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1H NMR Spectroscopy

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Anna Midelfart – One of the best experts on this subject based on the ideXlab platform.

  • Effect of UV-A and UV-B irradiation on the metabolic profile of aqueous humor in rabbits analyzed by 1H NMR Spectroscopy.
    Investigative ophthalmology & visual science, 2005
    Co-Authors: May-britt Tessem, Tone Frost Bathen, Jitka Cejkova, Anna Midelfart


    Effect of UV-A and UV-B Irradiation on the Metabolic Profile of Aqueous Humor in Rabbits Analyzed by 1H NMR Spectroscopy

  • Detection of different metabolites in the rabbit lens by high resolution 1H NMR Spectroscopy
    Current eye research, 1996
    Co-Authors: Anna Midelfart, Anne Dybdahl, Ingrid S. Gribbestad


    Purpose. To investigate the metabolic profile of the rabbit lens using high resolution 1H NMR Spectroscopy including two-dimensional shift correlated (COSY) technique.Methods. Perchloric acid extracts of the rabbit lens were analysed with a Bruker AM-500 spectrometer and the metabolites were assigned in the spectra. Some of these were also quantified.Results. More than 20 metabolites were detected in the perchloric acid extract of a single lens, including amino acids, nucleotides and other related compounds. Of particular importance is the ability to detect and identify glutathione, myo-inositol, scyllo-inositol and taurine.Conclusions. The study demonstrated the potential of 1H NMR Spectroscopy for monitoring the metabolic profile of the lens in normal and pathologic conditions.

  • High-resolution 1H NMR Spectroscopy of aqueous humour from rabbits
    Graefes Archive for Clinical and Experimental Ophthalmology, 1994
    Co-Authors: Ingrid S. Gribbestad, Anna Midelfart


    ⊎ Background: Detection of different substances in aqueous humour is important for evaluation of the disorders affecting the eye. The purpose of the present study was to apply highresolution proton (1H) nuclear magnetic resonance (NMR) Spectroscopy for extensive characterisation of the metabolites in the aqueous humour from rabbits. ⊎ Methods: High-resolution 1H NMR Spectroscopy, including two-dimensional shift correlated (COSY) technique, was performed on aqueous humour from rabbits. ⊎ Results: More than 20 metabolites were simultaneously detected and identified in high-resolution 1H NMR spectra of aqueous humour from rabbits. Some of these were also quantified. ⊎ Conclusion: High-resolution 1H NMR Spectroscopy is a valuable method for simultaneous detection of many different metabolites in aqueous humour.

Yoshio Nosaka – One of the best experts on this subject based on the ideXlab platform.

  • Photocatalytic Reaction Sites at the TiO2 Surface as Studied by Solid-State 1H NMR Spectroscopy
    Langmuir, 2003
    Co-Authors: Atsuko Y. Nosaka, Toshimichi Fujiwara, Hiromasa Yagi, And Hideo Akutsu, Yoshio Nosaka


    The adsorption and photodecomposition of ethanol that was employed as a model substrate of organic pollutants were investigated in the presence of adsorbed water with TiO2 by solid-state 1H NMR Spectroscopy in order to obtain information about the actual photocatalytic reaction sites of organic pollutants on TiO2 photocatalysts. Consequently it was revealed that ethanol molecules in practical conditions as contaminants were firmly captured in mobile physisorbed water layers to be photodecomposed to CO2 and water after photoillumination, suggesting that actual photocatalytic reactions should proceed in physisorbed water layers.

  • Photoinduced Changes of Adsorbed Water on a TiO2 Photocatalytic Film As Studied by 1H NMR Spectroscopy
    The Journal of Physical Chemistry B, 2003
    Co-Authors: Atsuko Y. Nosaka, Toshimichi Fujiwara, Hiromasa Yagi, And Hideo Akutsu, Eiichi Kojima, Yoshio Nosaka


    The enhancement of the signal intensity of water molecularly adsorbed on TiO2 films could be observed on UV irradiation by 1H NMR Spectroscopy, which has been difficult to elucidate so far with alternative spectroscopic techniques such as X-ray photoelectron Spectroscopy (XPS), temperature programmed desorption (TPD), and infrared reflection absorption Spectroscopy (IRAS). It was also confirmed that the enhanced NMR signals resulted from the photoinduced hydrophilic conversion of the film surface decreased back to the original state with the recovery to the less hydrophilic state of the TiO2 film surface when the film was kept in the dark.

Julian L. Griffin – One of the best experts on this subject based on the ideXlab platform.

  • High-Resolution Magic Angle Spinning 1H NMR Spectroscopy and Reverse Transcription-PCR Analysis of Apoptosis in a Rat Glioma
    Analytical chemistry, 2006
    Co-Authors: Julian L. Griffin, Cherie Blenkiron, Piia Valonen, Carlos Caldas, Risto A. Kauppinen


    The functional genomic approaches of transcriptomics, proteomics and metabolomics aim to measure the mRNA, protein or metabolite complement of a cell, tissue or organism. In this study we have investigated the compatibility of transcriptional analysis, using Reverse Transcription (RT)-PCR, and metabolite analysis, by high-resolution magic angle spinning (HRMAS) 1H NMR Spectroscopy, in BT4C rat glioma following the induction of programmed cell death. The metabolite and transcriptional changes that accompanied apoptosis were examined at 0, 4 and 8 days of ganciclovir/thymidine kinase gene therapy. Despite the high spinning speeds employed during HRMAS 1H NMR Spectroscopy of one-half of the tumor samples, RT-PCR analysis of the pro-apoptotic transcripts Bcl-2, BAK-1, caspase-9 and FAS was possible, producing similar results to those detected in the unspun half of the tumors. Furthermore, the expression of FAS was inversely correlated with some of the key metabolic changes across the time period examined incl…

  • High‐resolution diffusion and relaxation‐edited magic angle spinning 1H NMR Spectroscopy of intact liver tissue
    Magnetic resonance in medicine, 2003
    Co-Authors: O.m. Rooney, Jeremy K. Nicholson, J. Troke, Julian L. Griffin


    High-resolution magic angle spinning (HRMAS) 1H NMR Spectroscopy is ideal for monitoring the metabolic environment within tissues, particularly when spectra are weighted by physical properties such as T1 and T2 relaxation times and apparent diffusion coefficients (ADCs). In this study, spectral-editing using T1 and T2 relaxation times and ADCs at variable diffusion times was used in conjunction with HRMAS 1H NMR Spectroscopy at 14.1 T in liver tissue. To enhance the sensitivity of ADC measurements to low molecular weight metabolites a T2 spin echo was included in a standard stimulated gradient spin-echo sequence. Fatty liver induced in rats by chronic orotic acid feeding was investigated using this modified sequence. An increase in the combined ADC for the co-resonant peaks glucose, betaine, and TMAO during fatty liver disease was detected (ADCs = 0.60 ± 0.11 and 0.35 ± 0.1 * 10−9 m2s−1 (n = 3) for rats fed with and without orotic acid), indicative of a reduction in glucose and betaine and an increase in TMAO. Magn Reson Med 50:925–930, 2003. © 2003 Wiley-Liss, Inc.