The Experts below are selected from a list of 45 Experts worldwide ranked by ideXlab platform
Raffaele Antonelli Incalzi - One of the best experts on this subject based on the ideXlab platform.
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Alpha 1 Globulin fraction of serum protein electrophoresis for screening and early detection of Alpha 1 antitrypsin deficiency
European Respiratory Journal, 2018Co-Authors: Simone Scarlata, Simona Santangelo, Panaiotis Finamore, Gilda Giannunzio, Davide Fontana, Raffaele Antonelli IncalziAbstract:Alpha 1 antitrypsin deficiency (AATD) is a relevant underdiagnosed disease. Although the quantitative determination of AAT levels in blood is currently stated as the pivotal first test to identify AATD, it is often underused. We performed an integrative diagnostic algorithm to predict the presence of A1AT variants on phenotyping, genotyping or direct sequencing. We retrospectively analyzed serum protein electrophoresis, liver transaminases, from 31,905 subjects collected at the “Campus Biomedico” University Hospital between 2008 and 2016 over a referral population of 412,836 people. Results obtained from this administrative data set were then compared with those prospectively coming from a standard screening approach at the Respiratory outpatient clinic of the same hospital taken between 2016 and 2017. We cross-matched subjects according to the following four classification criteria: - Alpha 1 Globulin≤2.6% (Group A) - Serum A1AT levels - Alpha 1 Globulin≤2.9% and AST/GOT:>37 U/L and ALT/GPT:>78 U/L (Group C); - Alpha 1 Globulin%: 2,90-4,90% and ESR>34 mm/h and CRP>3 mg/L and AST:>37 U/L and ALT:>78 U/L (Group D) We found that PI M variant prevalence rates (cohort: N°/size) was 31/21094=0.00147 (below the observed one in northern Europe – 0.00181 and southern Europe – 0.00228). The crude detection rate within the screened group was 47% versus a detection rate in the control group of outpatient patients of 12%. In conclusion, large scale serum protein electrophoresis and alha 1 Globulin evaluation could represent a valuable approach for early detection of subjects with A1ATD.
Simone Scarlata - One of the best experts on this subject based on the ideXlab platform.
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Alpha 1 Globulin fraction of serum protein electrophoresis for screening and early detection of Alpha 1 antitrypsin deficiency
European Respiratory Journal, 2018Co-Authors: Simone Scarlata, Simona Santangelo, Panaiotis Finamore, Gilda Giannunzio, Davide Fontana, Raffaele Antonelli IncalziAbstract:Alpha 1 antitrypsin deficiency (AATD) is a relevant underdiagnosed disease. Although the quantitative determination of AAT levels in blood is currently stated as the pivotal first test to identify AATD, it is often underused. We performed an integrative diagnostic algorithm to predict the presence of A1AT variants on phenotyping, genotyping or direct sequencing. We retrospectively analyzed serum protein electrophoresis, liver transaminases, from 31,905 subjects collected at the “Campus Biomedico” University Hospital between 2008 and 2016 over a referral population of 412,836 people. Results obtained from this administrative data set were then compared with those prospectively coming from a standard screening approach at the Respiratory outpatient clinic of the same hospital taken between 2016 and 2017. We cross-matched subjects according to the following four classification criteria: - Alpha 1 Globulin≤2.6% (Group A) - Serum A1AT levels - Alpha 1 Globulin≤2.9% and AST/GOT:>37 U/L and ALT/GPT:>78 U/L (Group C); - Alpha 1 Globulin%: 2,90-4,90% and ESR>34 mm/h and CRP>3 mg/L and AST:>37 U/L and ALT:>78 U/L (Group D) We found that PI M variant prevalence rates (cohort: N°/size) was 31/21094=0.00147 (below the observed one in northern Europe – 0.00181 and southern Europe – 0.00228). The crude detection rate within the screened group was 47% versus a detection rate in the control group of outpatient patients of 12%. In conclusion, large scale serum protein electrophoresis and alha 1 Globulin evaluation could represent a valuable approach for early detection of subjects with A1ATD.
