosteroid hormone in the lamprey, a member of the agnathans that evolved more than 500 million years ago. We used RIA, HPLC, and mass spectrometry analysis to determine that 11-Deoxycortisol is the active corticosteroid present in lamprey plasma. We also characterized an 11-Deoxycortisol receptor extracted from sea lamprey gill cytosol. The receptor was highly specific for 11-Deoxycortisol and exhibited corticosteroid binding characteristics, including DNA binding. Furthermore, we observed that 11-Deoxycortisol was regulated by the hypothalamus–pituitary axis and responded to acute stress. 11-Deoxycortisol implants reduced sex steroid concentrations and up-regulated gill Na+, K+-ATPase, an enzyme critical for ion balance. We show here that 11-Deoxycortisol functioned as both a glucocorticoid and a mineralocorticoid in the lamprey. Our findings indicate that a complex and highly specific corticosteroid signaling pathway evolved at least 500 million years ago with the arrival of the earliest vertebrate.
Takahiro Hiraoka - One of the best experts on this subject based on the ideXlab platform.
-
comparison of triamcinolone acetonide 11 deoxycortisol and other lipid formulae for the visualization of vitreous body in the anterior chamber after posterior capsule rupture in animal models
Acta Ophthalmologica, 2008Co-Authors: Yuichi Kaji, Takahiro Hiraoka, Fumiki Okamoto, Hiroki Asano, Tetsuro OshikaAbstract:. Purpose: The efficacy and toxicity of triamcinolone acetonide and other lipid formulae – calcium palmitate, cholesterol and 11-Deoxycortisol –in the visualization of the prolapsed vitreous body in the anterior chamber after posterior capsule rupture were investigated in animal models. Methods: In porcine eyes, a suspension of calcium palmitate, cholesterol, triamcinolone acetonide and 11-Deoxycortisol was injected into the anterior chamber after intentionally creating posterior capsule rupture. Following gentle irrigation and aspiration, the vitreous body prolapsed in the anterior chamber was removed using an anterior vitrectomy cutter. In phakic rabbit eyes, the side-effects of the reagents were assessed for biomicroscopic appearance, intraocular pressure (IOP) and corneal histology. Results: The suspension of calcium palmitate, cholesterol, triamcinolone acetonide and 11-Deoxycortisol was effective in the visualization of the vitreous body prolapsed in the anterior chamber after posterior capsule rupture. When cholesterol and calcium palmitate were injected into the anterior chamber, they remained there; this induced a significant increase in IOP and corneal oedema. In contrast, most of the triamcinolone acetonide and 11-Deoxycortisol that was injected into the anterior chamber had disappeared a day after the injection without affecting IOP or corneal endothelial density. When injected into the intravitreous cavity, triamcinolone led to a significant increase in IOP 2 and 4 weeks after the injection. However, calcium palmitate, cholesterol and 11-Deoxycortisol injected into the vitreous cavity had no effect on IOP at 4 weeks. Conclusion: The suspension of triamcinolone acetonide and 11-Deoxycortisol was effective in visualizing the vitreous body prolapsed in the anterior chamber after posterior capsule rupture. However, the amount of the reagent must be kept to a minimum to prevent the potential risk of ocular toxicities and postoperative late-onset ocular hypertension.
-
clinical application of 11 deoxycortisol in visualizing prolapsed vitreous body after posterior capsule rupture in cataract surgery
Journal of Cataract and Refractive Surgery, 2005Co-Authors: Yuichi Kaji, Takahiro Hiraoka, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Purpose To compare the temperature profiles of 3 popular phacoemulsification units (Alcon AdvanTec Legacy, Bausch & Lomb Millennium, and AMO Sovereign WhiteStar) under similar operating conditions in air. Setting Jules Stein Eye Institute and the Department of Ophthalmology, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California, USA. Methods Phacoemulsification probes from the 3 units were placed side-by-side in air and imaged in the infrared using an FLIR model P60 ThermaCAM. The highest temperature produced by each probe was measured 10 and 30 seconds after power application. Testing was performed under conditions that might produce a corneal burn. Irrigation flow was set at 1 cc/min to simulate a tight incision. Aspiration flow was set at 0 cc/min to simulate occlusion. Wound compression was simulated by suspending 22.6-gram weights from the silicone sleeves. The Alcon AdvanTec Legacy and Bausch & Lomb Millennium were operated in pulse mode at 15 Hertz; 50% duty cycle; and 10%, 30% and 50% power. The AMO Sovereign WhiteStar was operated in both C/F (56 Hz, 33% duty cycle) and C/L (33 Hz, 20% duty cycle) modes. Results The Millennium and the Sovereign generated higher temperatures than the Legacy with almost all experimental conditions (at 10%, 30%, and 50% powers; with and without sleeve compression; with manufacturers' and identical irrigating sleeves; and in continuous and pulse modes). Conclusions The Bausch & Lomb Millennium and the AMO Sovereign WhiteStar, operating in both pulse and continuous modes, generated higher peak temperatures than the Alcon AdvanTec Legacy with very controlled operating conditions in air and with a variety of power, load, and duty-cycle settings.
