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William T Frankenberger - One of the best experts on this subject based on the ideXlab platform.
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biodegradation of gamma Hexachlorocyclohexane lindane and Alpha Hexachlorocyclohexane in water and a soil slurry by a pandoraea species
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Benedict C Okeke, Tariq Siddique, Marta Camps Arbestain, William T FrankenbergerAbstract:Isomers of 1,2,3,4,5,6-Hexachlorocyclohexane (HCH) were some of the most widely used pesticides. Despite reduction in their production and use, HCH isomers present a serious environmental hazard. In this study, two bacterial isolates (LIN-1 and LIN-3) that can grow on gamma-HCH as a sole source of carbon and energy were isolated from an enrichment culture. In liquid cultures of LIN-1 and LIN-3, 25.0 and 45.5% removal of gamma-HCH, respectively, were achieved in 2 weeks. LIN-3 was identified as Pandoraea sp. by 16S rRNA gene sequence analysis (99% identity). Pandoraea sp. substantially degraded both gamma- and Alpha-HCH isomers at concentrations of 10-200 mg L(-1) in liquid cultures. After 8 weeks of incubation in liquid culture, 89.9 and 93.3% of the gamma- and Alpha-HCH isomers declined, respectively, at an initial concentration of 150 mg L(-1). In soil slurry cultures of Pandoraea sp., simulating a soil slurry phase bioremediation treatment, substantial decreases in the levels of the HCH isomers were observed at concentrations of 50-200 mg L(-1). After 9 weeks, 59.6 and 53.3% biodegradations of gamma- and Alpha-HCH isomers, respectively, were achieved at 150 mg L(-1). Using two 23-mer oligonucloetide primers targeting the 330 bp region of the 16S rRNA gene of Pandoraea sp., an approximately 330 bp PCR product was successfully amplified from DNA templates prepared from bacterial colonies and soil slurry culture. This system provides a direct and rapid PCR-based molecular tool for tracking Pandoraea sp. strain LIN-3 in water and soils. These results have implied implications for the treatment of soils and water contaminated with HCH isomers.
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biodegradation of gamma Hexachlorocyclohexane lindane and Alpha Hexachlorocyclohexane in water and a soil slurry by a pandoraea species
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Benedict C Okeke, Tariq Siddique, Marta Camps Arbestain, William T FrankenbergerAbstract:Isomers of 1,2,3,4,5,6-Hexachlorocyclohexane (HCH) were some of the most widely used pesticides. Despite reduction in their production and use, HCH isomers present a serious environmental hazard. In this study, two bacterial isolates (LIN-1 and LIN-3) that can grow on γ-HCH as a sole source of carbon and energy were isolated from an enrichment culture. In liquid cultures of LIN-1 and LIN-3, 25.0 and 45.5% removal of γ-HCH, respectively, were achieved in 2 weeks. LIN-3 was identified as Pandoraea sp. by 16S rRNA gene sequence analysis (99% identity). Pandoraea sp. substantially degraded both γ- and α-HCH isomers at concentrations of 10−200 mg L-1 in liquid cultures. After 8 weeks of incubation in liquid culture, 89.9 and 93.3% of the γ- and α-HCH isomers declined, respectively, at an initial concentration of 150 mg L-1. In soil slurry cultures of Pandoraea sp., simulating a soil slurry phase bioremediation treatment, substantial decreases in the levels of the HCH isomers were observed at concentrations of ...
Benedict C Okeke - One of the best experts on this subject based on the ideXlab platform.
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biodegradation of gamma Hexachlorocyclohexane lindane and Alpha Hexachlorocyclohexane in water and a soil slurry by a pandoraea species
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Benedict C Okeke, Tariq Siddique, Marta Camps Arbestain, William T FrankenbergerAbstract:Isomers of 1,2,3,4,5,6-Hexachlorocyclohexane (HCH) were some of the most widely used pesticides. Despite reduction in their production and use, HCH isomers present a serious environmental hazard. In this study, two bacterial isolates (LIN-1 and LIN-3) that can grow on gamma-HCH as a sole source of carbon and energy were isolated from an enrichment culture. In liquid cultures of LIN-1 and LIN-3, 25.0 and 45.5% removal of gamma-HCH, respectively, were achieved in 2 weeks. LIN-3 was identified as Pandoraea sp. by 16S rRNA gene sequence analysis (99% identity). Pandoraea sp. substantially degraded both gamma- and Alpha-HCH isomers at concentrations of 10-200 mg L(-1) in liquid cultures. After 8 weeks of incubation in liquid culture, 89.9 and 93.3% of the gamma- and Alpha-HCH isomers declined, respectively, at an initial concentration of 150 mg L(-1). In soil slurry cultures of Pandoraea sp., simulating a soil slurry phase bioremediation treatment, substantial decreases in the levels of the HCH isomers were observed at concentrations of 50-200 mg L(-1). After 9 weeks, 59.6 and 53.3% biodegradations of gamma- and Alpha-HCH isomers, respectively, were achieved at 150 mg L(-1). Using two 23-mer oligonucloetide primers targeting the 330 bp region of the 16S rRNA gene of Pandoraea sp., an approximately 330 bp PCR product was successfully amplified from DNA templates prepared from bacterial colonies and soil slurry culture. This system provides a direct and rapid PCR-based molecular tool for tracking Pandoraea sp. strain LIN-3 in water and soils. These results have implied implications for the treatment of soils and water contaminated with HCH isomers.
