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Jing Gung Chung - One of the best experts on this subject based on the ideXlab platform.

  • Aloe emodin induces cell death through s phase arrest and caspase dependent pathways in human tongue squamous cancer scc 4 cells
    Anticancer Research, 2009
    Co-Authors: Tsan Hung Chiu, Te Chun Hsia, Jai Sing Yang, Ping Ping Wu, Chia Yu, Chin Chin Ho, Hsu Feng Lu, Gibson W Wood, Jing Gung Chung
    Abstract:

    Aloe-emodin, one of the anthraquinones, has been shown to have anticancer activity in different kinds of human cancer cell lines. Therefore, the purpose of this study was to investigate the anti-cancer effect of Aloe-emodin on human tongue squamous carcinoma SCC-4 cells. The results indicated that Aloe-emodin induced cell death through S-phase arrest and apoptosis in a dose- and time-dependent manner. Treatment with 30 μM of Aloe-emodin led to S-phase arrest through promoted p53, p21 and p27, but inhibited cyclin A, E, thymidylate synthase and Cdc25A levels. Aloe-emodin promoted the release of apoptosis-inducing factor (AIF), endonuclease G (Endo G), pro-caspase-9 and cytochrome c from the mitochondria via a loss of the mitochondrial membrane potential (ΔΨ m ) which was associated with a increase in the ratio of B-cell lymphoma 2-associated X protein (Bax)/B cell lymphoma/leukemia-2 (Bcl-2) and activation of caspase-9 and -3. The free radical scavenger N- acetylcysteine (NAC) and caspase inhibitors markedly blocked Aloe-emodin-induced apoptosis. Aloe-emodin thus induced apoptosis in the SCC-4 cells through the Fas/death-receptor, mitochondria and caspase cascade. Aloe-emodin could be a novel chemotherapeutic drug candidate for the treatment of human tongue squamous cancer in the future.

  • Aloe emodin induces cell death through s phase arrest and caspase dependent pathways in human tongue squamous cancer scc 4 cells
    Anticancer Research, 2009
    Co-Authors: Tsan Hung Chiu, Tung Yuan Lai, Te Chun Hsia, Jai Sing Yang, Gibson W Wood, Wan Wen Lai, Chin Chung Yeh, Jing Gung Chung
    Abstract:

    Aloe-emodin, one of the anthraquinones, has been shown to have anticancer activity in different kinds of human cancer cell lines. Therefore, the purpose of this study was to investigate the anti-cancer effect of Aloe-emodin on human tongue squamous carcinoma SCC-4 cells. The results indicated that Aloe-emodin induced cell death through S-phase arrest and apoptosis in a dose- and time-dependent manner. Treatment with 30 microM of Aloe-emodin led to S-phase arrest through promoted p53, p21 and p27, but inhibited cyclin A, E, thymidylate synthase and Cdc25A levels. Aloe-emodin promoted the release of apoptosis-inducing factor (AIF), endonuclease G (Endo G), pro-caspase-9 and cytochrome c from the mitochondria via a loss of the mitochondrial membrane potential (DeltaPsi(m)) which was associated with a increase in the ratio of B-cell lymphoma 2-associated X protein (Bax)/B cell lymphoma/leukemia-2 (Bcl-2) and activation of caspase-9 and -3. The free radical scavenger N-acetylcysteine (NAC) and caspase inhibitors markedly blocked Aloe-emodin-induced apoptosis. Aloe-emodin thus induced apoptosis in the SCC-4 cells through the Fas/death-receptor, mitochondria and caspase cascade. Aloe-emodin could be a novel chemotherapeutic drug candidate for the treatment of human tongue squamous cancer in the future.

  • Aloe emodin induced in vitro g2 m arrest of cell cycle in human promyelocytic leukemia hl 60 cells
    Food and Chemical Toxicology, 2004
    Co-Authors: Hsin-chun Chen, Wen Tsong Hsieh, W. C. Chang, Jing Gung Chung
    Abstract:

    Abstract In this study, we have evaluated the chemopreventive role of Aloe-emodin in human promyelocytic leukemia HL-60 cells in vitro by studying the regulation of proliferation, cell cycle and apoptosis. Aloe-emodin inhibited cell proliferation and induced G2/M arrest and apoptosis in HL-60 cells. Investigation of the levels of cyclins B1, E and A by immunoblot analysis showed that cyclin E level was unaffected, whereas cyclin B1 and A levels increased with Aloe-emodin in HL-60 cells. Investigation of the levels of cyclin-dependent kinases, Cdk1 and 2, showed increased levels of Cdk1 but the levels of Cdk2 were not effected with Aloe-emodin in HL-60 cells. The levels of p27 were increased after HL-60 cells were cotreated with various concentrations of Aloe-emodin. The increase of the levels of p27 may be the major factor for Aloe-emodin to cause G2/M arrest in these examined cells. Flow cytometric assays and DNA fragmentation gel electrophoresis also confirmed Aloe-emodin induced apoptosis in HL-60 cells. The levels of caspase-3 were increased after HL-60 cells were cotreated with 10 μM Aloe-emodin for 12, 24, 48, and 72 hours. Taken together, Aloe-emodin therefore appears to exert its anticarcinogenesis properties by inhibiting proliferation and inducing cell cycle arrest and apoptosis underwent activation of caspase-3 in human leukemia HL-60 cells.

