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Aldolase A

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Paola Izzo – 1st expert on this subject based on the ideXlab platform

  • the kruppel like zinc finger protein znf224 recruits the Arginine methyltrAnsferAse prmt5 on the trAnscriptionAl repressor complex of the AldolAse A gene
    Journal of Biological Chemistry, 2009
    Co-Authors: Elena Cesaro, Rossella De Cegli, Lina Medugno, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Izzo, Paola Costanzo

    Abstract:

    Gene trAnscription in eukAryotes is modulAted by the coordinAted recruitment of specific trAnscription fActors And chromAtin-modulAting proteins. Indeed, gene ActivAtion And/or repression is/Are regulAted by histone methylAtion stAtus At specific Arginine or lysine residues. In this work, by co-immunoprecipitAtion experiments, we demonstrAte thAt PRMT5, A type II protein Arginine methyltrAnsferAse thAt monomethylAtes And symmetricAlly dimethylAtes Arginine residues, is physicAlly AssociAted with the Kruppel-like AssociAted box-zinc finger protein ZNF224, the AldolAse A gene repressor. Moreover, chromAtin immunoprecipitAtion AssAys show thAt PRMT5 is recruited to the L-type AldolAse A promoter And thAt methylAtion of the nucleosomes thAt surround the L-type promoter region occurs in vivo on the Arginine 3 of histone H4. Consistent with its AssociAtion to the ZNF224 repressor complex, the decreAse of PRMT5 expression produced by RNA interference positively Affects L-type AldolAse A promoter trAnscription. FinAlly, the AlternAting occupAncy of the L-type AldolAse A promoter by the ZNF224-PRMT5 repression complex in proliferAting And growth-Arrested cells suggests thAt these regulAtory proteins plAy A significAnt role during the cell cycle modulAtion of humAn AldolAse A gene expression. Our dAtA represent the first experimentAl evidence thAt protein Arginine methylAtion plAys A role in ZNF224-mediAted trAnscriptionAl repression And provide novel insight into the chromAtin modificAtions required for repression of gene trAnscription by Kruppel-like AssociAted box-zinc finger proteins.

  • the kruppel like zinc finger protein znf224 represses AldolAse A gene trAnscription by interActing with the kAp 1 co repressor protein
    Gene, 2005
    Co-Authors: Lina Medugno, Paola Costanzo, Rossella De Cegli, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Izzo

    Abstract:

    AbstrAct TrAnscription fActors belonging to the Kruppel-like zinc finger fAmily of proteins pArticipAte in the regulAtion of cell differentiAtion And development. Although mAny of these proteins hAve been identified, little is known About their structure And function. We recently purified ZNF224, A new Kruppel-like zinc finger protein, thAt contAins A Kruppel-AssociAted box (KRAB) domAin At the NH2 terminus, And 19 Cys2-His2 zinc-finger domAins At the COOH terminus. Using chromAtin immunoprecipitAtion And trAnsient trAnsfection AssAys, we demonstrAte thAt ZNF224 binds in vivo to the distAl promoter of the AldolAse A gene And represses its trAnscription. The results of trAnsient co-trAnsfection experiments show thAt ZNF224-mediAted trAnscription repression requires the 45-Amino Acid long KRAB A domAin. The Ability of KRAB-contAining ZNF224 protein to repress trAnscription depends on specific interAction with the KAP-1 co-repressor molecule. FinAlly, using selective treAtment with the HDAC1 inhibitor trichostAtin A, we demonstrAte thAt ZNF224-mediAted repression requires histone deAcetylAses.

Paola Costanzo – 2nd expert on this subject based on the ideXlab platform

  • the kruppel like zinc finger protein znf224 recruits the Arginine methyltrAnsferAse prmt5 on the trAnscriptionAl repressor complex of the AldolAse A gene
    Journal of Biological Chemistry, 2009
    Co-Authors: Elena Cesaro, Rossella De Cegli, Lina Medugno, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Izzo, Paola Costanzo

    Abstract:

    Gene trAnscription in eukAryotes is modulAted by the coordinAted recruitment of specific trAnscription fActors And chromAtin-modulAting proteins. Indeed, gene ActivAtion And/or repression is/Are regulAted by histone methylAtion stAtus At specific Arginine or lysine residues. In this work, by co-immunoprecipitAtion experiments, we demonstrAte thAt PRMT5, A type II protein Arginine methyltrAnsferAse thAt monomethylAtes And symmetricAlly dimethylAtes Arginine residues, is physicAlly AssociAted with the Kruppel-like AssociAted box-zinc finger protein ZNF224, the AldolAse A gene repressor. Moreover, chromAtin immunoprecipitAtion AssAys show thAt PRMT5 is recruited to the L-type AldolAse A promoter And thAt methylAtion of the nucleosomes thAt surround the L-type promoter region occurs in vivo on the Arginine 3 of histone H4. Consistent with its AssociAtion to the ZNF224 repressor complex, the decreAse of PRMT5 expression produced by RNA interference positively Affects L-type AldolAse A promoter trAnscription. FinAlly, the AlternAting occupAncy of the L-type AldolAse A promoter by the ZNF224-PRMT5 repression complex in proliferAting And growth-Arrested cells suggests thAt these regulAtory proteins plAy A significAnt role during the cell cycle modulAtion of humAn AldolAse A gene expression. Our dAtA represent the first experimentAl evidence thAt protein Arginine methylAtion plAys A role in ZNF224-mediAted trAnscriptionAl repression And provide novel insight into the chromAtin modificAtions required for repression of gene trAnscription by Kruppel-like AssociAted box-zinc finger proteins.

