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Tatjana Boroja – One of the best experts on this subject based on the ideXlab platform.

  • comparative phytochemical analysis of gentiana cruciata l roots and Aerial Parts and their biological activities
    Industrial Crops and Products, 2015
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Sanja Matic, Mirjana Mihailovic, Snežana Stanic, Miroslav M Vrvic, Jelena Katanic, Milan Mladenovic, Nevena Stankovic, Tatjana Boroja

    Abstract:

    Abstract The purpose of this study was to evaluate the antioxidant potential of methanol extracts of Gentiana cruciata L. Aerial Parts and roots, as well as the stability of the phenolic compounds and antioxidant capacity of extracts during heating, at different pHs and after an in vitro digestion procedure. Also, their genotoxicity and antigenotoxicity against carbon tetrachloride in the liver of albino Wistar rats using the comet assay were evaluated. Three secoiridoid glycosides (swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds (orientin, vitexin and two isovitexin-glucosides) were identified as the major constituents in Aerial Parts and roots of G. cruciata , using UHPLC-DAD/±HESI-MS/MS analysis. The results of antioxidant assays showed that Aerial Parts displayed higher antioxidant activity compared to the roots, which could be related to higher phenolics content, especially flavonoids. In general, extracts showed pH and thermal stability, while duodenal condition had more influence on total phenolic condition and antioxidant activity of extracts. Both extracts showed a protective effect against CCl 4 in comet assays. The roots extract showed no genotoxic activity, while Aerial Parts extract showed slight genotoxicity at concentrations of 400 mg/kg b.w.

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C. Eriş – One of the best experts on this subject based on the ideXlab platform.

  • Triterpenoids from the Aerial Parts of Salvia montbretii
    Phytochemistry, 1994
    Co-Authors: Ayhan Ulubelen, Gulacti Topcu, Hermann Lotter, H. Wagner, C. Eriş

    Abstract:

    Abstract From the Aerial Parts of Salvia montbretii , in addition to the known compounds, two new triterpenoids were isolated. The structures of the new compounds were established as 3β- O-cis-p -coumaroylmonogynol A and 3β- O-trans-p -coumaroylmonogynol A by NMR and mass spectroscopy. The structure of monogynol A was confirmed by X-ray diffraction analysis.

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Sri Ramachandra – One of the best experts on this subject based on the ideXlab platform.

  • Aerial Parts of Aerial Parts of Melothria maderaspatana Melothria maderaspatana Melothria maderaspatana Melothria maderaspatana Melothria maderaspatana
    , 2006
    Co-Authors: R. A. Iman, B. Lakshmi Priya, R. Chithra, K Shalini, V. Sharon, D. Chamundeeswari, J Vasantha, Sri Ramachandra

    Abstract:

    Melothria maderaspatana (Linn) Cogn, a plant drug of Siddha medicine, is an annual monoecious tendril climber, belonging to the family Cucurbitaceae, mostly prevalent in South India. It is commonly called Musumusukkai in Tamil. Preliminary phytochemical screening of the plant revealed the presence of phytochemical constituents such as coumarins, flavonoids. Hence an attempt has been made to screen the effect of Melothria maderaspatana in platelet aggregation. Successive extracts of Aerial Parts of Melothria maderaspatana were used in increasing polarity of solvents (i.e., hexane, chloroform, ethyl acetate and methanol). The antiplatelet activity of different fractions of Melothria maderaspatana was studied using platelet-rich plasma in presence of ADP. Each extract was tested in concentrations of 100 µg/ml, 200 µg/ml, 400 µg/ml and 500 µg/ml for the study. The ethyl acetate extract showed a dose-dependent antiplatelet activity. Hexane and methanol extracts showed antiplatelet activity only at 200 µg/ml and 400 µg/ml concentrations. Chloroform extract showed negligible antiplatelet activity. However, the inhibition of platelet aggregation was only 50% when compared to the standard Aspirin. Melothria maderaspatana belongs to the family Cucurbitaceae. appear to inhibit platelet aggregation is by mediating The plant is a tendril climber/prostrate herb 1 . The plant was increase in platelets’ cyclic AMP levels by either reported to have activities such as hepatoprotective 2 , stimulation of adenylate cyclase or inhibition of cAMP antirheumatic 3 , antiflatulent, antiinflammatory, antidiabetic, phosphodiesterase activity 5 . Therefore the experiments in expectorant, diuretic, stomachic, and is used for toothache and this study were designed to evaluate the antiplatelet recommended in vertigo and biliousness 4 . An attempt has activity of various fractions of Melothria maderaspatana been made to study the efficacy of Aerial Parts of Melothria and try to elucidate the inhibitory mechanism of coumarins maderaspatana in platelet aggregation. or flavonoids on platelet aggregation. Blood platelets are involved in haemostasis. The normal The Aerial Parts of Melothria maderaspatana were haemostatic system limits blood loss by precisely regulated collected from the herbal garden of Sri Ramachandra interactions between components of vessel wall, circulating Medical College and Research Institute, Chennai, and blood platelets and plasma proteins. Platelets can adhere to were cleaned, air dried and powdered. The powdered the walls of the blood vessels, release bioreactive Aerial Parts were subjected to exhaustive maceration with compounds and aggregate to each other. These properties increase to a well established level in conditions of arterial thrombosis and atherogenesis. Several agonists such as ADP, thrombin, collagen and serotonin induce the release of arachidonic acid, after phospholipase activation through calcium mobilization 5 . Several drugs have been developed to block the different steps in platelet activation pathways. Inhibition of platelet function by Aspirin has been very well established 6 .

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