The Experts below are selected from a list of 189 Experts worldwide ranked by ideXlab platform
Paola Izzo - One of the best experts on this subject based on the ideXlab platform.
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the kruppel like zinc finger protein znf224 recruits the Arginine methyltrAnsferAse prmt5 on the trAnscriptionAl repressor complex of the AldolAse A gene
Journal of Biological Chemistry, 2009Co-Authors: Elena Cesaro, Rossella De Cegli, Lina Medugno, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Izzo, Paola CostanzoAbstract:Gene trAnscription in eukAryotes is modulAted by the coordinAted recruitment of specific trAnscription fActors And chromAtin-modulAting proteins. Indeed, gene ActivAtion And/or repression is/Are regulAted by histone methylAtion stAtus At specific Arginine or lysine residues. In this work, by co-immunoprecipitAtion experiments, we demonstrAte thAt PRMT5, A type II protein Arginine methyltrAnsferAse thAt monomethylAtes And symmetricAlly dimethylAtes Arginine residues, is physicAlly AssociAted with the Kruppel-like AssociAted box-zinc finger protein ZNF224, the AldolAse A gene repressor. Moreover, chromAtin immunoprecipitAtion AssAys show thAt PRMT5 is recruited to the L-type AldolAse A promoter And thAt methylAtion of the nucleosomes thAt surround the L-type promoter region occurs in vivo on the Arginine 3 of histone H4. Consistent with its AssociAtion to the ZNF224 repressor complex, the decreAse of PRMT5 expression produced by RNA interference positively Affects L-type AldolAse A promoter trAnscription. FinAlly, the AlternAting occupAncy of the L-type AldolAse A promoter by the ZNF224-PRMT5 repression complex in proliferAting And growth-Arrested cells suggests thAt these regulAtory proteins plAy A significAnt role during the cell cycle modulAtion of humAn AldolAse A gene expression. Our dAtA represent the first experimentAl evidence thAt protein Arginine methylAtion plAys A role in ZNF224-mediAted trAnscriptionAl repression And provide novel insight into the chromAtin modificAtions required for repression of gene trAnscription by Kruppel-like AssociAted box-zinc finger proteins.
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the kruppel like zinc finger protein znf224 represses AldolAse A gene trAnscription by interActing with the kAp 1 co repressor protein
Gene, 2005Co-Authors: Lina Medugno, Rossella De Cegli, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Costanzo, Paola IzzoAbstract:AbstrAct TrAnscription fActors belonging to the Kruppel-like zinc finger fAmily of proteins pArticipAte in the regulAtion of cell differentiAtion And development. Although mAny of these proteins hAve been identified, little is known About their structure And function. We recently purified ZNF224, A new Kruppel-like zinc finger protein, thAt contAins A Kruppel-AssociAted box (KRAB) domAin At the NH2 terminus, And 19 Cys2-His2 zinc-finger domAins At the COOH terminus. Using chromAtin immunoprecipitAtion And trAnsient trAnsfection AssAys, we demonstrAte thAt ZNF224 binds in vivo to the distAl promoter of the AldolAse A gene And represses its trAnscription. The results of trAnsient co-trAnsfection experiments show thAt ZNF224-mediAted trAnscription repression requires the 45-Amino Acid long KRAB A domAin. The Ability of KRAB-contAining ZNF224 protein to repress trAnscription depends on specific interAction with the KAP-1 co-repressor molecule. FinAlly, using selective treAtment with the HDAC1 inhibitor trichostAtin A, we demonstrAte thAt ZNF224-mediAted repression requires histone deAcetylAses.
Paola Costanzo - One of the best experts on this subject based on the ideXlab platform.
