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Nirmal Singh - One of the best experts on this subject based on the ideXlab platform.
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neuroprotective effect of saponin rich extract of Acorus Calamus l in rat model of chronic constriction injury cci of sciatic nerve induced neuropathic pain
Journal of Ethnopharmacology, 2012Co-Authors: Arunachalam Muthuraman, Nirmal SinghAbstract:Abstract Ethnopharmacological relevance Traditionally, Acorus Calamus has been used for the treatment and management of headache, migraine, body ache and severe inflammatory pain in the Unani, Ayurveda and Indian system of medicine. Aim of the study Present study focuses on the evaluation of saponin rich extract of Acorus Calamus (SRE-AC) in chronic constriction injury (CCI) of sciatic nerve induced neuropathic pain and neuronal functional changes in rats. Materials and methods The pain sensitive tests, i.e., thermal and mechanical hyperalgesia and sciatic functional index test, were performed on the different days, i.e., days 0, 1, 7, 14, and 21. The motor and sensory nerve conduction velocity was also measured on the 21st day. Tissue total protein, superoxide anion generation, total calcium, myeloperoxidase and TNF-α levels were estimated to assess biochemical changes. Histopathological evaluations were also performed. SRE-AC (20 and 40 mg/kg) and pregabalin (10 mg/kg, serving as a positive control) were administered orally for 14 consecutive days from the day of surgery. Results CCI produced significant (P Conclusion SRE-AC has shown ameliorative effect in CCI-induced neuropathic pain which may be attributed to its multiple actions including anti-oxidative, anti-inflammatory and neuroprotective actions.
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Attenuating effect of hydroalcoholic extract of Acorus Calamus in vincristine-induced painful neuropathy in rats
Journal of Natural Medicines, 2011Co-Authors: Arunachalam Muthuraman, Nirmal SinghAbstract:The present study was designed to investigate the attenuating potential of hydroalcoholic extract of Acorus Calamus in vincristine-induced neuropathic pain in rats. Vincristine (50 μg/kg, i.p. for 10 consecutive days) was administered to induce neuropathic pain in rats. Hot plate, plantar, Randall–Selitto and von Frey hair tests were performed to assess the degree of thermal and mechanical hyperalgesia and mechanical allodynia, respectively, at different time intervals, viz., 0, 1, 3, 6, 9, 12, 15, 18 and 21 days. Tissue myeloperoxidase, superoxide anion and total calcium levels were determined after day 21to assess biochemical alterations. Histopathological evaluations were also performed. Hydroalcoholic extract of Acorus Calamus (HAE- AC , 100 and 200 mg/kg, p.o.) and pregabalin (10 mg/kg, p.o.) were administered for 14 consecutive days. Vincristine significantly induced peripheral neuropathic pain, manifested in thermal and mechanical hyperalgesia and mechanical allodynia, along with rises in the levels of superoxide anion, total calcium and myeloperoxidase activity. Moreover, significant histological changes were also observed. HAE- AC attenuated vincristine-induced development of painful behavioural, biochemical and histological changes in a dose-dependent manner comparable to that of pregabalin, serving as positive control. Acorus Calamus prevented vincristine-induced neuropathic pain, which may be attributed to its anti-oxidative, anti-inflammatory, neuroprotective and calcium inhibitory actions, among others.
