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Activity Thermodynamic

The Experts below are selected from a list of 123 Experts worldwide ranked by ideXlab platform

Andras Foldesi – 1st expert on this subject based on the ideXlab platform

  • carba lna 5mec a g t modified oligos show nucleobase specific modulation of 3 exonuclease Activity Thermodynamic stability rna selectivity and rnase h elicitation synthesis and biochemistry
    Journal of Organic Chemistry, 2011
    Co-Authors: Ramshankar Upadhayaya, Sachin Gangadhar Deshpande, Qing Li, Ramakant A Kardile, Aftab Y Sayyed, Eknath Kamalakar Kshirsagar, Rahul Vilas Salunke, Shailesh S Dixit, Chuanzheng Zhou, Andras Foldesi

    Abstract:

    Using the intramolecular 5-exo-5-hexenyl radical as a key cyclization step, we previously reported an unambiguous synthesis of carba-LNA thymine (cLNA-T), which we subsequently incorporated in antisense oligonucleotides (AON) and investigated their biochemical properties [J. Am. Chem. Soc.2007, 129 (26), 8362–8379]. These cLNA-T incorporated oligos showed specific RNA affinity of +3.5–5 °C/modification for AON:RNA heteroduplexes, which is comparable to what is found for those of LNAs (Locked Nucleic Acids). These modified oligos however showed significantly enhanced nuclease stability (ca. 100 times more) in the blood serum compared to those of the LNA modified counterparts without compromising any RNase H recruitment capability. We herein report the synthesis of 5-methylcytosine-1-yl (MeC), 9-adeninyl (A), and 9-guaninyl (G) derivatives of cLNA and their oligonucleotides and report their biochemical properties as potential RNA-directed inhibitors. In a series of isosequential carba-LNA modified AONs, we …

  • Carba-LNA-5MeC/A/G/T modified oligos show nucleobase-specific modulation of 3′-exonuclease Activity, Thermodynamic stability, RNA selectivity, and RNase H elicitation: synthesis and biochemistry.
    Journal of Organic Chemistry, 2011
    Co-Authors: Ramshankar Upadhayaya, Sachin Gangadhar Deshpande, Qing Li, Ramakant A Kardile, Aftab Y Sayyed, Eknath Kamalakar Kshirsagar, Rahul Vilas Salunke, Shailesh S Dixit, Chuanzheng Zhou, Andras Foldesi

    Abstract:

    Using the intramolecular 5-exo-5-hexenyl radical as a key cyclization step, we previously reported an unambiguous synthesis of carba-LNA thymine (cLNA-T), which we subsequently incorporated in antisense oligonucleotides (AON) and investigated their biochemical properties [J. Am. Chem. Soc.2007, 129 (26), 8362–8379]. These cLNA-T incorporated oligos showed specific RNA affinity of +3.5–5 °C/modification for AON:RNA heteroduplexes, which is comparable to what is found for those of LNAs (Locked Nucleic Acids). These modified oligos however showed significantly enhanced nuclease stability (ca. 100 times more) in the blood serum compared to those of the LNA modified counterparts without compromising any RNase H recruitment capability. We herein report the synthesis of 5-methylcytosine-1-yl (MeC), 9-adeninyl (A), and 9-guaninyl (G) derivatives of cLNA and their oligonucleotides and report their biochemical properties as potential RNA-directed inhibitors. In a series of isosequential carba-LNA modified AONs, we …

Ramshankar Upadhayaya – 2nd expert on this subject based on the ideXlab platform

  • carba lna 5mec a g t modified oligos show nucleobase specific modulation of 3 exonuclease Activity Thermodynamic stability rna selectivity and rnase h elicitation synthesis and biochemistry
    Journal of Organic Chemistry, 2011
    Co-Authors: Ramshankar Upadhayaya, Sachin Gangadhar Deshpande, Qing Li, Ramakant A Kardile, Aftab Y Sayyed, Eknath Kamalakar Kshirsagar, Rahul Vilas Salunke, Shailesh S Dixit, Chuanzheng Zhou, Andras Foldesi

