The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
Scott R Manalis - One of the best experts on this subject based on the ideXlab platform.
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noninvasive monitoring of single cell mechanics by Acoustic Scattering
Nature Methods, 2019Co-Authors: Joon Ho Kang, Teemu P Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S Doyle, Scott R ManalisAbstract:The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via Acoustic Scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized Acoustic Scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that Acoustic Scattering can be used to noninvasively probe subtle and transient dynamics. Acoustic Scattering in a suspended microchannel resonator can be used to measure mechanical properties of single cells in a noninvasive manner. The approach is applied to follow stiffness changes of individual cells throughout the cell cycle.
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Noninvasive monitoring of single-cell mechanics by Acoustic Scattering.
Nature Methods, 2019Co-Authors: Joon Ho Kang, Teemu P Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S Doyle, Scott R ManalisAbstract:The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via Acoustic Scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of
Teemu P Miettinen - One of the best experts on this subject based on the ideXlab platform.
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noninvasive monitoring of single cell mechanics by Acoustic Scattering
Nature Methods, 2019Co-Authors: Joon Ho Kang, Teemu P Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S Doyle, Scott R ManalisAbstract:The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via Acoustic Scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized Acoustic Scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that Acoustic Scattering can be used to noninvasively probe subtle and transient dynamics. Acoustic Scattering in a suspended microchannel resonator can be used to measure mechanical properties of single cells in a noninvasive manner. The approach is applied to follow stiffness changes of individual cells throughout the cell cycle.
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Noninvasive monitoring of single-cell mechanics by Acoustic Scattering.
Nature Methods, 2019Co-Authors: Joon Ho Kang, Teemu P Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S Doyle, Scott R ManalisAbstract:The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via Acoustic Scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of
Joon Ho Kang - One of the best experts on this subject based on the ideXlab platform.
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noninvasive monitoring of single cell mechanics by Acoustic Scattering
Nature Methods, 2019Co-Authors: Joon Ho Kang, Teemu P Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S Doyle, Scott R ManalisAbstract:The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via Acoustic Scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized Acoustic Scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that Acoustic Scattering can be used to noninvasively probe subtle and transient dynamics. Acoustic Scattering in a suspended microchannel resonator can be used to measure mechanical properties of single cells in a noninvasive manner. The approach is applied to follow stiffness changes of individual cells throughout the cell cycle.
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Noninvasive monitoring of single-cell mechanics by Acoustic Scattering.
Nature Methods, 2019Co-Authors: Joon Ho Kang, Teemu P Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S Doyle, Scott R ManalisAbstract:The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via Acoustic Scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of
Georgios Katsikis - One of the best experts on this subject based on the ideXlab platform.
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noninvasive monitoring of single cell mechanics by Acoustic Scattering
Nature Methods, 2019Co-Authors: Joon Ho Kang, Teemu P Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S Doyle, Scott R ManalisAbstract:The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via Acoustic Scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized Acoustic Scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that Acoustic Scattering can be used to noninvasively probe subtle and transient dynamics. Acoustic Scattering in a suspended microchannel resonator can be used to measure mechanical properties of single cells in a noninvasive manner. The approach is applied to follow stiffness changes of individual cells throughout the cell cycle.
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Noninvasive monitoring of single-cell mechanics by Acoustic Scattering.
Nature Methods, 2019Co-Authors: Joon Ho Kang, Teemu P Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S Doyle, Scott R ManalisAbstract:The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via Acoustic Scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of
Patrick S Doyle - One of the best experts on this subject based on the ideXlab platform.
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noninvasive monitoring of single cell mechanics by Acoustic Scattering
Nature Methods, 2019Co-Authors: Joon Ho Kang, Teemu P Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S Doyle, Scott R ManalisAbstract:The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via Acoustic Scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized Acoustic Scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that Acoustic Scattering can be used to noninvasively probe subtle and transient dynamics. Acoustic Scattering in a suspended microchannel resonator can be used to measure mechanical properties of single cells in a noninvasive manner. The approach is applied to follow stiffness changes of individual cells throughout the cell cycle.
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Noninvasive monitoring of single-cell mechanics by Acoustic Scattering.
Nature Methods, 2019Co-Authors: Joon Ho Kang, Teemu P Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S Doyle, Scott R ManalisAbstract:The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via Acoustic Scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of
