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Om Prakash - One of the best experts on this subject based on the ideXlab platform.
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Enhanced disease resistance in the Indian snakehead, Channa punctata against Aeromonas hydrophila, through 5 % feed supplementation with F. benghalensis (Aerial Root) and L. leucocephala (pod seed)
Aquaculture International, 2015Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila , a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila . However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata .
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enhanced disease resistance in the indian snakehead channa punctata against aeromonas hydrophila through 5 feed supplementation with f benghalensis Aerial Root and l leucocephala pod seed
Aquaculture International, 2015Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila, a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila. However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata.
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Enhanced disease resistance in the Indian snakehead, Channa punctata against Aeromonas hydrophila, through 5 % feed supplementation with F. benghalensis (Aerial Root) and L. leucocephala (pod seed)
Aquaculture International, 2014Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila, a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila. However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata.
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immunostimulatory response induced by supplementation of ficus benghalensis Root powder in the artificial feed the indian freshwater murrel channa punctatus
Fish & Shellfish Immunology, 2012Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Abstract Methanol extract from the dried Aerial Root of Ficus benghalensis , was used to evaluate antibacterial activity on the bacterial strains of Aeromonas hydrophila and Escherichia coli , by disc diffusion method. In order to study, if there is any immunostimulatory response of F. benghalensis , immunized fish were fed with supplementary artificial feed containing 5% F. benghalensis dried Root powder. There was no marked difference in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and treated fish, suggesting that the supplementary feed had no adverse effect on liver or kidney. Serum lysozyme, tissue super oxide dismutase (SOD), percentage phagocytosis, phagocytotic index, nitric oxide (NO), total serum protein and immunoglobulin increased significantly in the treated fish compared to control fish. Serum immunoglobulin levels were estimated by development of a sandwich ELISA, and levels were found to increase with successive immunizations of BSA.
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immunostimulatory response induced by supplementation of ficus benghalensis Root powder in the artificial feed the indian freshwater murrel channa punctatus
Fish & Shellfish Immunology, 2012Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Abstract Methanol extract from the dried Aerial Root of Ficus benghalensis , was used to evaluate antibacterial activity on the bacterial strains of Aeromonas hydrophila and Escherichia coli , by disc diffusion method. In order to study, if there is any immunostimulatory response of F. benghalensis , immunized fish were fed with supplementary artificial feed containing 5% F. benghalensis dried Root powder. There was no marked difference in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and treated fish, suggesting that the supplementary feed had no adverse effect on liver or kidney. Serum lysozyme, tissue super oxide dismutase (SOD), percentage phagocytosis, phagocytotic index, nitric oxide (NO), total serum protein and immunoglobulin increased significantly in the treated fish compared to control fish. Serum immunoglobulin levels were estimated by development of a sandwich ELISA, and levels were found to increase with successive immunizations of BSA.
Vipin Kumar Verma - One of the best experts on this subject based on the ideXlab platform.
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Enhanced disease resistance in the Indian snakehead, Channa punctata against Aeromonas hydrophila, through 5 % feed supplementation with F. benghalensis (Aerial Root) and L. leucocephala (pod seed)
Aquaculture International, 2015Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila , a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila . However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata .
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enhanced disease resistance in the indian snakehead channa punctata against aeromonas hydrophila through 5 feed supplementation with f benghalensis Aerial Root and l leucocephala pod seed
Aquaculture International, 2015Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila, a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila. However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata.
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Enhanced disease resistance in the Indian snakehead, Channa punctata against Aeromonas hydrophila, through 5 % feed supplementation with F. benghalensis (Aerial Root) and L. leucocephala (pod seed)
Aquaculture International, 2014Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila, a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila. However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata.
