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Yuriy A Knirel – One of the best experts on this subject based on the ideXlab platform.

  • structure and gene cluster of the o antigen of escherichia coli o156 containing a pyruvic acid acetal
    Carbohydrate Research, 2016
    Co-Authors: Zhifeng Duan, Andrei V Perepelov, Sofya N Senchenkova, Alexander S Shashkov, Yuriy A Knirel
    Abstract:

    Abstract The lipopolysaccharide of Escherichia coli O156 was degraded under mild acidic and alkaline conditions and the resulting polysaccharides were studied by sugar analysis and 1H and 13C NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: Download : Download high-res image (31KB) Download : Download full-size image where Rpyr indicates R-configurated pyruvic acid acetal. Minor O-Acetyl groups also were present and tentatively localized on the Gal residues. The gene cluster for biosynthesis of the O-antigen of E. coli O156 was analyzed and shown to be consistent with the O-polysaccharide structure.

  • the o polysaccharide of escherichia coli o112ac has the same structure as that of shigella dysenteriae type 2 but is devoid of o Acetylation a revision of the s dysenteriae type 2 o polysaccharide structure
    Carbohydrate Research, 2008
    Co-Authors: Andrei V Perepelov, Andrej Weintraub, Sofya N Senchenkova, Alexander S Shashkov, Lu Feng, Lei Wang, Goran Widmalm, Yuriy A Knirel
    Abstract:

    Abstract The O-antigen structure of Shigella dysenteriae type 2 was reinvestigated using chemical modifications along with high-resolution 2D 1 H and 13 C NMR spectroscopy. The O-antigen was found to contain a pyruvic acid acetal, which was overlooked in an early study, and the following revised structure of the pentasaccharide repeating unit was established: Download full-size image where ∼70% GlcNAc residues bear an O –Acetyl group at position 3. The O-antigen of Escherichia coli O112ac was found to have the same carbohydrate structure but to lack O-Acetylation.

Andrei V Perepelov – One of the best experts on this subject based on the ideXlab platform.

  • structure and gene cluster of the o antigen of escherichia coli o156 containing a pyruvic acid acetal
    Carbohydrate Research, 2016
    Co-Authors: Zhifeng Duan, Andrei V Perepelov, Sofya N Senchenkova, Alexander S Shashkov, Yuriy A Knirel
    Abstract:

    Abstract The lipopolysaccharide of Escherichia coli O156 was degraded under mild acidic and alkaline conditions and the resulting polysaccharides were studied by sugar analysis and 1H and 13C NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: Download : Download high-res image (31KB) Download : Download full-size image where Rpyr indicates R-configurated pyruvic acid acetal. Minor O-Acetyl groups also were present and tentatively localized on the Gal residues. The gene cluster for biosynthesis of the O-antigen of E. coli O156 was analyzed and shown to be consistent with the O-polysaccharide structure.

  • the o polysaccharide of escherichia coli o112ac has the same structure as that of shigella dysenteriae type 2 but is devoid of o Acetylation a revision of the s dysenteriae type 2 o polysaccharide structure
    Carbohydrate Research, 2008
    Co-Authors: Andrei V Perepelov, Andrej Weintraub, Sofya N Senchenkova, Alexander S Shashkov, Lu Feng, Lei Wang, Goran Widmalm, Yuriy A Knirel
    Abstract:

    Abstract The O-antigen structure of Shigella dysenteriae type 2 was reinvestigated using chemical modifications along with high-resolution 2D 1 H and 13 C NMR spectroscopy. The O-antigen was found to contain a pyruvic acid acetal, which was overlooked in an early study, and the following revised structure of the pentasaccharide repeating unit was established: Download full-size image where ∼70% GlcNAc residues bear an O –Acetyl group at position 3. The O-antigen of Escherichia coli O112ac was found to have the same carbohydrate structure but to lack O-Acetylation.

Alexander S Shashkov – One of the best experts on this subject based on the ideXlab platform.

  • structure and gene cluster of the o antigen of escherichia coli o156 containing a pyruvic acid acetal
    Carbohydrate Research, 2016
    Co-Authors: Zhifeng Duan, Andrei V Perepelov, Sofya N Senchenkova, Alexander S Shashkov, Yuriy A Knirel
    Abstract:

    Abstract The lipopolysaccharide of Escherichia coli O156 was degraded under mild acidic and alkaline conditions and the resulting polysaccharides were studied by sugar analysis and 1H and 13C NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: Download : Download high-res image (31KB) Download : Download full-size image where Rpyr indicates R-configurated pyruvic acid acetal. Minor O-Acetyl groups also were present and tentatively localized on the Gal residues. The gene cluster for biosynthesis of the O-antigen of E. coli O156 was analyzed and shown to be consistent with the O-polysaccharide structure.

  • the o polysaccharide of escherichia coli o112ac has the same structure as that of shigella dysenteriae type 2 but is devoid of o Acetylation a revision of the s dysenteriae type 2 o polysaccharide structure
    Carbohydrate Research, 2008
    Co-Authors: Andrei V Perepelov, Andrej Weintraub, Sofya N Senchenkova, Alexander S Shashkov, Lu Feng, Lei Wang, Goran Widmalm, Yuriy A Knirel
    Abstract:

    Abstract The O-antigen structure of Shigella dysenteriae type 2 was reinvestigated using chemical modifications along with high-resolution 2D 1 H and 13 C NMR spectroscopy. The O-antigen was found to contain a pyruvic acid acetal, which was overlooked in an early study, and the following revised structure of the pentasaccharide repeating unit was established: Download full-size image where ∼70% GlcNAc residues bear an O –Acetyl group at position 3. The O-antigen of Escherichia coli O112ac was found to have the same carbohydrate structure but to lack O-Acetylation.