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Sylvain Huchette - One of the best experts on this subject based on the ideXlab platform.
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protective efficacy of a pseudoalteromonas strain in european Abalone haliotis tuberculata infected with vibrio harveyi orm4
Probiotics and Antimicrobial Proteins, 2019Co-Authors: Clement Offret, Sylvain Huchette, Vincent Rochard, Helene Laguerre, Jerome Mounier, Benjamin Brillet, Patrick Le Chevalier, Yannick FleuryAbstract:: The hemolymph of healthy marine invertebrates is known to harbor antibiotic-producing bacteria belonging to the genus Pseudoalteromonas. Such strains are potential probiotics to control infectious diseases in aquaculture. In the present study, we screened a collection of Pseudoalteromonas strains isolated from the hemolymph of oyster and mussel for antimicrobial activity against Vibrio harveyi, a pathogenic species responsible for high mortality in Abalone. Subsequently, the protective efficacy of the most active strain named hCg-6 was investigated in Abalone culture faced with a Vibrio harveyi ORM4 infection. First, we have controlled the Pseudoalteromonas hCg-6 safety for Abalone health. To that end, animals were immersed for 4 h in Pseudoalteromonas hCg-6 suspensions in seawater. The Abalone viability was monitored and Pseudoalteromonas hCg-6 was tracked by quantitative-PCR in Abalone hemolymph. After immersion, no Abalone death occurred while the strain hCg-6 was significantly detected in hemolymph. Therefore, the strain hCg-6 was considered safe for Abalone and evaluated for its ability to protect Abalone against V. harveyi (injection of 1 × 103Vibrio per animal). A 4-h long immersion of Abalone in a seawater suspension of Pseudoalteromonas hCg-6 (1 × 106 CFU mL-1) prior to infection with Vibrio harveyi significantly improved the Abalone viability. Indeed, 15 days post infection, the hCg-6 treatment used increased the Abalone survival rate from 16% in untreated animals to 40% in treated Abalone. We hypothesized that Pseudoalteromonas hCg-6 antibacterial activity increased the hemomicrobiota shielding effect. In conclusion, Pseudoalteromonas hCg-6 is a promising anti-Vibrio strain for Abalone culture.
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relationships between growth survival physiology and behaviour a multi criteria approach to haliotis tuberculata phenotypic traits
Aquaculture, 2017Co-Authors: Sebastien Lachambre, Sylvain Huchette, Antoine Riocabello, Timothee Fustec, Pierre Boudry, Sabine RousselAbstract:Abalone growth rate is often identified among important traits to improve through selective breeding. However, the rapid success of some selective breeding plans has sometimes led to negative effects in some aquaculture species due to trade-offs. One of them is the loss of homeostasis of selected animals which results in the inability to resist the stress experienced during the rearing process. In this context, this study aimed to analyze the phenotypic relationships between growth, and physiological and behavioural traits in Haliotis tuberculata under stressful conditions. Eleven traits related to growth, immunity, reproduction and behaviour were recorded under laboratory conditions. A total of 120 adults from wild or farm origin were first monitored during a 3-week stress period (high density and acute stress handling) during winter, followed by 6 months on-growing in sea-cages. Relationships between parameters were analyzed using a multi-factorial approach. Wild and farm stocks could only be discriminated on behaviour traits, confirming that the French Abalone industry is in the beginning of the domestication process. After 3 weeks of chronic stress, the righting latency of an Abalone was linked to better survival and faster growth. Abalones having the best growth after 6 months were characterized by higher activity during the previous winter period, whereas an early gonad maturation reduced the growth in summer. Our results provide a basis for the establishment of a multi-trait breeding program to improve the growth rate while controlling the evolution of physiological and behavioural traits.ăStatement of relevanceăThe relationships of behavioural and physiological variables with survival and weight gain after application of important stressors were studied in order to provide a better understanding of Haliotis tuberculata biology during early domestication stage. This paper will give information on new targets and tools for selective breeding.
