The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Kenichiro Matsuzaki - One of the best experts on this subject based on the ideXlab platform.
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osteoclast differentiation factor odf induces osteoclast like Cell formation in human peripheral blood mononuclear Cell cultures
Biochemical and Biophysical Research Communications, 1998Co-Authors: Tomonori Morinaga, Nobuyuki Udagawa, Hisataka Yasuda, Kyoji Yamaguchi, Nobuyuki Shima, Kenichiro Matsuzaki, Naoyuki Takahashi, Yoshiaki Toyama, Yutaka YabeAbstract:Abstract We have reported that osteoclast differentiation factor (ODF) expressed on the plasma membrane of osteoblasts/stromal Cells is a ligand for osteoclastogenesis inhibitory factor (OCIF). A genetically engineered soluble form of ODF (sODF) induced osteoclast-like multinucleated Cells (OCLs) in the presence of M-CSF in mouse spleen Cell cultures. Osteoblasts/stromal Cells were not required in this process. To elucidate the mechanism of human osteoclastogenesis, human peripheral blood mononuclear Cells (PBMCs) were cultured for 7 days with sODF and human M-CSF in the presence or absence of dexamethasone. Treatment of human PBMCs with sODF together with M-CSF induced OCLs, which expressed tartrate-resistant acid phosphatase and vitronectin receptors, produced cAMP in response to calcitonin, and formed resorption pits on dentine slices. OCLs were also formed from the Adherent Cell population of human PBMCs. Dexamethasone was required for human OCL formation in culture of whole PBMCs but not in culture of the Adherent Cell population. OCL formation was strongly inhibited by OCIF simultaneously added. These results clearly indicate that like in mouse osteoclastogenesis, ODF is a critical factor for human osteoclastogenesis. The present study also indicates that OCIF acts as a naturally occurring decoy receptor for ODF in inhibiting signal transduction in human osteoclast formation.
Yutaka Yabe - One of the best experts on this subject based on the ideXlab platform.
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osteoclast differentiation factor odf induces osteoclast like Cell formation in human peripheral blood mononuclear Cell cultures
Biochemical and Biophysical Research Communications, 1998Co-Authors: Tomonori Morinaga, Nobuyuki Udagawa, Hisataka Yasuda, Kyoji Yamaguchi, Nobuyuki Shima, Kenichiro Matsuzaki, Naoyuki Takahashi, Yoshiaki Toyama, Yutaka YabeAbstract:Abstract We have reported that osteoclast differentiation factor (ODF) expressed on the plasma membrane of osteoblasts/stromal Cells is a ligand for osteoclastogenesis inhibitory factor (OCIF). A genetically engineered soluble form of ODF (sODF) induced osteoclast-like multinucleated Cells (OCLs) in the presence of M-CSF in mouse spleen Cell cultures. Osteoblasts/stromal Cells were not required in this process. To elucidate the mechanism of human osteoclastogenesis, human peripheral blood mononuclear Cells (PBMCs) were cultured for 7 days with sODF and human M-CSF in the presence or absence of dexamethasone. Treatment of human PBMCs with sODF together with M-CSF induced OCLs, which expressed tartrate-resistant acid phosphatase and vitronectin receptors, produced cAMP in response to calcitonin, and formed resorption pits on dentine slices. OCLs were also formed from the Adherent Cell population of human PBMCs. Dexamethasone was required for human OCL formation in culture of whole PBMCs but not in culture of the Adherent Cell population. OCL formation was strongly inhibited by OCIF simultaneously added. These results clearly indicate that like in mouse osteoclastogenesis, ODF is a critical factor for human osteoclastogenesis. The present study also indicates that OCIF acts as a naturally occurring decoy receptor for ODF in inhibiting signal transduction in human osteoclast formation.
Corinne A Hoesli - One of the best experts on this subject based on the ideXlab platform.
