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Csaba Fekete - One of the best experts on this subject based on the ideXlab platform.
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contribution of norAdrenergic and Adrenergic Cell Groups of the brainstem and agouti related protein synthesizing neurons of the arcuate nucleus to neuropeptide y innervation of corticotropin releasing hormone neurons in hypothalamic paraventricular nucleus of the rat
Endocrinology, 2007Co-Authors: Tamas Fuzesi, Gabor Wittmann, Zsolt Liposits, Ronald M Lechan, Csaba FeketeAbstract:CRH-synthesizing neurons in the hypothalamic paraventricular nucleus (PVN) integrate neuronal and hormonal inputs and serve as a final common pathway to regulate the hypothalamic-pituitary-adrenal axis. One of the neuronal regulators of CRH neurons is neuropeptide Y (NPY) contained in axons that densely innervate CRH neurons. The three main sources of NPY innervation of the PVN are the hypothalamic arcuate nucleus and the norAdrenergic and Adrenergic neurons of the brainstem. To elucidate the origin of the NPY-immunoreactive (NPY-IR) innervation to hypophysiotropic CRH neurons, quadruple-labeling immunocytochemistry for CRH, NPY, dopamine-β-hydroxylase, and phenylethanolamine-N-methyltransferase was performed. Approximately 63% of NPY-IR varicosities on the surface of CRH neurons were catecholaminergic (22% norAdrenergic and 41% Adrenergic), and 37% of NPY-IR boutons were noncatecholaminergic. By triple-labeling immunofluorescence detection of NPY, CRH, and agouti-related protein, a marker of NPY axons pr...
Manabu Negishi - One of the best experts on this subject based on the ideXlab platform.
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prostaglandin ep3 receptor protein in serotonin and catecholamine Cell Groups a double immunofluorescence study in the rat brain
Neuroscience, 2001Co-Authors: Kazuhiro Nakamura, Takeshi Kaneko, Hironori Katoh, Manabu NegishiAbstract:Prostaglandin E2 exerts diverse physiological actions in the central nervous system with unknown mechanisms. We have reported the immunohistochemical localization of the EP3 receptor, one of the prostaglandin E receptor subtypes, in various brain regions including many monoaminergic nuclei. In the present study, a double immunofluorescence technique with an antibody to EP3 receptor and antibodies to markers for monoamine neurons was employed to examine the expression of the receptor in serotonin and catecholamine neurons, and to reveal the distribution of the receptor-expressing monoamine neurons in the rat brain. Almost all serotonergic Cells in the medulla oblongata (B1–B4) exhibited EP3 receptor-like immunoreactivity, whereas mesencephalic and pontine serotonergic Cell Groups (B5–B9) contained relatively small populations of EP3 receptor-immunoreactive Cells. In the catecholaminergic Cell Groups, many of the norAdrenergic A7 Cells in the subcoeruleus nucleus showed immunoreactivity for the receptor. The locus coeruleus exhibited EP3 receptor-like immunoreactivity densely in the neuropil and occasionally in neuronal Cell bodies, all of which were immunopositive for dopamine β-hydroxylase, as observed by confocal laser microscopy. Many of the other norAdrenergic and Adrenergic Cell Groups contained small populations of EP3 receptor-like immunoreactive Cells. In contrast, no EP3 receptor-like immunoreactivity was detected in the norAdrenergic A2 and A4, the Adrenergic C2, and all the dopaminergic Cell Groups. The expression of EP3 receptor by most of the serotonergic, norAdrenergic and Adrenergic Cell Groups suggests that prostaglandin E2 modulates many physiological processes mediated by widely distributed monoaminergic projections through activation of the EP3 receptor on the monoaminergic neurons; for instance, it may modulate nociceptive and autonomic processes by affecting the descending serotonergic pathway from the raphe magnus nucleus to the spinal cord.
Thackery S. Gray - One of the best experts on this subject based on the ideXlab platform.
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Organization of amygdaloid projections to brainstem dopaminergic, norAdrenergic, and Adrenergic Cell Groups in the rat.
Brain research bulletin, 1992Co-Authors: Donna M. Wallace, Debra J. Magnuson, Thackery S. GrayAbstract:The distribution of amygdaloid axons in the various brainstem dopaminergic, norAdrenergic, and Adrenergic Cell Groups was examined. This was accomplished by means of the Phaseolus vulgaris leucoagglutinin lectin (PHA-L) anterograde tracing technique combined with glucose-oxidase immunocytochemistry to catecholamine markers (i.e., tyrosine hydroxylase, dopamine beta hydroxylase, and phenylethanolamine N-methyltransferase). Injections of PHA-L in the medial part of the central amygdaloid nucleus resulted in axonal and terminal labeling in most catecholamine Cell Groups in the brainstem. Amygdaloid terminals appeared to contract catecholaminergic Cells in several brainstem regions. The most heavily innervated catecholaminergic Cells were the A9 (lateral) and A8 dopaminergic Cell Groups and the C2/A2 Adrenergic/norAdrenergic Cell Groups in the nucleus of the solitary tract. The medial part of the A9 and adjacent A10 dopaminergic Cell Groups was moderately innervated. A moderate innervation by amygdaloid terminals was observed on rostral locus coeruleus norAdrenergic Cells (A6 rostral) and Adrenergic Cells of the rostral ventrolateral medulla (C1). NorAdrenergic Cells of the A5, main body of the locus coeruleus (A6), A7, and subcoeruleus were sparsely innervated. Amygdaloid axons were not observed on norAdrenergic neurons of the A4 Cell group, area postrema, and A1 Cells of the ventrolateral medulla. The results demonstrate that the amygdala primarily innervates the dopaminergic Cells of midbrain (i.e., A8 and lateral A9 Cells) and the Adrenergic Cells (C2) and norAdrenergic (A2) Cells in the nucleus of the solitary tract. The possible functional significance of amygdaloid innervation of catecholaminergic Cells is discussed.
