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Barrie F Taylor - One of the best experts on this subject based on the ideXlab platform.

  • metabolism of methylated osmolytes by aerobic bacteria from mono lake a moderately hypersaline Alkaline Environment
    FEMS Microbiology Ecology, 1996
    Co-Authors: Mara R Diaz, Barrie F Taylor
    Abstract:

    Three strains of aerobic bacteria were isolated from water and sediment samples of Mono Lake, a moderately hypersaline (90 ppt), Alkaline (pH 9.7) lake in California. The organisms, Gram-negative rods, grew fastest at about pH 9.7 with no growth or much slower growth at pH 7.0. All three isolates grew on glycine betaine (GB) and respirometric experiments indicated that catabolism was by sequential demethylation with dimethyl glycine and sarcosine as intermediates. Two of the isolates also grew on dimethylsulfoniopropionate (DMSP), one with cleavage of the DMSP to yield dimethyl sulfide (DMS) and acrylate, and the other by demethylation with 3-methiolpropionate (MMPA) as an intermediate and the production of methanethiol from MMPA. The methylated osmolytes supported growth at salinities similar to those in Mono Lake, but, at higher salinities, catabolism was suppressed and GB and DMSP functioned as osmolytes. GB and DMSP probably originate from cyanobacteria and/or phytoplankton in Mono Lake and this report is the first indication of both the DMS and demethylation/methanethiol-producing pathways for DMSP degradation in a nonmarine Environment.

  • research articlemetabolism of methylated osmolytes by aerobic bacteria from mono lake a moderately hypersaline Alkaline Environment
    FEMS Microbiology Ecology, 1996
    Co-Authors: Mara R Diaz, Barrie F Taylor
    Abstract:

    Three strains of aerobic bacteria were isolated from water and sediment samples of Mono Lake, a moderately hypersaline (90 ppt), Alkaline (pH 9.7) lake in California. The organisms, Gram-negative rods, grew fastest at about pH 9.7 with no growth or much slower growth at pH 7.0. All three isolates grew on glycine betaine (GB) and respirometric experiments indicated that catabolism was by sequential demethylation with dimethyl glycine and sarcosine as intermediates. Two of the isolates also grew on dimethylsulfoniopropionate (DMSP), one with cleavage of the DMSP to yield dimethyl sulfide (DMS) and acrylate, and the other by demethylation with 3-methiolpropionate (MMPA) as an intermediate and the production of methanethiol from MMPA. The methylated osmolytes supported growth at salinities similar to those in Mono Lake, but, at higher salinities, catabolism was suppressed and GB and DMSP functioned as osmolytes. GB and DMSP probably originate from cyanobacteria and/or phytoplankton in Mono Lake and this report is the first indication of both the DMS and demethylation/methanethiol-producing pathways for DMSP degradation in a nonmarine Environment.

Mara R Diaz - One of the best experts on this subject based on the ideXlab platform.

  • metabolism of methylated osmolytes by aerobic bacteria from mono lake a moderately hypersaline Alkaline Environment
    FEMS Microbiology Ecology, 1996
    Co-Authors: Mara R Diaz, Barrie F Taylor
    Abstract:

    Three strains of aerobic bacteria were isolated from water and sediment samples of Mono Lake, a moderately hypersaline (90 ppt), Alkaline (pH 9.7) lake in California. The organisms, Gram-negative rods, grew fastest at about pH 9.7 with no growth or much slower growth at pH 7.0. All three isolates grew on glycine betaine (GB) and respirometric experiments indicated that catabolism was by sequential demethylation with dimethyl glycine and sarcosine as intermediates. Two of the isolates also grew on dimethylsulfoniopropionate (DMSP), one with cleavage of the DMSP to yield dimethyl sulfide (DMS) and acrylate, and the other by demethylation with 3-methiolpropionate (MMPA) as an intermediate and the production of methanethiol from MMPA. The methylated osmolytes supported growth at salinities similar to those in Mono Lake, but, at higher salinities, catabolism was suppressed and GB and DMSP functioned as osmolytes. GB and DMSP probably originate from cyanobacteria and/or phytoplankton in Mono Lake and this report is the first indication of both the DMS and demethylation/methanethiol-producing pathways for DMSP degradation in a nonmarine Environment.