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immunostimulatory response induced by supplementation of ficus benghalensis Root powder in the artificial feed the indian freshwater murrel channa punctatus
Fish & Shellfish Immunology, 2012Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Abstract Methanol extract from the dried Aerial Root of Ficus benghalensis , was used to evaluate antibacterial activity on the bacterial strains of Aeromonas hydrophila and Escherichia coli , by disc diffusion method. In order to study, if there is any immunostimulatory response of F. benghalensis , immunized fish were fed with supplementary artificial feed containing 5% F. benghalensis dried Root powder. There was no marked difference in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and treated fish, suggesting that the supplementary feed had no adverse effect on liver or kidney. Serum lysozyme, tissue super oxide dismutase (SOD), percentage phagocytosis, phagocytotic index, nitric oxide (NO), total serum protein and immunoglobulin increased significantly in the treated fish compared to control fish. Serum immunoglobulin levels were estimated by development of a sandwich ELISA, and levels were found to increase with successive immunizations of BSA.
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immunostimulatory response induced by supplementation of ficus benghalensis Root powder in the artificial feed the indian freshwater murrel channa punctatus
Fish & Shellfish Immunology, 2012Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Abstract Methanol extract from the dried Aerial Root of Ficus benghalensis , was used to evaluate antibacterial activity on the bacterial strains of Aeromonas hydrophila and Escherichia coli , by disc diffusion method. In order to study, if there is any immunostimulatory response of F. benghalensis , immunized fish were fed with supplementary artificial feed containing 5% F. benghalensis dried Root powder. There was no marked difference in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and treated fish, suggesting that the supplementary feed had no adverse effect on liver or kidney. Serum lysozyme, tissue super oxide dismutase (SOD), percentage phagocytosis, phagocytotic index, nitric oxide (NO), total serum protein and immunoglobulin increased significantly in the treated fish compared to control fish. Serum immunoglobulin levels were estimated by development of a sandwich ELISA, and levels were found to increase with successive immunizations of BSA.
Kumari Vandana Rani - One of the best experts on this subject based on the ideXlab platform.
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Enhanced disease resistance in the Indian snakehead, Channa punctata against Aeromonas hydrophila, through 5 % feed supplementation with F. benghalensis (Aerial Root) and L. leucocephala (pod seed)
Aquaculture International, 2015Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila , a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila . However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata .
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enhanced disease resistance in the indian snakehead channa punctata against aeromonas hydrophila through 5 feed supplementation with f benghalensis Aerial Root and l leucocephala pod seed
Aquaculture International, 2015Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila, a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila. However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata.
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Enhanced disease resistance in the Indian snakehead, Channa punctata against Aeromonas hydrophila, through 5 % feed supplementation with F. benghalensis (Aerial Root) and L. leucocephala (pod seed)
Aquaculture International, 2014Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila, a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila. However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata.
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immunostimulatory response induced by supplementation of ficus benghalensis Root powder in the artificial feed the indian freshwater murrel channa punctatus
Fish & Shellfish Immunology, 2012Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Abstract Methanol extract from the dried Aerial Root of Ficus benghalensis , was used to evaluate antibacterial activity on the bacterial strains of Aeromonas hydrophila and Escherichia coli , by disc diffusion method. In order to study, if there is any immunostimulatory response of F. benghalensis , immunized fish were fed with supplementary artificial feed containing 5% F. benghalensis dried Root powder. There was no marked difference in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and treated fish, suggesting that the supplementary feed had no adverse effect on liver or kidney. Serum lysozyme, tissue super oxide dismutase (SOD), percentage phagocytosis, phagocytotic index, nitric oxide (NO), total serum protein and immunoglobulin increased significantly in the treated fish compared to control fish. Serum immunoglobulin levels were estimated by development of a sandwich ELISA, and levels were found to increase with successive immunizations of BSA.
