The Experts below are selected from a list of 159 Experts worldwide ranked by ideXlab platform

Jean-luc Couturier - One of the best experts on this subject based on the ideXlab platform.

Hideaki Yamada - One of the best experts on this subject based on the ideXlab platform.

  • primary structure of an Aliphatic Nitrile degrading enzyme Aliphatic nitrilase from rhodococcus rhodochrous k22 and expression of its gene and identification of its active site residue
    Biochemistry, 1992
    Co-Authors: Michihiko Kobayashi, Toru Nagasawa, Noriyuki Yanaka, Hideaki Yamada
    Abstract:

    Peptides obtained by cleavage of a Rhodococcus rhodochrous K22 nitrilase, which acts on Aliphatic Nitriles such as acryloNitrile, crotoNitrile, and glutaroNitrile, have been sequenced. The data allowed the design of oligonucleotide probes which were used to clone a nitrilase encoding gene. Plasmid pNK21, in which 2.05-kb sequence covering the region encoding the nitrilase was was placed under the control of the lac promoter, directed overproduction of enzymatically active nitrilase in response to addition of isopropyl beta-D-thiogalactopyranoside in Escherichia coli. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the cell extract showed that the amount of nitrilase was about 40% of the total soluble proteins, leading to the establishment of a simple purification of the nitrilase. The nucleotide sequence of the nitrilase gene predicts a protein composed of 383 amino acids (M(r) = 42,275), including only one cysteine. The amino acid sequence homology between the Rhodococcus nitrilase and the Klebsiella ozaenae bromoxynil nitrilase [Stalker et al. (1988) J. Biol. Chem. 263, 6310-6314] was 38.3%, and a unique cysteinyl residue (Cys-170) in the former nitrilase was conserved at the corresponding position in the latter nitrilase. Cys-170 of the Rhodococcus nitrilase was replaced by Ala or Ser by site-directed mutagenesis. Both mutations resulted in the complete loss of nitrilase activity, clearly indicating that this cysteinyl residue is essential for the catalytic activity.

  • Optimum culture conditions for the production of cobalt-containing Nitrile hydratase by Rhodococcus rhodochrous J1
    Applied Microbiology and Biotechnology, 1991
    Co-Authors: Toru Nagasawa, Koji Takeuchi, Vincenzo Nardi-dei, Yasuhiro Mihara, Hideaki Yamada
    Abstract:

    We sought the optimum conditions for production of Nitrile hydratase by Rhodococcus rhodochrous J1. The addiiion of both cobalt ions and an Aliphatic Nitrile or amide as an inducer was indispensable for the appearance of Nitrile hydratase activity in R. rhodochrous J1 cells. Crotonamide was an efficient inducer and, moreover, urea was found to be the most powerful inducer for the production of Nitrile hydratase. When R. rhodochrous J1 was cultivated under optimal conditions, the enzyme activity in the culture broth and the specific activity was approximately 32,000 and 512 times higher than the initially obtained levels, respectively. The Nitrile hydratase formed corresponded to more than 45% of the total soluble protein in urea-induced cells, as judged by quantitative evaluation of the gel track.

Xiaowei Miao - One of the best experts on this subject based on the ideXlab platform.

Johan Jacquemin - One of the best experts on this subject based on the ideXlab platform.

  • physical chemical characterization of binary mixtures of 1 butyl 1 methylpyrrolidinium bis trifluoromethyl sulfonyl imide and Aliphatic Nitrile solvents as potential electrolytes for electrochemical energy storage applications
    Journal of Chemical & Engineering Data, 2017
    Co-Authors: Alex R Neale, Christoph Schutter, Patrick Wilde, Peter Goodrich, Christopher Hardacre, Stefano Passerini, Andrea Balducci, Johan Jacquemin
    Abstract:

    In the scope of improving the energy and power densities of electrochemical double layer capacitors (EDLCs), the development of high performance electrolytes with enhanced operative voltages is imperative. The formulation of mixtures containing ionic liquids with organic molecular solvents is an important strategy in the pursuit of developing highly electrochemically stable and safe materials while retaining fast transport properties for high power applications. In this work, we report on the physical–chemical investigations into binary mixtures containing the ionic liquid 1-butyl-1-methylpyrrolidinium bis{(trifluoromethyl)sulfonyl}imide with one monoNitrile solvent, butyroNitrile, and two diNitrile solvents, glutaroNitrile and adipoNitrile, as potential electrolytes for EDLCs. The thermal, volumetric, and transport properties of the binary mixtures are investigated as functions of the electrolyte composition and temperature. Furthermore, the electrolyte composition which exhibits the highest conductivity...