    Abstract:

    Using the intramolecular 5-exo-5-hexenyl radical as a key cyclization step, we previously reported an unambiguous synthesis of carba-LNA thymine (cLNA-T), which we subsequently incorporated in antisense oligonucleotides (AON) and investigated their biochemical properties [J. Am. Chem. Soc.2007, 129 (26), 8362–8379]. These cLNA-T incorporated oligos showed specific RNA affinity of +3.5–5 °C/modification for AON:RNA heteroduplexes, which is comparable to what is found for those of LNAs (Locked Nucleic Acids). These modified oligos however showed significantly enhanced nuclease stability (ca. 100 times more) in the blood serum compared to those of the LNA modified counterparts without compromising any RNase H recruitment capability. We herein report the synthesis of 5-methylcytosine-1-yl (MeC), 9-adeninyl (A), and 9-guaninyl (G) derivatives of cLNA and their oligonucleotides and report their biochemical properties as potential RNA-directed inhibitors. In a series of isosequential carba-LNA modified AONs, we …

  • Carba-LNA-5MeC/A/G/T modified oligos show nucleobase-specific modulation of 3′-exonuclease Activity, Thermodynamic stability, RNA selectivity, and RNase H elicitation: synthesis and biochemistry.
    Journal of Organic Chemistry, 2011
    Co-Authors: Ramshankar Upadhayaya, Sachin Gangadhar Deshpande, Qing Li, Ramakant A Kardile, Aftab Y Sayyed, Eknath Kamalakar Kshirsagar, Rahul Vilas Salunke, Shailesh S Dixit, Chuanzheng Zhou, Andras Foldesi

    Abstract:

    Using the intramolecular 5-exo-5-hexenyl radical as a key cyclization step, we previously reported an unambiguous synthesis of carba-LNA thymine (cLNA-T), which we subsequently incorporated in antisense oligonucleotides (AON) and investigated their biochemical properties [J. Am. Chem. Soc.2007, 129 (26), 8362–8379]. These cLNA-T incorporated oligos showed specific RNA affinity of +3.5–5 °C/modification for AON:RNA heteroduplexes, which is comparable to what is found for those of LNAs (Locked Nucleic Acids). These modified oligos however showed significantly enhanced nuclease stability (ca. 100 times more) in the blood serum compared to those of the LNA modified counterparts without compromising any RNase H recruitment capability. We herein report the synthesis of 5-methylcytosine-1-yl (MeC), 9-adeninyl (A), and 9-guaninyl (G) derivatives of cLNA and their oligonucleotides and report their biochemical properties as potential RNA-directed inhibitors. In a series of isosequential carba-LNA modified AONs, we …

Maria Grzeszczuk – 3rd expert on this subject based on the ideXlab platform

  • Electrocrystallization of silver nanoparticles from silver halides in polypyrrole evidenced by their SERS ActivityThermodynamic and kinetic conditions
    Journal of Solid State Electrochemistry, 2018
    Co-Authors: Kamil Wójcik, Tomasz Czaja, Roman Szostak, Maria Grzeszczuk

    Abstract:

    Silver(I) halide particles embodied in polypyrrole matrices are synthesized and further processed electrochemically to get nanoparticles of silver with significant Activity in the surface-enhanced Raman spectroscopy (SERS) of the rhodamine 6G test solute, which is considered as a clear evidence of Ag nanocrystallization. The electrocrystallization of Ag nanoparticles is analyzed using redox charges of composites determined from cyclic voltammograms recorded at various time scales and/or cycle numbers. Pristine PPy/AgX and modified PPy/AgX/AgNP composites are characterized using electrochemical impedance spectroscopy, scanning electron microscopy, and X-ray electron dispersive spectroscopy. The Thermodynamic and kinetic conditions of the electrochemical modification of pristine polypyrrole–AgX composites leading to the electrocrystallization of Ag nanoparticles are discussed.