The Experts below are selected from a list of 45552 Experts worldwide ranked by ideXlab platform
Bangding Xiao - One of the best experts on this subject based on the ideXlab platform.
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efficacy of zero nitrous oxide emitting aerobic denitrifying bacterium methylobacterium gregans dc 1 in nitRate removal with strong auto Aggregation property
Bioresource Technology, 2019Co-Authors: Pei Hong, Yilin Shu, Chunbo Wang, Cuicui Tian, Oscar Omondi Donde, Bangding XiaoAbstract:Abstract A novel aerobic denitrifying strain Methylobacterium gregans DC-1 was isolated and identified. Strain DC-1 removed 98.4% of nitRate-nitrogen (NO3−-N) and 80.7% of total organic carbon with initial concentrations of 50 and 2400 mg/l, respectively. The N balance showed that most NO3−-N was converted to N2 (62.18%) without nitrous oxide (N2O) emission. Response surface analysis showed that the optimal conditions for total N removal were carbon (C):N ratio of 18.7, temperature of 26.8 °C, pH of 6.5 and shaking speed of 180 rpm. In combination with the N balance and successful amplification of napA, nirK and nosZ genes, the metabolic pathway was as follows: NO3−NO2− → NO → N2O → N2. Strain DC-1 had strong auto-Aggregation Rate (maximum 38.7%), produced large amounts of extracellular polymeric substances (EPS; maximum of 781.4 mg/g cell dry weight) and had corresponding strong hydrophobicity (maximum 83.2%). Pearson correlation analysis showed that EPS content and hydrophobicity were significantly positively correlated with auto-Aggregation.
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efficacy of zero nitrous oxide emitting aerobic denitrifying bacterium methylobacterium gregans dc 1 in nitRate removal with strong auto Aggregation property
Bioresource Technology, 2019Co-Authors: Pei Hong, Yilin Shu, Chunbo Wang, Cuicui Tian, Oscar Omondi Donde, Bangding XiaoAbstract:A novel aerobic denitrifying strain Methylobacterium gregans DC-1 was isolated and identified. Strain DC-1 removed 98.4% of nitRate-nitrogen (NO3--N) and 80.7% of total organic carbon with initial concentrations of 50 and 2400 mg/1, respectively. The N balance showed that most NO3--N was converted to N-2(62.18%) without nitrous oxide (N2O) emission. Response surface analysis showed that the optimal conditions for total N removal were carbon (C):N ratio of 18.7, temperature of 26.8 degrees C, pH of 6.5 and shaking speed of 180 rpm. In combination with the N balance and successful amplification of napA, nirK and nosZ genes, the metabolic pathway was as follows: NO3-NO2- -> NO -> N-2(O) -> N-2. Strain DC-1 had strong auto-Aggregation Rate (maximum 38.7%), produced large amounts of extracellular polymeric substances (EPS; maximum of 781.4 mg/g cell dry weight) and had corresponding strong hydrophobicity (maximum 83.2%). Pearson correlation analysis showed that EPS content and hydrophobicity were significantly positively correlated with auto-Aggregation.
Theodore W Randolph - One of the best experts on this subject based on the ideXlab platform.
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partial molar volume surface area and hydration changes for equilibrium unfolding and formation of Aggregation transition state high pressure and cosolute studies on recombinant human ifn γ
Proceedings of the National Academy of Sciences of the United States of America, 2001Co-Authors: Jonathan N Webb, Serena D Webb, Jeffrey L Cleland, John F Carpenter, Theodore W RandolphAbstract:The equilibrium dissociation of recombinant human IFN-γ was monitored as a function of pressure and sucrose concentration. The partial molar volume change for dissociation was −209 ± 13 ml/mol of dimer. The specific molar surface area change for dissociation was 12.7 ± 1.6 nm2/molecule of dimer. The first-order Aggregation Rate of recombinant human IFN-γ in 0.45 M guanidine hydrochloride was studied as a function of sucrose concentration and pressure. Aggregation proceeded through a transition-state species, N*. Sucrose reduced Aggregation Rate by shifting the equilibrium between native state (N) and N* toward the more compact N. Pressure increased Aggregation Rate through increased solvation of the protein, which exposes more surface area, thus shifting the equilibrium away from N toward N*. The changes in partial molar volume and specific molar surface area between the N* and N were −41 ± 9 ml/mol of dimer and 3.5 ± 0.2 nm2/molecule, respectively. Thus, the structural change required for the formation of the transition state for Aggregation is small relative to the difference between N and the dissociated state. Changes in waters of hydration were estimated from both specific molar surface area and partial molar volume data. From partial molar volume data, estimates were 25 and 128 mol H2O/mol dimer for formation of the Aggregation transition state and for dissociation, respectively. From surface area data, estimates were 27 and 98 mol H2O/mol dimer. Osmotic stress theory yielded values ≈4-fold larger for both transitions.
