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Acid Hydrolase

The Experts below are selected from a list of 108 Experts worldwide ranked by ideXlab platform

Lawrence P. Wackett – 1st expert on this subject based on the ideXlab platform

  • Structure of the Cyanuric Acid Hydrolase TrzD Reveals Product Exit Channel
    Scientific Reports, 2017
    Co-Authors: Asim K. Bera, Kelly G. Aukema, Mikael Elias, Lawrence P. Wackett


    Cyanuric Acid Hydrolases are of industrial importance because of their use in aquatic recreational facilities to remove cyanuric Acid, a stabilizer for the chlorine. Degradation of excess cyanuric Acid is necessary to maintain chlorine disinfection in the waters. Cyanuric Acid Hydrolase opens the cyanuric Acid ring hydrolytically and subsequent decarboxylation produces carbon dioxide and biuret. In the present study, we report the X-ray structure of TrzD, a cyanuric Acid Hydrolase from Acidovorax citrulli. The crystal structure at 2.19 A resolution shows a large displacement of the catalytic lysine (Lys163) in domain 2 away from the active site core, whereas the two other active site lysines from the two other domains are not able to move. The lysine displacement is proposed here to open up a channel for product release. Consistent with that, the structure also showed two molecules of the co-product, carbon dioxide, one in the active site and another trapped in the proposed exit channel. Previous data indicated that the domain 2 lysine residue plays a role in activating an adjacent serine residue carrying out nucleophilic attack, opening the cyanuric Acid ring, and the mobile lysine guides products through the exit channel.

  • Bacterial Cyanuric Acid Hydrolase for Water Treatment
    Applied and Environmental Microbiology, 2015
    Co-Authors: Sujin Yeom, Baris R. Mutlu, Alptekin Aksan, Lawrence P. Wackett


    ABSTRACT Di- and trichloroisocyanuric Acids are widely used as water disinfection agents, but cyanuric Acid accumulates with repeated additions and must be removed to maintain free hypochlorite for disinfection. This study describes the development of methods for using a cyanuric Acid-degrading enzyme contained within nonliving cells that were encapsulated within a porous silica matrix. Initially, three different bacterial cyanuric Acid Hydrolases were compared: TrzD from Acidovorax citrulli strain 12227, AtzD from Pseudomonas sp. strain ADP, and CAH from Moorella thermoacetica ATCC 39073. Each enzyme was expressed recombinantly in Escherichia coli and tested for cyanuric Acid Hydrolase activity using freely suspended or encapsulated cell formats. Cyanuric Acid Hydrolase activities differed by only a 2-fold range when comparing across the different enzymes with a given format. A practical water filtration system is most likely to be used with nonviable cells, and all cells were rendered nonviable by heat treatment at 70°C for 1 h. Only the CAH enzyme from the thermophile M. thermoacetica retained significant activity under those conditions, and so it was tested in a flowthrough system simulating a bioreactive pool filter. Starting with a cyanuric Acid concentration of 10,000 μM, more than 70% of the cyanuric Acid was degraded in 24 h, it was completely removed in 72 h, and a respike of 10,000 μM cyanuric Acid a week later showed identical biodegradation kinetics. An experiment conducted with water obtained from municipal swimming pools showed the efficacy of the process, although cyanuric Acid degradation rates decreased by 50% in the presence of 4.5 ppm hypochlorite. In total, these experiments demonstrated significant robustness of cyanuric Acid Hydrolase and the silica bead materials in remediation.

  • Expanding the Cyanuric Acid Hydrolase Protein Family to the Fungal Kingdom
    Journal of Bacteriology, 2013
    Co-Authors: Anthony G. Dodge, Chelsea S. Preiner, Lawrence P. Wackett


    The known enzymes that open the s-triazine ring, the cyanuric Acid Hydrolases, have been confined almost exclusively to the kingdom Bacteria and are all homologous members of the rare cyanuric Acid Hydrolase/barbiturase protein family. In the present study, a filamentous fungus, Sarocladium sp. strain CA, was isolated from soil by enrichment culturing using cyanuric Acid as the sole source of nitrogen. A reverse-genetic approach identified a fungal cyanuric Acid Hydrolase gene composed of two exons and one intron. The translated spliced sequence was 39 to 53% identical to previously characterized bacterial cyanuric Acid Hydrolases. The sequence was used to generate a gene optimized for expression in Escherichia coli and encoding an N-terminally histidine-tagged protein. The protein was purified by nickel affinity and anion-exchange chromatography. The purified protein was shown by 13C nuclear magnetic resonance (13C-NMR) to produce carboxybiuret as the product, which spontaneously decarboxylated to yield biuret and carbon dioxide. The protein was very narrow in substrate specificity, showing activity only with cyanuric Acid and N-methyl cyanuric Acid. Barbituric Acid was an inhibitor of enzyme activity. Sequence analysis identified genes with introns in other fungi from the Ascomycota that, if spliced, are predicted to encode proteins with cyanuric Acid Hydrolase activity. The Ascomycota cyanuric Acid Hydrolase homologs are most closely related to cyanuric Acid Hydrolases from Actinobacteria.