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Relationships between growth, survival, physiology and behaviour — A multi-criteria approach to Haliotis tuberculata phenotypic traits
Aquaculture, 2017Co-Authors: Sebastien Lachambre, Sylvain Huchette, Timothee Fustec, Antoine Rio-cabello, Pierre Boudry, Sabine RousselAbstract:Abalone growth rate is often identified among important traits to improve through selective breeding. However, the rapid success of some selective breeding plans has sometimes led to negative effects in some aquaculture species due to trade-offs. One of them is the loss of homeostasis of selected animals which results in the inability to resist the stress experienced during the rearing process. In this context, this study aimed to analyze the phenotypic relationships between growth, and physiological and behavioural traits in Haliotis tuberculata under stressful conditions. Eleven traits related to growth, immunity, reproduction and behaviour were recorded under laboratory conditions. A total of 120 adults from wild or farm origin were first monitored during a 3-week stress period (high density and acute stress handling) during winter, followed by 6 months on-growing in sea-cages. Relationships between parameters were analyzed using a multi-factorial approach. Wild and farm stocks could only be discriminated on behaviour traits, confirming that the French Abalone industry is in the beginning of the domestication process. After 3 weeks of chronic stress, the righting latency of an Abalone was linked to better survival and faster growth. Abalones having the best growth after 6 months were characterized by higher activity during the previous winter period, whereas an early gonad maturation reduced the growth in summer. Our results provide a basis for the establishment of a multi-trait breeding program to improve the growth rate while controlling the evolution of physiological and behavioural traits.ăStatement of relevanceăThe relationships of behavioural and physiological variables with survival and weight gain after application of important stressors were studied in order to provide a better understanding of Haliotis tuberculata biology during early domestication stage. This paper will give information on new targets and tools for selective breeding.
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the ormer haliotis tuberculata a new promising paleoclimatic tool
Palaeogeography Palaeoclimatology Palaeoecology, 2015Co-Authors: Aurelie Jolivet, Sylvain Huchette, Clement Legoff, Julien Thebault, Kamal Nasreddine, Bernd R Schone, Laurent Chauvaud, Jacques ClavierAbstract:Abstract This study aimed to investigate the environmental controls on the oxygen isotope composition of shells of the European Abalone, Haliotis tuberculata. Seasonal δ18O profiles from the outer prismatic layer of four Abalone shells, collected live in northwest Brittany (France) in 2002 and 2012, were compared to local temperatures and salinities. According to the findings herein, δ18O variations in Abalone shells corresponded to seasonal variations, and thus, shell composition represented a reliable tool for aging and growth studies. Seawater temperatures estimated from the Abalone collected in 2012 reflected the in situ measured temperatures, but the reconstructed temperatures from shells of the three specimens collected in 2002 deviated from measured temperatures by 2.5 °C. This overestimation of temperatures corresponded to a “kinetic effect” related to very high annual Abalone growth rates; thus, it could be corrected by applying + 0.53‰ to the δ18Oshell. This methodology was then applied to a fossil (6000 cal yr BP) collected in the Bay of Biscay. Given the worldwide distribution of both live and fossilized Abalones, the results of the present study showed that this genus represents a promising paleoclimatic tool.
