The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Michael Savin - One of the best experts on this subject based on the ideXlab platform.
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Greenfield filter fixation in large venae cavae.
Journal of vascular and interventional radiology : JVIR, 1998Co-Authors: Michael Savin, Richard D. Shlansky-goldbergAbstract:It is generally thought that the Greenfield filter should not be placed in inferior venae cavae (IVCs) that are larger than 28 mm in diameter because of its base diameter. However, the newer versions have larger base diameters. The purpose of this study was to evaluate fixation of the three currently available Greenfield filters in large IVCs. Filter fixation was tested in an ex vivo perfusion system with a 34-mm-diameter equine IVC. Greenfield filters with base diameters of 30 mm (original 24-F version [24-F GF]), 38 mm (percutaneous titanium [TGF]), and 32 mm (percutaneous stainless steel [SGF]) were deployed. Increasing force was then applied in a cephalic direction and the resultant movement was measured. In a 34-mm-diameter IVC, the TGF and SGF demonstrated significantly less movement than did the 24-F GF (P < .001). None of the TGFs or SGFs moved above the renal veins with a 480-g pull. Three of the seven 24-F GFs moved above the renal veins at 30 g. No significant difference in fixation was demonstrated between the TGF and the SGF (P = .6). In a 34-mm-diameter IVC, fixation of the TGF and SGF was significantly better than the 24-F GF. The TGF and SGF may not be subject to the same 28-mm-diameter IVC size limitation as the 24-F GF.
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greenfield filter fixation in large venae cavae
Journal of Vascular and Interventional Radiology, 1998Co-Authors: Michael Savin, Richard D ShlanskygoldbergAbstract:Purpose It is generally thought that the Greenfield filter should not be placed in inferior venae cavae (IVCs) that are larger than 28 mm in diameter because of its base diameter. However, the newer versions have larger base diameters. The purpose of this study was to evaluate fixation of the three currently available Greenfield filters in large IVCs. Materials and Methods Filter fixation was tested in an ex vivo perfusion system with a 34-mm-diameter equine IVC. Greenfield filters with base diameters of 30 mm (original 24-F version [24-F GF]), 38 mm (percutaneous titanium [TGF]), and 32 mm (percutaneous stainless steel [SGF]) were deployed. Increasing force was then applied in a cephalic direction and the resultant movement was measured. Results In a 34-mm-diameter IVC, the TGF and SGF demonstrated significantly less movement than did the 24-F GF ( P P =.6). Conclusions In a 34-mm-diameter IVC, fixation of the TGF and SGF was significantly better than the 24-F GF. The TGF and SGF may not be subject to the same 28-mm-diameter IVC size limitation as the 24-F GF.
Josef Havel - One of the best experts on this subject based on the ideXlab platform.
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formation of aluminium aluminium nitride and nitrogen clusters via laser ablation of nano aluminium nitride laser desorption ionisation and matrix assisted laser desorption ionisation time of flight mass spectrometry
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Nagender Reddy Panyala, Pavel Slavíček, Mirko Černák, Vadym Prysiazhnyi, Josef HavelAbstract:Laser Desorption Ionisation (LDI) and Matrix-Assisted Laser Desorption Ionisation (MALDI) Time-of-Flight Mass Spectrometry (TOFMS) were used to study the pulsed laser ablation of aluminium nitride (AlN) nano powder. The formation of Alm+ (m = 1–3), Nn+ (n = 4, 5), AlNn+ (n = 1–5, 19, 21), AlmN+ (m = 2–3), Al3N2+, Al9Nn+ (n = 5, 7, 9, 11 and 15), Al11Nn+ (n = 4, 6, 10, 12, 19, 21, 23, and 25), and Al13Nn+ (n = 25, 31, 32, 33, 34, 35, and 36) clusters was detected in positive ion mode. Similarly, Alm– (m = 1–3), AlNn– (n = 1–3, 5), AlmN– (m = 2, 3), Al2Nn– (n = 2–4, 28, 30), Nn– (n = 2, 3), Al4N7–, Al8Nn– (n = 1–6), and Al13Nn– (n = 9, 18, 20, 22, 24, 26, 28, 33, 35, 37, 39, 41 and 43) clusters were observed in negative ion mode. The formation of the stoichiometric Al10N10 cluster was shown to be of low abundance. On the contrary, the laser ablation of nano-AlN led mainly to the formation of nitrogen-rich AlmNn clusters in both negative and positive ion mode. The stoichiometry of the AlmNn clusters was determined via isotopic envelope analysis and computer modelling. Copyright © 2011 John Wiley & Sons, Ltd.
