The Experts below are selected from a list of 81 Experts worldwide ranked by ideXlab platform
Saima Shamim - One of the best experts on this subject based on the ideXlab platform.
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Synthesis and biological evaluation of schiff bases of 4-Aminophenazone as an anti-inflammatory, analgesic and antipyretic agent
Journal of Saudi Chemical Society, 2014Co-Authors: Shahzad Murtaza, Muhammad Shoaib Akhtar, Farina Kanwal, Aadil Abbas, Shoaib Ashiq, Saima ShamimAbstract:A series of schiff base derivatives of 4-Aminophenazone (4APZ-1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one) with different aldehydes were synthesized. The synthetic compounds were screened for their anti-inflammatory, analgesic and antipyretic activities. The characterization of synthesized compounds was carried out by 1H NMR, 13C NMR and MS. Carrageenan-induced paw oedema (CIPO) and histamine induced paw oedema (HIPO) methods were used to determine the anti-inflammatory activity of commercial sample of 4APZ and its synthesized schiff bases in mice. The anti-inflammatory activity was in the order of 4APZAB > 4APZBB > 4APZCB > 4APZVn and all the test compounds exhibited considerable dose dependent inhibition of the paw oedema. The effect of the compounds on membrane stabilization was also determined which showed that compounds 4APZ (120 and 240 mg/kg doses), 4APZAB (160 mg/kg) and 4APZVn (600 mg/kg) produced highly significant inhibition (P 4APZBB > 4APZVn > 4APZCB. Moreover, phenobarbitone-induced sleeping time (PIST) in mice was also studied but only 600 mg/kg of 4APZVn significantly increased the duration of induced sleep which also suggested its sedative property. Brewer’s yeast was used to induce fever in rabbits and analysed the compounds for their antipyretic activity. Different doses of 4APZ for different time durations (240 mg/kg-after 1 h, 120 and 240 mg/kg doses-after 2 h) produced highly significant (P < 0.001) inhibition of hyperpyrexia. Other compounds showed good antipyretic activity after 2, 3 and 4 h.
Sharanappa T Nandibewoor - One of the best experts on this subject based on the ideXlab platform.
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simultaneous electrochemical determination of 4 Aminophenazone and caffeine at electrochemically pre treated graphite pencil electrode
Analytical Methods, 2014Co-Authors: Jayant I Gowda, Sharanappa T NandibewoorAbstract:A new and sensitive electroanalytical method for simultaneous determination of selected drugs, 4-Aminophenazone (4-AP) and caffeine (CAF) has been developed and validated. Cyclic voltammetry (CV) was used to study the electrochemical behavior of the drugs, while differential pulse voltammetry (DPV) was used to determine 4-AP and CAF simultaneously. A pre-treated graphite pencil electrode (PTGPE) was used as the working electrode, a Ag/AgCl (3.0 M KCl) electrode served as the reference electrode, and a platinum wire as the auxiliary electrode. Determination of drugs was performed in phosphate buffer solution (PBS) of pH = 3.0. The separation of the oxidation peak potentials for 4-AP–CAF was found to be 0.930 V. This difference was large enough to determine 4-AP and CAF individually and simultaneously. The dependence of the current on pH, concentration and scan rate was investigated to optimize the experimental conditions for simultaneous determination. The calibration plots for both the drugs were linear in certain concentration ranges. The linearity range for 4-AP was 1 μM to 11 μM and for CAF 1 μM to 9 μM, the concentration of each drug was varied by keeping the other constant, and achieved lower detection limit of 7.96 × 10−8 M for 4-AP and 9.81 × 10−9 M for CAF. The developed method was found to be precise, selective and rapid for the simultaneous determination of 4-AP and CAF. The proposed method has been applied for the determination of 4-AP and CAF in real samples.
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electrochemical behavior of 4 Aminophenazone drug at a graphite pencil electrode and its application in real samples
Industrial & Engineering Chemistry Research, 2012Co-Authors: Jayant I Gowda, Sharanappa T NandibewoorAbstract:An analytical method for the determination of 4-Aminophenazone using cyclic and differential-pulse voltammetry has been developed. The oxidation process was shown to be irreversible over the pH range 3.0―11.2. The dependence of the current on pH, concentration, and scan rate was investigated to optimize the experimental conditions for the determination of 4-Aminophenazone. The number of electrons transferred in the oxidation process was calculated, and a probable oxidation mechanism was proposed. In the range of ( 1.0 X 10 ―6 )―(1.6 X 10 ―5 ) M, the current measured by differential-pulse voltammetry as a function of the concentration of 4-Aminophenazone with a detection limit of 0.458 × 10 ―7 M with good selectivity and sensitivity exhibited a good linear relationship. The proposed method was applied to the determination of 4-Aminophenazone in a real sample.
Shahzad Murtaza - One of the best experts on this subject based on the ideXlab platform.
