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Abalone

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Sylvain Huchette – 1st expert on this subject based on the ideXlab platform

  • protective efficacy of a pseudoalteromonas strain in european Abalone haliotis tuberculata infected with vibrio harveyi orm4
    Probiotics and Antimicrobial Proteins, 2019
    Co-Authors: Clement Offret, Sylvain Huchette, Vincent Rochard, Helene Laguerre, Jerome Mounier, Benjamin Brillet, Patrick Le Chevalier, Yannick Fleury

    Abstract:

    : The hemolymph of healthy marine invertebrates is known to harbor antibiotic-producing bacteria belonging to the genus Pseudoalteromonas. Such strains are potential probiotics to control infectious diseases in aquaculture. In the present study, we screened a collection of Pseudoalteromonas strains isolated from the hemolymph of oyster and mussel for antimicrobial activity against Vibrio harveyi, a pathogenic species responsible for high mortality in Abalone. Subsequently, the protective efficacy of the most active strain named hCg-6 was investigated in Abalone culture faced with a Vibrio harveyi ORM4 infection. First, we have controlled the Pseudoalteromonas hCg-6 safety for Abalone health. To that end, animals were immersed for 4 h in Pseudoalteromonas hCg-6 suspensions in seawater. The Abalone viability was monitored and Pseudoalteromonas hCg-6 was tracked by quantitative-PCR in Abalone hemolymph. After immersion, no Abalone death occurred while the strain hCg-6 was significantly detected in hemolymph. Therefore, the strain hCg-6 was considered safe for Abalone and evaluated for its ability to protect Abalone against V. harveyi (injection of 1 × 103Vibrio per animal). A 4-h long immersion of Abalone in a seawater suspension of Pseudoalteromonas hCg-6 (1 × 106 CFU mL-1) prior to infection with Vibrio harveyi significantly improved the Abalone viability. Indeed, 15 days post infection, the hCg-6 treatment used increased the Abalone survival rate from 16% in untreated animals to 40% in treated Abalone. We hypothesized that Pseudoalteromonas hCg-6 antibacterial activity increased the hemomicrobiota shielding effect. In conclusion, Pseudoalteromonas hCg-6 is a promising anti-Vibrio strain for Abalone culture.

  • relationships between growth survival physiology and behaviour a multi criteria approach to haliotis tuberculata phenotypic traits
    Aquaculture, 2017
    Co-Authors: Sebastien Lachambre, Sylvain Huchette, Pierre Boudry, Antoine Riocabello, Timothee Fustec, Sabine Roussel

    Abstract:

    Abalone growth rate is often identified among important traits to improve through selective breeding. However, the rapid success of some selective breeding plans has sometimes led to negative effects in some aquaculture species due to trade-offs. One of them is the loss of homeostasis of selected animals which results in the inability to resist the stress experienced during the rearing process. In this context, this study aimed to analyze the phenotypic relationships between growth, and physiological and behavioural traits in Haliotis tuberculata under stressful conditions. Eleven traits related to growth, immunity, reproduction and behaviour were recorded under laboratory conditions. A total of 120 adults from wild or farm origin were first monitored during a 3-week stress period (high density and acute stress handling) during winter, followed by 6 months on-growing in sea-cages. Relationships between parameters were analyzed using a multi-factorial approach. Wild and farm stocks could only be discriminated on behaviour traits, confirming that the French Abalone industry is in the beginning of the domestication process. After 3 weeks of chronic stress, the righting latency of an Abalone was linked to better survival and faster growth. Abalones having the best growth after 6 months were characterized by higher activity during the previous winter period, whereas an early gonad maturation reduced the growth in summer. Our results provide a basis for the establishment of a multi-trait breeding program to improve the growth rate while controlling the evolution of physiological and behavioural traits.ăStatement of relevanceăThe relationships of behavioural and physiological variables with survival and weight gain after application of important stressors were studied in order to provide a better understanding of Haliotis tuberculata biology during early domestication stage. This paper will give information on new targets and tools for selective breeding.

  • Relationships between growth, survival, physiology and behaviour — A multi-criteria approach to Haliotis tuberculata phenotypic traits
    Aquaculture, 2017
    Co-Authors: Sebastien Lachambre, Sylvain Huchette, Pierre Boudry, Timothee Fustec, Antoine Rio-cabello, Sabine Roussel

    Abstract:

