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5 Flanking Region
The Experts below are selected from a list of 40080 Experts worldwide ranked by ideXlab platform
Norio Kaneda – One of the best experts on this subject based on the ideXlab platform.
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Characterization of the 5′-Flanking Region of the rat TIS11 gene
Molecular and Cellular Biochemistry, 2000Co-Authors: Tomiyasu Murata, Yoshiro Niimi, Norio KanedaAbstract:To understand the mechanism underlying the transcriptional regulation of the immediate early gene TIS11, we characterized the 5′-Flanking Region of the rat TIS11 gene. When fused to the luciferase reporter gene, the 5.3-kb 5′-Flanking Region of the rat TIS11 gene exhibited functional promoter activity in pheochromocytoma PC12 and hepatoma H4IIE cells. 5′-Deletion analyses indicated that multiple negative and positive regulatory Regions were present in the 5′-Flanking Region, and that some of these Regions functioned in a cell type-specific manner. Promoter activity of the rat TIS11 gene was enhanced by phorbol 12-myristate 13-acetate (PMA) in both cell lines, and the PMA-responsiveness resided within the 5′-Flanking Region. The induction of promoter activity by PMA was completely blocked by GF109203X or PD98059, inhibitors of protein kinase C and mitogen-activated protein (MAP) kinase kinase, respectively. These results suggested that induction of the rat TIS11 promoter by PMA is mediated by activation of the protein kinase C/MAP kinase cascade.
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Characterization of the 5‘-Flanking Region of the rat TIS11 gene.
Molecular and cellular biochemistry, 2000Co-Authors: Tomiyasu Murata, Yoshiro Niimi, Norio KanedaAbstract:To understand the mechanism underlying the transcriptional regulation of the immediate early gene TIS11, we characterized the 5‘-Flanking Region of the rat TIS11 gene. When fused to the luciferase reporter gene, the 5.3-kb 5‘-Flanking Region of the rat TIS11 gene exhibited functional promoter activity in pheochromocytoma PC12 and hepatoma H4IIE cells. 5‘-Deletion analyses indicated that multiple negative and positive regulatory Regions were present in the 5‘-Flanking Region, and that some of these Regions functioned in a cell type-specific manner. Promoter activity of the rat TIS 11 gene was enhanced by phorbol 12-myristate 13-acetate (PMA) in both cell lines, and the PMA-responsiveness resided within the 5‘-Flanking Region. The induction of promoter activity by PMA was completely blocked by GF109203X or PD98059, inhibitors of protein kinase C and mitogen-activated protein (MAP) kinase kinase, respectively. These results suggested that induction of the rat TIS 11 promoter by PMA is mediated by activation of the protein kinase C/MAP kinase cascade.
Tomiyasu Murata – One of the best experts on this subject based on the ideXlab platform.
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Characterization of the 5′-Flanking Region of the rat TIS11 gene
Molecular and Cellular Biochemistry, 2000Co-Authors: Tomiyasu Murata, Yoshiro Niimi, Norio KanedaAbstract:To understand the mechanism underlying the transcriptional regulation of the immediate early gene TIS11, we characterized the 5′-Flanking Region of the rat TIS11 gene. When fused to the luciferase reporter gene, the 5.3-kb 5′-Flanking Region of the rat TIS11 gene exhibited functional promoter activity in pheochromocytoma PC12 and hepatoma H4IIE cells. 5′-Deletion analyses indicated that multiple negative and positive regulatory Regions were present in the 5′-Flanking Region, and that some of these Regions functioned in a cell type-specific manner. Promoter activity of the rat TIS11 gene was enhanced by phorbol 12-myristate 13-acetate (PMA) in both cell lines, and the PMA-responsiveness resided within the 5′-Flanking Region. The induction of promoter activity by PMA was completely blocked by GF109203X or PD98059, inhibitors of protein kinase C and mitogen-activated protein (MAP) kinase kinase, respectively. These results suggested that induction of the rat TIS11 promoter by PMA is mediated by activation of the protein kinase C/MAP kinase cascade.
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Characterization of the 5‘-Flanking Region of the rat TIS11 gene.