  • research articlemetabolism of methylated osmolytes by aerobic bacteria from mono lake a moderately hypersaline Alkaline Environment
    FEMS Microbiology Ecology, 1996
    Co-Authors: Mara R Diaz, Barrie F Taylor
    Abstract:

    Three strains of aerobic bacteria were isolated from water and sediment samples of Mono Lake, a moderately hypersaline (90 ppt), Alkaline (pH 9.7) lake in California. The organisms, Gram-negative rods, grew fastest at about pH 9.7 with no growth or much slower growth at pH 7.0. All three isolates grew on glycine betaine (GB) and respirometric experiments indicated that catabolism was by sequential demethylation with dimethyl glycine and sarcosine as intermediates. Two of the isolates also grew on dimethylsulfoniopropionate (DMSP), one with cleavage of the DMSP to yield dimethyl sulfide (DMS) and acrylate, and the other by demethylation with 3-methiolpropionate (MMPA) as an intermediate and the production of methanethiol from MMPA. The methylated osmolytes supported growth at salinities similar to those in Mono Lake, but, at higher salinities, catabolism was suppressed and GB and DMSP functioned as osmolytes. GB and DMSP probably originate from cyanobacteria and/or phytoplankton in Mono Lake and this report is the first indication of both the DMS and demethylation/methanethiol-producing pathways for DMSP degradation in a nonmarine Environment.

Baohua Chen - One of the best experts on this subject based on the ideXlab platform.

  • mitogenomic perspectives on the adaptation to extreme Alkaline Environment of amur ide leuciscus waleckii
    Marine Biotechnology, 2020
    Co-Authors: Xiaodi Duan, Chuanju Dong, Laghari Muhammad Younis, Meng Zhang, Baohua Chen
    Abstract:

    Amur ide (Leuciscus waleckii, Family Cyprinidae) is widely distributed in Northeast Asia. L. waleckii usually inhabits freshwater Environments but can also survive in the Lake Dali Nur, one of the most extreme aquatic Environments on the earth, with an alkalinity up to 50 mmol/L (pH 9.6). To investigate mechanisms of mitogenomic evolution underlying adaptation to extreme Environments, we determined 30 complete mitogenomes that included Lake Dali Nur (Alkaline Environment, AL) population and Amur basin (freshwater Environment, FW) population. Through phylogenetic and divergence time analysis, we found that AL and FW populations forming distinct two groups which were consistent with geographic divergence (the formation of Lake Dali Nur). In addition, we found that almost of the windows exhibited higher nucleotide diversity in FW population (avg 0.0046) than AL population (avg 0.0012). This result indicated that severe Environment selection had remarkably reduced the genetic diversity of mitogenome in AL population and suggested that severe Environment selection had remarkably reduced the genetic diversity of mitogenome in the AL population. Compared with the FW population (ω = 0.064), the AL population (ω = 0.092) had a larger mean ω (dN/dS ratios) value for the 13 concatenated mitochondrial protein-coding genes, indicating that the high Alkaline tolerated group had accumulated more nonsynonymous mutations. These nonsynonymous mutations had resulted in slightly beneficial amino acid changes that allowed adaption to the severe conditions. This study provides an additional view to decipher the adaptive mitogenome evolution of L. waleckii of the high Alkaline Environment.

  • genomic basis of adaptive evolution the survival of amur ide leuciscus waleckii in an extremely Alkaline Environment
    Molecular Biology and Evolution, 2017
    Co-Authors: Yanliang Jiang, Wenzhu Peng, Zongli Yao, Baohua Chen, Likun Jiang, Jingyan Feng, Guiming Liu, Zhanjiang Liu, Ruyu Tai
    Abstract:

    The Amur ide (Leuciscus waleckii) is a cyprinid fish that is widely distributed in Northeast Asia. The Lake Dali Nur population inhabits one of the most extreme aquatic Environments on Earth, with an alkalinity up to 50 mmol/L (pH 9.6), thus providing an exceptional model with which to characterize the mechanisms of genomic evolution underlying adaptation to extreme Environments. Here, we developed the reference genome assembly for L. waleckii from Lake Dali Nur. Intriguingly, we identified unusual expanded long terminal repeats (LTRs) with higher nucleotide substitution rates than in many other teleosts, suggesting their more recent insertion into the L. waleckii genome. We also identified expansions in genes encoding egg coat proteins and natriuretic peptide receptors, possibly underlying the adaptation to extreme Environmental stress. We further sequenced the genomes of 10 additional individuals from freshwater and 18 from Lake Dali Nur populations, and we detected a total of 7.6 million SNPs from both populations. In a genome scan and comparison of these two populations, we identified a set of genomic regions under selective sweeps that harbor genes involved in ion homoeostasis, acid-base regulation, unfolded protein response, reactive oxygen species elimination, and urea excretion. Our findings provide comprehensive insight into the genomic mechanisms of teleost fish that underlie their adaptation to extreme Alkaline Environments.

