The Experts below are selected from a list of 116199 Experts worldwide ranked by ideXlab platform
J V Garcia - One of the best experts on this subject based on the ideXlab platform.
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engraftment of nod scid mice with human cd34 cells transduced by concentrated oncoretroviral Vector particles pseudotyped with the feline endogenous retrovirus rd114 envelope protein
Journal of Virology, 2001Co-Authors: Joel Gatlin, Michael W Melkus, Angela Padgett, P F Kelly, J V GarciaAbstract:Oncoretrovirus Vectors pseudotyped with the feline endogenous retrovirus (RD114) envelope protein produced by the FLYRD18 packaging cell line have previously been shown to transduce human hematopoietic progenitor cells with a greater efficiency than similar amphotropic envelope-pseudotyped Vectors. In this report, we describe the production and efficient concentration of RD114-pseudotyped murine leukemia virus (MLV)-based Vectors. Following a single round of centrifugation, Vector supernatants were concentrated approximately 200-fold with a 50 to 70% yield. Concentrated Vector stocks transduced prestimulated human CD34+ (hCD34+) cells with approximately 69% efficiency (n = 7, standard deviation = 4.4%) using a single Addition of Vector at a low multiplicity of infection (MOI = 5). Introduction of transduced hCD34+ cells into irradiated NOD/SCID recipients resulted in multilineage engraftment with long-term transgene expression. These data demonstrate that RD114-pseudotyped MLV-based Vectors can be efficiently concentrated to high titers and that hCD34+ cells transduced with concentrated Vector stocks retain in vivo repopulating potential. These results highlight the potential of RD114-pseudotyped oncoretrovirus Vectors for future clinical implementation in hematopoietic stem cell gene transfer.
Sabyasachi Ghosh - One of the best experts on this subject based on the ideXlab platform.
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impact of different extended components of mean field models on transport coefficients of quark matter
arXiv: Nuclear Theory, 2019Co-Authors: Chowdhury Aminul Islam, Jayanta Dey, Sabyasachi GhoshAbstract:The transport coefficients like shear viscosity, electrical and thermal conductivities are critically analyzed in the framework of Nambu\textendash\,Jona-Lasinio (NJL) and its different extensions like Addition of Vector interaction, Polyakov loop extended version (PNJL) and the entangled PNJL (EPNJL) models. We have considered the standard expressions of transport coefficients, obtained in relaxation time approximation of kinetic theory. Using a constant relaxation time, we first observe the changes of thermodynamical phase space factor for different versions of models and then their corresponding temperature dependent relaxation times are estimated. Impact of different components of model on fluidity is critically investigated.
Joel Gatlin - One of the best experts on this subject based on the ideXlab platform.
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engraftment of nod scid mice with human cd34 cells transduced by concentrated oncoretroviral Vector particles pseudotyped with the feline endogenous retrovirus rd114 envelope protein
Journal of Virology, 2001Co-Authors: Joel Gatlin, Michael W Melkus, Angela Padgett, P F Kelly, J V GarciaAbstract:Oncoretrovirus Vectors pseudotyped with the feline endogenous retrovirus (RD114) envelope protein produced by the FLYRD18 packaging cell line have previously been shown to transduce human hematopoietic progenitor cells with a greater efficiency than similar amphotropic envelope-pseudotyped Vectors. In this report, we describe the production and efficient concentration of RD114-pseudotyped murine leukemia virus (MLV)-based Vectors. Following a single round of centrifugation, Vector supernatants were concentrated approximately 200-fold with a 50 to 70% yield. Concentrated Vector stocks transduced prestimulated human CD34+ (hCD34+) cells with approximately 69% efficiency (n = 7, standard deviation = 4.4%) using a single Addition of Vector at a low multiplicity of infection (MOI = 5). Introduction of transduced hCD34+ cells into irradiated NOD/SCID recipients resulted in multilineage engraftment with long-term transgene expression. These data demonstrate that RD114-pseudotyped MLV-based Vectors can be efficiently concentrated to high titers and that hCD34+ cells transduced with concentrated Vector stocks retain in vivo repopulating potential. These results highlight the potential of RD114-pseudotyped oncoretrovirus Vectors for future clinical implementation in hematopoietic stem cell gene transfer.
Chowdhury Aminul Islam - One of the best experts on this subject based on the ideXlab platform.
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impact of different extended components of mean field models on transport coefficients of quark matter
arXiv: Nuclear Theory, 2019Co-Authors: Chowdhury Aminul Islam, Jayanta Dey, Sabyasachi GhoshAbstract:The transport coefficients like shear viscosity, electrical and thermal conductivities are critically analyzed in the framework of Nambu\textendash\,Jona-Lasinio (NJL) and its different extensions like Addition of Vector interaction, Polyakov loop extended version (PNJL) and the entangled PNJL (EPNJL) models. We have considered the standard expressions of transport coefficients, obtained in relaxation time approximation of kinetic theory. Using a constant relaxation time, we first observe the changes of thermodynamical phase space factor for different versions of models and then their corresponding temperature dependent relaxation times are estimated. Impact of different components of model on fluidity is critically investigated.
Michael W Melkus - One of the best experts on this subject based on the ideXlab platform.
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engraftment of nod scid mice with human cd34 cells transduced by concentrated oncoretroviral Vector particles pseudotyped with the feline endogenous retrovirus rd114 envelope protein
Journal of Virology, 2001Co-Authors: Joel Gatlin, Michael W Melkus, Angela Padgett, P F Kelly, J V GarciaAbstract:Oncoretrovirus Vectors pseudotyped with the feline endogenous retrovirus (RD114) envelope protein produced by the FLYRD18 packaging cell line have previously been shown to transduce human hematopoietic progenitor cells with a greater efficiency than similar amphotropic envelope-pseudotyped Vectors. In this report, we describe the production and efficient concentration of RD114-pseudotyped murine leukemia virus (MLV)-based Vectors. Following a single round of centrifugation, Vector supernatants were concentrated approximately 200-fold with a 50 to 70% yield. Concentrated Vector stocks transduced prestimulated human CD34+ (hCD34+) cells with approximately 69% efficiency (n = 7, standard deviation = 4.4%) using a single Addition of Vector at a low multiplicity of infection (MOI = 5). Introduction of transduced hCD34+ cells into irradiated NOD/SCID recipients resulted in multilineage engraftment with long-term transgene expression. These data demonstrate that RD114-pseudotyped MLV-based Vectors can be efficiently concentrated to high titers and that hCD34+ cells transduced with concentrated Vector stocks retain in vivo repopulating potential. These results highlight the potential of RD114-pseudotyped oncoretrovirus Vectors for future clinical implementation in hematopoietic stem cell gene transfer.