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Effect of hydroalcoholic extract of Acorus Calamus on tibial and sural nerve transection-induced painful neuropathy in rats
Journal of Natural Medicines, 2011Co-Authors: Arunachalam Muthuraman, Nirmal Singh, Amteshwar S. JaggiAbstract:This study was designed to investigate the therapeutic potential of hydroalcoholic extracts of Acorus Calamus (AC) in tibial and sural nerve transection (TST)-induced neuropathic pain in rats. The hot plate, paw heat allodynia, acetone drop, and pinprick tests were performed to assess the degree of heat hyperalgesia, heat and cold allodynia, and mechanical hyperalgesia, respectively, at different time intervals, i.e., day 0, 1, 3, 6, 9, 12, 15, 18, and 21. The tissue superoxide anion and total calcium were measured as markers of oxidative stress. Tissue myeloperoxidase activity was measured as a specific marker of inflammation. Histopathological evaluation was also performed in the nervous tissue to assess the axonal degeneration. Pregabalin served as positive control in this study. TST in rats significantly induced thermal hyperalgesia and allodynia, mechanical hyperalgesia, and increased the levels of superoxide anion, total calcium, and myeloperoxidase (MPO) activity. Moreover significant histological changes were also observed. Oral administration of AC hydroalcoholic extract (100 and 200 mg/kg for 14 days) attenuated TST-induced behavioral, biochemical, and histological changes. Acorus Calamus has ameliorative potential in TST-induced painful neuropathy, and this effect may be attributed to its multiple actions including anti-inflammatory, antioxidant, and neuroprotective actions.
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attenuating effect of Acorus Calamus extract in chronic constriction injury induced neuropathic pain in rats an evidence of anti oxidative anti inflammatory neuroprotective and calcium inhibitory effects
BMC Complementary and Alternative Medicine, 2011Co-Authors: Arunachalam Muthuraman, Nirmal SinghAbstract:Acorus Calamus (family: Araceae), is an indigenous plant, traditionally it is used as an ingredient of various cocktail preparations and for the management of severe inflammatory disorders in Indian system of medicine. Present study investigated the attenuating role of Acorus Calamus plant extract in chronic constriction injury (CCI) of sciatic nerve induced peripheral neuropathy in rats. Hot plate, plantar, Randall Selitto, Von Frey Hair, pin prick, acetone drop, photoactometer and rota-rod tests were performed to assess degree of thermal, radiant, mechanical, chemical sensation, spontaneous motor activity and motor co-ordination changes respectively, at different time intervals i.e., day 0, 1, 3, 6, 9, 12, 15, 18 and 21. Tissue myeloperoxidase, superoxide anion and total calcium levels were determined after 21st day to assess biochemical alterations. Histopathological evaluations were also performed. Hydroalcoholic extract of Acorus Calamus (HAE-AC, 100 and 200 mg/kg, p.o.) and pregabalin (10 mg/kg, p.o.) were administered from the day of surgery for 14 days. CCI of sciatic nerve significantly induced thermal, radiant, mechanical hyperalgesia and thermal, chemical, tactile allodynia, along with increase in the levels of superoxide anion, total calcium and myeloperoxidase activity. Moreover significant histological changes were also observed. HAE-AC attenuated CCI induced development of painful behavioural, biochemical and histological changes in a dose dependent manner similar to that of pregabalin serving as positive control. Acorus Calamus prevented CCI induced neuropathy which may be attributed to its multiple actions including anti-oxidative, anti-inflammatory, neuroprotective and calcium inhibitory actions.
H S Chandel - One of the best experts on this subject based on the ideXlab platform.
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evaluation of wound healing activity of Acorus Calamus linn
Natural Product Research, 2010Co-Authors: Nilesh Jain, Ruchi Jain, Arti Jain, Deepak Kumar Jain, H S ChandelAbstract:The aim of the present study was to assess the wound-healing activity of ethanolic extracts of Acorus Calamus leaves. A wound was induced by an excision- and incision-based wound model in rats of either sex. The mature green leaves of A. Calamus were collected and authenticated. Extractions of dried leaves were carried out with 80% ethanol in a soxhlet apparatus. For wound-healing activity, the extracts were applied topically once daily in conc. of 40% w/w and 20% w/w in the form of ointment and compared with a standard drug (povidion-iodine). The healing of the wound was assessed by the rate of wound closure, period of epithelialisation, tensile strength and weight of the granulation tissue, hydroxyproline content and histopathology of the granulation tissue. The ethanolic extract of A. Calamus promoted wound-healing activity significantly in both the wound models studied. The histological study of the granulation tissue with 20% A. Calamus extract ointment-treated animals showed a larger number of infla...