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biodegradation of gamma Hexachlorocyclohexane lindane and Alpha Hexachlorocyclohexane in water and a soil slurry by a pandoraea species
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Benedict C Okeke, Tariq Siddique, Marta Camps Arbestain, William T FrankenbergerAbstract:Isomers of 1,2,3,4,5,6-Hexachlorocyclohexane (HCH) were some of the most widely used pesticides. Despite reduction in their production and use, HCH isomers present a serious environmental hazard. In this study, two bacterial isolates (LIN-1 and LIN-3) that can grow on γ-HCH as a sole source of carbon and energy were isolated from an enrichment culture. In liquid cultures of LIN-1 and LIN-3, 25.0 and 45.5% removal of γ-HCH, respectively, were achieved in 2 weeks. LIN-3 was identified as Pandoraea sp. by 16S rRNA gene sequence analysis (99% identity). Pandoraea sp. substantially degraded both γ- and α-HCH isomers at concentrations of 10−200 mg L-1 in liquid cultures. After 8 weeks of incubation in liquid culture, 89.9 and 93.3% of the γ- and α-HCH isomers declined, respectively, at an initial concentration of 150 mg L-1. In soil slurry cultures of Pandoraea sp., simulating a soil slurry phase bioremediation treatment, substantial decreases in the levels of the HCH isomers were observed at concentrations of ...
Howsam Mike - One of the best experts on this subject based on the ideXlab platform.
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Organochlorine exposure and colorectal cancer risk
'Environmental Health Perspectives', 2019Co-Authors: Howsam Mike, Grimalt, Joan O., Guinó Elisabet, Navarro Matilde, Martí Ragué J., Peinado Morales, Miguel Á. Ángel), Capellá G., Moreno Aguado Víctor, Bellvitge Colorectal Cancer GroupAbstract:Organochlorine compounds have been linked to increased risk of several cancers. Despite reductions in their use and fugitive release, they remain one of the most important groups of persistent pollutants to which humans are exposed, primarily through dietary intake. We designed a case-control study to assess the risk of colorectal cancer with exposure to these chemicals, and their potential interactions with genetic alterations in the tumors. A subsample of cases (n = 132) and hospital controls (n = 76) was selected from a larger case-control study in Barcelona, Catalonia, Spain. We measured concentrations in serum of several organochlorines by gas chromatography. We assessed point mutations in K-ras and p53 genes in tissue samples by polymerase chain reaction/single-strand conformation polymorphism and assessed expression of p53 protein by immunohistochemical methods. An elevated risk of colorectal cancer was associated with higher serum concentrations of mono-ortho polychlorinated biphenyl (PCB) congeners 28 and 118. The odds ratio for these mono-ortho PCBs for middle and higher tertile were, respectively, 1.82 [95% confidence interval (CI), 0.90-3.70] and 2.94 (95% CI, 1.39-6.20). Alpha-Hexachlorocyclohexane, hexachlorobenzene, and pp'-DDE (4,4'-dichlorodiphenyltrichloroethene) showed nonsignificant increases in risk. Risk associated with mono-ortho PCBs was slightly higher for tumors with mutations in the p53 gene but was not modified by mutations in K-ras. Mono-ortho PCBs were further associated with transversion-type mutations in both genes. These results generate the hypothesis that exposure to mono-ortho PCBs contributes to human colorectal cancer development. The trend and magnitude of the association, as well as the observation of a molecular fingerprint in tumors, raise the possibility that this finding may be causal
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Organochlorine exposure and colorectal cancer risk
