The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Wesley A Burks - One of the best experts on this subject based on the ideXlab platform.
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soy immunotherapy for peanut Allergic mice modulation of the peanut Allergic Response
The Journal of Allergy and Clinical Immunology, 2004Co-Authors: L Pons, Usha Ponnappan, Renee A Hall, Pippa Simpson, Gael Cockrell, Michael C West, Hugh A Sampson, Ricki M Helm, Wesley A BurksAbstract:Background Allergen-specific immunotherapy (IT) is an effective therapeutic modality to prevent further anaphylactic episodes in patients with insect sting hypersensitivity and is being investigated for peanut allergy. So far, peanut-specific IT has been unsuccessful because of the side effects of therapy. Soybean seed storage proteins share significant homology with the respective peanut allergens. Objective This study was undertaken in mice to investigate whether specific doses of soybean would desensitize peanut-Allergic mice. Methods C3H/HeJ mice were sensitized to peanut with 3 intraperitoneal (IP) injections of crude peanut extract. The mice were desensitized by IP injections with either crude peanut or soybean extract for 4 weeks, 3 times a week. Controls included placebo desensitization with PBS and naive mice. After 2 weeks of rest, mice were challenged IP with crude peanut extract. Thirty minutes later, symptom scores and body temperatures were recorded. Serum immunoglobulins, peanut-induced splenocyte proliferation, and secreted cytokines were measured before and after desensitization. Results The clinical symptoms in the soybean- and peanut-desensitized animals were markedly reduced compared with the placebo-treated mice. Specific IgG1 levels to crude peanut were significantly lower in the soy IT group than in the peanut IT group. The cellular Response to crude peanut was also downregulated in the soy IT group, as shown by decreased peanut-specific stimulation indices and a cytokine profile skewed toward a T H 1 Response. Conclusions Soy IT can be used to desensitize/downregulate peanut-specific Response in peanut-Allergic mice and could provide a new therapeutic intervention for peanut allergy.
Alain Jacquet - One of the best experts on this subject based on the ideXlab platform.
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interactions of airway epithelium with protease allergens in the Allergic Response
Clinical & Experimental Allergy, 2011Co-Authors: Alain JacquetAbstract:Cite this as: A. Jacquet, Clinical & Experimental Allergy, 2011 (41) 305–311. Summary Among the apparently innocuous environmental proteins routinely inhaled by human subjects, only a small proportion of these antigens triggers allergy in susceptible individuals. Although the molecular basis of the allergenicity of these airborne proteins remains to be fully characterized, numerous studies suggest that the ability of such proteins to promote Allergic Responses is at least due to their proteolytic activity. This review will summarize insights into the interactions of protease allergens with the respiratory epithelium. In addition to their capacity to facilitate their antigen presentation through epithelial barrier degradation, protease allergens can directly activate airway mucosal surfaces to recruit inflammatory cells and to initiate the airway remodelling process. A greater understanding of the effects of protease allergens in the airways inflammation as well as on the relevant targets could define novel therapeutic strategies for the treatment Allergic asthma.
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Heat denaturation affects the ProDer p 1 IgE reactivity and downregulates the development of the specific Allergic Response
Journal of Allergy and Clinical Immunology, 2004Co-Authors: Mauro Magi, Lida Garcia, Rémi Palmantier, Michel Vandenbranden, Alain JacquetAbstract:Background Modified allergens with reduced IgE-binding activity represent an elegant approach to circumvent the risk of anaphylactic reactions in allergen-specific immunotherapy. Objective The current work investigated the effect of heat denaturation on the allergenic properties of recombinant ProDer p 1, a precursor form of the major house dust mite allergen Der p 1. Methods The IgE reactivity was estimated by direct and competition ELISA. The immunogenicity of heat-denatured ProDer p 1 was evaluated in naive and Der p 1-Allergic mice. Results Heat denaturation in reducing conditions drastically reduced the in vitro ProDer p 1 IgE reactivity toward human Allergic sera. In naive mice, heat-denatured ProDer p 1 generated mixed TH1-TH2 Responses characterized by the absence of specific IgE with concomitant rise in specific IgG2a titers and the presence of IL-5 and IFN-γ in splenocyte cultures. In contrast, natural Der p 1 or native ProDer p 1 induced typical strict TH2-biased Allergic Responses with strong IgG1 and IgE titers, whereas spleen cells exhibited only high IL-5 secretion. Moreover, native or heat-denatured ProDer p 1 vaccinations prevented airway eosinophil infiltrations after challenge. Although native or heat-treated ProDer p 1 adjuvanted with SBAS1b induced mixed TH1-TH2 Responses in Allergic mice, heat-denatured ProDer p 1, compared with native ProDer p 1, proved to be more effective in redirecting the TH2-Allergic Response toward T H1. Conclusion Taken together, our results suggest that variants of Der p 1 with reduced IgE-binding reactivity could represent hypoallergenic molecules suitable for allergen-specific immunotherapy.