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evaluation of wound healing activity of Acorus Calamus linn
Natural Product Research, 2010Co-Authors: Nilesh Jain, Ruchi Jain, Arti Jain, Deepak Kumar Jain, H S ChandelAbstract:The aim of the present study was to assess the wound-healing activity of ethanolic extracts of Acorus Calamus leaves. A wound was induced by an excision- and incision-based wound model in rats of either sex. The mature green leaves of A. Calamus were collected and authenticated. Extractions of dried leaves were carried out with 80% ethanol in a soxhlet apparatus. For wound-healing activity, the extracts were applied topically once daily in conc. of 40% w/w and 20% w/w in the form of ointment and compared with a standard drug (povidion-iodine). The healing of the wound was assessed by the rate of wound closure, period of epithelialisation, tensile strength and weight of the granulation tissue, hydroxyproline content and histopathology of the granulation tissue. The ethanolic extract of A. Calamus promoted wound-healing activity significantly in both the wound models studied. The histological study of the granulation tissue with 20% A. Calamus extract ointment-treated animals showed a larger number of inflammatory cells and lesser collagen when compared with the 40% A. Calamus extract ointment-treated animals. However, this was better than the control group of animals. Enhanced wound contraction, decreased epithelialisation time, increased hydroxyproline content and histological characteristics suggest that A. Calamus extract may have therapeutic benefits in wound healing.
Arunachalam Muthuraman - One of the best experts on this subject based on the ideXlab platform.
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neuroprotective effect of saponin rich extract of Acorus Calamus l in rat model of chronic constriction injury cci of sciatic nerve induced neuropathic pain
Journal of Ethnopharmacology, 2012Co-Authors: Arunachalam Muthuraman, Nirmal SinghAbstract:Abstract Ethnopharmacological relevance Traditionally, Acorus Calamus has been used for the treatment and management of headache, migraine, body ache and severe inflammatory pain in the Unani, Ayurveda and Indian system of medicine. Aim of the study Present study focuses on the evaluation of saponin rich extract of Acorus Calamus (SRE-AC) in chronic constriction injury (CCI) of sciatic nerve induced neuropathic pain and neuronal functional changes in rats. Materials and methods The pain sensitive tests, i.e., thermal and mechanical hyperalgesia and sciatic functional index test, were performed on the different days, i.e., days 0, 1, 7, 14, and 21. The motor and sensory nerve conduction velocity was also measured on the 21st day. Tissue total protein, superoxide anion generation, total calcium, myeloperoxidase and TNF-α levels were estimated to assess biochemical changes. Histopathological evaluations were also performed. SRE-AC (20 and 40 mg/kg) and pregabalin (10 mg/kg, serving as a positive control) were administered orally for 14 consecutive days from the day of surgery. Results CCI produced significant (P Conclusion SRE-AC has shown ameliorative effect in CCI-induced neuropathic pain which may be attributed to its multiple actions including anti-oxidative, anti-inflammatory and neuroprotective actions.
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Attenuating effect of hydroalcoholic extract of Acorus Calamus in vincristine-induced painful neuropathy in rats
Journal of Natural Medicines, 2011Co-Authors: Arunachalam Muthuraman, Nirmal SinghAbstract:The present study was designed to investigate the attenuating potential of hydroalcoholic extract of Acorus Calamus in vincristine-induced neuropathic pain in rats. Vincristine (50 μg/kg, i.p. for 10 consecutive days) was administered to induce neuropathic pain in rats. Hot plate, plantar, Randall–Selitto and von Frey hair tests were performed to assess the degree of thermal and mechanical hyperalgesia and mechanical allodynia, respectively, at different time intervals, viz., 0, 1, 3, 6, 9, 12, 15, 18 and 21 days. Tissue myeloperoxidase, superoxide anion and total calcium levels were determined after day 21to assess biochemical alterations. Histopathological evaluations were also performed. Hydroalcoholic extract of Acorus Calamus (HAE- AC , 100 and 200 mg/kg, p.o.) and pregabalin (10 mg/kg, p.o.) were administered for 14 consecutive days. Vincristine significantly induced peripheral neuropathic pain, manifested in thermal and mechanical hyperalgesia and mechanical allodynia, along with rises in the levels of superoxide anion, total calcium and myeloperoxidase activity. Moreover, significant histological changes were also observed. HAE- AC attenuated vincristine-induced development of painful behavioural, biochemical and histological changes in a dose-dependent manner comparable to that of pregabalin, serving as positive control. Acorus Calamus prevented vincristine-induced neuropathic pain, which may be attributed to its anti-oxidative, anti-inflammatory, neuroprotective and calcium inhibitory actions, among others.