Mirko Černák - One of the best experts on this subject based on the ideXlab platform.
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formation of aluminium aluminium nitride and nitrogen clusters via laser ablation of nano aluminium nitride laser desorption ionisation and matrix assisted laser desorption ionisation time of flight mass spectrometry
Rapid Communications in Mass Spectrometry, 2011Co-Authors: Nagender Reddy Panyala, Pavel Slavíček, Mirko Černák, Vadym Prysiazhnyi, Josef HavelAbstract:Laser Desorption Ionisation (LDI) and Matrix-Assisted Laser Desorption Ionisation (MALDI) Time-of-Flight Mass Spectrometry (TOFMS) were used to study the pulsed laser ablation of aluminium nitride (AlN) nano powder. The formation of Alm+ (m = 1–3), Nn+ (n = 4, 5), AlNn+ (n = 1–5, 19, 21), AlmN+ (m = 2–3), Al3N2+, Al9Nn+ (n = 5, 7, 9, 11 and 15), Al11Nn+ (n = 4, 6, 10, 12, 19, 21, 23, and 25), and Al13Nn+ (n = 25, 31, 32, 33, 34, 35, and 36) clusters was detected in positive ion mode. Similarly, Alm– (m = 1–3), AlNn– (n = 1–3, 5), AlmN– (m = 2, 3), Al2Nn– (n = 2–4, 28, 30), Nn– (n = 2, 3), Al4N7–, Al8Nn– (n = 1–6), and Al13Nn– (n = 9, 18, 20, 22, 24, 26, 28, 33, 35, 37, 39, 41 and 43) clusters were observed in negative ion mode. The formation of the stoichiometric Al10N10 cluster was shown to be of low abundance. On the contrary, the laser ablation of nano-AlN led mainly to the formation of nitrogen-rich AlmNn clusters in both negative and positive ion mode. The stoichiometry of the AlmNn clusters was determined via isotopic envelope analysis and computer modelling. Copyright © 2011 John Wiley & Sons, Ltd.
Ruby C.y. Lin - One of the best experts on this subject based on the ideXlab platform.
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Identification of miR-34 regulatory networks in settings of disease and antimiR-therapy: Implications for treating cardiac pathology and other diseases
RNA Biology, 2016Co-Authors: Jenny Y. Y. Ooi, Bianca C. Bernardo, Saloni Singla, Natalie L. Patterson, Ruby C.y. Lin, Julie R. McmullenAbstract:Expression of the miR-34 family (miR-34a, -34b, -34c) is elevated in settings of heart disease, and inhibition with antimiR-34a/antimiR-34 has emerged as a promising therapeutic strategy. Under chronic cardiac disease settings, targeting the entire miR-34 family is more effective than targeting miR-34a alone. The identification of transcription factor (TF)-miRNA regulatory networks has added complexity to understanding the therapeutic potential of miRNA-based therapies. Here, we sought to determine whether antimiR-34 targets secondary miRNAs via TFs which could contribute to antimiR-34-mediated protection. Using miRNA-Seq we identified differentially regulated miRNAs in hearts from mice with cardiac pathology due to transverse aortic constriction (TAC), and focused on miRNAs which were also regulated by antimiR-34. Two clusters of stress-responsive miRNAs were classified as "pathological" and "cardioprotective," respectively. Using ChIPBase we identified 45 TF binding sites on the promoters of "pathological" and "cardioprotective" miRNAs, and 5 represented direct targets of miR-34, with the capacity to regulate other miRNAs. Knockdown studies in a cardiomyoblast cell line demonstrated that expression of 2 "pathological" miRNAs (let-7e, miR-31) was regulated by one of the identified TFs. Furthermore, by qPCR we confirmed that expression of let-7e and miR-31 was lower in hearts from antimiR-34 treated TAC mice; this may explain why targeting the entire miR-34 family is more effective than targeting miR-34a alone. Finally, we showed that Acsl4 (a common target of miR-34, let-7e and miR-31) was increased in hearts from TAC antimiR-34 treated mice. In summary, antimiR-34 regulates the expression of other miRNAs and this has implications for drug development.
