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Mogens Norn – One of the best experts on this subject based on the ideXlab platform.

  • SIDE‐EFFECTS OF VITAL STAINING WITH TETRAZOLIUM‐Alcian Blue
    Acta Ophthalmologica, 2009
    Co-Authors: Mogens Norn

    Abstract:

    Vital staining of cornea and conjunctiva with a mixture of tetrazolium and Alcian Blue effected a permanent green colouring of connective tissue or precipitation of red crystals in 0.6 %> of 1,828 eyes. This colouring subsided in the course of weeks or months.

    Tattooing can be avoided by omitting vital staining with tetrazolium-Alcian Blue in eyes with connective tissue left uncovered by epithelium (from corrosion or deep keratitis, or resulting immediately after removal of sutures or during operation).

  • side effects of vital staining with tetrazolium Alcian Blue
    Acta Ophthalmologica, 2009
    Co-Authors: Mogens Norn

    Abstract:

    Vital staining of cornea and conjunctiva with a mixture of tetrazolium and Alcian Blue effected a permanent green colouring of connective tissue or precipitation of red crystals in 0.6 %> of 1,828 eyes. This colouring subsided in the course of weeks or months.

    Tattooing can be avoided by omitting vital staining with tetrazolium-Alcian Blue in eyes with connective tissue left uncovered by epithelium (from corrosion or deep keratitis, or resulting immediately after removal of sutures or during operation).

David Y Graham – One of the best experts on this subject based on the ideXlab platform.

  • a new triple stain for helicobacter pylori suitable for the autostainer carbol fuchsin Alcian Blue hematoxylin eosin
    Archives of Pathology & Laboratory Medicine, 1998
    Co-Authors: Hala M T Elzimaity, H Ota, S Scott, D E Killen, David Y Graham

    Abstract:

    OBJECTIVE To develop an inexpensive stain to simultaneously visualize gastric morphology and Helicobacter pylori. METHODS Gastric biopsies were stained with Genta stain using manual methods, and with carbol fuchsin/Alcian Blue/hematoxylin-eosin using an automatic slide stainer (Sakura DRS-601). Slides were then coded and interpreted by 2 pathologists. Helicobacter pylori was scored using a visual scale (0 [none] to 5 [maximum]). RESULTS One hundred slides were scored; H pylori was present in 64%. Carbol fuchsin/Alcian Blue/hematoxylin-eosin stain gave excellent demonstration of gastric morphology. All positive cases (score > or =2) were correctly interpreted. Thirty-six slides had a score of 1 (< or =2 bacteria per entire slide). Of these, 10 were scored negative by Genta stain and 12 were scored negative by the carbol fuchsin/Alcian Blue/hematoxylin-eosin stain (P = not significant). Hematoxylin-eosin was significantly less accurate than either of the other 2 stains (P < .02). CONCLUSION The carbol fuchsin/Alcian Blue/hematoxylin-eosin (El-Zimaity) stain is an economical stain suitable for simultaneous visualization of H pylori infection and gastric morphology.

  • A new triple stain for Helicobacter pylori suitable for the autostainer: carbol fuchsin/Alcian Blue/hematoxylin-eosin.
    Archives of pathology & laboratory medicine, 1998
    Co-Authors: Hala M.t. El-zimaity, S Scott, H Ota, D E Killen, David Y Graham

    Abstract:

    OBJECTIVE To develop an inexpensive stain to simultaneously visualize gastric morphology and Helicobacter pylori. METHODS Gastric biopsies were stained with Genta stain using manual methods, and with carbol fuchsin/Alcian Blue/hematoxylin-eosin using an automatic slide stainer (Sakura DRS-601). Slides were then coded and interpreted by 2 pathologists. Helicobacter pylori was scored using a visual scale (0 [none] to 5 [maximum]). RESULTS One hundred slides were scored; H pylori was present in 64%. Carbol fuchsin/Alcian Blue/hematoxylin-eosin stain gave excellent demonstration of gastric morphology. All positive cases (score > or =2) were correctly interpreted. Thirty-six slides had a score of 1 (< or =2 bacteria per entire slide). Of these, 10 were scored negative by Genta stain and 12 were scored negative by the carbol fuchsin/Alcian Blue/hematoxylin-eosin stain (P = not significant). Hematoxylin-eosin was significantly less accurate than either of the other 2 stains (P < .02). CONCLUSION The carbol fuchsin/Alcian Blue/hematoxylin-eosin (El-Zimaity) stain is an economical stain suitable for simultaneous visualization of H pylori infection and gastric morphology.

H Ota – One of the best experts on this subject based on the ideXlab platform.

  • a new triple stain for helicobacter pylori suitable for the autostainer carbol fuchsin Alcian Blue hematoxylin eosin
    Archives of Pathology & Laboratory Medicine, 1998
    Co-Authors: Hala M T Elzimaity, H Ota, S Scott, D E Killen, David Y Graham

    Abstract:

    OBJECTIVE To develop an inexpensive stain to simultaneously visualize gastric morphology and Helicobacter pylori. METHODS Gastric biopsies were stained with Genta stain using manual methods, and with carbol fuchsin/Alcian Blue/hematoxylin-eosin using an automatic slide stainer (Sakura DRS-601). Slides were then coded and interpreted by 2 pathologists. Helicobacter pylori was scored using a visual scale (0 [none] to 5 [maximum]). RESULTS One hundred slides were scored; H pylori was present in 64%. Carbol fuchsin/Alcian Blue/hematoxylin-eosin stain gave excellent demonstration of gastric morphology. All positive cases (score > or =2) were correctly interpreted. Thirty-six slides had a score of 1 (< or =2 bacteria per entire slide). Of these, 10 were scored negative by Genta stain and 12 were scored negative by the carbol fuchsin/Alcian Blue/hematoxylin-eosin stain (P = not significant). Hematoxylin-eosin was significantly less accurate than either of the other 2 stains (P < .02). CONCLUSION The carbol fuchsin/Alcian Blue/hematoxylin-eosin (El-Zimaity) stain is an economical stain suitable for simultaneous visualization of H pylori infection and gastric morphology.

  • A new triple stain for Helicobacter pylori suitable for the autostainer: carbol fuchsin/Alcian Blue/hematoxylin-eosin.
    Archives of pathology & laboratory medicine, 1998
    Co-Authors: Hala M.t. El-zimaity, S Scott, H Ota, D E Killen, David Y Graham

    Abstract:

    OBJECTIVE To develop an inexpensive stain to simultaneously visualize gastric morphology and Helicobacter pylori. METHODS Gastric biopsies were stained with Genta stain using manual methods, and with carbol fuchsin/Alcian Blue/hematoxylin-eosin using an automatic slide stainer (Sakura DRS-601). Slides were then coded and interpreted by 2 pathologists. Helicobacter pylori was scored using a visual scale (0 [none] to 5 [maximum]). RESULTS One hundred slides were scored; H pylori was present in 64%. Carbol fuchsin/Alcian Blue/hematoxylin-eosin stain gave excellent demonstration of gastric morphology. All positive cases (score > or =2) were correctly interpreted. Thirty-six slides had a score of 1 (< or =2 bacteria per entire slide). Of these, 10 were scored negative by Genta stain and 12 were scored negative by the carbol fuchsin/Alcian Blue/hematoxylin-eosin stain (P = not significant). Hematoxylin-eosin was significantly less accurate than either of the other 2 stains (P < .02). CONCLUSION The carbol fuchsin/Alcian Blue/hematoxylin-eosin (El-Zimaity) stain is an economical stain suitable for simultaneous visualization of H pylori infection and gastric morphology.