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immunostimulatory response induced by supplementation of ficus benghalensis Root powder in the artificial feed the indian freshwater murrel channa punctatus
Fish & Shellfish Immunology, 2012Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Abstract Methanol extract from the dried Aerial Root of Ficus benghalensis , was used to evaluate antibacterial activity on the bacterial strains of Aeromonas hydrophila and Escherichia coli , by disc diffusion method. In order to study, if there is any immunostimulatory response of F. benghalensis , immunized fish were fed with supplementary artificial feed containing 5% F. benghalensis dried Root powder. There was no marked difference in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and treated fish, suggesting that the supplementary feed had no adverse effect on liver or kidney. Serum lysozyme, tissue super oxide dismutase (SOD), percentage phagocytosis, phagocytotic index, nitric oxide (NO), total serum protein and immunoglobulin increased significantly in the treated fish compared to control fish. Serum immunoglobulin levels were estimated by development of a sandwich ELISA, and levels were found to increase with successive immunizations of BSA.
Neeta Sehgal - One of the best experts on this subject based on the ideXlab platform.
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Enhanced disease resistance in the Indian snakehead, Channa punctata against Aeromonas hydrophila, through 5 % feed supplementation with F. benghalensis (Aerial Root) and L. leucocephala (pod seed)
Aquaculture International, 2015Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila , a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila . However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata .
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enhanced disease resistance in the indian snakehead channa punctata against aeromonas hydrophila through 5 feed supplementation with f benghalensis Aerial Root and l leucocephala pod seed
Aquaculture International, 2015Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila, a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila. However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata.
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Enhanced disease resistance in the Indian snakehead, Channa punctata against Aeromonas hydrophila, through 5 % feed supplementation with F. benghalensis (Aerial Root) and L. leucocephala (pod seed)
Aquaculture International, 2014Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (Aerial Root) or L. leucocephala (pod seeds) 5 % powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila, a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28th day after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila. However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata.
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immunostimulatory response induced by supplementation of ficus benghalensis Root powder in the artificial feed the indian freshwater murrel channa punctatus
Fish & Shellfish Immunology, 2012Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Abstract Methanol extract from the dried Aerial Root of Ficus benghalensis , was used to evaluate antibacterial activity on the bacterial strains of Aeromonas hydrophila and Escherichia coli , by disc diffusion method. In order to study, if there is any immunostimulatory response of F. benghalensis , immunized fish were fed with supplementary artificial feed containing 5% F. benghalensis dried Root powder. There was no marked difference in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and treated fish, suggesting that the supplementary feed had no adverse effect on liver or kidney. Serum lysozyme, tissue super oxide dismutase (SOD), percentage phagocytosis, phagocytotic index, nitric oxide (NO), total serum protein and immunoglobulin increased significantly in the treated fish compared to control fish. Serum immunoglobulin levels were estimated by development of a sandwich ELISA, and levels were found to increase with successive immunizations of BSA.
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immunostimulatory response induced by supplementation of ficus benghalensis Root powder in the artificial feed the indian freshwater murrel channa punctatus
Fish & Shellfish Immunology, 2012Co-Authors: Vipin Kumar Verma, Kumari Vandana Rani, Neeta Sehgal, Om PrakashAbstract:Abstract Methanol extract from the dried Aerial Root of Ficus benghalensis , was used to evaluate antibacterial activity on the bacterial strains of Aeromonas hydrophila and Escherichia coli , by disc diffusion method. In order to study, if there is any immunostimulatory response of F. benghalensis , immunized fish were fed with supplementary artificial feed containing 5% F. benghalensis dried Root powder. There was no marked difference in the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in control and treated fish, suggesting that the supplementary feed had no adverse effect on liver or kidney. Serum lysozyme, tissue super oxide dismutase (SOD), percentage phagocytosis, phagocytotic index, nitric oxide (NO), total serum protein and immunoglobulin increased significantly in the treated fish compared to control fish. Serum immunoglobulin levels were estimated by development of a sandwich ELISA, and levels were found to increase with successive immunizations of BSA.
André Mantovani - One of the best experts on this subject based on the ideXlab platform.