Patrick Stordeur - One of the best experts on this subject based on the ideXlab platform.
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protein and enzyme patterns in the fluid cavities of the first trimester gestational sac relevance to the absorptive role of secondary yolk sac
Molecular Human Reproduction, 1998Co-Authors: Beatrice Gulbis, Eric Jauniaux, Frederic Cotton, Patrick StordeurAbstract:The potential absorptive role of the yolk sac membrane was evaluated by examining protein and enzyme patterns in embryonic fluids and by comparing the synthetic capacity of the secondary yolk sec, fetal liver and placenta for human chorionic gonadotrophin (HCG) and Alpha-fetoprotein (Alpha FP). In yolk sac fluid samples, protein electrophoresis showed two main electrophoretic bands with mobilities comparable to those of albumin and interalbumin-Alpha 1-Globulin, and immunoblotting revealed the presence of albumin, Alpha FP, Alpha 1-antitrypsin, Alpha 2-macroGlobulin. transferrin, complement factors 3 and 4 and immunoGlobulin G. In coelomic fluid, similar results were obtained, except for the absence of Alpha 2-macroGlobulin and the presence of ceruloplasmin and IgA, After electrophoresis and immunoblotting with specific antibodies, beta-HCG was detected in all placental homogenates and culture media but was not revealed in any of the corresponding yolk sec tissue samples. Reverse transcription-polymerase chain reaction (RT-PCR) showed that all placental samples express beta-HCG mRNA whereas all yolk sec and liver samples express Alpha FP mRNA. These findings suggest that the yolk sec membrane is an important zone of transfer between the extra-embryonic and embryonic compartments and may also help to further develop therapeutic protocols making use of fetal somatic gene therapy by injecting transduced cells into the exocoelomic cavity.
Yu-ju Lin - One of the best experts on this subject based on the ideXlab platform.
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Predictability of Liver-Related Seromarkers for the Risk of Hepatocellular Carcinoma in Chronic Hepatitis B Patients
2014Co-Authors: Yu-ju LinAbstract:Background: Hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) is a major global health problem. A few risk calculators have been developed using mainly HBV seromarkers as predictors. However, serum HBV DNA level, HBV genotype, and mutants are not routinely checked in regular health examinations. This study aimed to assess the predictability of HCC risk in chronic hepatitis B patients, using a combination of liver-related seromarkers combined with or without HBV seromarkers. Methods: A prospective cohort of 1,822 anti-HCV-seronegative chronic HBV carriers was included in this study. Liver-related seromarkers including aspartate aminotransferase (AST), alanine aminotransferase (ALT), Alpha-fetoprotein (AFP), gamma-glutamyltransferase (GGT), total bilirubin, total protein, albumin, serum Globulins, apolipoprotein A1, and apolipoprotein B were examined. Hazard ratios of HCC with 95% confidence intervals were estimated using Cox proportional hazards regression models. Regression coefficients of seromarkers significantly associated with HCC risk in multivariate analyses were used to create integer risk scores. The predictability of various risk models were assessed by area under receiver operating characteristic curves (AUROCs). Results: During a median follow-up of 5.9 years, 48 newly-developed HCC cases were ascertained. Elevated serum levels of ALT (>= 28 U/L), AFP (>= 5 ng/mL), and GGT (>= 41 U/L), an increased AST/ALT ratio (AAR, >= 1), and lowered serum levels of albumin (<= 4.1 g/dL) and Alpha-1 Globulin (<= 0.2 g/dL) were significantly associated with an increased HCC risk (P<0.05) in multivariate analysis. The risk model incorporating age, gender, AAR, and serum levels of ALT, AFP, GGT, albumin, and Alpha-1 Globulin had an AUROC of 0.89 for predicting 6-year HCC incidence. The AUROC was 0.91 after the addition of HBV seromarkers into the model, and 0.83 for the model without liver-related seromarkers, with the exception of ALT. Conclusion: Liver-related seromarkers may be combined into useful risk models for predicting HBV-related HCC risk
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Predictability of liver-related seromarkers for the risk of hepatocellular carcinoma in chronic hepatitis B patients.