    Abalone growth rate is often identified among important traits to improve through selective breeding. However, the rapid success of some selective breeding plans has sometimes led to negative effects in some aquaculture species due to trade-offs. One of them is the loss of homeostasis of selected animals which results in the inability to resist the stress experienced during the rearing process. In this context, this study aimed to analyze the phenotypic relationships between growth, and physiological and behavioural traits in Haliotis tuberculata under stressful conditions. Eleven traits related to growth, immunity, reproduction and behaviour were recorded under laboratory conditions. A total of 120 adults from wild or farm origin were first monitored during a 3-week stress period (high density and acute stress handling) during winter, followed by 6 months on-growing in sea-cages. Relationships between parameters were analyzed using a multi-factorial approach. Wild and farm stocks could only be discriminated on behaviour traits, confirming that the French Abalone industry is in the beginning of the domestication process. After 3 weeks of chronic stress, the righting latency of an Abalone was linked to better survival and faster growth. Abalones having the best growth after 6 months were characterized by higher activity during the previous winter period, whereas an early gonad maturation reduced the growth in summer. Our results provide a basis for the establishment of a multi-trait breeding program to improve the growth rate while controlling the evolution of physiological and behavioural traits.ăStatement of relevanceăThe relationships of behavioural and physiological variables with survival and weight gain after application of important stressors were studied in order to provide a better understanding of Haliotis tuberculata biology during early domestication stage. This paper will give information on new targets and tools for selective breeding.

Christine Paillard – 2nd expert on this subject based on the ideXlab platform

  • vibrio harveyi adheres to and penetrates tissues of the european Abalone haliotis tuberculata within the first hours of contact
    Applied and Environmental Microbiology, 2014
    Co-Authors: Marion Cardinaud, Annaick Barbou, Carole Capitaine, Adeline Bidault, Antoine M Dujon, Dario Moraga, Christine Paillard

    Abstract:

    Vibrio harveyi is a marine bacterial pathogen responsible for episodic epidemics generally associated with massive mortalities in many marine organisms, including the European Abalone Haliotis tuberculata. The aim of this study was to identify the portal of entry and the dynamics of infection of V. harveyi in the European Abalone. The results indicate that the duration of contact be-tween V. harveyi and the European Abalone influences the mortality rate and precocity. Immediately after contact, the epithelial and mucosal area situated between the gills and the hypobranchial gland was colonized by V. harveyi. Real-time PCR analyses and culture quantification of a green fluorescent protein-tagged strain of V. harveyi in Abalone tissues revealed a high density of bacteria adhering to and then penetrating the whole gill-hypobranchial gland tissue after 1 h of contact. V. harveyi was also de-tected in the hemolymph of a significant number of European Abalones after 3 h of contact. In conclusion, this article shows that a TaqMan real-time PCR assay is a powerful and useful technique for the detection of a marine pathogen such as V. harveyi in mollusk tissue and for the study of its infection dynamics. Thus, we have revealed that the adhesion and then the penetration of V. harveyi in European Abalone organs begin in the first hours of contact. We also hypothesize that the portal of entry of V. har-veyi in the European Abalone is the area situated between the gills and the hypobranchial gland.

  • Characterization of Abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures
    Cytotechnology, 2013
    Co-Authors: Delphine Pichon, Benoit Cudennec, Sylvain Huchette, Chakib Djediat, Tristan Renault, Christine Paillard, Stéphanie Auzoux-bordenave

    Abstract:

    The decline of European Abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio . Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund Abalones, allowing in vivo and in vitro studies on the interactions between Abalone H. tuberculata and V. harveyi . This work reports the development of primary cell cultures from Abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell— V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days. Cytological parameters, cell morphology and viability were monitored over time using flow cytometry analysis and semi-quantitative assay (XTT). Then, gill cell cultures were used to investigate in vitro the interactions with V. harveyi . The effects of two bacterial strains were evaluated on gill cells: a pathogenic bacterial strain ORM4 which is responsible for Abalone mortalities and LMG7890 which is a non-pathogenic strain. Cellular responses of gill cells exposed to increasing concentrations of bacteria were evaluated by measuring mitochondrial activity (XTT assay) and phenoloxidase activity, an enzyme which is strongly involved in immune response. The ability of gill cells to phagocyte GFP-tagged V. harveyi was evaluated by flow cytometry and gill cells- V. harveyi interactions were characterized using fluorescence microscopy and transmission electron microscopy. During phagocytosis process we evidenced that V. harveyi bacteria induced significant changes in gill cells metabolism and immune response. Together, the results showed that primary cell cultures from Abalone gills are suitable for in vitro study of host-pathogen interactions, providing complementary assays to in vivo experiments.