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partial molar volume surface area and hydration changes for equilibrium unfolding and formation of Aggregation transition state high pressure and cosolute studies on recombinant human ifn γ
Proceedings of the National Academy of Sciences of the United States of America, 2001Co-Authors: Jonathan N Webb, Serena D Webb, Jeffrey L Cleland, John F Carpenter, Theodore W RandolphAbstract:The equilibrium dissociation of recombinant human IFN-gamma was monitored as a function of pressure and sucrose concentration. The partial molar volume change for dissociation was -209 +/- 13 ml/mol of dimer. The specific molar surface area change for dissociation was 12.7 +/- 1.6 nm2/molecule of dimer. The first-order Aggregation Rate of recombinant human IFN-gamma in 0.45 M guanidine hydrochloride was studied as a function of sucrose concentration and pressure. Aggregation proceeded through a transition-state species, N*. Sucrose reduced Aggregation Rate by shifting the equilibrium between native state (N) and N* toward the more compact N. Pressure increased Aggregation Rate through increased solvation of the protein, which exposes more surface area, thus shifting the equilibrium away from N toward N*. The changes in partial molar volume and specific molar surface area between the N* and N were -41 +/- 9 ml/mol of dimer and 3.5 +/- 0.2 nm2/molecule, respectively. Thus, the structural change required for the formation of the transition state for Aggregation is small relative to the difference between N and the dissociated state. Changes in waters of hydration were estimated from both specific molar surface area and partial molar volume data. From partial molar volume data, estimates were 25 and 128 mol H2O/mol dimer for formation of the Aggregation transition state and for dissociation, respectively. From surface area data, estimates were 27 and 98 mol H2O/mol dimer. Osmotic stress theory yielded values approximately 4-fold larger for both transitions.
Pei Hong - One of the best experts on this subject based on the ideXlab platform.
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efficacy of zero nitrous oxide emitting aerobic denitrifying bacterium methylobacterium gregans dc 1 in nitRate removal with strong auto Aggregation property
Bioresource Technology, 2019Co-Authors: Pei Hong, Yilin Shu, Chunbo Wang, Cuicui Tian, Oscar Omondi Donde, Bangding XiaoAbstract:Abstract A novel aerobic denitrifying strain Methylobacterium gregans DC-1 was isolated and identified. Strain DC-1 removed 98.4% of nitRate-nitrogen (NO3−-N) and 80.7% of total organic carbon with initial concentrations of 50 and 2400 mg/l, respectively. The N balance showed that most NO3−-N was converted to N2 (62.18%) without nitrous oxide (N2O) emission. Response surface analysis showed that the optimal conditions for total N removal were carbon (C):N ratio of 18.7, temperature of 26.8 °C, pH of 6.5 and shaking speed of 180 rpm. In combination with the N balance and successful amplification of napA, nirK and nosZ genes, the metabolic pathway was as follows: NO3−NO2− → NO → N2O → N2. Strain DC-1 had strong auto-Aggregation Rate (maximum 38.7%), produced large amounts of extracellular polymeric substances (EPS; maximum of 781.4 mg/g cell dry weight) and had corresponding strong hydrophobicity (maximum 83.2%). Pearson correlation analysis showed that EPS content and hydrophobicity were significantly positively correlated with auto-Aggregation.