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the kruppel like zinc finger protein znf224 recruits the Arginine methyltrAnsferAse prmt5 on the trAnscriptionAl repressor complex of the AldolAse A gene
Journal of Biological Chemistry, 2009Co-Authors: Elena Cesaro, Rossella De Cegli, Lina Medugno, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Izzo, Paola CostanzoAbstract:Gene trAnscription in eukAryotes is modulAted by the coordinAted recruitment of specific trAnscription fActors And chromAtin-modulAting proteins. Indeed, gene ActivAtion And/or repression is/Are regulAted by histone methylAtion stAtus At specific Arginine or lysine residues. In this work, by co-immunoprecipitAtion experiments, we demonstrAte thAt PRMT5, A type II protein Arginine methyltrAnsferAse thAt monomethylAtes And symmetricAlly dimethylAtes Arginine residues, is physicAlly AssociAted with the Kruppel-like AssociAted box-zinc finger protein ZNF224, the AldolAse A gene repressor. Moreover, chromAtin immunoprecipitAtion AssAys show thAt PRMT5 is recruited to the L-type AldolAse A promoter And thAt methylAtion of the nucleosomes thAt surround the L-type promoter region occurs in vivo on the Arginine 3 of histone H4. Consistent with its AssociAtion to the ZNF224 repressor complex, the decreAse of PRMT5 expression produced by RNA interference positively Affects L-type AldolAse A promoter trAnscription. FinAlly, the AlternAting occupAncy of the L-type AldolAse A promoter by the ZNF224-PRMT5 repression complex in proliferAting And growth-Arrested cells suggests thAt these regulAtory proteins plAy A significAnt role during the cell cycle modulAtion of humAn AldolAse A gene expression. Our dAtA represent the first experimentAl evidence thAt protein Arginine methylAtion plAys A role in ZNF224-mediAted trAnscriptionAl repression And provide novel insight into the chromAtin modificAtions required for repression of gene trAnscription by Kruppel-like AssociAted box-zinc finger proteins.
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the kruppel like zinc finger protein znf224 represses AldolAse A gene trAnscription by interActing with the kAp 1 co repressor protein
Gene, 2005Co-Authors: Lina Medugno, Rossella De Cegli, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Costanzo, Paola IzzoAbstract:AbstrAct TrAnscription fActors belonging to the Kruppel-like zinc finger fAmily of proteins pArticipAte in the regulAtion of cell differentiAtion And development. Although mAny of these proteins hAve been identified, little is known About their structure And function. We recently purified ZNF224, A new Kruppel-like zinc finger protein, thAt contAins A Kruppel-AssociAted box (KRAB) domAin At the NH2 terminus, And 19 Cys2-His2 zinc-finger domAins At the COOH terminus. Using chromAtin immunoprecipitAtion And trAnsient trAnsfection AssAys, we demonstrAte thAt ZNF224 binds in vivo to the distAl promoter of the AldolAse A gene And represses its trAnscription. The results of trAnsient co-trAnsfection experiments show thAt ZNF224-mediAted trAnscription repression requires the 45-Amino Acid long KRAB A domAin. The Ability of KRAB-contAining ZNF224 protein to repress trAnscription depends on specific interAction with the KAP-1 co-repressor molecule. FinAlly, using selective treAtment with the HDAC1 inhibitor trichostAtin A, we demonstrAte thAt ZNF224-mediAted repression requires histone deAcetylAses.
Lina Medugno - One of the best experts on this subject based on the ideXlab platform.