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Aerial Root hydraulic conductivity increases with plant size for the aroid vine Rhodospatha oblongata (Araceae)
Journal of Plant Hydraulics, 2018Co-Authors: Arinawa Liz Filartiga, Ricardo Cardoso Vieira, André MantovaniAbstract:Rhodospatha oblongata (Araceae) is an aroid vine which reaches maturity at trees canopies. The beginning of R. oblongata’s ascension towards the canopy occurs when one of the branches reaches the stem of a host, being able to reach eight to ten meters in height. Throughout this ascendant path R. oblongata develops two types of Aerial Roots: the anchor Roots, which is shorter and adhered to the host, never reaching the soil; and the feeder Roots, which is long, also adheres to the host but connects the vine to the forest soil. Both Roots are here compared in morpho-physiological aspects related to the efficiency of axial hydraulic conductivity. Two hypotheses are tested: i) both Roots present distinct xylem hydraulic conductivity; ii) hydraulic conductivity of both Roots vary with plant size. The characterization of the Roots was based on crescent R. oblongata individuals divided in five size classes. Thirty specimens of each anchor and feeder Roots were analyzed along plant size increase. Both Roots gradually increase in number and external diameter while the R. oblongata vertically ascends to reach plant canopies. The stele of both Roots increase in diameter, in order to accommodate xylem vessels that became larger. The increase in these morpho-anatomical parameters has a positive influence on the xylem hydraulic conductivity, that also increases along the ascendant way of R. oblongata. Comparative measurements show that in general anchor Roots present smaller morpho-anatomical structures and lower hydraulic conductivity in comparison to feeder Roots. Xylem diameter distribution is unimodal for anchor Roots, but bimodal for feeder ones. While all feeder Roots present a great concentration of vessels around 60 mm of diameter, the second peak occurs at xylem diameter values that increase with plant size. These modifications optimize the Root water transport while the vegetative body of R. oblongata increases in size, connecting its leaves at canopies to the soil water with elevated hydraulic efficiency
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Aerial Root hydraulic conductivity increases with plant size for the aroid vine Rhodospatha oblongata (Araceae)
Journal of Plant Hydraulics, 2017Co-Authors: Arinawa Liz Filartiga, Ricardo Vieira, André MantovaniAbstract:Rhodospatha oblongata (Araceae) is an aroid vine which reaches maturity at tree canopies. The beginning of its ascension towards the canopy occurs when one of the branches reaches the stem of a host, thenceforward reaching eight to ten meters in height. Throughout this ascendant path, R. oblongata develops two types of Aerial Roots: anchor Roots, which are shorter and adhere to the host, never reaching the soil, and feeder Roots, which are longer and also adhere to the host, but connect the vine to the forest soil. The morphological, anatomical and physiological aspects of both Root types are herein compared in relation to the efficiency of axial hydraulic conductivity. It is hypothesized that i) the two Root types present distinct xylem hydraulic conductivity and ii) hydraulic conductivity of both Roots varies with plant size. Root characterization was based on growing R. oblongata individuals divided into five size classes. Thirty specimens with both anchor and feeder Roots were analyzed along plant size increase. Both Roots gradually increase in number and external diameter as R. oblongata vertically ascends to reach plant canopies. The stele of both Roots increases in diameter, as well as their respective xylem vessels that become larger. The increase in these morphological and anatomical parameters has a positive influence on xylem hydraulic conductivity, which also increases along the ascendance of R. oblongata. Comparative measurements show that anchor Roots, in general, present smaller morphological and anatomical structures and lower hydraulic conductivity in comparison to feeder Roots. Xylem diameter distribution is unimodal for anchor Roots, but bimodal for feeder Roots. While all feeder Roots present a great concentration of vessels around 60 µm in diameter, the second peak occurs at xylem diameter values that increase with plant size. These modifications optimize Root water transport, while the vegetative body of R. oblongata increases in size, connecting its leaves at canopies to the soil water with elevated hydraulic efficiency.
