The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Athanasios A. Koutinas - One of the best experts on this subject based on the ideXlab platform.
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new generation biofuel from whey successive Acidogenesis and alcoholic fermentation using immobilized cultures on γ alumina
Energy Conversion and Management, 2017Co-Authors: Konstantina Boura, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Dionysios Kolliopoulos, Dimitrios Vasileiou, Panayiotis Panas, Athanasios A. KoutinasAbstract:Abstract Cheese whey exploitation in a biorefinery manner is proposed involving anaerobic Acidogenesis by a UASB mixed anaerobic culture and alcoholic fermentation by kefir. Both cultures were immobilized on γ-alumina. The produced organic acids (OAs) and ethanol could be esterified to obtain a novel ester-based biofuel similar to biodiesel. During Acidogenesis, lactic acid-type fermentation occurred leading to 12 g L −1 total OAs and 0.2 g L −1 ethanol. The fermented substrate was subsequently supplied to a second bioreactor with immobilized kefir, which increased the OAs content (15 g L −1 ), especially lactic acid, and slightly the ethanol concentration (0.3–0.4 g L −1 ). To further increase ethanol concentration, a second experiment was conducted supplying whey firstly to the immobilized kefir bioreactor and then pumping the effluent into the Acidogenesis bioreactor, resulting in 40% increase of OAs and 10-fold higher ethanol content. The residual sugar was ∼50% of the initial whey lactose; consequently, future research could result to further increase of ethanol and OAs.
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Acidogenesis of cellulosic hydrolysates for new generation biofuels
Biomass & Bioenergy, 2016Co-Authors: Panagiotis Kandylis, Katerina Lappa, Argyro Bekatorou, Maria Kanellaki, Katerina Pissaridi, Agapi Dima, Athanasios A. KoutinasAbstract:Abstract This article presents a comprehensive review on hydrolysis and Acidogenesis of lignocellulosic waste biomass and makes clear new perspectives in biofuel research. Specifically, the Acidogenesis of lignocellulosics and liquid effluent have been discussed extensively with potential goal the production of a new generation biofuel. This new biofuel can be produced through esterification of volatile fatty acids with ethanol (produced simultaneously during the Acidogenesis) or/and with another alcohol. That will overcome the major problems faced during bioethanol production and concerns the high energy demand of the bioethanol production plant. Specifically, it was found that the main volatile fatty acids formed are formic, acetic, propionic, butyric, lactic and valeric. Their formation depends on NADH/NAD+ proportion and on conditions such as pH, organic load and chemical composition of the waste is treated. These conditions look to affect microorganisms survival and the formation of predominant acetic, butyric and lactic acid. The use of γ-alumina promotes the formation of volatile fatty acids simultaneously with bio-ethanol.
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continuous Acidogenesis of sucrose raffinose and vinasse using mineral kissiris as promoter
Bioresource Technology, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:Abstract The use of kissiris as promoter (culture immobilization carrier) in anaerobic Acidogenesis of sucrose, raffinose and vinasse is reported. Initially, the effect of pH (4–8) and fermentation temperature (18–52 °C) on the accumulation of low molecular weight organic acids (OAs) during sucrose Acidogenesis, was evaluated. The promoting effect of kissiris was confirmed compared to free cells, resulting in 80% increased OAs production. The optimum conditions (pH 8; 37 °C) were used during Acidogenesis of sucrose/raffinose mixtures. A continuous system was also operated for more than 2 months. When sucrose and sucrose/raffinose mixtures were used, lactic acid type fermentation prevailed, while when vinasse was used, butyric acid type fermentation occurred. Total OAs concentrations were more than 14 g/L and ethanol concentrations were 0.5–1 mL/L. Culture adaptation in vinasse was necessary to avoid poor results. The proposed process is promising for new generation ester-based biofuel production from industrial wastes.
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new generation biofuel continuous Acidogenesis of sucrose raffinose mixture simulating vinasse is promoted by γ alumina pellets
Biotechnology for Biofuels, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:Background This investigation comprises a contribution on the production of a new generation biofuel using the industrial liquid waste of bioethanol distilleries, known as vinasse. This study focuses on the exploitation of vinasse as an Acidogenesis substrate for volatile fatty acids and simultaneous ethanol production. These products can be used for ester production, which is the new generation biofuel. Therefore, the aims of the present study are (i) to examine any promotional effect of γ-alumina on Acidogenesis of a sucrose-raffinose mixture simulating vinasse, (ii) to study the operational stability of the continuous Acidogenesis of sucrose and raffinose and subsequently of vinasse, and (iii) to determine the volatile fatty acid chemical composition and ethanol formation.