Chenhao Wang - One of the best experts on this subject based on the ideXlab platform.

  • nanostructured cementite ferrous sulfide encapsulated carbon with heteroatoms for oxygen reduction in Alkaline Environment
    ACS Sustainable Chemistry & Engineering, 2019
    Co-Authors: Hsinchih Huang, Kaichin Wang, Hsuehyu Chen, Yuchung Chang, Yenlin Chen, Chenhao Wang
    Abstract:

    Nonprecious metal catalysts of the oxygen reduction reaction (ORR) are highly preferred in anion exchange membrane fuel cells (AEMFCs) because of the high stability and low cost. Here, a novel pyrolyzed poly(3,4-ethylene dioxythiophene) hydrate (PEDOT)-Prussian blue (PB) catalyst (FeCN-S) for the ORR in an AEMFC cathode demonstrated high catalytic performance. After pyrolysis at 800 °C, the templated PB-PEDOT formed the nanostructured Fe3C/FeS encapsulated carbon with heteroatom contribution, which exhibited optimized ORR activity with a direct four-electron transfer pathway for AEMFC applications. This improvement in activity is attributed to the specific structure, the heteroatom contribution, and the coordination structure.

  • Nanostructured Cementite/Ferrous Sulfide Encapsulated Carbon with Heteroatoms for Oxygen Reduction in Alkaline Environment
    2019
    Co-Authors: Hsinchih Huang, Kaichin Wang, Hsuehyu Chen, Yuchung Chang, Yenlin Chen, Chenhao Wang
    Abstract:

    Nonprecious metal catalysts of the oxygen reduction reaction (ORR) are highly preferred in anion exchange membrane fuel cells (AEMFCs) because of the high stability and low cost. Here, a novel pyrolyzed poly­(3,4-ethylene dioxythiophene) hydrate (PEDOT)-Prussian blue (PB) catalyst (FeCN-S) for the ORR in an AEMFC cathode demonstrated high catalytic performance. After pyrolysis at 800 °C, the templated PB-PEDOT formed the nanostructured Fe3C/FeS encapsulated carbon with heteroatom contribution, which exhibited optimized ORR activity with a direct four-electron transfer pathway for AEMFC applications. This improvement in activity is attributed to the specific structure, the heteroatom contribution, and the coordination structure

Chuanju Dong - One of the best experts on this subject based on the ideXlab platform.

  • mitogenomic perspectives on the adaptation to extreme Alkaline Environment of amur ide leuciscus waleckii
    Marine Biotechnology, 2020
    Co-Authors: Xiaodi Duan, Chuanju Dong, Laghari Muhammad Younis, Meng Zhang, Baohua Chen
    Abstract:

    Amur ide (Leuciscus waleckii, Family Cyprinidae) is widely distributed in Northeast Asia. L. waleckii usually inhabits freshwater Environments but can also survive in the Lake Dali Nur, one of the most extreme aquatic Environments on the earth, with an alkalinity up to 50 mmol/L (pH 9.6). To investigate mechanisms of mitogenomic evolution underlying adaptation to extreme Environments, we determined 30 complete mitogenomes that included Lake Dali Nur (Alkaline Environment, AL) population and Amur basin (freshwater Environment, FW) population. Through phylogenetic and divergence time analysis, we found that AL and FW populations forming distinct two groups which were consistent with geographic divergence (the formation of Lake Dali Nur). In addition, we found that almost of the windows exhibited higher nucleotide diversity in FW population (avg 0.0046) than AL population (avg 0.0012). This result indicated that severe Environment selection had remarkably reduced the genetic diversity of mitogenome in AL population and suggested that severe Environment selection had remarkably reduced the genetic diversity of mitogenome in the AL population. Compared with the FW population (ω = 0.064), the AL population (ω = 0.092) had a larger mean ω (dN/dS ratios) value for the 13 concatenated mitochondrial protein-coding genes, indicating that the high Alkaline tolerated group had accumulated more nonsynonymous mutations. These nonsynonymous mutations had resulted in slightly beneficial amino acid changes that allowed adaption to the severe conditions. This study provides an additional view to decipher the adaptive mitogenome evolution of L. waleckii of the high Alkaline Environment.