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shell growth microstructure and composition over the development cycle of the european Abalone haliotis tuberculata
Marine Biology, 2015Co-Authors: Stephanie Auzouxbordenave, Aicha Badou, C Brahmi, Marc De Rafélis, Sylvain HuchetteAbstract:The shell of the European Abalone Haliotis tuberculata is a model for studying mechanisms of mollusc shell formation, but the early steps of shell formation and calcification remain poorly documented. The microstructure and the mineralogical and geochemical composition of larval and juvenile shells were investigated by scanning electron microscopy, infrared spectroscopy and ion microprobe analyses (NanoSIMS). Analyses were performed on shells obtained from controlled fertilisations at the hatchery France-Haliotis (Plouguerneau, France) in July 2009 and 2010 using Abalone from Roscoff. Shell cross sections revealed the microstructural arrangement of the developing shell, showing progressive biomineral organisation into two differentiated layers, i.e. the outer granular and the internal nacreous layer. Infrared analysis confirmed that the European Abalone shell, at every stage of development, was mostly composed of CaCO3 in the form of aragonite. Variations in trace element composition, i.e. Sr/Ca, were measured in the different stages and correlated with micro-structural changes in the shells. Experimental manganese labelling of live Abalones produced cathodoluminescence marks in the growing shell sections. The increase in shell thickness can be used to determine the growth rate of an early adult Abalone shell.
Christine Paillard - One of the best experts on this subject based on the ideXlab platform.
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vibrio harveyi adheres to and penetrates tissues of the european Abalone haliotis tuberculata within the first hours of contact
Applied and Environmental Microbiology, 2014Co-Authors: Marion Cardinaud, Annaick Barbou, Carole Capitaine, Antoine M Dujon, Dario Moraga, Adeline Bidault, Christine PaillardAbstract:Vibrio harveyi is a marine bacterial pathogen responsible for episodic epidemics generally associated with massive mortalities in many marine organisms, including the European Abalone Haliotis tuberculata. The aim of this study was to identify the portal of entry and the dynamics of infection of V. harveyi in the European Abalone. The results indicate that the duration of contact be-tween V. harveyi and the European Abalone influences the mortality rate and precocity. Immediately after contact, the epithelial and mucosal area situated between the gills and the hypobranchial gland was colonized by V. harveyi. Real-time PCR analyses and culture quantification of a green fluorescent protein-tagged strain of V. harveyi in Abalone tissues revealed a high density of bacteria adhering to and then penetrating the whole gill-hypobranchial gland tissue after 1 h of contact. V. harveyi was also de-tected in the hemolymph of a significant number of European Abalones after 3 h of contact. In conclusion, this article shows that a TaqMan real-time PCR assay is a powerful and useful technique for the detection of a marine pathogen such as V. harveyi in mollusk tissue and for the study of its infection dynamics. Thus, we have revealed that the adhesion and then the penetration of V. harveyi in European Abalone organs begin in the first hours of contact. We also hypothesize that the portal of entry of V. har-veyi in the European Abalone is the area situated between the gills and the hypobranchial gland.
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Characterization of Abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures
Cytotechnology, 2013Co-Authors: Delphine Pichon, Benoit Cudennec, Sylvain Huchette, Chakib Djediat, Tristan Renault, Christine Paillard, Stéphanie Auzoux-bordenaveAbstract:The decline of European Abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio . Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund Abalones, allowing in vivo and in vitro studies on the interactions between Abalone H. tuberculata and V. harveyi . This work reports the development of primary cell cultures from Abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell— V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days. Cytological parameters, cell morphology and viability were monitored over time using flow cytometry analysis and semi-quantitative assay (XTT). Then, gill cell cultures were used to investigate in vitro the interactions with V. harveyi . The effects of two bacterial strains were evaluated on gill cells: a pathogenic bacterial strain ORM4 which is responsible for Abalone mortalities and LMG7890 which is a non-pathogenic strain. Cellular responses of gill cells exposed to increasing concentrations of bacteria were evaluated by measuring mitochondrial activity (XTT assay) and phenoloxidase activity, an enzyme which is strongly involved in immune response. The ability of gill cells to phagocyte GFP-tagged V. harveyi was evaluated by flow cytometry and gill cells- V. harveyi interactions were characterized using fluorescence microscopy and transmission electron microscopy. During phagocytosis process we evidenced that V. harveyi bacteria induced significant changes in gill cells metabolism and immune response. Together, the results showed that primary cell cultures from Abalone gills are suitable for in vitro study of host-pathogen interactions, providing complementary assays to in vivo experiments.