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new generation biofuel continuous Acidogenesis of sucrose raffinose mixture simulating vinasse is promoted by γ alumina pellets
Biotechnology for Biofuels, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:This investigation comprises a contribution on the production of a new generation biofuel using the industrial liquid waste of bioethanol distilleries, known as vinasse. This study focuses on the exploitation of vinasse as an Acidogenesis substrate for volatile fatty acids and simultaneous ethanol production. These products can be used for ester production, which is the new generation biofuel. Therefore, the aims of the present study are (i) to examine any promotional effect of γ-alumina on Acidogenesis of a sucrose-raffinose mixture simulating vinasse, (ii) to study the operational stability of the continuous Acidogenesis of sucrose and raffinose and subsequently of vinasse, and (iii) to determine the volatile fatty acid chemical composition and ethanol formation. Batch Acidogenesis of sucrose and raffinose mixtures showed that γ-alumina promoted fermentation leading to an increase in the volatile fatty acid yield factor from 40% to 95% compared to free cells. The application of the system in continuous mode for more than 3 months showed that the continuous volatile fatty acid productivity obtained was higher than 7 g/L/day. Lactic acid was the predominant acid when sucrose and raffinose were used while butyric acid in the case of vinasse. The highest volatile fatty acid concentration reached was 19 g/L for vinasse. A promoting effect of γ-alumina in acidogenic fermentation of sucrose-raffinose and vinasse is reported. Continuous Acidogenesis of sucrose-raffinose mixtures and vinasse using γ-alumina with immobilized cells showed high operational stability (more than 3 months). These findings result in easy scale up of the process that will produce a high annual added value of $11,000,000 in a daily bioethanol production plant of 50,000 L.
Katerina Lappa - One of the best experts on this subject based on the ideXlab platform.
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Acidogenesis of cellulosic hydrolysates for new generation biofuels
Biomass & Bioenergy, 2016Co-Authors: Panagiotis Kandylis, Katerina Lappa, Argyro Bekatorou, Maria Kanellaki, Katerina Pissaridi, Agapi Dima, Athanasios A. KoutinasAbstract:Abstract This article presents a comprehensive review on hydrolysis and Acidogenesis of lignocellulosic waste biomass and makes clear new perspectives in biofuel research. Specifically, the Acidogenesis of lignocellulosics and liquid effluent have been discussed extensively with potential goal the production of a new generation biofuel. This new biofuel can be produced through esterification of volatile fatty acids with ethanol (produced simultaneously during the Acidogenesis) or/and with another alcohol. That will overcome the major problems faced during bioethanol production and concerns the high energy demand of the bioethanol production plant. Specifically, it was found that the main volatile fatty acids formed are formic, acetic, propionic, butyric, lactic and valeric. Their formation depends on NADH/NAD+ proportion and on conditions such as pH, organic load and chemical composition of the waste is treated. These conditions look to affect microorganisms survival and the formation of predominant acetic, butyric and lactic acid. The use of γ-alumina promotes the formation of volatile fatty acids simultaneously with bio-ethanol.
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continuous Acidogenesis of sucrose raffinose and vinasse using mineral kissiris as promoter
Bioresource Technology, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:Abstract The use of kissiris as promoter (culture immobilization carrier) in anaerobic Acidogenesis of sucrose, raffinose and vinasse is reported. Initially, the effect of pH (4–8) and fermentation temperature (18–52 °C) on the accumulation of low molecular weight organic acids (OAs) during sucrose Acidogenesis, was evaluated. The promoting effect of kissiris was confirmed compared to free cells, resulting in 80% increased OAs production. The optimum conditions (pH 8; 37 °C) were used during Acidogenesis of sucrose/raffinose mixtures. A continuous system was also operated for more than 2 months. When sucrose and sucrose/raffinose mixtures were used, lactic acid type fermentation prevailed, while when vinasse was used, butyric acid type fermentation occurred. Total OAs concentrations were more than 14 g/L and ethanol concentrations were 0.5–1 mL/L. Culture adaptation in vinasse was necessary to avoid poor results. The proposed process is promising for new generation ester-based biofuel production from industrial wastes.