-
visualizing the vitreous body in the anterior chamber using 11 deoxycortisol after posterior capsule rupture in an animal model
Ophthalmology, 2004Co-Authors: Yuichi Kaji, Beihua Hu, N Yamane, Takahiro Hiraoka, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Abstract Objective To develop a new technique to visualize vitreous body prolapsed in the anterior chamber using 11-Deoxycortisol. Study design Experimental study. Methods An animal model of posterior capsule rupture was developed to investigate the usefulness of 11-Deoxycortisol, a precursor of cortisol without steroid activity. After the intentional creation of posterior capsule rupture, the suspension of 11-Deoxycortisol was injected into the anterior chamber of rabbit eyes. After gentle irrigation and aspiration, the vitreous body that had prolapsed into the anterior chamber was removed using an anterior vitrectomy cutter. To investigate the safety of 11-Deoxycortisol, the biomicroscopic appearance, intraocular pressure (IOP), corneal endothelial count, and microstructure of the corneal endothelium were examined in the rabbits that received injections of 11-Deoxycortisol in the anterior chamber. Results In our posterior capsule rupture model, the vitreous in the anterior chamber became clearly visible, with 11-Deoxycortisol showing white particles entrapped on its surface. The injection of 11-Deoxycortisol facilitated the complete removal of the vitreous body from the anterior chamber. In intact rabbit eyes, most of the injected 11-Deoxycortisol had disappeared from the anterior chamber by 12 hours after injection. The injection of 11-Deoxycortisol had no effect on IOP, corneal endothelial density, or the microstructure of the corneal endothelium. Conclusions The injection of 11-Deoxycortisol in the anterior chamber is useful in visualizing the vitreous body and has no significant side effects. This technique might reduce the intraoperative and postoperative complications of anterior vitrectomy after posterior capsule rupture.
-
visualizing vitreous body in the anterior chamber using 11 deoxycortisol after posterior capsule rupture in an animal model
Investigative Ophthalmology & Visual Science, 2004Co-Authors: Yuko Ishii, Beihua Hu, N Yamane, Takahiro Hiraoka, Yuichi Kaji, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Objective: To develop a new technique to visualize vitreous body prolapsed in the anterior chamber using 11-Deoxycortisol. Study Design: Experimental study. Methods: An animal model of posterior capsule rupture was developed to investigate the usefulness of 11-Deoxycortisol, a precursor of cortisol without steroid activity. After the intentional creation of posterior capsule rupture, the suspension of 11-Deoxycortisol was injected into the anterior chamber of rabbit eyes. After gentle irrigation and aspiration, the vitreous body that had prolapsed into the anterior chamber was removed using an anterior vitrectomy cutter. To investigate the safety of 11-Deoxycortisol, the biomicroscopic appearance, intraocular pressure (IOP), corneal endothelial count, and microstructure of the corneal endothelium were examined in the rabbits that received injections of 11-Deoxycortisol in the anterior chamber. Results: In our posterior capsule rupture model, the vitreous in the anterior chamber became clearly visible, with 11-Deoxycortisol showing white particles entrapped on its surface. The injection of 11-Deoxycortisol facilitated the complete removal of the vitreous body from the anterior chamber. In intact rabbit eyes, most of the injected 11-Deoxycortisol had disappeared from the anterior chamber by 12 hours after injection. The injection of 11-Deoxycortisol had no effect on IOP, corneal endothelial density, or the microstructure of the corneal endothelium. Conclusions: The injection of 11-Deoxycortisol in the anterior chamber is useful in visualizing the vitreous body and has no significant side effects. This technique might reduce the intraoperative and postoperative complications of anterior vitrectomy after posterior capsule rupture. Ophthalmology 2004;111:1334–1339 © 2004 by the American Academy of Ophthalmology.