Kazuhiro Nakamura - One of the best experts on this subject based on the ideXlab platform.
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prostaglandin ep3 receptor protein in serotonin and catecholamine Cell Groups a double immunofluorescence study in the rat brain
Neuroscience, 2001Co-Authors: Kazuhiro Nakamura, Takeshi Kaneko, Hironori Katoh, Manabu NegishiAbstract:Prostaglandin E2 exerts diverse physiological actions in the central nervous system with unknown mechanisms. We have reported the immunohistochemical localization of the EP3 receptor, one of the prostaglandin E receptor subtypes, in various brain regions including many monoaminergic nuclei. In the present study, a double immunofluorescence technique with an antibody to EP3 receptor and antibodies to markers for monoamine neurons was employed to examine the expression of the receptor in serotonin and catecholamine neurons, and to reveal the distribution of the receptor-expressing monoamine neurons in the rat brain. Almost all serotonergic Cells in the medulla oblongata (B1–B4) exhibited EP3 receptor-like immunoreactivity, whereas mesencephalic and pontine serotonergic Cell Groups (B5–B9) contained relatively small populations of EP3 receptor-immunoreactive Cells. In the catecholaminergic Cell Groups, many of the norAdrenergic A7 Cells in the subcoeruleus nucleus showed immunoreactivity for the receptor. The locus coeruleus exhibited EP3 receptor-like immunoreactivity densely in the neuropil and occasionally in neuronal Cell bodies, all of which were immunopositive for dopamine β-hydroxylase, as observed by confocal laser microscopy. Many of the other norAdrenergic and Adrenergic Cell Groups contained small populations of EP3 receptor-like immunoreactive Cells. In contrast, no EP3 receptor-like immunoreactivity was detected in the norAdrenergic A2 and A4, the Adrenergic C2, and all the dopaminergic Cell Groups. The expression of EP3 receptor by most of the serotonergic, norAdrenergic and Adrenergic Cell Groups suggests that prostaglandin E2 modulates many physiological processes mediated by widely distributed monoaminergic projections through activation of the EP3 receptor on the monoaminergic neurons; for instance, it may modulate nociceptive and autonomic processes by affecting the descending serotonergic pathway from the raphe magnus nucleus to the spinal cord.
Donna M. Wallace - One of the best experts on this subject based on the ideXlab platform.
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Organization of amygdaloid projections to brainstem dopaminergic, norAdrenergic, and Adrenergic Cell Groups in the rat.
Brain research bulletin, 1992Co-Authors: Donna M. Wallace, Debra J. Magnuson, Thackery S. GrayAbstract:The distribution of amygdaloid axons in the various brainstem dopaminergic, norAdrenergic, and Adrenergic Cell Groups was examined. This was accomplished by means of the Phaseolus vulgaris leucoagglutinin lectin (PHA-L) anterograde tracing technique combined with glucose-oxidase immunocytochemistry to catecholamine markers (i.e., tyrosine hydroxylase, dopamine beta hydroxylase, and phenylethanolamine N-methyltransferase). Injections of PHA-L in the medial part of the central amygdaloid nucleus resulted in axonal and terminal labeling in most catecholamine Cell Groups in the brainstem. Amygdaloid terminals appeared to contract catecholaminergic Cells in several brainstem regions. The most heavily innervated catecholaminergic Cells were the A9 (lateral) and A8 dopaminergic Cell Groups and the C2/A2 Adrenergic/norAdrenergic Cell Groups in the nucleus of the solitary tract. The medial part of the A9 and adjacent A10 dopaminergic Cell Groups was moderately innervated. A moderate innervation by amygdaloid terminals was observed on rostral locus coeruleus norAdrenergic Cells (A6 rostral) and Adrenergic Cells of the rostral ventrolateral medulla (C1). NorAdrenergic Cells of the A5, main body of the locus coeruleus (A6), A7, and subcoeruleus were sparsely innervated. Amygdaloid axons were not observed on norAdrenergic neurons of the A4 Cell group, area postrema, and A1 Cells of the ventrolateral medulla. The results demonstrate that the amygdala primarily innervates the dopaminergic Cells of midbrain (i.e., A8 and lateral A9 Cells) and the Adrenergic Cells (C2) and norAdrenergic (A2) Cells in the nucleus of the solitary tract. The possible functional significance of amygdaloid innervation of catecholaminergic Cells is discussed.