Public Library of Science (PLoS), 1Co-Authors: Yu-ju Lin, Mei-hsuan Lee, Hwai-i Yang, Chin-lan Jen, San-lin You, Li-yu Wang, Jessica Liu, Chien-jen ChenAbstract:BACKGROUND: Hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) is a major global health problem. A few risk calculators have been developed using mainly HBV seromarkers as predictors. However, serum HBV DNA level, HBV genotype, and mutants are not routinely checked in regular health examinations. This study aimed to assess the predictability of HCC risk in chronic hepatitis B patients, using a combination of liver-related seromarkers combined with or without HBV seromarkers. METHODS: A prospective cohort of 1,822 anti-HCV-seronegative chronic HBV carriers was included in this study. Liver-related seromarkers including aspartate aminotransferase (AST), alanine aminotransferase (ALT), Alpha-fetoprotein (AFP), gamma-glutamyltransferase (GGT), total bilirubin, total protein, albumin, serum Globulins, apolipoprotein A1, and apolipoprotein B were examined. Hazard ratios of HCC with 95% confidence intervals were estimated using Cox proportional hazards regression models. Regression coefficients of seromarkers significantly associated with HCC risk in multivariate analyses were used to create integer risk scores. The predictability of various risk models were assessed by area under receiver operating characteristic curves (AUROCs). RESULTS: During a median follow-up of 5.9 years, 48 newly-developed HCC cases were ascertained. Elevated serum levels of ALT (≥ 28 U/L), AFP (≥ 5 ng/mL), and GGT (≥ 41 U/L), an increased AST/ALT ratio (AAR, ≥ 1), and lowered serum levels of albumin (≤ 4.1 g/dL) and Alpha-1 Globulin (≤ 0.2 g/dL) were significantly associated with an increased HCC risk (P
Simona Santangelo - One of the best experts on this subject based on the ideXlab platform.
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Alpha 1 Globulin fraction of serum protein electrophoresis for screening and early detection of Alpha 1 antitrypsin deficiency
European Respiratory Journal, 2018Co-Authors: Simone Scarlata, Simona Santangelo, Panaiotis Finamore, Gilda Giannunzio, Davide Fontana, Raffaele Antonelli IncalziAbstract:Alpha 1 antitrypsin deficiency (AATD) is a relevant underdiagnosed disease. Although the quantitative determination of AAT levels in blood is currently stated as the pivotal first test to identify AATD, it is often underused. We performed an integrative diagnostic algorithm to predict the presence of A1AT variants on phenotyping, genotyping or direct sequencing. We retrospectively analyzed serum protein electrophoresis, liver transaminases, from 31,905 subjects collected at the “Campus Biomedico” University Hospital between 2008 and 2016 over a referral population of 412,836 people. Results obtained from this administrative data set were then compared with those prospectively coming from a standard screening approach at the Respiratory outpatient clinic of the same hospital taken between 2016 and 2017. We cross-matched subjects according to the following four classification criteria: - Alpha 1 Globulin≤2.6% (Group A) - Serum A1AT levels - Alpha 1 Globulin≤2.9% and AST/GOT:>37 U/L and ALT/GPT:>78 U/L (Group C); - Alpha 1 Globulin%: 2,90-4,90% and ESR>34 mm/h and CRP>3 mg/L and AST:>37 U/L and ALT:>78 U/L (Group D) We found that PI M variant prevalence rates (cohort: N°/size) was 31/21094=0.00147 (below the observed one in northern Europe – 0.00181 and southern Europe – 0.00228). The crude detection rate within the screened group was 47% versus a detection rate in the control group of outpatient patients of 12%. In conclusion, large scale serum protein electrophoresis and alha 1 Globulin evaluation could represent a valuable approach for early detection of subjects with A1ATD.