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Characterization of Abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures
Cytotechnology, 2013Co-Authors: Delphine Pichon, Benoit Cudennec, Sylvain Huchette, Chakib Djediat, Tristan Renault, Christine Paillard, Stéphanie Auzoux-bordenaveAbstract:The decline of European Abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund Abalones, allowing in vivo and in vitro studies on the interactions between Abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from Abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell-V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days. Cytological parameters, cell morphology and viability were monitored over time using flow cytometry analysis and semi-quantitative assay (XTT). Then, gill cell cultures were used to investigate in vitro the interactions with V. harveyi. The effects of two bacterial strains were evaluated on gill cells: a pathogenic bacterial strain ORM4 which is responsible for Abalone mortalities and LMG7890 which is a non-pathogenic strain. Cellular responses of gill cells exposed to increasing concentrations of bacteria were evaluated by measuring mitochondrial activity (XTT assay) and phenoloxidase activity, an enzyme which is strongly involved in immune response. The ability of gill cells to phagocyte GFP-tagged V. harveyi was evaluated by flow cytometry and gill cells-V. harveyi interactions were characterized using fluorescence microscopy and transmission electron microscopy. During phagocytosis process we evidenced that V. harveyi bacteria induced significant changes in gill cells metabolism and immune response. Together, the results showed that primary cell cultures from Abalone gills are suitable for in vitro study of host-pathogen interactions, providing complementary assays to in vivo experiments.
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Influence of temperature and spawning effort on Haliotis tuberculata mortalities caused by Vibrio harveyi: an example of emerging vibriosis linked to global warming
Global Change Biology, 2009Co-Authors: Marie-agnès Travers, Sylvain Huchette, Olivier Basuyaux, Nelly Le Goïc, Marcel Koken, Jeanlouis Nicolas, Christine PaillardAbstract:Since 1998, Haliotis tuberculata mass mortalities have been occurring regularly in wild Abalone populations in France during their reproductive period and in conjunction with seawater summer temperature maxima and Vibrio harveyi presence. To confirm the importance of bacterial exposure, temperature and reproductive status on Abalone susceptibility, experimental infections via bath exposure were performed using Abalone ranging from immature to reproductively mature. Ripe Abalone were more susceptible to the bacterium than immature specimens (P
Sabine Roussel - One of the best experts on this subject based on the ideXlab platform.
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relationships between growth survival physiology and behaviour a multi criteria approach to haliotis tuberculata phenotypic traits
Aquaculture, 2017Co-Authors: Sebastien Lachambre, Sylvain Huchette, Antoine Riocabello, Timothee Fustec, Pierre Boudry, Sabine RousselAbstract:Abalone growth rate is often identified among important traits to improve through selective breeding. However, the rapid success of some selective breeding plans has sometimes led to negative effects in some aquaculture species due to trade-offs. One of them is the loss of homeostasis of selected animals which results in the inability to resist the stress experienced during the rearing process. In this context, this study aimed to analyze the phenotypic relationships between growth, and physiological and behavioural traits in Haliotis tuberculata under stressful conditions. Eleven traits related to growth, immunity, reproduction and behaviour were recorded under laboratory conditions. A total of 120 adults from wild or farm origin were first monitored during a 3-week stress period (high density and acute stress handling) during winter, followed by 6 months on-growing in sea-cages. Relationships between parameters were analyzed using a multi-factorial approach. Wild and farm stocks could only be discriminated on behaviour traits, confirming that the French Abalone industry is in the beginning of the domestication process. After 3 weeks of chronic stress, the righting latency of an Abalone was linked to better survival and faster growth. Abalones having the best growth after 6 months were characterized by higher activity during the previous winter period, whereas an early gonad maturation reduced the growth in summer. Our results provide a basis for the establishment of a multi-trait breeding program to improve the growth rate while controlling the evolution of physiological and behavioural traits.ăStatement of relevanceăThe relationships of behavioural and physiological variables with survival and weight gain after application of important stressors were studied in order to provide a better understanding of Haliotis tuberculata biology during early domestication stage. This paper will give information on new targets and tools for selective breeding.