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new generation biofuel continuous Acidogenesis of sucrose raffinose mixture simulating vinasse is promoted by γ alumina pellets
Biotechnology for Biofuels, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:Background This investigation comprises a contribution on the production of a new generation biofuel using the industrial liquid waste of bioethanol distilleries, known as vinasse. This study focuses on the exploitation of vinasse as an Acidogenesis substrate for volatile fatty acids and simultaneous ethanol production. These products can be used for ester production, which is the new generation biofuel. Therefore, the aims of the present study are (i) to examine any promotional effect of γ-alumina on Acidogenesis of a sucrose-raffinose mixture simulating vinasse, (ii) to study the operational stability of the continuous Acidogenesis of sucrose and raffinose and subsequently of vinasse, and (iii) to determine the volatile fatty acid chemical composition and ethanol formation.
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new generation biofuel continuous Acidogenesis of sucrose raffinose mixture simulating vinasse is promoted by γ alumina pellets
Biotechnology for Biofuels, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:This investigation comprises a contribution on the production of a new generation biofuel using the industrial liquid waste of bioethanol distilleries, known as vinasse. This study focuses on the exploitation of vinasse as an Acidogenesis substrate for volatile fatty acids and simultaneous ethanol production. These products can be used for ester production, which is the new generation biofuel. Therefore, the aims of the present study are (i) to examine any promotional effect of γ-alumina on Acidogenesis of a sucrose-raffinose mixture simulating vinasse, (ii) to study the operational stability of the continuous Acidogenesis of sucrose and raffinose and subsequently of vinasse, and (iii) to determine the volatile fatty acid chemical composition and ethanol formation. Batch Acidogenesis of sucrose and raffinose mixtures showed that γ-alumina promoted fermentation leading to an increase in the volatile fatty acid yield factor from 40% to 95% compared to free cells. The application of the system in continuous mode for more than 3 months showed that the continuous volatile fatty acid productivity obtained was higher than 7 g/L/day. Lactic acid was the predominant acid when sucrose and raffinose were used while butyric acid in the case of vinasse. The highest volatile fatty acid concentration reached was 19 g/L for vinasse. A promoting effect of γ-alumina in acidogenic fermentation of sucrose-raffinose and vinasse is reported. Continuous Acidogenesis of sucrose-raffinose mixtures and vinasse using γ-alumina with immobilized cells showed high operational stability (more than 3 months). These findings result in easy scale up of the process that will produce a high annual added value of $11,000,000 in a daily bioethanol production plant of 50,000 L.
Panagiotis Kandylis - One of the best experts on this subject based on the ideXlab platform.
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new generation biofuel from whey successive Acidogenesis and alcoholic fermentation using immobilized cultures on γ alumina
Energy Conversion and Management, 2017Co-Authors: Konstantina Boura, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Dionysios Kolliopoulos, Dimitrios Vasileiou, Panayiotis Panas, Athanasios A. KoutinasAbstract:Abstract Cheese whey exploitation in a biorefinery manner is proposed involving anaerobic Acidogenesis by a UASB mixed anaerobic culture and alcoholic fermentation by kefir. Both cultures were immobilized on γ-alumina. The produced organic acids (OAs) and ethanol could be esterified to obtain a novel ester-based biofuel similar to biodiesel. During Acidogenesis, lactic acid-type fermentation occurred leading to 12 g L −1 total OAs and 0.2 g L −1 ethanol. The fermented substrate was subsequently supplied to a second bioreactor with immobilized kefir, which increased the OAs content (15 g L −1 ), especially lactic acid, and slightly the ethanol concentration (0.3–0.4 g L −1 ). To further increase ethanol concentration, a second experiment was conducted supplying whey firstly to the immobilized kefir bioreactor and then pumping the effluent into the Acidogenesis bioreactor, resulting in 40% increase of OAs and 10-fold higher ethanol content. The residual sugar was ∼50% of the initial whey lactose; consequently, future research could result to further increase of ethanol and OAs.