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Effect of hydroalcoholic extract of Acorus Calamus on tibial and sural nerve transection-induced painful neuropathy in rats
Journal of Natural Medicines, 2011Co-Authors: Arunachalam Muthuraman, Nirmal Singh, Amteshwar S. JaggiAbstract:This study was designed to investigate the therapeutic potential of hydroalcoholic extracts of Acorus Calamus (AC) in tibial and sural nerve transection (TST)-induced neuropathic pain in rats. The hot plate, paw heat allodynia, acetone drop, and pinprick tests were performed to assess the degree of heat hyperalgesia, heat and cold allodynia, and mechanical hyperalgesia, respectively, at different time intervals, i.e., day 0, 1, 3, 6, 9, 12, 15, 18, and 21. The tissue superoxide anion and total calcium were measured as markers of oxidative stress. Tissue myeloperoxidase activity was measured as a specific marker of inflammation. Histopathological evaluation was also performed in the nervous tissue to assess the axonal degeneration. Pregabalin served as positive control in this study. TST in rats significantly induced thermal hyperalgesia and allodynia, mechanical hyperalgesia, and increased the levels of superoxide anion, total calcium, and myeloperoxidase (MPO) activity. Moreover significant histological changes were also observed. Oral administration of AC hydroalcoholic extract (100 and 200 mg/kg for 14 days) attenuated TST-induced behavioral, biochemical, and histological changes. Acorus Calamus has ameliorative potential in TST-induced painful neuropathy, and this effect may be attributed to its multiple actions including anti-inflammatory, antioxidant, and neuroprotective actions.
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attenuating effect of Acorus Calamus extract in chronic constriction injury induced neuropathic pain in rats an evidence of anti oxidative anti inflammatory neuroprotective and calcium inhibitory effects
BMC Complementary and Alternative Medicine, 2011Co-Authors: Arunachalam Muthuraman, Nirmal SinghAbstract:Acorus Calamus (family: Araceae), is an indigenous plant, traditionally it is used as an ingredient of various cocktail preparations and for the management of severe inflammatory disorders in Indian system of medicine. Present study investigated the attenuating role of Acorus Calamus plant extract in chronic constriction injury (CCI) of sciatic nerve induced peripheral neuropathy in rats. Hot plate, plantar, Randall Selitto, Von Frey Hair, pin prick, acetone drop, photoactometer and rota-rod tests were performed to assess degree of thermal, radiant, mechanical, chemical sensation, spontaneous motor activity and motor co-ordination changes respectively, at different time intervals i.e., day 0, 1, 3, 6, 9, 12, 15, 18 and 21. Tissue myeloperoxidase, superoxide anion and total calcium levels were determined after 21st day to assess biochemical alterations. Histopathological evaluations were also performed. Hydroalcoholic extract of Acorus Calamus (HAE-AC, 100 and 200 mg/kg, p.o.) and pregabalin (10 mg/kg, p.o.) were administered from the day of surgery for 14 days. CCI of sciatic nerve significantly induced thermal, radiant, mechanical hyperalgesia and thermal, chemical, tactile allodynia, along with increase in the levels of superoxide anion, total calcium and myeloperoxidase activity. Moreover significant histological changes were also observed. HAE-AC attenuated CCI induced development of painful behavioural, biochemical and histological changes in a dose dependent manner similar to that of pregabalin serving as positive control. Acorus Calamus prevented CCI induced neuropathy which may be attributed to its multiple actions including anti-oxidative, anti-inflammatory, neuroprotective and calcium inhibitory actions.
Nilesh Jain - One of the best experts on this subject based on the ideXlab platform.
