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3 Flanking Region

The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform

Ikuko Mizuta – 1st expert on this subject based on the ideXlab platform

  • yy1 binds to α synuclein 3 Flanking Region snp and stimulates antisense noncoding rna expression
    Journal of Human Genetics, 2013
    Co-Authors: Ikuko Mizuta, Kazuaki Takafuji, Yuko Ando, Wataru Satake, Motoi Kanagawa, Kazuhiro Kobayashi, Shushi Nagamori, Takayuki Shinohara, Mitsutoshi Yamamoto

    Abstract:

    YY1 binds to α-synuclein 3′-Flanking Region SNP and stimulates antisense noncoding RNA expression

  • YY1 binds to α-synuclein 3′-Flanking Region SNP and stimulates antisense noncoding RNA expression
    Journal of Human Genetics, 2013
    Co-Authors: Ikuko Mizuta, Mitsutoshi Yamamoto, Kazuaki Takafuji, Yuko Ando, Wataru Satake, Motoi Kanagawa, Kazuhiro Kobayashi, Shushi Nagamori, Takayuki Shinohara, Nobutaka Hattori

    Abstract:

    α-synuclein ( SNCA ) is an established susceptibility gene for Parkinson’s disease (PD), one of the most common human neurodegenerative disorders. Increased SNCA is considered to lead to PD and dementia with Lewy bodies. Four single-nucleotide polymorphisms (SNPs) in SNCA 3Region were prominently associated with PD among different ethnic groups. To examine how these SNPs influence disease susceptibility, we analyzed their potential effects on SNCA gene expression. We found that rs356219 showed allele-specific features. Gel shift assay using nuclear extracts from SH-SY5Y cells showed binding of one or more proteins to the protective allele, rs356219-A. We purified the rs356219-A–protein complex with DNA affinity beads and identified a bound protein using mass spectrometry. This protein, YY1 (Yin Yang 1), is an ubiquitous transcription factor with multiple functions. We next investigated SNCA expression change in SH-SY5Y cells by YY1 transfection. We also analyzed the expression of antisense noncoding RNA (ncRNA) RP11-115D19.1 in SNCA 3′-Flanking Region, because rs356219 is located in intron of RP11-115D19.1 . Little change was observed in SNCA expression levels; however, RP11-115D19.1 expression was prominently stimulated by YY1. In autopsied cortices, positive correlation was observed among RP11-115D19.1 , SNCA and YY1 expression levels, suggesting their functional interactions in vivo . Knockdown of RP11-115D19.1 increased SNCA expression significantly in SH-SY5Y cells, suggesting its repressive effect on SNCA expression. Our findings of the protective allele-specific YY1 and antisense ncRNA raised a novel possible mechanism to regulate SNCA expression.

  • YY1 binds to α-synuclein 3′-Flanking Region SNP and stimulates antisense noncoding RNA expression
    Journal of Human Genetics, 2013
    Co-Authors: Ikuko Mizuta, Kazuaki Takafuji, Yuko Ando, Wataru Satake, Motoi Kanagawa, Kazuhiro Kobayashi, Shushi Nagamori, Takayuki Shinohara, Mitsutoshi Yamamoto

    Abstract:

    YY1 binds to α-synuclein 3′-Flanking Region SNP and stimulates antisense noncoding RNA expression

Mitsutoshi Yamamoto – 2nd expert on this subject based on the ideXlab platform

  • yy1 binds to α synuclein 3 Flanking Region snp and stimulates antisense noncoding rna expression
    Journal of Human Genetics, 2013
    Co-Authors: Ikuko Mizuta, Kazuaki Takafuji, Yuko Ando, Wataru Satake, Motoi Kanagawa, Kazuhiro Kobayashi, Shushi Nagamori, Takayuki Shinohara, Mitsutoshi Yamamoto

    Abstract:

    YY1 binds to α-synuclein 3′-Flanking Region SNP and stimulates antisense noncoding RNA expression

  • YY1 binds to α-synuclein 3′-Flanking Region SNP and stimulates antisense noncoding RNA expression
    Journal of Human Genetics, 2013
    Co-Authors: Ikuko Mizuta, Mitsutoshi Yamamoto, Kazuaki Takafuji, Yuko Ando, Wataru Satake, Motoi Kanagawa, Kazuhiro Kobayashi, Shushi Nagamori, Takayuki Shinohara, Nobutaka Hattori

    Abstract:

    α-synuclein ( SNCA ) is an established susceptibility gene for Parkinson’s disease (PD), one of the most common human neurodegenerative disorders. Increased SNCA is considered to lead to PD and dementia with Lewy bodies. Four single-nucleotide polymorphisms (SNPs) in SNCA 3Region were prominently associated with PD among different ethnic groups. To examine how these SNPs influence disease susceptibility, we analyzed their potential effects on SNCA gene expression. We found that rs356219 showed allele-specific features. Gel shift assay using nuclear extracts from SH-SY5Y cells showed binding of one or more proteins to the protective allele, rs356219-A. We purified the rs356219-A–protein complex with DNA affinity beads and identified a bound protein using mass spectrometry. This protein, YY1 (Yin Yang 1), is an ubiquitous transcription factor with multiple functions. We next investigated SNCA expression change in SH-SY5Y cells by YY1 transfection. We also analyzed the expression of antisense noncoding RNA (ncRNA) RP11-115D19.1 in SNCA 3′-Flanking Region, because rs356219 is located in intron of RP11-115D19.1 . Little change was observed in SNCA expression levels; however, RP11-115D19.1 expression was prominently stimulated by YY1. In autopsied cortices, positive correlation was observed among RP11-115D19.1 , SNCA and YY1 expression levels, suggesting their functional interactions in vivo . Knockdown of RP11-115D19.1 increased SNCA expression significantly in SH-SY5Y cells, suggesting its repressive effect on SNCA expression. Our findings of the protective allele-specific YY1 and antisense ncRNA raised a novel possible mechanism to regulate SNCA expression.

  • YY1 binds to α-synuclein 3′-Flanking Region SNP and stimulates antisense noncoding RNA expression
    Journal of Human Genetics, 2013
    Co-Authors: Ikuko Mizuta, Kazuaki Takafuji, Yuko Ando, Wataru Satake, Motoi Kanagawa, Kazuhiro Kobayashi, Shushi Nagamori, Takayuki Shinohara, Mitsutoshi Yamamoto

    Abstract:

    YY1 binds to α-synuclein 3′-Flanking Region SNP and stimulates antisense noncoding RNA expression

Philip L Huang – 3rd expert on this subject based on the ideXlab platform

  • the 3 Flanking Region of the human erythropoietin encoding gene contains nitrogen regulatory oxygen sensing consensus sequences and tissue specific transcriptional regulatory elements
    Gene, 1993
    Co-Authors: Leehuang Sylvia, Philip L Huang, Lin Jihjing, Kung Hsiangfu

    Abstract:

    Abstract We have reported the identification of a classical canonical CAAT box, TATA boxes and other transcriptional regulatory elements in the 5′ Flanking Region of the human erythropoietin (hEp)-encoding gene [Lee-Huang et al., Gene 128 (1993) 227–236]. These elements were not found in the hEp genomic clones reported by others. Our genomic clone extends in both directions beyond any reported clones, by 3.9 kb on the 5′ side and by 1.8 kb on the 3‘ side. Many important regulatory elements are found in these extended Flanking Regions. We report here the genomic structure of the extended 3Flanking Region of hEp. This Region contains the following regulatory elements: nitrogen-regulatory/oxygen-sensing consensus sequences, 5′-TTTTGCA and 5′-CCCTGCA; tissue-specific regulatory elements, including binding sites for A-activator, 5′-GTGGTGCAA; for DBP, 5′-TGATTTTGT; for HNF, 5′-T(A/G)TTTGT; and for C/EBP, 5′-T(T/G) (T/G)TGCAAT; a lymphokine-responsive element, 5′-GTGAAACCCC (Rev), as well as binding sites for AP and Spl. In addition, the nucleotide (nt) sequence in this Region is rich in inverted repeats (palindromes) that allow the formation of hairpin loops. A total of 14 potential stem loops with a maximum loop size of 20 nt are found. The identification of these regulatory elements in hEp should provide further insight into the tissue-specific and inducible expression of hEp. Such knowledge should be useful in the clinical modulation of erythropoiesis under physiologic and pathologic conditions.

  • The 3‘-Flanking Region of the human erythropoietin-encoding gene contains nitrogen-regulatory/oxygen-sensing consensus sequences and tissue-specific transcriptional regulatory elements
    Gene, 1993
    Co-Authors: Leehuang Sylvia, Philip L Huang, Lin Jih-jing, Kung Hsiang-fu

    Abstract:

    Abstract We have reported the identification of a classical canonical CAAT box, TATA boxes and other transcriptional regulatory elements in the 5′ Flanking Region of the human erythropoietin (hEp)-encoding gene [Lee-Huang et al., Gene 128 (1993) 227–236]. These elements were not found in the hEp genomic clones reported by others. Our genomic clone extends in both directions beyond any reported clones, by 3.9 kb on the 5′ side and by 1.8 kb on the 3‘ side. Many important regulatory elements are found in these extended Flanking Regions. We report here the genomic structure of the extended 3Flanking Region of hEp. This Region contains the following regulatory elements: nitrogen-regulatory/oxygen-sensing consensus sequences, 5′-TTTTGCA and 5′-CCCTGCA; tissue-specific regulatory elements, including binding sites for A-activator, 5′-GTGGTGCAA; for DBP, 5′-TGATTTTGT; for HNF, 5′-T(A/G)TTTGT; and for C/EBP, 5′-T(T/G) (T/G)TGCAAT; a lymphokine-responsive element, 5′-GTGAAACCCC (Rev), as well as binding sites for AP and Spl. In addition, the nucleotide (nt) sequence in this Region is rich in inverted repeats (palindromes) that allow the formation of hairpin loops. A total of 14 potential stem loops with a maximum loop size of 20 nt are found. The identification of these regulatory elements in hEp should provide further insight into the tissue-specific and inducible expression of hEp. Such knowledge should be useful in the clinical modulation of erythropoiesis under physiologic and pathologic conditions.