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Adrenal Cortex
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Antoine Martinez – One of the best experts on this subject based on the ideXlab platform.
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a transgenic mouse line with specific cre recombinase expression in the Adrenal Cortex
Molecular and Cellular Endocrinology, 2009Co-Authors: Sarah Lambertlanglais, Pierre Val, Samuel Guyot, Bruno Ragazzon, Isabelle Sahutbarnola, Angelique De Haze, Annemarie Lefrancoismartinez, Antoine MartinezAbstract:Abstract The Cre-loxP system combined with gene targeting strategies has proven to be very useful for gene inactivation in specific tissues and/or cell types. To achieve Adrenal Cortex specific recombination in vivo, we used a 0.5-kb fragment of the 5′-flanking region of the akr1b7 gene to drive Cre expression in adrenocortical cells. The resulting 0.5 akr1b7-Cre mice express Cre in all steroidogenic zones of the Adrenal Cortex but not in the gonads. Although recombination of the ROSA26R reporter locus was not observed in all cortical cells, we provide evidence that Cre is expressed in all the cells of the Cortex in adult mice. In addition, Cre activity was found in collecting ducts and maturing glomeruli of the kidney. This line is the first to show specific Cre expression in the Adrenal Cortex in the absence of Cre expression in the gonads. This transgene thus provides a valuable tool for specific gene recombination in the Adrenal Cortex and kidney.
Gary D. Hammer – One of the best experts on this subject based on the ideXlab platform.
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Development of Adrenal Cortex Zonation
Endocrinology and metabolism clinics of North America, 2015Co-Authors: Yewei Xing, Antonio M. Lerario, William E. Rainey, Gary D. HammerAbstract:The human adult Adrenal Cortex is composed of the zona glomerulosa (zG), zona fasciculata (zF), and zona reticularis (zR), which are responsible for production of mineralocorticoids, glucocorticoids, and Adrenal androgens, respectively. The final completion of cortical zonation in humans does not occur until puberty with the establishment of the zR and its production of Adrenal androgens; a process called adrenarche. The maintenance of the Adrenal Cortex involves the centripetal displacement and differentiation of peripheral Sonic hedgehog-positive progenitors cells into zG cells that later transition to zF cells and subsequently zR cells.
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Recent insights into organogenesis of the Adrenal Cortex.
Trends in endocrinology and metabolism: TEM, 2002Co-Authors: Catherine E. Keegan, Gary D. HammerAbstract:Abstract The primary endocrine organs responsible for steroid hormone biosynthesis – the Adrenal Cortex and gonads – are derived from the urogenital ridge. Several recent discoveries in human and mouse genetics have begun to unravel the complex genetic cascade that dictates adrenocortical cell lineage, proliferation and differentiation. The factors that regulate adrenocortical organogenesis and the maintenance of growth promote or block a cascade of transcription factors that differentially coordinate the proliferation and differentiation of the gland. Here, we outline the developmental milestones of the Adrenal Cortex with recent contributions to the field, focusing on factors that have been shown to play a role in vivo in humans and mice.
Sarah Lambertlanglais – One of the best experts on this subject based on the ideXlab platform.
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a transgenic mouse line with specific cre recombinase expression in the Adrenal Cortex
Molecular and Cellular Endocrinology, 2009Co-Authors: Sarah Lambertlanglais, Pierre Val, Samuel Guyot, Bruno Ragazzon, Isabelle Sahutbarnola, Angelique De Haze, Annemarie Lefrancoismartinez, Antoine MartinezAbstract:Abstract The Cre-loxP system combined with gene targeting strategies has proven to be very useful for gene inactivation in specific tissues and/or cell types. To achieve Adrenal Cortex specific recombination in vivo, we used a 0.5-kb fragment of the 5′-flanking region of the akr1b7 gene to drive Cre expression in adrenocortical cells. The resulting 0.5 akr1b7-Cre mice express Cre in all steroidogenic zones of the Adrenal Cortex but not in the gonads. Although recombination of the ROSA26R reporter locus was not observed in all cortical cells, we provide evidence that Cre is expressed in all the cells of the Cortex in adult mice. In addition, Cre activity was found in collecting ducts and maturing glomeruli of the kidney. This line is the first to show specific Cre expression in the Adrenal Cortex in the absence of Cre expression in the gonads. This transgene thus provides a valuable tool for specific gene recombination in the Adrenal Cortex and kidney.