Fumiki Okamoto - One of the best experts on this subject based on the ideXlab platform.
-
comparison of triamcinolone acetonide 11 deoxycortisol and other lipid formulae for the visualization of vitreous body in the anterior chamber after posterior capsule rupture in animal models
Acta Ophthalmologica, 2008Co-Authors: Yuichi Kaji, Takahiro Hiraoka, Fumiki Okamoto, Hiroki Asano, Tetsuro OshikaAbstract:. Purpose: The efficacy and toxicity of triamcinolone acetonide and other lipid formulae – calcium palmitate, cholesterol and 11-Deoxycortisol –in the visualization of the prolapsed vitreous body in the anterior chamber after posterior capsule rupture were investigated in animal models. Methods: In porcine eyes, a suspension of calcium palmitate, cholesterol, triamcinolone acetonide and 11-Deoxycortisol was injected into the anterior chamber after intentionally creating posterior capsule rupture. Following gentle irrigation and aspiration, the vitreous body prolapsed in the anterior chamber was removed using an anterior vitrectomy cutter. In phakic rabbit eyes, the side-effects of the reagents were assessed for biomicroscopic appearance, intraocular pressure (IOP) and corneal histology. Results: The suspension of calcium palmitate, cholesterol, triamcinolone acetonide and 11-Deoxycortisol was effective in the visualization of the vitreous body prolapsed in the anterior chamber after posterior capsule rupture. When cholesterol and calcium palmitate were injected into the anterior chamber, they remained there; this induced a significant increase in IOP and corneal oedema. In contrast, most of the triamcinolone acetonide and 11-Deoxycortisol that was injected into the anterior chamber had disappeared a day after the injection without affecting IOP or corneal endothelial density. When injected into the intravitreous cavity, triamcinolone led to a significant increase in IOP 2 and 4 weeks after the injection. However, calcium palmitate, cholesterol and 11-Deoxycortisol injected into the vitreous cavity had no effect on IOP at 4 weeks. Conclusion: The suspension of triamcinolone acetonide and 11-Deoxycortisol was effective in visualizing the vitreous body prolapsed in the anterior chamber after posterior capsule rupture. However, the amount of the reagent must be kept to a minimum to prevent the potential risk of ocular toxicities and postoperative late-onset ocular hypertension.
-
clinical application of 11 deoxycortisol in visualizing prolapsed vitreous body after posterior capsule rupture in cataract surgery
Journal of Cataract and Refractive Surgery, 2005Co-Authors: Yuichi Kaji, Takahiro Hiraoka, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Purpose To compare the temperature profiles of 3 popular phacoemulsification units (Alcon AdvanTec Legacy, Bausch & Lomb Millennium, and AMO Sovereign WhiteStar) under similar operating conditions in air. Setting Jules Stein Eye Institute and the Department of Ophthalmology, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California, USA. Methods Phacoemulsification probes from the 3 units were placed side-by-side in air and imaged in the infrared using an FLIR model P60 ThermaCAM. The highest temperature produced by each probe was measured 10 and 30 seconds after power application. Testing was performed under conditions that might produce a corneal burn. Irrigation flow was set at 1 cc/min to simulate a tight incision. Aspiration flow was set at 0 cc/min to simulate occlusion. Wound compression was simulated by suspending 22.6-gram weights from the silicone sleeves. The Alcon AdvanTec Legacy and Bausch & Lomb Millennium were operated in pulse mode at 15 Hertz; 50% duty cycle; and 10%, 30% and 50% power. The AMO Sovereign WhiteStar was operated in both C/F (56 Hz, 33% duty cycle) and C/L (33 Hz, 20% duty cycle) modes. Results The Millennium and the Sovereign generated higher temperatures than the Legacy with almost all experimental conditions (at 10%, 30%, and 50% powers; with and without sleeve compression; with manufacturers' and identical irrigating sleeves; and in continuous and pulse modes). Conclusions The Bausch & Lomb Millennium and the AMO Sovereign WhiteStar, operating in both pulse and continuous modes, generated higher peak temperatures than the Alcon AdvanTec Legacy with very controlled operating conditions in air and with a variety of power, load, and duty-cycle settings.
