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AFF1
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Qiang Zhou – One of the best experts on this subject based on the ideXlab platform.
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Structural basis for ELL2 and AFF4 activation of HIV-1 proviral transcription.
Nature communications, 2017Co-Authors: Ursula Schulze-gahmen, Qiang Zhou, Goran Stjepanovic, James H. HurleyAbstract:The intrinsically disordered scaffold proteins AFF1/4 and the transcription elongation factors ELL1/2 are core components of the super elongation complex required for HIV-1 proviral transcription. Here we report the 2.0-A resolution crystal structure of the human ELL2 C-terminal domain bound to its 50-residue binding site on AFF4, the ELLBow. The ELL2 domain has the same arch-shaped fold as the tight junction protein occludin. The ELLBow consists of an N-terminal helix followed by an extended hairpin that we refer to as the elbow joint, and occupies most of the concave surface of ELL2. This surface is important for the ability of ELL2 to promote HIV-1 Tat-mediated proviral transcription. The AFF4-ELL2 interface is imperfectly packed, leaving a cavity suggestive of a potential binding site for transcription-promoting small molecules.
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How the AFF1/4 scaffold recruits the elongation factor ELL2 to promote HIV-1 proviral transcription
, 2016Co-Authors: Ursula Schulze-gahmen, Qiang Zhou, Goran Stjepanovic, James H. HurleyAbstract:The intrinsically disordered scaffold proteins AFF1/4 and the transcription elongation factors ELL1/2 are core components of the superelongation complex required for HIV-1 proviral transcription. We determined the 2.0-A resolution crystal structure of the human ELL2 C-terminal domain bound to its 50-residue binding site on AFF4, the ELLBow. The ELL2 domain has the same arch-shaped fold as the tight junction protein occludin. The ELLBow consists of an N-terminal helix followed by an extended hairpin that we refer to as the elbow joint, and occupies most of the concave surface of ELL2. This surface is important for the ability of ELL2 to promote HIV-1 Tat-mediated proviral transcription. The AFF4-ELL2 interface is imperfectly packed, leaving a cavity suggestive of a potential binding site for transcription-promoting small molecules.
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how the AFF1 4 scaffold recruits the elongation factor ell2 to promote hiv 1 proviral transcription
bioRxiv, 2016Co-Authors: Ursula Schulzegahmen, Qiang Zhou, Goran Stjepanovic, James H. HurleyAbstract:The intrinsically disordered scaffold proteins AFF1/4 and the transcription elongation factors ELL1/2 are core components of the superelongation complex required for HIV-1 proviral transcription. We determined the 2.0-A resolution crystal structure of the human ELL2 C-terminal domain bound to its 50-residue binding site on AFF4, the ELLBow. The ELL2 domain has the same arch-shaped fold as the tight junction protein occludin. The ELLBow consists of an N-terminal helix followed by an extended hairpin that we refer to as the elbow joint, and occupies most of the concave surface of ELL2. This surface is important for the ability of ELL2 to promote HIV-1 Tat-mediated proviral transcription. The AFF4-ELL2 interface is imperfectly packed, leaving a cavity suggestive of a potential binding site for transcription-promoting small molecules.
Y. Yogo – One of the best experts on this subject based on the ideXlab platform.
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JC virus genotypes in a Taiwan aboriginal tribe (Bunun): implications for its population history^*
Archives of Virology, 1999Co-Authors: D. Chang, C. Sugimoto, M. Wang, R.-t. Tsai, Y. YogoAbstract:The origin of Taiwanese aborigines remains obscure; it has been speculated that they may be from either mainland China or southeastern Asia. We used the JCV genotyping method to elucidate the origin of Bunun aborigines who now live in central mountain areas of Taiwan. We found that Bunun aborigines carried two major (B1-Aff2 and CY) and two minor JCV genotypes (B1-AFF1 and SC). This was contrasted with the JCV genotype profile in modern Taiwanese: one major (SC) and two minor genotypes (CY and B1-AFF1). It thus appears that B1-Aff2 and CY are indigenous to the Bunun tribe. B1-Aff2 was first identified in this study as a discrete cluster that contained only Bunun and Philippine JCV isolates and that was closely related to B1-AFF1, one of the three common JCV genotypes in China. CY predominates in North China, while SC predominates in South China and southeastern Asia. The present findings suggest that the Bunun tribe is an admixture of two ethnic groups, one carrying B1-Aff2 and the other carrying CY. In other words, it is likely that the Bunun tribe was established by two waves of immigrations from mainland Asia, predating those by southern Chinese which began in the 17th century.