  • Characterization of Abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures
    Cytotechnology, 2013
    Co-Authors: Delphine Pichon, Benoit Cudennec, Sylvain Huchette, Chakib Djediat, Tristan Renault, Christine Paillard, Stéphanie Auzoux-bordenave

    Abstract:

    The decline of European Abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund Abalones, allowing in vivo and in vitro studies on the interactions between Abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from Abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell-V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days. Cytological parameters, cell morphology and viability were monitored over time using flow cytometry analysis and semi-quantitative assay (XTT). Then, gill cell cultures were used to investigate in vitro the interactions with V. harveyi. The effects of two bacterial strains were evaluated on gill cells: a pathogenic bacterial strain ORM4 which is responsible for Abalone mortalities and LMG7890 which is a non-pathogenic strain. Cellular responses of gill cells exposed to increasing concentrations of bacteria were evaluated by measuring mitochondrial activity (XTT assay) and phenoloxidase activity, an enzyme which is strongly involved in immune response. The ability of gill cells to phagocyte GFP-tagged V. harveyi was evaluated by flow cytometry and gill cells-V. harveyi interactions were characterized using fluorescence microscopy and transmission electron microscopy. During phagocytosis process we evidenced that V. harveyi bacteria induced significant changes in gill cells metabolism and immune response. Together, the results showed that primary cell cultures from Abalone gills are suitable for in vitro study of host-pathogen interactions, providing complementary assays to in vivo experiments.

Sabine Roussel – 3rd expert on this subject based on the ideXlab platform

  • relationships between growth survival physiology and behaviour a multi criteria approach to haliotis tuberculata phenotypic traits
    Aquaculture, 2017
    Co-Authors: Sebastien Lachambre, Sylvain Huchette, Pierre Boudry, Antoine Riocabello, Timothee Fustec, Sabine Roussel

    Abstract:

    Abalone growth rate is often identified among important traits to improve through selective breeding. However, the rapid success of some selective breeding plans has sometimes led to negative effects in some aquaculture species due to trade-offs. One of them is the loss of homeostasis of selected animals which results in the inability to resist the stress experienced during the rearing process. In this context, this study aimed to analyze the phenotypic relationships between growth, and physiological and behavioural traits in Haliotis tuberculata under stressful conditions. Eleven traits related to growth, immunity, reproduction and behaviour were recorded under laboratory conditions. A total of 120 adults from wild or farm origin were first monitored during a 3-week stress period (high density and acute stress handling) during winter, followed by 6 months on-growing in sea-cages. Relationships between parameters were analyzed using a multi-factorial approach. Wild and farm stocks could only be discriminated on behaviour traits, confirming that the French Abalone industry is in the beginning of the domestication process. After 3 weeks of chronic stress, the righting latency of an Abalone was linked to better survival and faster growth. Abalones having the best growth after 6 months were characterized by higher activity during the previous winter period, whereas an early gonad maturation reduced the growth in summer. Our results provide a basis for the establishment of a multi-trait breeding program to improve the growth rate while controlling the evolution of physiological and behavioural traits.ăStatement of relevanceăThe relationships of behavioural and physiological variables with survival and weight gain after application of important stressors were studied in order to provide a better understanding of Haliotis tuberculata biology during early domestication stage. This paper will give information on new targets and tools for selective breeding.

  • Relationships between growth, survival, physiology and behaviour — A multi-criteria approach to Haliotis tuberculata phenotypic traits
    Aquaculture, 2017
    Co-Authors: Sebastien Lachambre, Sylvain Huchette, Pierre Boudry, Timothee Fustec, Antoine Rio-cabello, Sabine Roussel

    Abstract:

    Abalone growth rate is often identified among important traits to improve through selective breeding. However, the rapid success of some selective breeding plans has sometimes led to negative effects in some aquaculture species due to trade-offs. One of them is the loss of homeostasis of selected animals which results in the inability to resist the stress experienced during the rearing process. In this context, this study aimed to analyze the phenotypic relationships between growth, and physiological and behavioural traits in Haliotis tuberculata under stressful conditions. Eleven traits related to growth, immunity, reproduction and behaviour were recorded under laboratory conditions. A total of 120 adults from wild or farm origin were first monitored during a 3-week stress period (high density and acute stress handling) during winter, followed by 6 months on-growing in sea-cages. Relationships between parameters were analyzed using a multi-factorial approach. Wild and farm stocks could only be discriminated on behaviour traits, confirming that the French Abalone industry is in the beginning of the domestication process. After 3 weeks of chronic stress, the righting latency of an Abalone was linked to better survival and faster growth. Abalones having the best growth after 6 months were characterized by higher activity during the previous winter period, whereas an early gonad maturation reduced the growth in summer. Our results provide a basis for the establishment of a multi-trait breeding program to improve the growth rate while controlling the evolution of physiological and behavioural traits.ăStatement of relevanceăThe relationships of behavioural and physiological variables with survival and weight gain after application of important stressors were studied in order to provide a better understanding of Haliotis tuberculata biology during early domestication stage. This paper will give information on new targets and tools for selective breeding.