The Experts below are selected from a list of 69 Experts worldwide ranked by ideXlab platform
Fanaei Hamed - One of the best experts on this subject based on the ideXlab platform.
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Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying
2017Co-Authors: Seyedabadi Mohammad, Ramezani Vahid, Vatanara Alireza, Nabi Meybodi Mohsen, Fanaei HamedAbstract:Objectives: Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray dried IgG. Methods: D-optimal design was employed for experimental design as well as analysis and optimization of the variables. The size and Aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively. In addition, stability, ratio of beta sheets, and morphology of antibody were analyzed using size exclusion chromatography, IR spectroscopy, and electron microscopy, respectively. Results: Particle properties and antibody stability were significantly improved in the presence of HPBCD. In addition, particle Aerodynamic behavior, in terms of fine particle fraction (FPF), enhanced up to 52.23%. Furthermore, antibody was better preserved not only during spray drying, but also during long term storage. In contrast, application of BCD resulted in the formation of larger particles. Although trehalose caused inappropriate Aerodynamic Property, it efficiently decreased antibody aggregation. Conclusion: HPBCD is an efficient excipient for development of inhalable protein formulations. In this regard, optimal particle Property and antibody stability was obtained with proper combination of cyclodextrins and simple sugars, such as trehalose
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Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying
2017Co-Authors: Seyedabadi Mohammad, Ramezani Vahid, Vatanara Alireza, Nabi-meibodi Mohsen, Fanaei HamedAbstract:Objectives: Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray-dried IgG. Methods: D-optimal design was employed for both experimental design and analysis and optimization of the variables. The size and Aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively. In addition, stability, ratio of beta sheets and morphology of antibody were analyzed using size exclusion chromatography, IR spectroscopy and electron microscopy, respectively. Results: Particle properties and antibody stability were significantly improved in the presence of HPBCD. In addition, particle Aerodynamic behavior, in terms of fine-particle fraction (FPF), enhanced up to 52.23%. Furthermore, antibody was better preserved not only during spray drying, but also during long-term storage. In contrast, application of BCD resulted in the formation of larger particles. Although trehalose caused inappropriate Aerodynamic Property, it efficiently decreased antibody aggregation. Conclusion: HPBCD is an efficient excipient for the development of inhalable protein formulations. In this regard, optimal particle Property and antibody stability was obtained with proper combination of cyclodextrins and simple sugars, such as trehalose
Gantasala Sudhakar - One of the best experts on this subject based on the ideXlab platform.
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Numerical Investigation of the Aeroelastic Behavior of a Wind Turbine with Iced Blades
2019Co-Authors: Gantasala Sudhakar, Tabatabaei Narges, Cervantes Michel, Aidanpää Jan-olovAbstract:Wind turbines installed in cold-climate regions are prone to the risks of ice accumulation which affects their aeroelastic behavior. The studies carried out on this topic so far considered icing in a few sections of the blade, mostly located in the outer part of the blade, and their influence on the loads and power production of the turbine are only analyzed. The knowledge about the influence of icing in different locations of the blade and asymmetrical icing of the blades on loads, power, and vibration behavior of the turbine is still not matured. To improve this knowledge, multiple simulation cases are needed to run with different ice accumulations on the blade considering structural and Aerodynamic Property changes due to ice. Such simulations can be easily run by automating the ice shape creation on aerofoil sections and two-dimensional (2-D) Computational Fluid Dynamics (CFD) analysis of those sections. The current work proposes such methodology and it is illustrated on the National Renewable Energy Laboratory (NREL) 5 MW baseline wind turbine model. The influence of symmetrical icing in different locations of the blade and asymmetrical icing of the blade assembly is analyzed on the turbine’s dynamic behavior using the aeroelastic computer-aided engineering tool FAST. The outer third of the blade produces about 50% of the turbine’s total power and severe icing in this part of the blade reduces power output and aeroelastic damping of the blade’s flapwise vibration modes. The increase in blade mass due to ice reduces its natural frequencies which can be extracted from the vibration responses of the turbine operating under turbulent wind conditions. Symmetrical icing of the blades reduces loads acting on the turbine components, whereas asymmetrical icing of the blades induces loads and vibrations in the tower, hub, and nacelle assembly at a frequency synchronous to rotational speed of the turbine.Validerad;2019;Nivå 2;2019-07-05 (johcin)
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Detection of blade icing and its influence on wind turbine vibrations