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Acidogenesis of cellulosic hydrolysates for new generation biofuels
Biomass & Bioenergy, 2016Co-Authors: Panagiotis Kandylis, Katerina Lappa, Argyro Bekatorou, Maria Kanellaki, Katerina Pissaridi, Agapi Dima, Athanasios A. KoutinasAbstract:Abstract This article presents a comprehensive review on hydrolysis and Acidogenesis of lignocellulosic waste biomass and makes clear new perspectives in biofuel research. Specifically, the Acidogenesis of lignocellulosics and liquid effluent have been discussed extensively with potential goal the production of a new generation biofuel. This new biofuel can be produced through esterification of volatile fatty acids with ethanol (produced simultaneously during the Acidogenesis) or/and with another alcohol. That will overcome the major problems faced during bioethanol production and concerns the high energy demand of the bioethanol production plant. Specifically, it was found that the main volatile fatty acids formed are formic, acetic, propionic, butyric, lactic and valeric. Their formation depends on NADH/NAD+ proportion and on conditions such as pH, organic load and chemical composition of the waste is treated. These conditions look to affect microorganisms survival and the formation of predominant acetic, butyric and lactic acid. The use of γ-alumina promotes the formation of volatile fatty acids simultaneously with bio-ethanol.
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continuous Acidogenesis of sucrose raffinose and vinasse using mineral kissiris as promoter
Bioresource Technology, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:Abstract The use of kissiris as promoter (culture immobilization carrier) in anaerobic Acidogenesis of sucrose, raffinose and vinasse is reported. Initially, the effect of pH (4–8) and fermentation temperature (18–52 °C) on the accumulation of low molecular weight organic acids (OAs) during sucrose Acidogenesis, was evaluated. The promoting effect of kissiris was confirmed compared to free cells, resulting in 80% increased OAs production. The optimum conditions (pH 8; 37 °C) were used during Acidogenesis of sucrose/raffinose mixtures. A continuous system was also operated for more than 2 months. When sucrose and sucrose/raffinose mixtures were used, lactic acid type fermentation prevailed, while when vinasse was used, butyric acid type fermentation occurred. Total OAs concentrations were more than 14 g/L and ethanol concentrations were 0.5–1 mL/L. Culture adaptation in vinasse was necessary to avoid poor results. The proposed process is promising for new generation ester-based biofuel production from industrial wastes.
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new generation biofuel continuous Acidogenesis of sucrose raffinose mixture simulating vinasse is promoted by γ alumina pellets
Biotechnology for Biofuels, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:Background This investigation comprises a contribution on the production of a new generation biofuel using the industrial liquid waste of bioethanol distilleries, known as vinasse. This study focuses on the exploitation of vinasse as an Acidogenesis substrate for volatile fatty acids and simultaneous ethanol production. These products can be used for ester production, which is the new generation biofuel. Therefore, the aims of the present study are (i) to examine any promotional effect of γ-alumina on Acidogenesis of a sucrose-raffinose mixture simulating vinasse, (ii) to study the operational stability of the continuous Acidogenesis of sucrose and raffinose and subsequently of vinasse, and (iii) to determine the volatile fatty acid chemical composition and ethanol formation.
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new generation biofuel continuous Acidogenesis of sucrose raffinose mixture simulating vinasse is promoted by γ alumina pellets
Biotechnology for Biofuels, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:This investigation comprises a contribution on the production of a new generation biofuel using the industrial liquid waste of bioethanol distilleries, known as vinasse. This study focuses on the exploitation of vinasse as an Acidogenesis substrate for volatile fatty acids and simultaneous ethanol production. These products can be used for ester production, which is the new generation biofuel. Therefore, the aims of the present study are (i) to examine any promotional effect of γ-alumina on Acidogenesis of a sucrose-raffinose mixture simulating vinasse, (ii) to study the operational stability of the continuous Acidogenesis of sucrose and raffinose and subsequently of vinasse, and (iii) to determine the volatile fatty acid chemical composition and ethanol formation. Batch Acidogenesis of sucrose and raffinose mixtures showed that γ-alumina promoted fermentation leading to an increase in the volatile fatty acid yield factor from 40% to 95% compared to free cells. The application of the system in continuous mode for more than 3 months showed that the continuous volatile fatty acid productivity obtained was higher than 7 g/L/day. Lactic acid was the predominant acid when sucrose and raffinose were used while butyric acid in the case of vinasse. The highest volatile fatty acid concentration reached was 19 g/L for vinasse. A promoting effect of γ-alumina in acidogenic fermentation of sucrose-raffinose and vinasse is reported. Continuous Acidogenesis of sucrose-raffinose mixtures and vinasse using γ-alumina with immobilized cells showed high operational stability (more than 3 months). These findings result in easy scale up of the process that will produce a high annual added value of $11,000,000 in a daily bioethanol production plant of 50,000 L.