Molecular and cellular biochemistry, 2000Co-Authors: Tomiyasu Murata, Yoshiro Niimi, Norio KanedaAbstract:To understand the mechanism underlying the transcriptional regulation of the immediate early gene TIS11, we characterized the 5‘-Flanking Region of the rat TIS11 gene. When fused to the luciferase reporter gene, the 5.3-kb 5‘-Flanking Region of the rat TIS11 gene exhibited functional promoter activity in pheochromocytoma PC12 and hepatoma H4IIE cells. 5‘-Deletion analyses indicated that multiple negative and positive regulatory Regions were present in the 5‘-Flanking Region, and that some of these Regions functioned in a cell type-specific manner. Promoter activity of the rat TIS 11 gene was enhanced by phorbol 12-myristate 13-acetate (PMA) in both cell lines, and the PMA-responsiveness resided within the 5‘-Flanking Region. The induction of promoter activity by PMA was completely blocked by GF109203X or PD98059, inhibitors of protein kinase C and mitogen-activated protein (MAP) kinase kinase, respectively. These results suggested that induction of the rat TIS 11 promoter by PMA is mediated by activation of the protein kinase C/MAP kinase cascade.
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Binding of kidney nuclear proteins to the 5′-Flanking Region of the rat gene for Ca2+-binding protein regucalcin: Involvement of Ca2+/calmodulin signaling
Molecular and Cellular Biochemistry, 1999Co-Authors: Tomiyasu Murata, Masayoshi YamaguchiAbstract:Ca2+-binding protein regucalcin is expressed in the kidney cortex of rats, as assayed by Northern blot analysis. The existence of kidney nuclear factor which binds to the 5‘-Flanking Region of the rat regucalcin gene was investigated. When nuclear extracts obtained from the kidney cortex of rats were used in gel mobility-shift assays, two protein-DNA complexes were uniquely formed with the DNA fragment containing the 5‘-Flanking Region of the rat regucalcin gene. Competition gel shift experiments indicated the specific binding Region of kidney cortex nuclear proteins in the 5′-Flanking Region of the rat regucalcin gene. The two nuclear protein-DNA complexes were formed with the same mobility in rat kidney cortex and liver, which possess detectable amounts of regucalcin mRNA in Northern blot analysis. The binding activities of nuclear factors from kidney cortex to the 5′-Flanking Region of the rat regucalcin gene were inhibited by a single intraperitoneal administration of trifluoperazine, an antagonist of calmodulin, to rats. The present study demonstrates that kidney cortex nuclear proteins specifically bind to the 5′-Flanking Region of the rat regucalcin gene, and that the binding activity may be partly mediated through the Ca2+/calmodulin-dependent process.
Yoshiro Niimi – One of the best experts on this subject based on the ideXlab platform.
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Characterization of the 5′-Flanking Region of the rat TIS11 gene
Molecular and Cellular Biochemistry, 2000Co-Authors: Tomiyasu Murata, Yoshiro Niimi, Norio KanedaAbstract:To understand the mechanism underlying the transcriptional regulation of the immediate early gene TIS11, we characterized the 5′-Flanking Region of the rat TIS11 gene. When fused to the luciferase reporter gene, the 5.3-kb 5′-Flanking Region of the rat TIS11 gene exhibited functional promoter activity in pheochromocytoma PC12 and hepatoma H4IIE cells. 5′-Deletion analyses indicated that multiple negative and positive regulatory Regions were present in the 5′-Flanking Region, and that some of these Regions functioned in a cell type-specific manner. Promoter activity of the rat TIS11 gene was enhanced by phorbol 12-myristate 13-acetate (PMA) in both cell lines, and the PMA-responsiveness resided within the 5′-Flanking Region. The induction of promoter activity by PMA was completely blocked by GF109203X or PD98059, inhibitors of protein kinase C and mitogen-activated protein (MAP) kinase kinase, respectively. These results suggested that induction of the rat TIS11 promoter by PMA is mediated by activation of the protein kinase C/MAP kinase cascade.
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Characterization of the 5‘-Flanking Region of the rat TIS11 gene.
Molecular and cellular biochemistry, 2000Co-Authors: Tomiyasu Murata, Yoshiro Niimi, Norio KanedaAbstract:To understand the mechanism underlying the transcriptional regulation of the immediate early gene TIS11, we characterized the 5‘-Flanking Region of the rat TIS11 gene. When fused to the luciferase reporter gene, the 5.3-kb 5‘-Flanking Region of the rat TIS11 gene exhibited functional promoter activity in pheochromocytoma PC12 and hepatoma H4IIE cells. 5‘-Deletion analyses indicated that multiple negative and positive regulatory Regions were present in the 5‘-Flanking Region, and that some of these Regions functioned in a cell type-specific manner. Promoter activity of the rat TIS 11 gene was enhanced by phorbol 12-myristate 13-acetate (PMA) in both cell lines, and the PMA-responsiveness resided within the 5‘-Flanking Region. The induction of promoter activity by PMA was completely blocked by GF109203X or PD98059, inhibitors of protein kinase C and mitogen-activated protein (MAP) kinase kinase, respectively. These results suggested that induction of the rat TIS 11 promoter by PMA is mediated by activation of the protein kinase C/MAP kinase cascade.