The Experts below are selected from a list of 237 Experts worldwide ranked by ideXlab platform
Dabing Zhang - One of the best experts on this subject based on the ideXlab platform.
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Defective Pollen Wall 3 (DPW3), a novel Alpha Integrin‐like protein, is required for pollen wall formation in rice
The New phytologist, 2019Co-Authors: Palash Chandra Mondol, Lei Duan, Jianxin Shi, Canhua Wang, Xiaofei Chen, Mingjiao Chen, Wanqi Liang, Dabing ZhangAbstract:In flowering plants, pollen wall is a specialized extracellular cell-wall matrix surrounding male gametophytes and acts as a natural protector of pollen grains against various environmental and biological stresses. The formation of pollen wall is a complex but well-regulated process, which involves the action of many different genes. However, the genetic and molecular mechanisms underlying this process remain largely unknown. In this study, we isolated and characterized a novel rice male sterile mutant, defective pollen wall3 (dpw3), which displays smaller and paler anthers with aborted pollen grains. DPW3 encodes a novel membrane-associated Alpha Integrin-like protein conserved in land plants. DPW3 is ubiquitously expressed in anther developmental stages and its protein is localized to the plasma membrane, endoplasmic reticulum (ER) and Golgi. Anthers of dpw3 plants exhibited unbalanced anther cuticular profile, abnormal Ubisch bodies, disrupted callose deposition, defective pollen wall formation such as abnormal microspore plasma membrane undulation and defective primexine formation, resulting in pollen abortion and complete male sterility. Our findings revealed a novel and vital role of Alpha Integrin-like proteins in plant male reproduction.
Palash Chandra Mondol - One of the best experts on this subject based on the ideXlab platform.
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Defective Pollen Wall 3 (DPW3), a novel Alpha Integrin‐like protein, is required for pollen wall formation in rice
The New phytologist, 2019Co-Authors: Palash Chandra Mondol, Lei Duan, Jianxin Shi, Canhua Wang, Xiaofei Chen, Mingjiao Chen, Wanqi Liang, Dabing ZhangAbstract:In flowering plants, pollen wall is a specialized extracellular cell-wall matrix surrounding male gametophytes and acts as a natural protector of pollen grains against various environmental and biological stresses. The formation of pollen wall is a complex but well-regulated process, which involves the action of many different genes. However, the genetic and molecular mechanisms underlying this process remain largely unknown. In this study, we isolated and characterized a novel rice male sterile mutant, defective pollen wall3 (dpw3), which displays smaller and paler anthers with aborted pollen grains. DPW3 encodes a novel membrane-associated Alpha Integrin-like protein conserved in land plants. DPW3 is ubiquitously expressed in anther developmental stages and its protein is localized to the plasma membrane, endoplasmic reticulum (ER) and Golgi. Anthers of dpw3 plants exhibited unbalanced anther cuticular profile, abnormal Ubisch bodies, disrupted callose deposition, defective pollen wall formation such as abnormal microspore plasma membrane undulation and defective primexine formation, resulting in pollen abortion and complete male sterility. Our findings revealed a novel and vital role of Alpha Integrin-like proteins in plant male reproduction.
Catherine C. Park - One of the best experts on this subject based on the ideXlab platform.
