The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Jay D Keasling - One of the best experts on this subject based on the ideXlab platform.

  • Engineering terpene biosynthesis in Streptomyces for production of the Advanced Biofuel precursor bisabolene.
    ACS synthetic biology, 2014
    Co-Authors: Ryan M. Phelan, Jay D Keasling, Olga N. Sekurova, Sergey B. Zotchev
    Abstract:

    The past decade has witnessed a large influx of research toward the creation of sustainable, biologically derived fuels. While significant effort has been exerted to improve production capacity in common hosts, such as Escherichia coli or Saccharomyces cerevisiae, studies concerning alternate microbes comparatively lag. In an effort to expand the breadth of characterized hosts for fuel production, we map the terpene biosynthetic pathway in a model actinobacterium, Streptomyces venezuelae, and further alter secondary metabolism to afford the Advanced Biofuel precursor bisabolene. Leveraging information gained from study of the native isoprenoid pathway, we were able to increase bisabolene titer nearly 5-fold over the base production strain, more than 2 orders of magnitude greater than the combined terpene yield in the wild-type host. We also explored production on carbon sources of varying complexity to, notably, define this host as one able to perform consolidated bioprocessing.

  • identification and microbial production of a terpene based Advanced Biofuel
    Nature Communications, 2011
    Co-Authors: Pamela Peraltayahya, Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay
    Abstract:

    Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l(-1). We produce bisabolene in Saccharomyces cerevisiae (>900 mg l(-1)), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into bisabolane. This work presents a framework for the identification of novel terpene-based Advanced Biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of Biofuels.

  • identification and microbial production of a terpene based Advanced Biofuel
    Nature Communications, 2011
    Co-Authors: Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay, Pamela Peraltayahya
    Abstract:

    Advanced Biofuels with comparable properties to petroleum-based fuels could be microbially produced from lignocellulosic biomass. In this study, Escherichia coli is engineered to produce bisabolene, the immediate precursor of bisabolane, a biosynthetic alternative to D2 diesel.

  • Advanced Biofuel production in microbes
    Biotechnology Journal, 2010
    Co-Authors: Pamela Peraltayahya, Jay D Keasling
    Abstract:

    The cost-effective production of Biofuels from renewable materials will begin to address energy security and climate change concerns. Ethanol, naturally produced by microorganisms, is currently the major Biofuel in the transportation sector. However, its low energy content and incompatibility with existing fuel distribution and storage infrastructure limits its economic use in the future. Advanced Biofuels, such as long chain alcohols and isoprenoid- and fatty acid-based Biofuels, have physical properties that more closely resemble petroleum-derived fuels, and as such are an attractive alternative for the future supplementation or replacement of petroleum-derived fuels. Here, we review recent developments in the engineering of metabolic pathways for the production of known and potential Advanced Biofuels by microorganisms. We concentrate on the metabolic engineering of genetically tractable organisms such as Escherichia coli and Saccharomyces cerevisiae for the production of these Advanced Biofuels.

Pamela Peraltayahya - One of the best experts on this subject based on the ideXlab platform.

  • gpcr based chemical biosensors for medium chain fatty acids
    ACS Synthetic Biology, 2015
    Co-Authors: Kuntal Mukherjee, Souryadeep Bhattacharyya, Pamela Peraltayahya
    Abstract:

    A key limitation to engineering microbes for chemical production is a reliance on low-throughput chromatography-based screens for chemical detection. While colorimetric chemicals are amenable to high-throughput screens, many value-added chemicals are not colorimetric and require sensors for high-throughput screening. Here, we use G-protein coupled receptors (GPCRs) known to bind medium-chain fatty acids in mammalian cells to rapidly construct chemical sensors in yeast. Medium-chain fatty acids are immediate precursors to the Advanced Biofuel fatty acid methyl esters, which can serve as a "drop-in" replacement for D2 diesel. One of the sensors detects even-chain C8-C12 fatty acids with a 13- to 17-fold increase in signal after activation, with linear ranges up to 250 μM. Introduction of a synthetic response unit alters both dynamic and linear range, improving the sensor response to decanoic acid to a 30-fold increase in signal after activation, with a linear range up to 500 μM. To our knowledge, this is the first report of a whole-cell medium-chain fatty acid biosensor, which we envision could be applied to the evolutionary engineering of fatty acid-producing microbes. Given the affinity of GPCRs for a wide range of chemicals, it should be possible to rapidly assemble new biosensors by simply swapping the GPCR sensing unit. These sensors should be amenable to a variety of applications that require different dynamic and linear ranges, by introducing different response units.