'Environmental Health Perspectives', 2004Co-Authors: Howsam Mike, Grimalt, Joan O., Guinó Elisabet, Navarro Matilde, Martí-ragué Juan, Peinado, Miguel A., Capellá GabrielAbstract:7 pages, 6 tables.-- PMID: 15531428 [PubMed].-- PMCID: PMC1247607.-- Printed version published Nov 2004.Organochlorine compounds have been linked to increased risk of several cancers. Despite reductions in their use and fugitive release, they remain one of the most important groups of persistent pollutants to which humans are exposed, primarily through dietary intake. We designed a case-control study to assess the risk of colorectal cancer with exposure to these chemicals, and their potential interactions with genetic alterations in the tumors. A subsample of cases (n = 132) and hospital controls (n = 76) was selected from a larger case-control study in Barcelona, Catalonia, Spain. We measured concentrations in serum of several organochlorines by gas chromatography. We assessed point mutations in K-ras and p53 genes in tissue samples by polymerase chain reaction/single-strand conformation polymorphism and assessed expression of p53 protein by immunohistochemical methods. An elevated risk of colorectal cancer was associated with higher serum concentrations of mono-ortho polychlorinated biphenyl (PCB) congeners 28 and 118. The odds ratio for these mono-ortho PCBs for middle and higher tertile were, respectively, 1.82 [95% confidence interval (CI) , 0.90-3.70] and 2.94 (95% CI, 1.39-6.20) . Alpha-Hexachlorocyclohexane, hexachlorobenzene, and p,p´-DDE (4,4´-dichlorodiphenyltrichloroethene) showed nonsignificant increases in risk. Risk associated with mono-ortho PCBs was slightly higher for tumors with mutations in the p53 gene but was not modified by mutations in K-ras. Mono-ortho PCBs were further associated with transversion-type mutations in both genes. These results generate the hypothesis that exposure to mono-ortho PCBs contributes to human colorectal cancer development. The trend and magnitude of the association, as well as the observation of a molecular fingerprint in tumors, raise the possibility that this finding may be causal.The study was supported by grants from the Fundació La Marató de TV3 (48/95), the Spanish Fondo de Investigaciones Sanitarias (FIS 96/0797, FIS 00/0027, FIS 01/1264, FIS 03/0114, ISCIII, Red de Centros RCESP C03/09, and Red de Centros de Cancer C03/10) and Comisión Interministerial de Ciencia y Tecnología (SAF 00/81), and a European Union Marie Curie Fellowship for M.H. (HPMFCT 2000-00888).Peer reviewe
Marta Camps Arbestain - One of the best experts on this subject based on the ideXlab platform.
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biodegradation of gamma Hexachlorocyclohexane lindane and Alpha Hexachlorocyclohexane in water and a soil slurry by a pandoraea species
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Benedict C Okeke, Tariq Siddique, Marta Camps Arbestain, William T FrankenbergerAbstract:Isomers of 1,2,3,4,5,6-Hexachlorocyclohexane (HCH) were some of the most widely used pesticides. Despite reduction in their production and use, HCH isomers present a serious environmental hazard. In this study, two bacterial isolates (LIN-1 and LIN-3) that can grow on gamma-HCH as a sole source of carbon and energy were isolated from an enrichment culture. In liquid cultures of LIN-1 and LIN-3, 25.0 and 45.5% removal of gamma-HCH, respectively, were achieved in 2 weeks. LIN-3 was identified as Pandoraea sp. by 16S rRNA gene sequence analysis (99% identity). Pandoraea sp. substantially degraded both gamma- and Alpha-HCH isomers at concentrations of 10-200 mg L(-1) in liquid cultures. After 8 weeks of incubation in liquid culture, 89.9 and 93.3% of the gamma- and Alpha-HCH isomers declined, respectively, at an initial concentration of 150 mg L(-1). In soil slurry cultures of Pandoraea sp., simulating a soil slurry phase bioremediation treatment, substantial decreases in the levels of the HCH isomers were observed at concentrations of 50-200 mg L(-1). After 9 weeks, 59.6 and 53.3% biodegradations of gamma- and Alpha-HCH isomers, respectively, were achieved at 150 mg L(-1). Using two 23-mer oligonucloetide primers targeting the 330 bp region of the 16S rRNA gene of Pandoraea sp., an approximately 330 bp PCR product was successfully amplified from DNA templates prepared from bacterial colonies and soil slurry culture. This system provides a direct and rapid PCR-based molecular tool for tracking Pandoraea sp. strain LIN-3 in water and soils. These results have implied implications for the treatment of soils and water contaminated with HCH isomers.