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Breast Cancer Cells in Three-dimensional Culture Display an Enhanced Radioresponse after Coordinate Targeting of Integrin α5β1 and Fibronectin
Cancer research, 2010Co-Authors: Jin-min Nam, Yasuhito Onodera, Mina J. Bissell, Catherine C. ParkAbstract:Tactics to selectively enhance cancer radioresponse are of great interest. Cancer cells actively elaborate and remodel their extracellular matrix (ECM) to aid in survival and progression. Previous work has shown that beta1-Integrin inhibitory antibodies can enhance the growth-inhibitory and apoptotic responses of human breast cancer cell lines to ionizing radiation, either when cells are cultured in three-dimensional laminin-rich ECM (3D lrECM) or grown as xenografts in mice. Here, we show that a specific Alpha heterodimer of beta1-Integrin preferentially mediates a prosurvival signal in human breast cancer cells that can be specifically targeted for therapy. 3D lrECM culture conditions were used to compare Alpha-Integrin heterodimer expression in malignant and nonmalignant cell lines. Under these conditions, we found that expression of Alpha5beta1-Integrin was upregulated in malignant cells compared with nonmalignant breast cells. Similarly, we found that normal and oncofetal splice variants of fibronectin, the primary ECM ligand of Alpha5beta1-Integrin, were also strikingly upregulated in malignant cell lines compared with nonmalignant acini. Cell treatment with a peptide that disrupts the interactions of Alpha5beta1-Integrin with fibronectin promoted apoptosis in malignant cells and further heightened the apoptotic effects of radiation. In support of these results, an analysis of gene expression array data from breast cancer patients revealed an association of high levels of Alpha5-Integrin expression with decreased survival. Our findings offer preclinical validation of fibronectin and Alpha5beta1-Integrin as targets for breast cancer therapy.
Jianxin Shi - One of the best experts on this subject based on the ideXlab platform.
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Defective Pollen Wall 3 (DPW3), a novel Alpha Integrin‐like protein, is required for pollen wall formation in rice
The New phytologist, 2019Co-Authors: Palash Chandra Mondol, Lei Duan, Jianxin Shi, Canhua Wang, Xiaofei Chen, Mingjiao Chen, Wanqi Liang, Dabing ZhangAbstract:In flowering plants, pollen wall is a specialized extracellular cell-wall matrix surrounding male gametophytes and acts as a natural protector of pollen grains against various environmental and biological stresses. The formation of pollen wall is a complex but well-regulated process, which involves the action of many different genes. However, the genetic and molecular mechanisms underlying this process remain largely unknown. In this study, we isolated and characterized a novel rice male sterile mutant, defective pollen wall3 (dpw3), which displays smaller and paler anthers with aborted pollen grains. DPW3 encodes a novel membrane-associated Alpha Integrin-like protein conserved in land plants. DPW3 is ubiquitously expressed in anther developmental stages and its protein is localized to the plasma membrane, endoplasmic reticulum (ER) and Golgi. Anthers of dpw3 plants exhibited unbalanced anther cuticular profile, abnormal Ubisch bodies, disrupted callose deposition, defective pollen wall formation such as abnormal microspore plasma membrane undulation and defective primexine formation, resulting in pollen abortion and complete male sterility. Our findings revealed a novel and vital role of Alpha Integrin-like proteins in plant male reproduction.
Canhua Wang - One of the best experts on this subject based on the ideXlab platform.
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Defective Pollen Wall 3 (DPW3), a novel Alpha Integrin‐like protein, is required for pollen wall formation in rice
The New phytologist, 2019Co-Authors: Palash Chandra Mondol, Lei Duan, Jianxin Shi, Canhua Wang, Xiaofei Chen, Mingjiao Chen, Wanqi Liang, Dabing ZhangAbstract:In flowering plants, pollen wall is a specialized extracellular cell-wall matrix surrounding male gametophytes and acts as a natural protector of pollen grains against various environmental and biological stresses. The formation of pollen wall is a complex but well-regulated process, which involves the action of many different genes. However, the genetic and molecular mechanisms underlying this process remain largely unknown. In this study, we isolated and characterized a novel rice male sterile mutant, defective pollen wall3 (dpw3), which displays smaller and paler anthers with aborted pollen grains. DPW3 encodes a novel membrane-associated Alpha Integrin-like protein conserved in land plants. DPW3 is ubiquitously expressed in anther developmental stages and its protein is localized to the plasma membrane, endoplasmic reticulum (ER) and Golgi. Anthers of dpw3 plants exhibited unbalanced anther cuticular profile, abnormal Ubisch bodies, disrupted callose deposition, defective pollen wall formation such as abnormal microspore plasma membrane undulation and defective primexine formation, resulting in pollen abortion and complete male sterility. Our findings revealed a novel and vital role of Alpha Integrin-like proteins in plant male reproduction.