Juan S Bonifacino – 2nd expert on this subject based on the ideXlab platform

  • requirement of the human garp complex for mannose 6 phosphate receptor dependent sorting of cathepsin d to lysosomes
    Molecular Biology of the Cell, 2008
    Co-Authors: Javier F Perezvictoria, Gonzalo A Mardones, Juan S Bonifacino


    The biosynthetic sorting of Acid Hydrolases to lysosomes relies on transmembrane, mannose 6-phosphate receptors (MPRs) that cycle between the TGN and endosomes. Herein we report that maintenance of this cycling requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex. Depletion of any of the three GARP subunits, Vps52, Vps53, or Vps54, by RNAi impairs sorting of the precursor of the Acid Hydrolase, cathepsin D, to lysosomes and leads to its secretion into the culture medium. As a consequence, lysosomes become swollen, likely due to a buildup of undegraded materials. Missorting of cathepsin D in GARP-depleted cells results from accumulation of recycling MPRs in a population of light, small vesicles downstream of endosomes. These vesicles might correspond to intermediates in retrograde transport from endosomes to the TGN. Depletion of GARP subunits also blocks the retrograde transport of the TGN protein, TGN46, and the B subunit of Shiga toxin. These observations indicate that the mammalian GARP complex plays a general role in the delivery of retrograde cargo into the TGN. We also report that a Vps54 mutant protein in the Wobbler mouse strain is active in retrograde transport, thus explaining the viability of these mutant mice.

Bruce W. Trotman – 3rd expert on this subject based on the ideXlab platform

  • Pigment gallstone disease.
    Gastroenterology clinics of North America, 1991
    Co-Authors: Bruce W. Trotman


    Black and brown pigment gallstones are morphologically, compositionally, and clinically distinct. Black stones form primarily in the gallbladder in sterile bile and are associated with advanced age, chronic hemolysis, alcoholism, cirrhosis, pancreatitis, and total parenteral nutrition. Brown stones form not only within the gallbladder but also within the intrahepatic and extrahepatic ducts; they are uniformly infected with enteric bacteria and are usually associated with ascending cholangitis. Brown stones are related to juxtapapillary duodenal diverticula and are the predominant type of de novo common bile duct stones. Cholecystectomy is usually curative in black pigment stone disease, whereas stones often recur after cholecystectomy for brown stone disease. The pathogenesis of black stones is probably related to nonbacterial, nonenzymatic hydrolysis of bilirubin conjugates. At the pH of bile, this results in two monohydrogenated bilirubin anions that precipitate with calcium ions. Bilirubin monoconjugates that are increased in several conditions, such as Gilbert’s syndrome and chronic hemolysis, may play a pivotal role in black stone formation as a source of unconjugated monohydrogenated bilirubin and as a possible co-precipitant with calcium. The precipitation of calcium carbonate and phosphate is influenced by local gallbladder factors. Brown pigment stones are formed in bile infected with enteric bacteria that elaborate hydrolytic enzymes: beta-glucuronidase, phospholipase A, and conjugated bile Acid Hydrolase. The resulting anions of bilirubin and fatty Acids form insoluble calcium salts. We used nb/nb mice with a chronic hemolytic anemia as a model of hemolysis-induced black stone disease. The presence of 40% bilirubin monoconjugates in mouse gallstones indicated the importance of this moiety in the pathogenesis of black stones. Other data obtained by marrow transplantation experiments in mice revealed the relative importance of genotype versus the hemolytic anemia on determinants such as biliary bile Acid composition and mucin secretory glands in the mouse gallbladder neck. Additional physical chemical studies of the interaction of unconjugated bilirubin in model bile solutions will be helpful in further delineating the pathogenesis of both black and brown pigment gallstones.