  • the use of binary mixtures of 1 butyl 1 methylpyrrolidinium bis trifluoromethyl sulfonyl imide and Aliphatic Nitrile solvents as electrolyte for supercapacitors
    Electrochimica Acta, 2016
    Co-Authors: Christoph Schutter, Alex R Neale, Patrick Wilde, Peter Goodrich, Christopher Hardacre, Stefano Passerini, Johan Jacquemin, Andrea Balducci
    Abstract:

    Abstract The development of high voltage electrolytes is one of the key aspects for increasing both energy and power density of electrochemical double layer capacitors (EDLCs). The usage of blends of ionic liquids and organic solvents has been considered as a feasible strategy since these electrolytes combine high usable voltages and good transport properties at the same time. In this work, the ionic liquid 1-butyl-1-methylpyrrolidinium bis{(trifluoromethyl)sulfonyl}imide ([Pyrr14][TFSI]) was mixed with two Nitrile-based organic solvents, namely butyroNitrile and adipoNitrile, and the resulting blends were investigated regarding their usage in electrochemical double layer capacitors. Both blends have a high electrochemical stability, which was confirmed by prolonged float tests at 3.2 V, as well as, good transport properties. In fact, the butyroNitrile blend reaches a conductivity of 17.14 mS·cm−1 and a viscosity of 2.46 mPa·s at 20 °C, which is better than the state-of-the-art electrolyte (1 mol·dm−3 of tetraethylammonium tetrafluoroborate in propylene carbonate).

  • Physical–Chemical Characterization of Binary Mixtures of 1-Butyl-1-methylpyrrolidinium Bis{(trifluoromethyl)sulfonyl}imide and Aliphatic Nitrile Solvents as Potential Electrolytes for Electrochemical Energy Storage Applications
    Journal of Chemical and Engineering Data, 2016
    Co-Authors: Alex Neale, Christoph Schutter, Patrick Wilde, Peter Goodrich, Christopher Hardacre, Stefano Passerini, Andrea Balducci, Johan Jacquemin
    Abstract:

    In the scope of improving the energy and power densities of electrochemical double layer capacitors (EDLCs), the development of high performance electrolytes with enhanced operative voltages is imperative. The formulation of mixtures containing ionic liquids with organic molecular solvents is an important strategy in the pursuit of developing highly electrochemically stable and safe materials while retaining fast transport properties for high power applications. In this work, we report on the physical–chemical investigations into binary mixtures containing the ionic liquid 1-butyl-1-methylpyrrolidinium bis{(trifluoromethyl)sulfonyl}imide with one monoNitrile solvent, butyroNitrile, and two diNitrile solvents, glutaroNitrile and adipoNitrile, as potential electrolytes for EDLCs. The thermal, volumetric, and transport properties of the binary mixtures are investigated as functions of the electrolyte composition and temperature. Furthermore, the electrolyte composition which exhibits the highest conductivity for each of the binary mixtures was determined, and its electrochemical stability is reported using a glassy carbon macrodisk electrode.

  • Physical–Chemical Characterization of Binary Mixtures of 1‑Butyl-1-methylpyrrolidinium Bis{(trifluoromethyl)sulfonyl}imide and Aliphatic Nitrile Solvents as Potential Electrolytes for Electrochemical Energy Storage Applications
    2016
    Co-Authors: Alex R Neale, Patrick Wilde, Peter Goodrich, Christopher Hardacre, Stefano Passerini, Andrea Balducci, Christoph Schütter, Johan Jacquemin
    Abstract:

    In the scope of improving the energy and power densities of electrochemical double layer capacitors (EDLCs), the development of high performance electrolytes with enhanced operative voltages is imperative. The formulation of mixtures containing ionic liquids with organic molecular solvents is an important strategy in the pursuit of developing highly electrochemically stable and safe materials while retaining fast transport properties for high power applications. In this work, we report on the physical–chemical investigations into binary mixtures containing the ionic liquid 1-butyl-1-methylpyrrolidinium bis­{(trifluoromethyl)­sulfonyl}­imide with one monoNitrile solvent, butyroNitrile, and two diNitrile solvents, glutaroNitrile and adipoNitrile, as potential electrolytes for EDLCs. The thermal, volumetric, and transport properties of the binary mixtures are investigated as functions of the electrolyte composition and temperature. Furthermore, the electrolyte composition which exhibits the highest conductivity for each of the binary mixtures was determined, and its electrochemical stability is reported using a glassy carbon macrodisk electrode

Jean-luc Dubois - One of the best experts on this subject based on the ideXlab platform.