L Pons - One of the best experts on this subject based on the ideXlab platform.
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soy immunotherapy for peanut Allergic mice modulation of the peanut Allergic Response
The Journal of Allergy and Clinical Immunology, 2004Co-Authors: L Pons, Usha Ponnappan, Renee A Hall, Pippa Simpson, Gael Cockrell, Michael C West, Hugh A Sampson, Ricki M Helm, Wesley A BurksAbstract:Background Allergen-specific immunotherapy (IT) is an effective therapeutic modality to prevent further anaphylactic episodes in patients with insect sting hypersensitivity and is being investigated for peanut allergy. So far, peanut-specific IT has been unsuccessful because of the side effects of therapy. Soybean seed storage proteins share significant homology with the respective peanut allergens. Objective This study was undertaken in mice to investigate whether specific doses of soybean would desensitize peanut-Allergic mice. Methods C3H/HeJ mice were sensitized to peanut with 3 intraperitoneal (IP) injections of crude peanut extract. The mice were desensitized by IP injections with either crude peanut or soybean extract for 4 weeks, 3 times a week. Controls included placebo desensitization with PBS and naive mice. After 2 weeks of rest, mice were challenged IP with crude peanut extract. Thirty minutes later, symptom scores and body temperatures were recorded. Serum immunoglobulins, peanut-induced splenocyte proliferation, and secreted cytokines were measured before and after desensitization. Results The clinical symptoms in the soybean- and peanut-desensitized animals were markedly reduced compared with the placebo-treated mice. Specific IgG1 levels to crude peanut were significantly lower in the soy IT group than in the peanut IT group. The cellular Response to crude peanut was also downregulated in the soy IT group, as shown by decreased peanut-specific stimulation indices and a cytokine profile skewed toward a T H 1 Response. Conclusions Soy IT can be used to desensitize/downregulate peanut-specific Response in peanut-Allergic mice and could provide a new therapeutic intervention for peanut allergy.
Marek L Kowalski - One of the best experts on this subject based on the ideXlab platform.
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endocrine disrupters potential modulators of the immune system and Allergic Response
Allergy, 2006Co-Authors: M Chalubinski, Marek L KowalskiAbstract:Endocrine disrupters (EDs) are environmental pollutants of industrial or agricultural origin (e.g. herbicides, fungicides, insecticides, industrial chemicals) that may influence health of wildlife and human. Endocrine-disrupting effect is obtained by mimicking the action of the steroid hormones and has been associated with several reproductive disorders as well as cancerogenesis both in animals and humans. EDs can also influence synthesis of cytokines, immunoglobulins, and cell mediators as well as immune cell activation and survival. Modulation by EDs of interleukin-4 production, Th1/Th2 balance and IgE production suggest their potential effect on Allergic immune Responses. The aim of this review was to summarize data indicating a potential effect of EDs exposure on the immune system and Allergic Responses.
M Kulis - One of the best experts on this subject based on the ideXlab platform.
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a randomized controlled study of peanut oral immunotherapy clinical desensitization and modulation of the Allergic Response
The Journal of Allergy and Clinical Immunology, 2011Co-Authors: Pooja Varshney, Stacie M Jones, Amy M Scurlock, Tamara T Perry, Alex R Kemper, Pamela H Steele, Anne Hiegel, J Kamilaris, S K Carlisle, M KulisAbstract:Background Open-label oral immunotherapy (OIT) protocols have been used to treat small numbers of patients with peanut allergy. Peanut OIT has not been evaluated in double-blind, placebo-controlled trials. Objective To investigate the safety and effectiveness of OIT for peanut allergy in a double-blind, placebo-controlled study. Methods In this multicenter study, children ages 1 to 16 years with peanut allergy received OIT with peanut flour or placebo. Initial escalation, build-up, and maintenance phases were followed by an oral food challenge (OFC) at approximately 1 year. Titrated skin prick tests (SPTs) and laboratory studies were performed at regular intervals. Results Twenty-eight subjects were enrolled in the study. Three peanut OIT subjects withdrew early in the study because of Allergic side effects. During the double-blind, placebo-controlled food challenge, all remaining peanut OIT subjects (n = 16) ingested the maximum cumulative dose of 5000 mg (approximately 20 peanuts), whereas placebo subjects (n = 9) ingested a median cumulative dose of 280 mg (range, 0-1900 mg; P P P = .01), and IL-13 ( P = .02) and increases in peanut-specific IgG 4 ( P P hi : FoxP3 intermediate CD4+ CD25+ T cells increased at the time of OFC ( P = .04) in peanut OIT subjects. Conclusion These results conclusively demonstrate that peanut OIT induces desensitization and concurrent immune modulation. The current study continues and is evaluating the hypothesis that peanut OIT causes long-term immune tolerance.