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JC virus genotypes in a Taiwan aboriginal tribe (Bunun): implications for its population history *
Archives of virology, 1999Co-Authors: D. Chang, C. Sugimoto, M. Wang, R.-t. Tsai, Y. YogoAbstract:The origin of Taiwanese aborigines remains obscure; it has been speculated that they may be from either mainland China or southeastern Asia. We used the JCV genotyping method to elucidate the origin of Bunun aborigines who now live in central mountain areas of Taiwan. We found that Bunun aborigines carried two major (B1-Aff2 and CY) and two minor JCV genotypes (B1-AFF1 and SC). This was contrasted with the JCV genotype profile in modern Taiwanese: one major (SC) and two minor genotypes (CY and B1-AFF1). It thus appears that B1-Aff2 and CY are indigenous to the Bunun tribe. B1-Aff2 was first identified in this study as a discrete cluster that contained only Bunun and Philippine JCV isolates and that was closely related to B1-AFF1, one of the three common JCV genotypes in China. CY predominates in North China, while SC predominates in South China and southeastern Asia. The present findings suggest that the Bunun tribe is an admixture of two ethnic groups, one carrying B1-Aff2 and the other carrying CY. In other words, it is likely that the Bunun tribe was established by two waves of immigrations from mainland Asia, predating those by southern Chinese which began in the 17th century.
D. Chang – One of the best experts on this subject based on the ideXlab platform.
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JC virus genotypes in a Taiwan aboriginal tribe (Bunun): implications for its population history^*
Archives of Virology, 1999Co-Authors: D. Chang, C. Sugimoto, M. Wang, R.-t. Tsai, Y. YogoAbstract:The origin of Taiwanese aborigines remains obscure; it has been speculated that they may be from either mainland China or southeastern Asia. We used the JCV genotyping method to elucidate the origin of Bunun aborigines who now live in central mountain areas of Taiwan. We found that Bunun aborigines carried two major (B1-Aff2 and CY) and two minor JCV genotypes (B1-AFF1 and SC). This was contrasted with the JCV genotype profile in modern Taiwanese: one major (SC) and two minor genotypes (CY and B1-AFF1). It thus appears that B1-Aff2 and CY are indigenous to the Bunun tribe. B1-Aff2 was first identified in this study as a discrete cluster that contained only Bunun and Philippine JCV isolates and that was closely related to B1-AFF1, one of the three common JCV genotypes in China. CY predominates in North China, while SC predominates in South China and southeastern Asia. The present findings suggest that the Bunun tribe is an admixture of two ethnic groups, one carrying B1-Aff2 and the other carrying CY. In other words, it is likely that the Bunun tribe was established by two waves of immigrations from mainland Asia, predating those by southern Chinese which began in the 17th century.
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JC virus genotypes in a Taiwan aboriginal tribe (Bunun): implications for its population history *
Archives of virology, 1999Co-Authors: D. Chang, C. Sugimoto, M. Wang, R.-t. Tsai, Y. YogoAbstract:The origin of Taiwanese aborigines remains obscure; it has been speculated that they may be from either mainland China or southeastern Asia. We used the JCV genotyping method to elucidate the origin of Bunun aborigines who now live in central mountain areas of Taiwan. We found that Bunun aborigines carried two major (B1-Aff2 and CY) and two minor JCV genotypes (B1-AFF1 and SC). This was contrasted with the JCV genotype profile in modern Taiwanese: one major (SC) and two minor genotypes (CY and B1-AFF1). It thus appears that B1-Aff2 and CY are indigenous to the Bunun tribe. B1-Aff2 was first identified in this study as a discrete cluster that contained only Bunun and Philippine JCV isolates and that was closely related to B1-AFF1, one of the three common JCV genotypes in China. CY predominates in North China, while SC predominates in South China and southeastern Asia. The present findings suggest that the Bunun tribe is an admixture of two ethnic groups, one carrying B1-Aff2 and the other carrying CY. In other words, it is likely that the Bunun tribe was established by two waves of immigrations from mainland Asia, predating those by southern Chinese which began in the 17th century.