Maria Kanellaki - One of the best experts on this subject based on the ideXlab platform.
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new generation biofuel from whey successive Acidogenesis and alcoholic fermentation using immobilized cultures on γ alumina
Energy Conversion and Management, 2017Co-Authors: Konstantina Boura, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Dionysios Kolliopoulos, Dimitrios Vasileiou, Panayiotis Panas, Athanasios A. KoutinasAbstract:Abstract Cheese whey exploitation in a biorefinery manner is proposed involving anaerobic Acidogenesis by a UASB mixed anaerobic culture and alcoholic fermentation by kefir. Both cultures were immobilized on γ-alumina. The produced organic acids (OAs) and ethanol could be esterified to obtain a novel ester-based biofuel similar to biodiesel. During Acidogenesis, lactic acid-type fermentation occurred leading to 12 g L −1 total OAs and 0.2 g L −1 ethanol. The fermented substrate was subsequently supplied to a second bioreactor with immobilized kefir, which increased the OAs content (15 g L −1 ), especially lactic acid, and slightly the ethanol concentration (0.3–0.4 g L −1 ). To further increase ethanol concentration, a second experiment was conducted supplying whey firstly to the immobilized kefir bioreactor and then pumping the effluent into the Acidogenesis bioreactor, resulting in 40% increase of OAs and 10-fold higher ethanol content. The residual sugar was ∼50% of the initial whey lactose; consequently, future research could result to further increase of ethanol and OAs.
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Acidogenesis of cellulosic hydrolysates for new generation biofuels
Biomass & Bioenergy, 2016Co-Authors: Panagiotis Kandylis, Katerina Lappa, Argyro Bekatorou, Maria Kanellaki, Katerina Pissaridi, Agapi Dima, Athanasios A. KoutinasAbstract:Abstract This article presents a comprehensive review on hydrolysis and Acidogenesis of lignocellulosic waste biomass and makes clear new perspectives in biofuel research. Specifically, the Acidogenesis of lignocellulosics and liquid effluent have been discussed extensively with potential goal the production of a new generation biofuel. This new biofuel can be produced through esterification of volatile fatty acids with ethanol (produced simultaneously during the Acidogenesis) or/and with another alcohol. That will overcome the major problems faced during bioethanol production and concerns the high energy demand of the bioethanol production plant. Specifically, it was found that the main volatile fatty acids formed are formic, acetic, propionic, butyric, lactic and valeric. Their formation depends on NADH/NAD+ proportion and on conditions such as pH, organic load and chemical composition of the waste is treated. These conditions look to affect microorganisms survival and the formation of predominant acetic, butyric and lactic acid. The use of γ-alumina promotes the formation of volatile fatty acids simultaneously with bio-ethanol.
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continuous Acidogenesis of sucrose raffinose and vinasse using mineral kissiris as promoter
Bioresource Technology, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:Abstract The use of kissiris as promoter (culture immobilization carrier) in anaerobic Acidogenesis of sucrose, raffinose and vinasse is reported. Initially, the effect of pH (4–8) and fermentation temperature (18–52 °C) on the accumulation of low molecular weight organic acids (OAs) during sucrose Acidogenesis, was evaluated. The promoting effect of kissiris was confirmed compared to free cells, resulting in 80% increased OAs production. The optimum conditions (pH 8; 37 °C) were used during Acidogenesis of sucrose/raffinose mixtures. A continuous system was also operated for more than 2 months. When sucrose and sucrose/raffinose mixtures were used, lactic acid type fermentation prevailed, while when vinasse was used, butyric acid type fermentation occurred. Total OAs concentrations were more than 14 g/L and ethanol concentrations were 0.5–1 mL/L. Culture adaptation in vinasse was necessary to avoid poor results. The proposed process is promising for new generation ester-based biofuel production from industrial wastes.