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therapeutic inhibition of the mir 34 family attenuates pathological cardiac remodeling and improves heart function
Proceedings of the National Academy of Sciences of the United States of America, 2012Co-Authors: Bianca C. Bernardo, Xiaoming Gao, Catherine E Winbanks, Esther J H Boey, Yow Keat Tham, Helen Kiriazis, Paul Gregorevic, Susanna Obad, Sakari Kauppinen, Ruby C.y. LinAbstract:MicroRNAs are dysregulated in a setting of heart disease and have emerged as promising therapeutic targets. MicroRNA-34 family members (miR-34a, -34b, and -34c) are up-regulated in the heart in response to stress. In this study, we assessed whether inhibition of the miR-34 family using an s.c.-delivered seed-targeting 8-mer locked nucleic acid (LNA)-modified antimiR (LNA-antimiR-34) can provide therapeutic benefit in mice with preexisting pathological cardiac remodeling and dysfunction due to myocardial infarction (MI) or pressure overload via transverse aortic constriction (TAC). An additional cohort of mice subjected to MI was given LNA-antimiR-34a (15-mer) to inhibit miR-34a alone as a comparison for LNA-antimiR-34. LNA-antimiR-34 (8-mer) efficiently silenced all three miR-34 family members in both cardiac stress models and attenuated cardiac remodeling and atrial enlargement. In contrast, inhibition of miR-34a alone with LNA-antimiR-34a (15-mer) provided no benefit in the MI model. In mice subjected to pressure overload, LNA-antimiR-34 improved systolic function and attenuated lung congestion, associated with reduced cardiac fibrosis, increased angiogenesis, increased Akt activity, decreased atrial natriuretic peptide gene expression, and maintenance of sarcoplasmic reticulum Ca2+ ATPase gene expression. Improved outcome in LNA-antimiR-34–treated MI and TAC mice was accompanied by up-regulation of several direct miR-34 targets, including vascular endothelial growth factors, vinculin, protein O-fucosyltranferase 1, Notch1, and semaphorin 4B. Our results provide evidence that silencing of the entire miR-34 family can protect the heart against pathological cardiac remodeling and improve function. Furthermore, these data underscore the utility of seed-targeting 8-mer LNA-antimiRs in the development of new therapeutic approaches for pharmacologic inhibition of disease-implicated miRNA seed families.
Richard D Shlanskygoldberg - One of the best experts on this subject based on the ideXlab platform.
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greenfield filter fixation in large venae cavae
Journal of Vascular and Interventional Radiology, 1998Co-Authors: Michael Savin, Richard D ShlanskygoldbergAbstract:Purpose It is generally thought that the Greenfield filter should not be placed in inferior venae cavae (IVCs) that are larger than 28 mm in diameter because of its base diameter. However, the newer versions have larger base diameters. The purpose of this study was to evaluate fixation of the three currently available Greenfield filters in large IVCs. Materials and Methods Filter fixation was tested in an ex vivo perfusion system with a 34-mm-diameter equine IVC. Greenfield filters with base diameters of 30 mm (original 24-F version [24-F GF]), 38 mm (percutaneous titanium [TGF]), and 32 mm (percutaneous stainless steel [SGF]) were deployed. Increasing force was then applied in a cephalic direction and the resultant movement was measured. Results In a 34-mm-diameter IVC, the TGF and SGF demonstrated significantly less movement than did the 24-F GF ( P P =.6). Conclusions In a 34-mm-diameter IVC, fixation of the TGF and SGF was significantly better than the 24-F GF. The TGF and SGF may not be subject to the same 28-mm-diameter IVC size limitation as the 24-F GF.