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biodegradation of gamma Hexachlorocyclohexane lindane and Alpha Hexachlorocyclohexane in water and a soil slurry by a pandoraea species
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Benedict C Okeke, Tariq Siddique, Marta Camps Arbestain, William T FrankenbergerAbstract:Isomers of 1,2,3,4,5,6-Hexachlorocyclohexane (HCH) were some of the most widely used pesticides. Despite reduction in their production and use, HCH isomers present a serious environmental hazard. In this study, two bacterial isolates (LIN-1 and LIN-3) that can grow on γ-HCH as a sole source of carbon and energy were isolated from an enrichment culture. In liquid cultures of LIN-1 and LIN-3, 25.0 and 45.5% removal of γ-HCH, respectively, were achieved in 2 weeks. LIN-3 was identified as Pandoraea sp. by 16S rRNA gene sequence analysis (99% identity). Pandoraea sp. substantially degraded both γ- and α-HCH isomers at concentrations of 10−200 mg L-1 in liquid cultures. After 8 weeks of incubation in liquid culture, 89.9 and 93.3% of the γ- and α-HCH isomers declined, respectively, at an initial concentration of 150 mg L-1. In soil slurry cultures of Pandoraea sp., simulating a soil slurry phase bioremediation treatment, substantial decreases in the levels of the HCH isomers were observed at concentrations of ...
Tariq Siddique - One of the best experts on this subject based on the ideXlab platform.
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biodegradation of gamma Hexachlorocyclohexane lindane and Alpha Hexachlorocyclohexane in water and a soil slurry by a pandoraea species
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Benedict C Okeke, Tariq Siddique, Marta Camps Arbestain, William T FrankenbergerAbstract:Isomers of 1,2,3,4,5,6-Hexachlorocyclohexane (HCH) were some of the most widely used pesticides. Despite reduction in their production and use, HCH isomers present a serious environmental hazard. In this study, two bacterial isolates (LIN-1 and LIN-3) that can grow on gamma-HCH as a sole source of carbon and energy were isolated from an enrichment culture. In liquid cultures of LIN-1 and LIN-3, 25.0 and 45.5% removal of gamma-HCH, respectively, were achieved in 2 weeks. LIN-3 was identified as Pandoraea sp. by 16S rRNA gene sequence analysis (99% identity). Pandoraea sp. substantially degraded both gamma- and Alpha-HCH isomers at concentrations of 10-200 mg L(-1) in liquid cultures. After 8 weeks of incubation in liquid culture, 89.9 and 93.3% of the gamma- and Alpha-HCH isomers declined, respectively, at an initial concentration of 150 mg L(-1). In soil slurry cultures of Pandoraea sp., simulating a soil slurry phase bioremediation treatment, substantial decreases in the levels of the HCH isomers were observed at concentrations of 50-200 mg L(-1). After 9 weeks, 59.6 and 53.3% biodegradations of gamma- and Alpha-HCH isomers, respectively, were achieved at 150 mg L(-1). Using two 23-mer oligonucloetide primers targeting the 330 bp region of the 16S rRNA gene of Pandoraea sp., an approximately 330 bp PCR product was successfully amplified from DNA templates prepared from bacterial colonies and soil slurry culture. This system provides a direct and rapid PCR-based molecular tool for tracking Pandoraea sp. strain LIN-3 in water and soils. These results have implied implications for the treatment of soils and water contaminated with HCH isomers.
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biodegradation of gamma Hexachlorocyclohexane lindane and Alpha Hexachlorocyclohexane in water and a soil slurry by a pandoraea species
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Benedict C Okeke, Tariq Siddique, Marta Camps Arbestain, William T FrankenbergerAbstract:Isomers of 1,2,3,4,5,6-Hexachlorocyclohexane (HCH) were some of the most widely used pesticides. Despite reduction in their production and use, HCH isomers present a serious environmental hazard. In this study, two bacterial isolates (LIN-1 and LIN-3) that can grow on γ-HCH as a sole source of carbon and energy were isolated from an enrichment culture. In liquid cultures of LIN-1 and LIN-3, 25.0 and 45.5% removal of γ-HCH, respectively, were achieved in 2 weeks. LIN-3 was identified as Pandoraea sp. by 16S rRNA gene sequence analysis (99% identity). Pandoraea sp. substantially degraded both γ- and α-HCH isomers at concentrations of 10−200 mg L-1 in liquid cultures. After 8 weeks of incubation in liquid culture, 89.9 and 93.3% of the γ- and α-HCH isomers declined, respectively, at an initial concentration of 150 mg L-1. In soil slurry cultures of Pandoraea sp., simulating a soil slurry phase bioremediation treatment, substantial decreases in the levels of the HCH isomers were observed at concentrations of ...