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efficacy of zero nitrous oxide emitting aerobic denitrifying bacterium methylobacterium gregans dc 1 in nitRate removal with strong auto Aggregation property
Bioresource Technology, 2019Co-Authors: Pei Hong, Yilin Shu, Chunbo Wang, Cuicui Tian, Oscar Omondi Donde, Bangding XiaoAbstract:A novel aerobic denitrifying strain Methylobacterium gregans DC-1 was isolated and identified. Strain DC-1 removed 98.4% of nitRate-nitrogen (NO3--N) and 80.7% of total organic carbon with initial concentrations of 50 and 2400 mg/1, respectively. The N balance showed that most NO3--N was converted to N-2(62.18%) without nitrous oxide (N2O) emission. Response surface analysis showed that the optimal conditions for total N removal were carbon (C):N ratio of 18.7, temperature of 26.8 degrees C, pH of 6.5 and shaking speed of 180 rpm. In combination with the N balance and successful amplification of napA, nirK and nosZ genes, the metabolic pathway was as follows: NO3-NO2- -> NO -> N-2(O) -> N-2. Strain DC-1 had strong auto-Aggregation Rate (maximum 38.7%), produced large amounts of extracellular polymeric substances (EPS; maximum of 781.4 mg/g cell dry weight) and had corresponding strong hydrophobicity (maximum 83.2%). Pearson correlation analysis showed that EPS content and hydrophobicity were significantly positively correlated with auto-Aggregation.
Jonathan N Webb - One of the best experts on this subject based on the ideXlab platform.
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partial molar volume surface area and hydration changes for equilibrium unfolding and formation of Aggregation transition state high pressure and cosolute studies on recombinant human ifn γ
Proceedings of the National Academy of Sciences of the United States of America, 2001Co-Authors: Jonathan N Webb, Serena D Webb, Jeffrey L Cleland, John F Carpenter, Theodore W RandolphAbstract:The equilibrium dissociation of recombinant human IFN-γ was monitored as a function of pressure and sucrose concentration. The partial molar volume change for dissociation was −209 ± 13 ml/mol of dimer. The specific molar surface area change for dissociation was 12.7 ± 1.6 nm2/molecule of dimer. The first-order Aggregation Rate of recombinant human IFN-γ in 0.45 M guanidine hydrochloride was studied as a function of sucrose concentration and pressure. Aggregation proceeded through a transition-state species, N*. Sucrose reduced Aggregation Rate by shifting the equilibrium between native state (N) and N* toward the more compact N. Pressure increased Aggregation Rate through increased solvation of the protein, which exposes more surface area, thus shifting the equilibrium away from N toward N*. The changes in partial molar volume and specific molar surface area between the N* and N were −41 ± 9 ml/mol of dimer and 3.5 ± 0.2 nm2/molecule, respectively. Thus, the structural change required for the formation of the transition state for Aggregation is small relative to the difference between N and the dissociated state. Changes in waters of hydration were estimated from both specific molar surface area and partial molar volume data. From partial molar volume data, estimates were 25 and 128 mol H2O/mol dimer for formation of the Aggregation transition state and for dissociation, respectively. From surface area data, estimates were 27 and 98 mol H2O/mol dimer. Osmotic stress theory yielded values ≈4-fold larger for both transitions.