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Relationships between growth, survival, physiology and behaviour — A multi-criteria approach to Haliotis tuberculata phenotypic traits
Aquaculture, 2017Co-Authors: Sebastien Lachambre, Sylvain Huchette, Timothee Fustec, Antoine Rio-cabello, Pierre Boudry, Sabine RousselAbstract:Abalone growth rate is often identified among important traits to improve through selective breeding. However, the rapid success of some selective breeding plans has sometimes led to negative effects in some aquaculture species due to trade-offs. One of them is the loss of homeostasis of selected animals which results in the inability to resist the stress experienced during the rearing process. In this context, this study aimed to analyze the phenotypic relationships between growth, and physiological and behavioural traits in Haliotis tuberculata under stressful conditions. Eleven traits related to growth, immunity, reproduction and behaviour were recorded under laboratory conditions. A total of 120 adults from wild or farm origin were first monitored during a 3-week stress period (high density and acute stress handling) during winter, followed by 6 months on-growing in sea-cages. Relationships between parameters were analyzed using a multi-factorial approach. Wild and farm stocks could only be discriminated on behaviour traits, confirming that the French Abalone industry is in the beginning of the domestication process. After 3 weeks of chronic stress, the righting latency of an Abalone was linked to better survival and faster growth. Abalones having the best growth after 6 months were characterized by higher activity during the previous winter period, whereas an early gonad maturation reduced the growth in summer. Our results provide a basis for the establishment of a multi-trait breeding program to improve the growth rate while controlling the evolution of physiological and behavioural traits.ăStatement of relevanceăThe relationships of behavioural and physiological variables with survival and weight gain after application of important stressors were studied in order to provide a better understanding of Haliotis tuberculata biology during early domestication stage. This paper will give information on new targets and tools for selective breeding.
Rouvay Roodtwilding - One of the best experts on this subject based on the ideXlab platform.
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transcriptome characterization of the south african Abalone haliotis midae using sequencing by synthesis
BMC Research Notes, 2011Co-Authors: Paolo Franchini, Mathilde Van Der Merwe, Rouvay RoodtwildingAbstract:Background: Worldwide, the genus Haliotis is represented by 56 extant species and several of these are commercially cultured. Among the six Abalone species found in South Africa, Haliotis midae is the only aquacultured species. Despite its economic importance, genomic sequence resources for H. midae, and for Abalone in general, are still scarce. Next generation sequencing technologies provide a fast and efficient tool to generate large sequence collections that can be used to characterize the transcriptome and identify expressed genes associated with economically important traits like growth and disease resistance. Results: More than 25 million short reads generated by the Illumina Genome Analyzer were de novo assembled in 22,761 contigs with an average size of 260 bp. With a stringent E-value threshold of 10 -10 , 3,841 contigs (16.8%) had a BLAST homologous match against the Genbank non-redundant (NR) protein database. Most of these sequences were annotated using the gene ontology (GO) and eukaryotic orthologous groups of proteins (KOG) databases and assigned to various functional categories. According to annotation results, many gene families involved in immune response were identified. Thousands of simple sequence repeats (SSR) and single nucleotide polymorphisms (SNP) were detected. Setting stringent parameters to ensure a high probability of amplification, 420 primer pairs in 181 contigs containing SSR loci were designed. Conclusion: This data represents the most comprehensive genomic resource for the South African Abalone H. midae to date. The amount of assembled sequences demonstrated the utility of the Illumina sequencing technology in the transcriptome characterization of a non-model species. It allowed the development of several markers and the identification of promising candidate genes for future studies on population and functional genomics in H. midae and in other Abalone species. Background Abalones (Haliotis spp., Haliotidae) are important fishery resources worldwide, with high commercial value. Because of their sedentary lifestyle along shallow rocky coastlines [1], these gastropods are vulnerable to capture. The subsequent over-exploitation resulted in a substantial decrease of wild populations. To compensate for the high demand of Abalone, farming by means of aquaculture has been developed for several Haliotis species in different regions of their distribution area. Currently, Abalone aquaculture is practiced on 12 species in 16 different countries [2].