2019Co-Authors: Gantasala SudhakarAbstract:Wind turbine installations in extreme conditions like cold climate have increased over thelast few years and expected to grow in future in North America, Europe, and Asia regions due to good wind resources and land availability. Their installed capacity could reach 186 GW by the end of 2020. The cold climate sites impose the risk of ice accumulation on turbines during the winter due to the humidity at low temperatures. Since the atmospheric and operating conditions of the wind turbine leading to blade icing vary stochastically in space and time, the resulting ice accumulation is completely random, it is even different for turbines within the same site. Ice accumulation alters aerofoil shapes of the blade, affecting their aeroelastic behavior. The icing severity at different locations of the blade and their non-uniform distribution on blades have a distinct influence on turbine power output and vibrations. The current thesis proposes a methodology to investigate such behavior of wind turbines by considering the structural and Aerodynamic Property changes in the blade due to icing. An automated procedure is used to scale simulated/measured ice shape on aerofoil sections of the blade according to a specified ice mass distribution. The aeroelastic behavior of the blades is simulated considering the static Aerodynamic coefficients of the iced aerofoil sections. The proposed methodology is demonstrated on the National Renewable Energy Laboratory (NREL) 5 MW baseline wind turbine model. The method can be leveraged to analyze the influence of icing on any wind turbine model. De/Anti-icing systems are installed on the turbines to mitigate the risks associated with icing. It is essential to detect icing at the early stage and initiate these systems to avoid production losses and limit the risks associated with ice throw. Ice accumulation increases blade mass and its spatial distribution changes natural frequencies of the blade. A detection technique is proposed in this thesis to characterize ice mass distribution on the blades based on its natural frequencies. The detection technique is validated using experiments on a small-scale cantilever beam and 1-kW wind turbine blade set-ups and its effectiveness is also verified on large-scale wind turbine blades using numerical models. The proposed technique has the potential for detecting ice masses on large wind turbines operating in cold climate as it requires only first few natural frequencies of the blade. These natural frequencies are usually excited by the turbulent wind in operation/standstill conditions and they can be estimated from the vibration measurements of the blade
Seyedabadi Mohammad - One of the best experts on this subject based on the ideXlab platform.
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Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying
2017Co-Authors: Seyedabadi Mohammad, Ramezani Vahid, Vatanara Alireza, Nabi Meybodi Mohsen, Fanaei HamedAbstract:Objectives: Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray dried IgG. Methods: D-optimal design was employed for experimental design as well as analysis and optimization of the variables. The size and Aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively. In addition, stability, ratio of beta sheets, and morphology of antibody were analyzed using size exclusion chromatography, IR spectroscopy, and electron microscopy, respectively. Results: Particle properties and antibody stability were significantly improved in the presence of HPBCD. In addition, particle Aerodynamic behavior, in terms of fine particle fraction (FPF), enhanced up to 52.23%. Furthermore, antibody was better preserved not only during spray drying, but also during long term storage. In contrast, application of BCD resulted in the formation of larger particles. Although trehalose caused inappropriate Aerodynamic Property, it efficiently decreased antibody aggregation. Conclusion: HPBCD is an efficient excipient for development of inhalable protein formulations. In this regard, optimal particle Property and antibody stability was obtained with proper combination of cyclodextrins and simple sugars, such as trehalose
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Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying
2017Co-Authors: Seyedabadi Mohammad, Ramezani Vahid, Vatanara Alireza, Nabi-meibodi Mohsen, Fanaei HamedAbstract:Objectives: Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray-dried IgG. Methods: D-optimal design was employed for both experimental design and analysis and optimization of the variables. The size and Aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively. In addition, stability, ratio of beta sheets and morphology of antibody were analyzed using size exclusion chromatography, IR spectroscopy and electron microscopy, respectively. Results: Particle properties and antibody stability were significantly improved in the presence of HPBCD. In addition, particle Aerodynamic behavior, in terms of fine-particle fraction (FPF), enhanced up to 52.23%. Furthermore, antibody was better preserved not only during spray drying, but also during long-term storage. In contrast, application of BCD resulted in the formation of larger particles. Although trehalose caused inappropriate Aerodynamic Property, it efficiently decreased antibody aggregation. Conclusion: HPBCD is an efficient excipient for the development of inhalable protein formulations. In this regard, optimal particle Property and antibody stability was obtained with proper combination of cyclodextrins and simple sugars, such as trehalose
Ramezani Vahid - One of the best experts on this subject based on the ideXlab platform.