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evaluation of wound healing activity of Acorus Calamus linn
Natural Product Research, 2010Co-Authors: Nilesh Jain, Ruchi Jain, Arti Jain, Deepak Kumar Jain, H S ChandelAbstract:The aim of the present study was to assess the wound-healing activity of ethanolic extracts of Acorus Calamus leaves. A wound was induced by an excision- and incision-based wound model in rats of either sex. The mature green leaves of A. Calamus were collected and authenticated. Extractions of dried leaves were carried out with 80% ethanol in a soxhlet apparatus. For wound-healing activity, the extracts were applied topically once daily in conc. of 40% w/w and 20% w/w in the form of ointment and compared with a standard drug (povidion-iodine). The healing of the wound was assessed by the rate of wound closure, period of epithelialisation, tensile strength and weight of the granulation tissue, hydroxyproline content and histopathology of the granulation tissue. The ethanolic extract of A. Calamus promoted wound-healing activity significantly in both the wound models studied. The histological study of the granulation tissue with 20% A. Calamus extract ointment-treated animals showed a larger number of infla...
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evaluation of wound healing activity of Acorus Calamus linn
Natural Product Research, 2010Co-Authors: Nilesh Jain, Ruchi Jain, Arti Jain, Deepak Kumar Jain, H S ChandelAbstract:The aim of the present study was to assess the wound-healing activity of ethanolic extracts of Acorus Calamus leaves. A wound was induced by an excision- and incision-based wound model in rats of either sex. The mature green leaves of A. Calamus were collected and authenticated. Extractions of dried leaves were carried out with 80% ethanol in a soxhlet apparatus. For wound-healing activity, the extracts were applied topically once daily in conc. of 40% w/w and 20% w/w in the form of ointment and compared with a standard drug (povidion-iodine). The healing of the wound was assessed by the rate of wound closure, period of epithelialisation, tensile strength and weight of the granulation tissue, hydroxyproline content and histopathology of the granulation tissue. The ethanolic extract of A. Calamus promoted wound-healing activity significantly in both the wound models studied. The histological study of the granulation tissue with 20% A. Calamus extract ointment-treated animals showed a larger number of inflammatory cells and lesser collagen when compared with the 40% A. Calamus extract ointment-treated animals. However, this was better than the control group of animals. Enhanced wound contraction, decreased epithelialisation time, increased hydroxyproline content and histological characteristics suggest that A. Calamus extract may have therapeutic benefits in wound healing.
Andrea Scorciapino - One of the best experts on this subject based on the ideXlab platform.
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chemical composition of the essential oil and supercritical co2 extract of commiphora myrrha nees engl and of Acorus Calamus l
Journal of Agricultural and Food Chemistry, 2005Co-Authors: Bruno Marongiu, Alessandra Piras, Silvia Porcedda, Andrea ScorciapinoAbstract:Volatile concentrates from the oleo-gum resin of Commiphora myrrha (Nees) Engl. and from the rhizomes of Acorus Calamus were isolated by supercritical extraction with carbon dioxide. The volatile oil of myrrh was obtained at 9.0 MPa and 50 °C and at a CO2 flow of 1.5 kg/h. Acorus Calamus was extracted at 9.0 MPa and 45 °C and at a CO2 flow of 1.6 kg/h. In both cases, an oil devoid of cuticular waxes was obtained with a single depressurization stage. The SFE myrrh oil had a yield, Y, of 3.2%. Its main components, identified and quantified by GC/MS, were furanoeudesma-1,3-diene, 34.9%; lindestrene, 12.9%; curzerene, 8.5%; and germacrone, 5.8%. The essential oils from the same starting material by hydrodistillation, HD, (Y = 2.8%) and by steam distillation, SD, (Y = 0.4%) were quite similar to the SFE extract. The main components of the SFE oil of A. Calamus (Y = 3.5%) were acorenone, 13.4%; iso-acorone, 11.6%; (Z)-sesquilavandulol, 11.0%; dehydroxy isocalamendiol, 7.7%; and β-asarone, 5.5%. The comparison w...