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partial molar volume surface area and hydration changes for equilibrium unfolding and formation of Aggregation transition state high pressure and cosolute studies on recombinant human ifn γ
Proceedings of the National Academy of Sciences of the United States of America, 2001Co-Authors: Jonathan N Webb, Serena D Webb, Jeffrey L Cleland, John F Carpenter, Theodore W RandolphAbstract:The equilibrium dissociation of recombinant human IFN-gamma was monitored as a function of pressure and sucrose concentration. The partial molar volume change for dissociation was -209 +/- 13 ml/mol of dimer. The specific molar surface area change for dissociation was 12.7 +/- 1.6 nm2/molecule of dimer. The first-order Aggregation Rate of recombinant human IFN-gamma in 0.45 M guanidine hydrochloride was studied as a function of sucrose concentration and pressure. Aggregation proceeded through a transition-state species, N*. Sucrose reduced Aggregation Rate by shifting the equilibrium between native state (N) and N* toward the more compact N. Pressure increased Aggregation Rate through increased solvation of the protein, which exposes more surface area, thus shifting the equilibrium away from N toward N*. The changes in partial molar volume and specific molar surface area between the N* and N were -41 +/- 9 ml/mol of dimer and 3.5 +/- 0.2 nm2/molecule, respectively. Thus, the structural change required for the formation of the transition state for Aggregation is small relative to the difference between N and the dissociated state. Changes in waters of hydration were estimated from both specific molar surface area and partial molar volume data. From partial molar volume data, estimates were 25 and 128 mol H2O/mol dimer for formation of the Aggregation transition state and for dissociation, respectively. From surface area data, estimates were 27 and 98 mol H2O/mol dimer. Osmotic stress theory yielded values approximately 4-fold larger for both transitions.
Oscar Omondi Donde - One of the best experts on this subject based on the ideXlab platform.
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efficacy of zero nitrous oxide emitting aerobic denitrifying bacterium methylobacterium gregans dc 1 in nitRate removal with strong auto Aggregation property
Bioresource Technology, 2019Co-Authors: Pei Hong, Yilin Shu, Chunbo Wang, Cuicui Tian, Oscar Omondi Donde, Bangding XiaoAbstract:Abstract A novel aerobic denitrifying strain Methylobacterium gregans DC-1 was isolated and identified. Strain DC-1 removed 98.4% of nitRate-nitrogen (NO3−-N) and 80.7% of total organic carbon with initial concentrations of 50 and 2400 mg/l, respectively. The N balance showed that most NO3−-N was converted to N2 (62.18%) without nitrous oxide (N2O) emission. Response surface analysis showed that the optimal conditions for total N removal were carbon (C):N ratio of 18.7, temperature of 26.8 °C, pH of 6.5 and shaking speed of 180 rpm. In combination with the N balance and successful amplification of napA, nirK and nosZ genes, the metabolic pathway was as follows: NO3−NO2− → NO → N2O → N2. Strain DC-1 had strong auto-Aggregation Rate (maximum 38.7%), produced large amounts of extracellular polymeric substances (EPS; maximum of 781.4 mg/g cell dry weight) and had corresponding strong hydrophobicity (maximum 83.2%). Pearson correlation analysis showed that EPS content and hydrophobicity were significantly positively correlated with auto-Aggregation.
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efficacy of zero nitrous oxide emitting aerobic denitrifying bacterium methylobacterium gregans dc 1 in nitRate removal with strong auto Aggregation property
Bioresource Technology, 2019Co-Authors: Pei Hong, Yilin Shu, Chunbo Wang, Cuicui Tian, Oscar Omondi Donde, Bangding XiaoAbstract:A novel aerobic denitrifying strain Methylobacterium gregans DC-1 was isolated and identified. Strain DC-1 removed 98.4% of nitRate-nitrogen (NO3--N) and 80.7% of total organic carbon with initial concentrations of 50 and 2400 mg/1, respectively. The N balance showed that most NO3--N was converted to N-2(62.18%) without nitrous oxide (N2O) emission. Response surface analysis showed that the optimal conditions for total N removal were carbon (C):N ratio of 18.7, temperature of 26.8 degrees C, pH of 6.5 and shaking speed of 180 rpm. In combination with the N balance and successful amplification of napA, nirK and nosZ genes, the metabolic pathway was as follows: NO3-NO2- -> NO -> N-2(O) -> N-2. Strain DC-1 had strong auto-Aggregation Rate (maximum 38.7%), produced large amounts of extracellular polymeric substances (EPS; maximum of 781.4 mg/g cell dry weight) and had corresponding strong hydrophobicity (maximum 83.2%). Pearson correlation analysis showed that EPS content and hydrophobicity were significantly positively correlated with auto-Aggregation.