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expansion and characterization of mesenchymal stem Cells and other anchorage dependent Cell types in fluoropolymer bags treated for Adherent Cell culture
Cytotherapy, 2020Co-Authors: N Zeidan, O Bowden, Natalie Fekete, R Pytel, Pierreluc Girardlauriault, Corinne A HoesliAbstract:Background & Aim Modern Cell based therapies continue to gain momentum as demonstrated by the growing number of clinical trials and Cell therapy commercialization centers. A growing shift towards closed manufacturing systems to replace conventional functionally open systems such as tissue culture polystyrene (TCPS) flasks has prompted the development of various oxygen-permeable Cell culture bags made from materials such as fluorinated ethylene propylene (FEP). However, hydrophobic materials such as untreated TCPS or FEP do not support adequately the growth of anchorage-dependent Cells. For this reason, most TCPS vessels sold for Adherent culture undergo plasma or liquid based surface treatments which increase surface wettability and Cell adhesion. Unlike TCPS, the effect of FEP vessels treated for Adherent culture on Cell adhesion is poorly documented. This is a major obstacle for the production of anchorage-dependent Cell based therapies in a closed setting. The objective of this study was to investigate the impact of the treated form of FEP on Adherent Cell cultures. Methods, Results & Conclusion Different types of anchorage-dependent Cells, including mesenchymal stem Cells (MSCs), mouse insulinoma 6 (MIN6) Cells and human embryonic kidney (HEK) Cells, were cultured in treated-FEP (VueLife® AC Series) bags and their biological properties were analyzed in comparison to Cells seeded on untreated-FEP (VueLife® C Series) and/or treated-TCPS (Nunclon™ Delta-treated T-flasks). Light microscopy revealed that surface treatment enhanced adhesion on FEP, but final Adherent Cell yields after expansion remained lower than on treated-TCPS. Additionally, we observed that surface treatment of FEP did not affect the differentiation status of expanded MSCs or MIN6 populations. As expected, the removal of proteins from the medium or integrin inhibition reduced Cell adhesion. Overall, our results demonstrate that a wide range of anchorage-dependent Cells, including primary Cells (MSCs) and immortalized Cell lines (anchorage-dependent HEK Cells, MIN6) can be successfully expanded in VueLife® AC bags. As an increasing number of adhesion Cell based therapies are ready to enter the clinic, there is a pressing need to find the appropriate Cell culture vessels and materials for better clinical outcomes.
Ningsun Yang - One of the best experts on this subject based on the ideXlab platform.
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establishment of an Adherent Cell feeder layer from human umbilical cord blood for support of long term hematopoietic progenitor Cell growth
Proceedings of the National Academy of Sciences of the United States of America, 1994Co-Authors: Zhenqing Ye, J K Burkholder, J C Schultz, N T Shahidi, Ningsun YangAbstract:Abstract Previous attempts to establish a stromal Cell feeder layer from human umbilical cord blood (HUCB) have met with very limited success. It has been suggested that there is an insufficient number of stromal precursor Cells in HUCB to form a hematopoietic-supporting feeder layer in primary cultures. The present study shows that HUCB does contain a significant accessory Cell population that routinely develops into a confluent, Adherent Cell layer under defined primary culture conditions. HUCB-derived Adherent layers were shown to support long-term hematopoietic activity for an average of 4 months. This was achieved by using a customized coverslip with a modified surface structure as the Cell attachment substratum and using a specialized culture feeding regime. We have characterized the various Cell types (including fibroblasts, macrophages, and endothelial Cells) and extraCellular matrix proteins (including fibronectin, collagen III, and laminin) that were present in abundance in the HUCB-derived Adherent Cell layer. In contrast, oil red O-staining fat Cells were rarely detected. ELISA and bioassays showed that stem Cell factor and interleukin 6 were produced by the HUCB stromal Cell cultures, but interleukin 3 or granulocyte/macrophage colony-stimulating factor was not detected. Application of this hematopoietic culture system to transgenic and gene therapy studies of stem Cells is discussed.
Jun Qiu - One of the best experts on this subject based on the ideXlab platform.
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a noninvasive approach to determine viscoelastic properties of an individual Adherent Cell under fluid flow
Journal of Biomechanics, 2014Co-Authors: Jun Qiu, Andrew D Baik, Elizabeth M C Hillman, Zhuo Zhuang, Cheng Dong, Edward X GuoAbstract:Abstract Mechanical properties of Cells play an important role in their interaction with the extraCellular matrix as well as the mechanotransduction process. Several in vitro techniques have been developed to determine the mechanical properties of Cells, but none of them can measure the viscoelastic properties of an individual Adherent Cell in fluid flow non-invasively. In this study, techniques of fluid–structure interaction (FSI) finite element method and quasi-3-dimensional (quasi-3D) Cell microscopy were innovatively applied to the frequently used flow chamber experiment, where an Adherent Cell was subjected to fluid flow. A new non-invasive approach, with Cells at close to physiological conditions, was established to determine the viscoelastic properties of individual Cells. The results showed an instantaneous modulus of osteocytes of 0.49±0.11 kPa, an equilibrium modulus of 0.31±0.044 kPa, and an apparent viscosity coefficient of 4.07±1.23 kPa s. This new quantitative approach not only provides an exCellent means to measure Cell mechanical properties, but also may help to elucidate the mechanotransduction mechanisms for a variety of Cells under fluid flow stimulation.