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the kruppel like zinc finger protein znf224 recruits the Arginine methyltrAnsferAse prmt5 on the trAnscriptionAl repressor complex of the AldolAse A gene
Journal of Biological Chemistry, 2009Co-Authors: Elena Cesaro, Rossella De Cegli, Lina Medugno, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Izzo, Paola CostanzoAbstract:Gene trAnscription in eukAryotes is modulAted by the coordinAted recruitment of specific trAnscription fActors And chromAtin-modulAting proteins. Indeed, gene ActivAtion And/or repression is/Are regulAted by histone methylAtion stAtus At specific Arginine or lysine residues. In this work, by co-immunoprecipitAtion experiments, we demonstrAte thAt PRMT5, A type II protein Arginine methyltrAnsferAse thAt monomethylAtes And symmetricAlly dimethylAtes Arginine residues, is physicAlly AssociAted with the Kruppel-like AssociAted box-zinc finger protein ZNF224, the AldolAse A gene repressor. Moreover, chromAtin immunoprecipitAtion AssAys show thAt PRMT5 is recruited to the L-type AldolAse A promoter And thAt methylAtion of the nucleosomes thAt surround the L-type promoter region occurs in vivo on the Arginine 3 of histone H4. Consistent with its AssociAtion to the ZNF224 repressor complex, the decreAse of PRMT5 expression produced by RNA interference positively Affects L-type AldolAse A promoter trAnscription. FinAlly, the AlternAting occupAncy of the L-type AldolAse A promoter by the ZNF224-PRMT5 repression complex in proliferAting And growth-Arrested cells suggests thAt these regulAtory proteins plAy A significAnt role during the cell cycle modulAtion of humAn AldolAse A gene expression. Our dAtA represent the first experimentAl evidence thAt protein Arginine methylAtion plAys A role in ZNF224-mediAted trAnscriptionAl repression And provide novel insight into the chromAtin modificAtions required for repression of gene trAnscription by Kruppel-like AssociAted box-zinc finger proteins.
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the kruppel like zinc finger protein znf224 represses AldolAse A gene trAnscription by interActing with the kAp 1 co repressor protein
Gene, 2005Co-Authors: Lina Medugno, Rossella De Cegli, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Costanzo, Paola IzzoAbstract:AbstrAct TrAnscription fActors belonging to the Kruppel-like zinc finger fAmily of proteins pArticipAte in the regulAtion of cell differentiAtion And development. Although mAny of these proteins hAve been identified, little is known About their structure And function. We recently purified ZNF224, A new Kruppel-like zinc finger protein, thAt contAins A Kruppel-AssociAted box (KRAB) domAin At the NH2 terminus, And 19 Cys2-His2 zinc-finger domAins At the COOH terminus. Using chromAtin immunoprecipitAtion And trAnsient trAnsfection AssAys, we demonstrAte thAt ZNF224 binds in vivo to the distAl promoter of the AldolAse A gene And represses its trAnscription. The results of trAnsient co-trAnsfection experiments show thAt ZNF224-mediAted trAnscription repression requires the 45-Amino Acid long KRAB A domAin. The Ability of KRAB-contAining ZNF224 protein to repress trAnscription depends on specific interAction with the KAP-1 co-repressor molecule. FinAlly, using selective treAtment with the HDAC1 inhibitor trichostAtin A, we demonstrAte thAt ZNF224-mediAted repression requires histone deAcetylAses.
Basil J Petrof - One of the best experts on this subject based on the ideXlab platform.
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Six1 And EyA1 expression cAn reprogrAm Adult muscle from the slow-twitch phenotype into the fAst-twitch phenotype.
Molecular and cellular biology, 2004Co-Authors: R. Grifone, Soledad Lopez, Kiyoshi Kawakami, Pin-xian Xu, Christine Laclef, Robert Kelly, Francis Spitz, Josiane Demignon, Jacques-emmanuel Guidotti, Basil J PetrofAbstract:Muscle fibers show greAt differences in their contrActile And metAbolic properties. This diversity enAbles skeletAl muscles to fulfill And AdApt to different tAsks. In this report, we show thAt the Six/EyA pAthwAy is implicAted in the estAblishment And mAintenAnce of the fAst-twitch skeletAl muscle phenotype. We demonstrAte thAt the MEF3/Six DNA binding element present in the AldolAse A pM promoter mediAtes the high level of ActivAtion of this promoter in fAst-twitch glycolytic (but not in slow-twitch) muscle fibers. We Also show thAt Among the Six And EyA gene products expressed in mouse skeletAl muscle, Six1 And EyA1 proteins AccumulAte preferentiAlly in the nuclei of fAst-twitch muscles. The forced expression of Six1 And EyA1 together in the slow-twitch soleus muscle induced A fiber-type trAnsition chArActerized by the replAcement of myosin heAvy chAin I And IIA isoforms by the fAster IIB And/or IIX isoforms, the ActivAtion of fAst-twitch fiber-specific genes, And A switch towArd glycolytic metAbolism. Collectively, these dAtA identify Six1 And EyA1 As the first trAnscriptionAl complex thAt is Able to reprogrAm Adult slow-twitch oxidAtive fibers towArd A fAst-twitch glycolytic phenotype.