T A Probyn - One of the best experts on this subject based on the ideXlab platform.
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Integrated seaweed cultivation on an Abalone farm in South Africa
Journal of Applied Phycology, 2008Co-Authors: Deborah Robertson-andersson, Max Troell, R J Anderson, John J Bolton, Christina Halling, Michelle Potgieter, Joakim P. Hansen, T A ProbynAbstract:Land-based Abalone aquaculture in South Africa, based on the local species Haliotis midae, started in the early 1990s and has grown rapidly in the last decade, with 13 commercial farms now producing over 850 t per annum. Over 6,000 t per annum of kelp Ecklonia maxima are now harvested for this purpose, and some kelp beds are reaching maximum sustainable limits. Research into seaweed aquaculture as feed (Ulva and some Gracilaria) for Abalone started in the late 1990s on the southeast coast (where there are no kelp beds) using Abalone waste water. A growing body of evidence suggests that a mixed diet of kelp plus other seaweeds can give growth rates at least as good as compound feed, and can improve Abalone quality and reduce parasite loads. A pilot scale Ulva lactuca and Abalone integrated recirculation unit using 25% recirculation was designed and built on the south west coast of South Africa using one 12,000-L Abalone tank containing 13,200 15 ± 2.5 g Abalone, connected to two 3,000-L seaweed tanks containing an initial starting biomass of 10 kg of seaweed, replicated 3 times. In an 18-month period, there were no significant differences in Abalone health or growth rates, sediment build up and composition, mobile macro fauna densities and species between the recirculation or the flow-through units. Transfer of oxygen generated by the seaweeds to the Abalone tanks was poor, resulting in the recirculated Abalone tanks having lower (33%) dissolved oxygen concentrations than a comparable flow-through Abalone unit. Seaweed nutrient content and specific growth rates in the units were comparable to seaweeds cultivated in fertilized effluent (SGR = 3.2 ± 3.4%.day−1; Yield = 0.2 ± 0.19 kg.m2.day−1). Indications were that at this low recirculation ratio the seaweeds in the units were nutrient limited and that there were no negative effects to the Abalone being cultivated in such a recirculation unit at this recirculation ratio.
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Abalone farming in south africa an overview with perspectives on kelp resources Abalone feed potential for on farm seaweed production and socio economic importance
Aquaculture, 2006Co-Authors: Max Troell, Deborah Robertsonandersson, R J Anderson, John J Bolton, Gavin W Maneveldt, Christina Halling, T A ProbynAbstract:Abstract The South African Abalone cultivation industry has developed rapidly and is now the largest producer outside Asia. With a rapid decline in wild Abalone fisheries, farming now dominates the Abalone export market in South Africa. Kelp (Ecklonia maxima) constitutes the major feed for farmed Abalone in South Africa, but this resource is now approaching limits of sustainable harvesting in kelp Concession Areas where Abalone farms are concentrated. This paper gives an overview of the development of the South African Abalone industry and analyses how Abalone farming, natural kelp beds and seaweed harvesting are interlinked. It discusses options and constraints for expanding the Abalone industry, focussing especially on Abalone feed development to meet this growing demand. Kelp will continue to play an important role as feed and kelp areas previously not utilised may become cost-effective to harvest. There are many benefits from on-farm seaweed production and it will probably be a part of future expansion of the Abalone industry. Abalone waste discharges are not at present regarded as a major concern and farming brings important employment opportunities to lower income groups in remote coastal communities and has positive spillover effects on the seaweed industry and Abalone processing industry.