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new generation biofuel continuous Acidogenesis of sucrose raffinose mixture simulating vinasse is promoted by γ alumina pellets
Biotechnology for Biofuels, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:Background This investigation comprises a contribution on the production of a new generation biofuel using the industrial liquid waste of bioethanol distilleries, known as vinasse. This study focuses on the exploitation of vinasse as an Acidogenesis substrate for volatile fatty acids and simultaneous ethanol production. These products can be used for ester production, which is the new generation biofuel. Therefore, the aims of the present study are (i) to examine any promotional effect of γ-alumina on Acidogenesis of a sucrose-raffinose mixture simulating vinasse, (ii) to study the operational stability of the continuous Acidogenesis of sucrose and raffinose and subsequently of vinasse, and (iii) to determine the volatile fatty acid chemical composition and ethanol formation.
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new generation biofuel continuous Acidogenesis of sucrose raffinose mixture simulating vinasse is promoted by γ alumina pellets
Biotechnology for Biofuels, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:This investigation comprises a contribution on the production of a new generation biofuel using the industrial liquid waste of bioethanol distilleries, known as vinasse. This study focuses on the exploitation of vinasse as an Acidogenesis substrate for volatile fatty acids and simultaneous ethanol production. These products can be used for ester production, which is the new generation biofuel. Therefore, the aims of the present study are (i) to examine any promotional effect of γ-alumina on Acidogenesis of a sucrose-raffinose mixture simulating vinasse, (ii) to study the operational stability of the continuous Acidogenesis of sucrose and raffinose and subsequently of vinasse, and (iii) to determine the volatile fatty acid chemical composition and ethanol formation. Batch Acidogenesis of sucrose and raffinose mixtures showed that γ-alumina promoted fermentation leading to an increase in the volatile fatty acid yield factor from 40% to 95% compared to free cells. The application of the system in continuous mode for more than 3 months showed that the continuous volatile fatty acid productivity obtained was higher than 7 g/L/day. Lactic acid was the predominant acid when sucrose and raffinose were used while butyric acid in the case of vinasse. The highest volatile fatty acid concentration reached was 19 g/L for vinasse. A promoting effect of γ-alumina in acidogenic fermentation of sucrose-raffinose and vinasse is reported. Continuous Acidogenesis of sucrose-raffinose mixtures and vinasse using γ-alumina with immobilized cells showed high operational stability (more than 3 months). These findings result in easy scale up of the process that will produce a high annual added value of $11,000,000 in a daily bioethanol production plant of 50,000 L.
Argyro Bekatorou - One of the best experts on this subject based on the ideXlab platform.
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new generation biofuel from whey successive Acidogenesis and alcoholic fermentation using immobilized cultures on γ alumina
Energy Conversion and Management, 2017Co-Authors: Konstantina Boura, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Dionysios Kolliopoulos, Dimitrios Vasileiou, Panayiotis Panas, Athanasios A. KoutinasAbstract:Abstract Cheese whey exploitation in a biorefinery manner is proposed involving anaerobic Acidogenesis by a UASB mixed anaerobic culture and alcoholic fermentation by kefir. Both cultures were immobilized on γ-alumina. The produced organic acids (OAs) and ethanol could be esterified to obtain a novel ester-based biofuel similar to biodiesel. During Acidogenesis, lactic acid-type fermentation occurred leading to 12 g L −1 total OAs and 0.2 g L −1 ethanol. The fermented substrate was subsequently supplied to a second bioreactor with immobilized kefir, which increased the OAs content (15 g L −1 ), especially lactic acid, and slightly the ethanol concentration (0.3–0.4 g L −1 ). To further increase ethanol concentration, a second experiment was conducted supplying whey firstly to the immobilized kefir bioreactor and then pumping the effluent into the Acidogenesis bioreactor, resulting in 40% increase of OAs and 10-fold higher ethanol content. The residual sugar was ∼50% of the initial whey lactose; consequently, future research could result to further increase of ethanol and OAs.
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Acidogenesis of cellulosic hydrolysates for new generation biofuels
Biomass & Bioenergy, 2016Co-Authors: Panagiotis Kandylis, Katerina Lappa, Argyro Bekatorou, Maria Kanellaki, Katerina Pissaridi, Agapi Dima, Athanasios A. KoutinasAbstract:Abstract This article presents a comprehensive review on hydrolysis and Acidogenesis of lignocellulosic waste biomass and makes clear new perspectives in biofuel research. Specifically, the Acidogenesis of lignocellulosics and liquid effluent have been discussed extensively with potential goal the production of a new generation biofuel. This new biofuel can be produced through esterification of volatile fatty acids with ethanol (produced simultaneously during the Acidogenesis) or/and with another alcohol. That will overcome the major problems faced during bioethanol production and concerns the high energy demand of the bioethanol production plant. Specifically, it was found that the main volatile fatty acids formed are formic, acetic, propionic, butyric, lactic and valeric. Their formation depends on NADH/NAD+ proportion and on conditions such as pH, organic load and chemical composition of the waste is treated. These conditions look to affect microorganisms survival and the formation of predominant acetic, butyric and lactic acid. The use of γ-alumina promotes the formation of volatile fatty acids simultaneously with bio-ethanol.