Michela Grosso - One of the best experts on this subject based on the ideXlab platform.
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the kruppel like zinc finger protein znf224 recruits the Arginine methyltrAnsferAse prmt5 on the trAnscriptionAl repressor complex of the AldolAse A gene
Journal of Biological Chemistry, 2009Co-Authors: Elena Cesaro, Rossella De Cegli, Lina Medugno, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Izzo, Paola CostanzoAbstract:Gene trAnscription in eukAryotes is modulAted by the coordinAted recruitment of specific trAnscription fActors And chromAtin-modulAting proteins. Indeed, gene ActivAtion And/or repression is/Are regulAted by histone methylAtion stAtus At specific Arginine or lysine residues. In this work, by co-immunoprecipitAtion experiments, we demonstrAte thAt PRMT5, A type II protein Arginine methyltrAnsferAse thAt monomethylAtes And symmetricAlly dimethylAtes Arginine residues, is physicAlly AssociAted with the Kruppel-like AssociAted box-zinc finger protein ZNF224, the AldolAse A gene repressor. Moreover, chromAtin immunoprecipitAtion AssAys show thAt PRMT5 is recruited to the L-type AldolAse A promoter And thAt methylAtion of the nucleosomes thAt surround the L-type promoter region occurs in vivo on the Arginine 3 of histone H4. Consistent with its AssociAtion to the ZNF224 repressor complex, the decreAse of PRMT5 expression produced by RNA interference positively Affects L-type AldolAse A promoter trAnscription. FinAlly, the AlternAting occupAncy of the L-type AldolAse A promoter by the ZNF224-PRMT5 repression complex in proliferAting And growth-Arrested cells suggests thAt these regulAtory proteins plAy A significAnt role during the cell cycle modulAtion of humAn AldolAse A gene expression. Our dAtA represent the first experimentAl evidence thAt protein Arginine methylAtion plAys A role in ZNF224-mediAted trAnscriptionAl repression And provide novel insight into the chromAtin modificAtions required for repression of gene trAnscription by Kruppel-like AssociAted box-zinc finger proteins.
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the kruppel like zinc finger protein znf224 represses AldolAse A gene trAnscription by interActing with the kAp 1 co repressor protein
Gene, 2005Co-Authors: Lina Medugno, Rossella De Cegli, Francesca Florio, Michela Grosso, Angelo Lupo, Paola Costanzo, Paola IzzoAbstract:AbstrAct TrAnscription fActors belonging to the Kruppel-like zinc finger fAmily of proteins pArticipAte in the regulAtion of cell differentiAtion And development. Although mAny of these proteins hAve been identified, little is known About their structure And function. We recently purified ZNF224, A new Kruppel-like zinc finger protein, thAt contAins A Kruppel-AssociAted box (KRAB) domAin At the NH2 terminus, And 19 Cys2-His2 zinc-finger domAins At the COOH terminus. Using chromAtin immunoprecipitAtion And trAnsient trAnsfection AssAys, we demonstrAte thAt ZNF224 binds in vivo to the distAl promoter of the AldolAse A gene And represses its trAnscription. The results of trAnsient co-trAnsfection experiments show thAt ZNF224-mediAted trAnscription repression requires the 45-Amino Acid long KRAB A domAin. The Ability of KRAB-contAining ZNF224 protein to repress trAnscription depends on specific interAction with the KAP-1 co-repressor molecule. FinAlly, using selective treAtment with the HDAC1 inhibitor trichostAtin A, we demonstrAte thAt ZNF224-mediAted repression requires histone deAcetylAses.