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Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying
2017Co-Authors: Seyedabadi Mohammad, Ramezani Vahid, Vatanara Alireza, Nabi Meybodi Mohsen, Fanaei HamedAbstract:Objectives: Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray dried IgG. Methods: D-optimal design was employed for experimental design as well as analysis and optimization of the variables. The size and Aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively. In addition, stability, ratio of beta sheets, and morphology of antibody were analyzed using size exclusion chromatography, IR spectroscopy, and electron microscopy, respectively. Results: Particle properties and antibody stability were significantly improved in the presence of HPBCD. In addition, particle Aerodynamic behavior, in terms of fine particle fraction (FPF), enhanced up to 52.23%. Furthermore, antibody was better preserved not only during spray drying, but also during long term storage. In contrast, application of BCD resulted in the formation of larger particles. Although trehalose caused inappropriate Aerodynamic Property, it efficiently decreased antibody aggregation. Conclusion: HPBCD is an efficient excipient for development of inhalable protein formulations. In this regard, optimal particle Property and antibody stability was obtained with proper combination of cyclodextrins and simple sugars, such as trehalose
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Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying
2017Co-Authors: Seyedabadi Mohammad, Ramezani Vahid, Vatanara Alireza, Nabi-meibodi Mohsen, Fanaei HamedAbstract:Objectives: Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray-dried IgG. Methods: D-optimal design was employed for both experimental design and analysis and optimization of the variables. The size and Aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively. In addition, stability, ratio of beta sheets and morphology of antibody were analyzed using size exclusion chromatography, IR spectroscopy and electron microscopy, respectively. Results: Particle properties and antibody stability were significantly improved in the presence of HPBCD. In addition, particle Aerodynamic behavior, in terms of fine-particle fraction (FPF), enhanced up to 52.23%. Furthermore, antibody was better preserved not only during spray drying, but also during long-term storage. In contrast, application of BCD resulted in the formation of larger particles. Although trehalose caused inappropriate Aerodynamic Property, it efficiently decreased antibody aggregation. Conclusion: HPBCD is an efficient excipient for the development of inhalable protein formulations. In this regard, optimal particle Property and antibody stability was obtained with proper combination of cyclodextrins and simple sugars, such as trehalose
Vatanara Alireza - One of the best experts on this subject based on the ideXlab platform.
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Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying
2017Co-Authors: Seyedabadi Mohammad, Ramezani Vahid, Vatanara Alireza, Nabi Meybodi Mohsen, Fanaei HamedAbstract:Objectives: Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray dried IgG. Methods: D-optimal design was employed for experimental design as well as analysis and optimization of the variables. The size and Aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively. In addition, stability, ratio of beta sheets, and morphology of antibody were analyzed using size exclusion chromatography, IR spectroscopy, and electron microscopy, respectively. Results: Particle properties and antibody stability were significantly improved in the presence of HPBCD. In addition, particle Aerodynamic behavior, in terms of fine particle fraction (FPF), enhanced up to 52.23%. Furthermore, antibody was better preserved not only during spray drying, but also during long term storage. In contrast, application of BCD resulted in the formation of larger particles. Although trehalose caused inappropriate Aerodynamic Property, it efficiently decreased antibody aggregation. Conclusion: HPBCD is an efficient excipient for development of inhalable protein formulations. In this regard, optimal particle Property and antibody stability was obtained with proper combination of cyclodextrins and simple sugars, such as trehalose
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Application of cyclodextrins in antibody microparticles: potentials for antibody protection in spray drying
2017Co-Authors: Seyedabadi Mohammad, Ramezani Vahid, Vatanara Alireza, Nabi-meibodi Mohsen, Fanaei HamedAbstract:Objectives: Dry powder formulations are extensively used to improve the stability of antibodies. Spray drying is one of important methods for protein drying. This study investigated the effects of trehalose, hydroxypropyl beta cyclodextrin (HPBCD) and beta cyclodextrin (BCD) on the stability and particle properties of spray-dried IgG. Methods: D-optimal design was employed for both experimental design and analysis and optimization of the variables. The size and Aerodynamic behavior of particles were determined using laser light scattering and glass twin impinger, respectively. In addition, stability, ratio of beta sheets and morphology of antibody were analyzed using size exclusion chromatography, IR spectroscopy and electron microscopy, respectively. Results: Particle properties and antibody stability were significantly improved in the presence of HPBCD. In addition, particle Aerodynamic behavior, in terms of fine-particle fraction (FPF), enhanced up to 52.23%. Furthermore, antibody was better preserved not only during spray drying, but also during long-term storage. In contrast, application of BCD resulted in the formation of larger particles. Although trehalose caused inappropriate Aerodynamic Property, it efficiently decreased antibody aggregation. Conclusion: HPBCD is an efficient excipient for the development of inhalable protein formulations. In this regard, optimal particle Property and antibody stability was obtained with proper combination of cyclodextrins and simple sugars, such as trehalose