Frederick A Beland - One of the best experts on this subject based on the ideXlab platform.

  • an evaluation of the biological and toxicological properties of Aloe barbadensis miller Aloe vera
    Journal of Environmental Science and Health Part C-environmental Carcinogenesis & Ecotoxicology Reviews, 2006
    Co-Authors: Frederick A Beland
    Abstract:

    Aloe barbadensis (Miller), Aloe vera, has a long history of use as a topical and oral therapeutic. The plant is the source of two products, gel and latex, which are obtained from its fleshy leaves. Aloe vera products contain multiple constituents with potential biological and toxicological activities, yet the active components elude definition. Ingestion of Aloe vera is associated with diarrhea, electrolyte imbalance, kidney dysfunction, and conventional drug interactions; episodes of contact dermatitis, erythema, and phototoxicity have been reported from topical applications. This review examines the botany, physical and chemical properties, and biological activities of the Aloe vera plant.

Mei-chun Liu - One of the best experts on this subject based on the ideXlab platform.

  • Effects and mechanisms of Aloe-emodin on cell death in human lung squamous cell carcinoma
    European journal of pharmacology, 2001
    Co-Authors: Hong-zin Lee, Shih-lan Hsu, Mei-chun Liu
    Abstract:

    Aloe-emodin (1,8-dihydroxy-3-(hydroxymethyl)-anthraquinone) is an active component from the root and rhizome of Rheum palmatum. The study investigated the effects and mechanisms of Aloe-emodin-induced cell death in human lung squamous cell carcinoma cell line CH27. Aloe-emodin (40 microM)-induced CH27 cell apoptosis was confirmed by DNA fragmentation (DNA ladders and sub-G(1) formation). Aloe-emodin-induced apoptosis of CH27 cells involved modulation of the expression of Bcl-2 family proteins, such as BclX(L), Bag-1, and Bak, and was associated with the translocation of Bak and Bax from cytosolic to particulate fractions. Aloe-emodin-treated CH27 cells had an increased relative abundance of cytochrome c in the cytosolic fraction. Results demonstrated that the activation of caspase-3, caspase-8, and caspase-9 is an important determinant of apoptotic death induced by Aloe-emodin. These results suggest that Aloe-emodin induces CH27 cell death by the Bax and Fas death pathway.

Z V P Murthy - One of the best experts on this subject based on the ideXlab platform.

  • rheology of Aloe barbadensis miller a naturally available material of high therapeutic and nutrient value for food applications
    Journal of Food Engineering, 2013
    Co-Authors: V N Lad, Z V P Murthy
    Abstract:

    Abstract Due to the potential applications of Aloe barbadensis Miller (Aloe vera) in cosmetics, pharmaceutical and food products, the study of its rheology is important. Recently, many commercial food-product producers have boosted up the usage of Aloe vera gel or juice in one form or the other due to its nutrient ingredients. The present study reports the rheological characteristics of native Aloe gel and juice under dynamic and steady shear. The damping of the elastic moduli and viscous moduli at various temperatures for the Aloe gel under oscillatory shear tests have been reported, for the first time, which were observed due to the presence of weak, fibrous and random structure of polysaccharides in it. The moduli for gel increased with increasing temperature and that for juice decreased with temperature. Prior to attaining the plateau region after certain shear rate, Aloe vera gel and juice exhibited shear thinning behavior. The flow behavior index for Aloe gel samples was found to be 0.1 in the shear thinning region.

Marcia Rp Oliveira - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of wound healing effect of alginate films containing Aloe vera Aloe barbadensis miller gel
    Journal of Biomaterials Applications, 2018
    Co-Authors: Adriana Yuriko Koga, Airton Vicente Pereira, Leandro Cavalcante Lipinski, Marcia Rp Oliveira
    Abstract:

    A new alginate dressing with Aloe vera gel was developed and used to study its cutaneous healing effect in vivo by surgical wounds in Wistar rats. The Aloe-alginate film was characterized by Fourier-transform infrared spectroscopy, mechanical properties, scanning electron microscopy, hydration rate, and calcium release. The Aloe-alginate film demonstrated desirable physical and mechanical characteristics for wound dressing application. The animals were divided in three groups (n = 20): gauze bandage, alginate film, and Aloe-alginate film. The histological analysis on the 3rd, 7th, 14th, and 22nd days after the treatment showed that the Aloe-alginate films modulated the inflammatory phase and decreased the quantity of macrophages when compared to the alginate film group and the control (gauze bandage). The evaluation of collagen fibers showed a decrease in type III and increase in type I fibers on group treated with Aloe-alginate film suggesting an improvement in the progression of the healing process.