-
continuous Acidogenesis of sucrose raffinose and vinasse using mineral kissiris as promoter
Bioresource Technology, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:Abstract The use of kissiris as promoter (culture immobilization carrier) in anaerobic Acidogenesis of sucrose, raffinose and vinasse is reported. Initially, the effect of pH (4–8) and fermentation temperature (18–52 °C) on the accumulation of low molecular weight organic acids (OAs) during sucrose Acidogenesis, was evaluated. The promoting effect of kissiris was confirmed compared to free cells, resulting in 80% increased OAs production. The optimum conditions (pH 8; 37 °C) were used during Acidogenesis of sucrose/raffinose mixtures. A continuous system was also operated for more than 2 months. When sucrose and sucrose/raffinose mixtures were used, lactic acid type fermentation prevailed, while when vinasse was used, butyric acid type fermentation occurred. Total OAs concentrations were more than 14 g/L and ethanol concentrations were 0.5–1 mL/L. Culture adaptation in vinasse was necessary to avoid poor results. The proposed process is promising for new generation ester-based biofuel production from industrial wastes.
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new generation biofuel continuous Acidogenesis of sucrose raffinose mixture simulating vinasse is promoted by γ alumina pellets
Biotechnology for Biofuels, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:Background This investigation comprises a contribution on the production of a new generation biofuel using the industrial liquid waste of bioethanol distilleries, known as vinasse. This study focuses on the exploitation of vinasse as an Acidogenesis substrate for volatile fatty acids and simultaneous ethanol production. These products can be used for ester production, which is the new generation biofuel. Therefore, the aims of the present study are (i) to examine any promotional effect of γ-alumina on Acidogenesis of a sucrose-raffinose mixture simulating vinasse, (ii) to study the operational stability of the continuous Acidogenesis of sucrose and raffinose and subsequently of vinasse, and (iii) to determine the volatile fatty acid chemical composition and ethanol formation.
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new generation biofuel continuous Acidogenesis of sucrose raffinose mixture simulating vinasse is promoted by γ alumina pellets
Biotechnology for Biofuels, 2015Co-Authors: Katerina Lappa, Nikolaos Bastas, Stavros Klaoudatos, Argyro Bekatorou, Panagiotis Kandylis, Maria Kanellaki, Nikolaos Athanasopoulos, Athanasios A. KoutinasAbstract:This investigation comprises a contribution on the production of a new generation biofuel using the industrial liquid waste of bioethanol distilleries, known as vinasse. This study focuses on the exploitation of vinasse as an Acidogenesis substrate for volatile fatty acids and simultaneous ethanol production. These products can be used for ester production, which is the new generation biofuel. Therefore, the aims of the present study are (i) to examine any promotional effect of γ-alumina on Acidogenesis of a sucrose-raffinose mixture simulating vinasse, (ii) to study the operational stability of the continuous Acidogenesis of sucrose and raffinose and subsequently of vinasse, and (iii) to determine the volatile fatty acid chemical composition and ethanol formation. Batch Acidogenesis of sucrose and raffinose mixtures showed that γ-alumina promoted fermentation leading to an increase in the volatile fatty acid yield factor from 40% to 95% compared to free cells. The application of the system in continuous mode for more than 3 months showed that the continuous volatile fatty acid productivity obtained was higher than 7 g/L/day. Lactic acid was the predominant acid when sucrose and raffinose were used while butyric acid in the case of vinasse. The highest volatile fatty acid concentration reached was 19 g/L for vinasse. A promoting effect of γ-alumina in acidogenic fermentation of sucrose-raffinose and vinasse is reported. Continuous Acidogenesis of sucrose-raffinose mixtures and vinasse using γ-alumina with immobilized cells showed high operational stability (more than 3 months). These findings result in easy scale up of the process that will produce a high annual added value of $11,000,000 in a daily bioethanol production plant of 50,000 L.