-
visualizing the vitreous body in the anterior chamber using 11 deoxycortisol after posterior capsule rupture in an animal model
Ophthalmology, 2004Co-Authors: Yuichi Kaji, Beihua Hu, N Yamane, Takahiro Hiraoka, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Abstract Objective To develop a new technique to visualize vitreous body prolapsed in the anterior chamber using 11-Deoxycortisol. Study design Experimental study. Methods An animal model of posterior capsule rupture was developed to investigate the usefulness of 11-Deoxycortisol, a precursor of cortisol without steroid activity. After the intentional creation of posterior capsule rupture, the suspension of 11-Deoxycortisol was injected into the anterior chamber of rabbit eyes. After gentle irrigation and aspiration, the vitreous body that had prolapsed into the anterior chamber was removed using an anterior vitrectomy cutter. To investigate the safety of 11-Deoxycortisol, the biomicroscopic appearance, intraocular pressure (IOP), corneal endothelial count, and microstructure of the corneal endothelium were examined in the rabbits that received injections of 11-Deoxycortisol in the anterior chamber. Results In our posterior capsule rupture model, the vitreous in the anterior chamber became clearly visible, with 11-Deoxycortisol showing white particles entrapped on its surface. The injection of 11-Deoxycortisol facilitated the complete removal of the vitreous body from the anterior chamber. In intact rabbit eyes, most of the injected 11-Deoxycortisol had disappeared from the anterior chamber by 12 hours after injection. The injection of 11-Deoxycortisol had no effect on IOP, corneal endothelial density, or the microstructure of the corneal endothelium. Conclusions The injection of 11-Deoxycortisol in the anterior chamber is useful in visualizing the vitreous body and has no significant side effects. This technique might reduce the intraoperative and postoperative complications of anterior vitrectomy after posterior capsule rupture.
-
visualizing vitreous body in the anterior chamber using 11 deoxycortisol after posterior capsule rupture in an animal model
Investigative Ophthalmology & Visual Science, 2004Co-Authors: Yuko Ishii, Beihua Hu, N Yamane, Takahiro Hiraoka, Yuichi Kaji, Fumiki Okamoto, M. Sato, Tetsuro OshikaAbstract:Objective: To develop a new technique to visualize vitreous body prolapsed in the anterior chamber using 11-Deoxycortisol. Study Design: Experimental study. Methods: An animal model of posterior capsule rupture was developed to investigate the usefulness of 11-Deoxycortisol, a precursor of cortisol without steroid activity. After the intentional creation of posterior capsule rupture, the suspension of 11-Deoxycortisol was injected into the anterior chamber of rabbit eyes. After gentle irrigation and aspiration, the vitreous body that had prolapsed into the anterior chamber was removed using an anterior vitrectomy cutter. To investigate the safety of 11-Deoxycortisol, the biomicroscopic appearance, intraocular pressure (IOP), corneal endothelial count, and microstructure of the corneal endothelium were examined in the rabbits that received injections of 11-Deoxycortisol in the anterior chamber. Results: In our posterior capsule rupture model, the vitreous in the anterior chamber became clearly visible, with 11-Deoxycortisol showing white particles entrapped on its surface. The injection of 11-Deoxycortisol facilitated the complete removal of the vitreous body from the anterior chamber. In intact rabbit eyes, most of the injected 11-Deoxycortisol had disappeared from the anterior chamber by 12 hours after injection. The injection of 11-Deoxycortisol had no effect on IOP, corneal endothelial density, or the microstructure of the corneal endothelium. Conclusions: The injection of 11-Deoxycortisol in the anterior chamber is useful in visualizing the vitreous body and has no significant side effects. This technique might reduce the intraoperative and postoperative complications of anterior vitrectomy after posterior capsule rupture. Ophthalmology 2004;111:1334–1339 © 2004 by the American Academy of Ophthalmology.