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Synthesis and biological evaluation of schiff bases of 4-Aminophenazone as an anti-inflammatory, analgesic and antipyretic agent
Journal of Saudi Chemical Society, 2014Co-Authors: Shahzad Murtaza, Muhammad Shoaib Akhtar, Farina Kanwal, Aadil Abbas, Shoaib Ashiq, Saima ShamimAbstract:A series of schiff base derivatives of 4-Aminophenazone (4APZ-1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one) with different aldehydes were synthesized. The synthetic compounds were screened for their anti-inflammatory, analgesic and antipyretic activities. The characterization of synthesized compounds was carried out by 1H NMR, 13C NMR and MS. Carrageenan-induced paw oedema (CIPO) and histamine induced paw oedema (HIPO) methods were used to determine the anti-inflammatory activity of commercial sample of 4APZ and its synthesized schiff bases in mice. The anti-inflammatory activity was in the order of 4APZAB > 4APZBB > 4APZCB > 4APZVn and all the test compounds exhibited considerable dose dependent inhibition of the paw oedema. The effect of the compounds on membrane stabilization was also determined which showed that compounds 4APZ (120 and 240 mg/kg doses), 4APZAB (160 mg/kg) and 4APZVn (600 mg/kg) produced highly significant inhibition (P 4APZBB > 4APZVn > 4APZCB. Moreover, phenobarbitone-induced sleeping time (PIST) in mice was also studied but only 600 mg/kg of 4APZVn significantly increased the duration of induced sleep which also suggested its sedative property. Brewer’s yeast was used to induce fever in rabbits and analysed the compounds for their antipyretic activity. Different doses of 4APZ for different time durations (240 mg/kg-after 1 h, 120 and 240 mg/kg doses-after 2 h) produced highly significant (P < 0.001) inhibition of hyperpyrexia. Other compounds showed good antipyretic activity after 2, 3 and 4 h.
Jayant I Gowda - One of the best experts on this subject based on the ideXlab platform.
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simultaneous electrochemical determination of 4 Aminophenazone and caffeine at electrochemically pre treated graphite pencil electrode
Analytical Methods, 2014Co-Authors: Jayant I Gowda, Sharanappa T NandibewoorAbstract:A new and sensitive electroanalytical method for simultaneous determination of selected drugs, 4-Aminophenazone (4-AP) and caffeine (CAF) has been developed and validated. Cyclic voltammetry (CV) was used to study the electrochemical behavior of the drugs, while differential pulse voltammetry (DPV) was used to determine 4-AP and CAF simultaneously. A pre-treated graphite pencil electrode (PTGPE) was used as the working electrode, a Ag/AgCl (3.0 M KCl) electrode served as the reference electrode, and a platinum wire as the auxiliary electrode. Determination of drugs was performed in phosphate buffer solution (PBS) of pH = 3.0. The separation of the oxidation peak potentials for 4-AP–CAF was found to be 0.930 V. This difference was large enough to determine 4-AP and CAF individually and simultaneously. The dependence of the current on pH, concentration and scan rate was investigated to optimize the experimental conditions for simultaneous determination. The calibration plots for both the drugs were linear in certain concentration ranges. The linearity range for 4-AP was 1 μM to 11 μM and for CAF 1 μM to 9 μM, the concentration of each drug was varied by keeping the other constant, and achieved lower detection limit of 7.96 × 10−8 M for 4-AP and 9.81 × 10−9 M for CAF. The developed method was found to be precise, selective and rapid for the simultaneous determination of 4-AP and CAF. The proposed method has been applied for the determination of 4-AP and CAF in real samples.
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electrochemical behavior of 4 Aminophenazone drug at a graphite pencil electrode and its application in real samples
Industrial & Engineering Chemistry Research, 2012Co-Authors: Jayant I Gowda, Sharanappa T NandibewoorAbstract:An analytical method for the determination of 4-Aminophenazone using cyclic and differential-pulse voltammetry has been developed. The oxidation process was shown to be irreversible over the pH range 3.0―11.2. The dependence of the current on pH, concentration, and scan rate was investigated to optimize the experimental conditions for the determination of 4-Aminophenazone. The number of electrons transferred in the oxidation process was calculated, and a probable oxidation mechanism was proposed. In the range of ( 1.0 X 10 ―6 )―(1.6 X 10 ―5 ) M, the current measured by differential-pulse voltammetry as a function of the concentration of 4-Aminophenazone with a detection limit of 0.458 × 10 ―7 M with good selectivity and sensitivity exhibited a good linear relationship. The proposed method was applied to the determination of 4-Aminophenazone in a real sample.
Humaira Nadeem - One of the best experts on this subject based on the ideXlab platform.
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spectroscopic electrochemical dna binding and in vivo anti inflammatory studies on newly synthesized schiff bases of 4 Aminophenazone
Journal of Photochemistry and Photobiology B-biology, 2014Co-Authors: Nasima Arshad, Mukhtar Ahmad, Muhammad Zaman Ashraf, Humaira NadeemAbstract:4-Aminophenazone (Ap-1) Schiff bases i.e., 4-{(3,4,5-trimethoxybenzylidine) amino}phenazone (Ap-2), 4-{(2-chlorobenzylidine) amino}phenazone (Ap-3) and 4-{(4-chlorobenzylidine)amino} phenazone (Ap-4) were synthesized and characterized by different spectroscopic techniques. Interaction of these compounds with ds.DNA was investigated through UV-Visible spectroscopy, fluorescence spectroscopy and cyclic voltammetry at stomach (4.7) and blood (7.4) pH under 37 °C (human body temperature). Instrumental findings were further quantified both kinetically and thermodynamically. Results obtained through these techniques inferred intercalative mode of binding of all the compounds with DNA. The binding constant data, "Kb", and free energy change, ΔG, indicated comparatively greater binding affinity and more spontaneity of binding of compounds with DNA at stomach pH (4.7), respectively. However, among these compounds, Ap-4 showed comparatively greater binding at both the pH. Formation of compound-DNA complex was further confirmed through the decrease in diffusion rates after the addition of DNA. The in vivo anti-inflammatory activity of the compounds was evaluated using the carrageenan-induced hind paw edema method. The results revealed that among all the compounds, Ap-4 showed greater percentage of edema inhibition compared to standard drug.