  • identification and microbial production of a terpene based Advanced Biofuel
    Nature Communications, 2011
    Co-Authors: Pamela Peraltayahya, Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay
    Abstract:

    Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l(-1). We produce bisabolene in Saccharomyces cerevisiae (>900 mg l(-1)), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into bisabolane. This work presents a framework for the identification of novel terpene-based Advanced Biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of Biofuels.

  • identification and microbial production of a terpene based Advanced Biofuel
    Nature Communications, 2011
    Co-Authors: Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay, Pamela Peraltayahya
    Abstract:

    Advanced Biofuels with comparable properties to petroleum-based fuels could be microbially produced from lignocellulosic biomass. In this study, Escherichia coli is engineered to produce bisabolene, the immediate precursor of bisabolane, a biosynthetic alternative to D2 diesel.

  • Advanced Biofuel production in microbes
    Biotechnology Journal, 2010
    Co-Authors: Pamela Peraltayahya, Jay D Keasling
    Abstract:

    The cost-effective production of Biofuels from renewable materials will begin to address energy security and climate change concerns. Ethanol, naturally produced by microorganisms, is currently the major Biofuel in the transportation sector. However, its low energy content and incompatibility with existing fuel distribution and storage infrastructure limits its economic use in the future. Advanced Biofuels, such as long chain alcohols and isoprenoid- and fatty acid-based Biofuels, have physical properties that more closely resemble petroleum-derived fuels, and as such are an attractive alternative for the future supplementation or replacement of petroleum-derived fuels. Here, we review recent developments in the engineering of metabolic pathways for the production of known and potential Advanced Biofuels by microorganisms. We concentrate on the metabolic engineering of genetically tractable organisms such as Escherichia coli and Saccharomyces cerevisiae for the production of these Advanced Biofuels.

Mario Ouellet - One of the best experts on this subject based on the ideXlab platform.

  • identification and microbial production of a terpene based Advanced Biofuel
    Nature Communications, 2011
    Co-Authors: Pamela Peraltayahya, Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay
    Abstract:

    Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l(-1). We produce bisabolene in Saccharomyces cerevisiae (>900 mg l(-1)), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into bisabolane. This work presents a framework for the identification of novel terpene-based Advanced Biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of Biofuels.

  • identification and microbial production of a terpene based Advanced Biofuel
    Nature Communications, 2011
    Co-Authors: Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay, Pamela Peraltayahya
    Abstract:

    Advanced Biofuels with comparable properties to petroleum-based fuels could be microbially produced from lignocellulosic biomass. In this study, Escherichia coli is engineered to produce bisabolene, the immediate precursor of bisabolane, a biosynthetic alternative to D2 diesel.

Aindrila Mukhopadhyay - One of the best experts on this subject based on the ideXlab platform.

  • identification and microbial production of a terpene based Advanced Biofuel
    Nature Communications, 2011
    Co-Authors: Pamela Peraltayahya, Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay
    Abstract:

    Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l(-1). We produce bisabolene in Saccharomyces cerevisiae (>900 mg l(-1)), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into bisabolane. This work presents a framework for the identification of novel terpene-based Advanced Biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of Biofuels.

  • identification and microbial production of a terpene based Advanced Biofuel
    Nature Communications, 2011
    Co-Authors: Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay, Pamela Peraltayahya
    Abstract:

    Advanced Biofuels with comparable properties to petroleum-based fuels could be microbially produced from lignocellulosic biomass. In this study, Escherichia coli is engineered to produce bisabolene, the immediate precursor of bisabolane, a biosynthetic alternative to D2 diesel.

Rossana Chan - One of the best experts on this subject based on the ideXlab platform.

  • identification and microbial production of a terpene based Advanced Biofuel
    Nature Communications, 2011
    Co-Authors: Pamela Peraltayahya, Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay
    Abstract:

    Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l(-1). We produce bisabolene in Saccharomyces cerevisiae (>900 mg l(-1)), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into bisabolane. This work presents a framework for the identification of novel terpene-based Advanced Biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of Biofuels.

  • identification and microbial production of a terpene based Advanced Biofuel
    Nature Communications, 2011
    Co-Authors: Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay, Pamela Peraltayahya
    Abstract:

    Advanced Biofuels with comparable properties to petroleum-based fuels could be microbially produced from lignocellulosic biomass. In this study, Escherichia coli is engineered to produce bisabolene, the immediate precursor of bisabolane, a biosynthetic alternative to D2 diesel.