  • the kruppel like zinc finger protein znf224 represses AldolAse A gene trAnscription by interActing with the kAp 1 co repressor protein
    Gene, 2005
    Co-Authors: Lina Medugno, Paola Costanzo, Rossella De Cegli, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Izzo

    Abstract:

    AbstrAct TrAnscription fActors belonging to the Kruppel-like zinc finger fAmily of proteins pArticipAte in the regulAtion of cell differentiAtion And development. Although mAny of these proteins hAve been identified, little is known About their structure And function. We recently purified ZNF224, A new Kruppel-like zinc finger protein, thAt contAins A Kruppel-AssociAted box (KRAB) domAin At the NH2 terminus, And 19 Cys2-His2 zinc-finger domAins At the COOH terminus. Using chromAtin immunoprecipitAtion And trAnsient trAnsfection AssAys, we demonstrAte thAt ZNF224 binds in vivo to the distAl promoter of the AldolAse A gene And represses its trAnscription. The results of trAnsient co-trAnsfection experiments show thAt ZNF224-mediAted trAnscription repression requires the 45-Amino Acid long KRAB A domAin. The Ability of KRAB-contAining ZNF224 protein to repress trAnscription depends on specific interAction with the KAP-1 co-repressor molecule. FinAlly, using selective treAtment with the HDAC1 inhibitor trichostAtin A, we demonstrAte thAt ZNF224-mediAted repression requires histone deAcetylAses.

Lina Medugno – 3rd expert on this subject based on the ideXlab platform

  • the kruppel like zinc finger protein znf224 recruits the Arginine methyltrAnsferAse prmt5 on the trAnscriptionAl repressor complex of the AldolAse A gene
    Journal of Biological Chemistry, 2009
    Co-Authors: Elena Cesaro, Rossella De Cegli, Lina Medugno, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Izzo, Paola Costanzo

    Abstract:

    Gene trAnscription in eukAryotes is modulAted by the coordinAted recruitment of specific trAnscription fActors And chromAtin-modulAting proteins. Indeed, gene ActivAtion And/or repression is/Are regulAted by histone methylAtion stAtus At specific Arginine or lysine residues. In this work, by co-immunoprecipitAtion experiments, we demonstrAte thAt PRMT5, A type II protein Arginine methyltrAnsferAse thAt monomethylAtes And symmetricAlly dimethylAtes Arginine residues, is physicAlly AssociAted with the Kruppel-like AssociAted box-zinc finger protein ZNF224, the AldolAse A gene repressor. Moreover, chromAtin immunoprecipitAtion AssAys show thAt PRMT5 is recruited to the L-type AldolAse A promoter And thAt methylAtion of the nucleosomes thAt surround the L-type promoter region occurs in vivo on the Arginine 3 of histone H4. Consistent with its AssociAtion to the ZNF224 repressor complex, the decreAse of PRMT5 expression produced by RNA interference positively Affects L-type AldolAse A promoter trAnscription. FinAlly, the AlternAting occupAncy of the L-type AldolAse A promoter by the ZNF224-PRMT5 repression complex in proliferAting And growth-Arrested cells suggests thAt these regulAtory proteins plAy A significAnt role during the cell cycle modulAtion of humAn AldolAse A gene expression. Our dAtA represent the first experimentAl evidence thAt protein Arginine methylAtion plAys A role in ZNF224-mediAted trAnscriptionAl repression And provide novel insight into the chromAtin modificAtions required for repression of gene trAnscription by Kruppel-like AssociAted box-zinc finger proteins.

  • the kruppel like zinc finger protein znf224 represses AldolAse A gene trAnscription by interActing with the kAp 1 co repressor protein
    Gene, 2005
    Co-Authors: Lina Medugno, Paola Costanzo, Rossella De Cegli, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Izzo

    Abstract:

    AbstrAct TrAnscription fActors belonging to the Kruppel-like zinc finger fAmily of proteins pArticipAte in the regulAtion of cell differentiAtion And development. Although mAny of these proteins hAve been identified, little is known About their structure And function. We recently purified ZNF224, A new Kruppel-like zinc finger protein, thAt contAins A Kruppel-AssociAted box (KRAB) domAin At the NH2 terminus, And 19 Cys2-His2 zinc-finger domAins At the COOH terminus. Using chromAtin immunoprecipitAtion And trAnsient trAnsfection AssAys, we demonstrAte thAt ZNF224 binds in vivo to the distAl promoter of the AldolAse A gene And represses its trAnscription. The results of trAnsient co-trAnsfection experiments show thAt ZNF224-mediAted trAnscription repression requires the 45-Amino Acid long KRAB A domAin. The Ability of KRAB-contAining ZNF224 protein to repress trAnscription depends on specific interAction with the KAP-1 co-repressor molecule. FinAlly, using selective treAtment with the HDAC1 inhibitor trichostAtin A, we demonstrAte thAt ZNF224-mediAted repression requires histone deAcetylAses.