The Experts below are selected from a list of 15 Experts worldwide ranked by ideXlab platform

Amanda Borermatsui - One of the best experts on this subject based on the ideXlab platform.

  • impact of Alpha Tocopherol Deficiency and supplementation on sacrocaudalis and gluteal muscle fiber histopathology and morphology in horses
    Journal of Veterinary Internal Medicine, 2019
    Co-Authors: Lauren Bookbinder, Carrie J Finno, Anna M Firshman, Scott A Katzman, Erin N Burns, Janel Peterson, Anna R Dahlgren, Brittni Mingwhitfield, Shelby Glessner, Amanda Borermatsui
    Abstract:

    Author(s): Bookbinder, Lauren; Finno, Carrie J; Firshman, Anna M; Katzman, Scott A; Burns, Erin; Peterson, Janel; Dahlgren, Anna; Ming-Whitfield, Brittni; Glessner, Shelby; Borer-Matsui, Amanda; Valberg, Stephanie J | Abstract: BACKGROUND:A subset of horses deficient in Alpha-Tocopherol (α-TP) develop muscle atrophy and vitamin E-responsive myopathy (VEM) characterized by mitochondrial alterations in the sacrocaudalis dorsalis medialis muscle (SC). OBJECTIVES:To quantify muscle histopathologic abnormalities in subclinical α-TP deficient horses before and after α-TP supplementation and compare with retrospective (r)VEM cases. ANIMALS:Prospective study; 16 healthy α-TP-deficient Quarter Horses. Retrospective study; 10 retrospective vitamin E-responsive myopathy (rVEM) cases . METHODS:Blood, SC, and gluteus medius (GM) biopsy specimens were obtained before (day 0) and 56 days after 5000 IU/450 kg horse/day PO water dispersible liquid α-TP (n = 8) or control (n = 8). Muscle fiber morphology and mitochondrial alterations were compared in samples from days 0 and 56 and in rVEM cases. RESULTS:Mitochondrial alterations more common than our reference range (l2.5% affected fibers) were present in 3/8 control and 4/8 treatment horses on day 0 in SC but not in GM (mean, 2.2; range, 0%-10% of fibers). Supplementation with α-TP for 56 days did not change the percentage of fibers with mitochondrial alterations or anguloid atrophy, or fiber size in GM or SC. Clinical rVEM horses had significantly more mitochondrial alterations (rVEM SC, 13% ± 7%; GM, 3% ± 2%) and anguloid atrophy compared to subclinical day 0 horses. CONCLUSIONS AND CLINICAL IMPORTANCE:Clinically normal α-TP-deficient horses can have mitochondrial alterations in the SC that are less severe than in atrophied VEM cases and do not resolve after 56 days of α-TP supplementation. Preventing α-TP Deficiency may be of long-term importance for mitochondrial viability.

Carrie J Finno - One of the best experts on this subject based on the ideXlab platform.

  • impact of Alpha Tocopherol Deficiency and supplementation on sacrocaudalis and gluteal muscle fiber histopathology and morphology in horses
    Journal of Veterinary Internal Medicine, 2019
    Co-Authors: Lauren Bookbinder, Carrie J Finno, Anna M Firshman, Scott A Katzman, Erin N Burns, Janel Peterson, Anna R Dahlgren, Brittni Mingwhitfield, Shelby Glessner, Amanda Borermatsui
    Abstract:

    Author(s): Bookbinder, Lauren; Finno, Carrie J; Firshman, Anna M; Katzman, Scott A; Burns, Erin; Peterson, Janel; Dahlgren, Anna; Ming-Whitfield, Brittni; Glessner, Shelby; Borer-Matsui, Amanda; Valberg, Stephanie J | Abstract: BACKGROUND:A subset of horses deficient in Alpha-Tocopherol (α-TP) develop muscle atrophy and vitamin E-responsive myopathy (VEM) characterized by mitochondrial alterations in the sacrocaudalis dorsalis medialis muscle (SC). OBJECTIVES:To quantify muscle histopathologic abnormalities in subclinical α-TP deficient horses before and after α-TP supplementation and compare with retrospective (r)VEM cases. ANIMALS:Prospective study; 16 healthy α-TP-deficient Quarter Horses. Retrospective study; 10 retrospective vitamin E-responsive myopathy (rVEM) cases . METHODS:Blood, SC, and gluteus medius (GM) biopsy specimens were obtained before (day 0) and 56 days after 5000 IU/450 kg horse/day PO water dispersible liquid α-TP (n = 8) or control (n = 8). Muscle fiber morphology and mitochondrial alterations were compared in samples from days 0 and 56 and in rVEM cases. RESULTS:Mitochondrial alterations more common than our reference range (l2.5% affected fibers) were present in 3/8 control and 4/8 treatment horses on day 0 in SC but not in GM (mean, 2.2; range, 0%-10% of fibers). Supplementation with α-TP for 56 days did not change the percentage of fibers with mitochondrial alterations or anguloid atrophy, or fiber size in GM or SC. Clinical rVEM horses had significantly more mitochondrial alterations (rVEM SC, 13% ± 7%; GM, 3% ± 2%) and anguloid atrophy compared to subclinical day 0 horses. CONCLUSIONS AND CLINICAL IMPORTANCE:Clinically normal α-TP-deficient horses can have mitochondrial alterations in the SC that are less severe than in atrophied VEM cases and do not resolve after 56 days of α-TP supplementation. Preventing α-TP Deficiency may be of long-term importance for mitochondrial viability.

  • an innate immune response and altered nuclear receptor activation defines the spinal cord transcriptome during Alpha Tocopherol Deficiency in ttpa null mice
    Free Radical Biology and Medicine, 2018
    Co-Authors: Carrie J Finno, Erin N Burns, Brittni Mingwhitfield, Matthew H Bordbari, Giuliana Gianino, Janel Merkel, Monica Britton, Blythe Durbinjohnson, Erica A Sloma, Marissa Z Mcmackin
    Abstract:

    Abstract Mice with Deficiency in Tocopherol (Alpha) transfer protein gene develop peripheral Tocopherol Deficiency and sensory neurodegeneration. Ttpa-/- mice maintained on diets with deficient α-Tocopherol (α-TOH) had proprioceptive deficits by six months of age, axonal degeneration and neuronal chromatolysis within the dorsal column of the spinal cord and its projections into the medulla. Transmission electron microscopy revealed degeneration of dorsal column axons. We addressed the potential pathomechanism of α-TOH deficient neurodegeneration by global transcriptome sequencing within the spinal cord and cerebellum. RNA-sequencing of the spinal cord in Ttpa-/- mice revealed upregulation of genes associated with the innate immune response, indicating a molecular signature of microglial activation as a result of Tocopherol Deficiency. For the first time, low level Ttpa expression was identified in the murine spinal cord. Further, the transcription factor liver X receptor (LXR) was strongly activated by α-TOH Deficiency, triggering dysregulation of cholesterol biosynthesis. The aberrant activation of transcription factor LXR suppressed the normal induction of the transcription factor retinoic-related orphan receptor-α (RORA), which is required for neural homeostasis. Thus we find that α-TOH Deficiency induces LXR, which may lead to a molecular signature of microglial activation and contribute to sensory neurodegeneration.

Brittni Mingwhitfield - One of the best experts on this subject based on the ideXlab platform.

  • impact of Alpha Tocopherol Deficiency and supplementation on sacrocaudalis and gluteal muscle fiber histopathology and morphology in horses
    Journal of Veterinary Internal Medicine, 2019
    Co-Authors: Lauren Bookbinder, Carrie J Finno, Anna M Firshman, Scott A Katzman, Erin N Burns, Janel Peterson, Anna R Dahlgren, Brittni Mingwhitfield, Shelby Glessner, Amanda Borermatsui
    Abstract:

    Author(s): Bookbinder, Lauren; Finno, Carrie J; Firshman, Anna M; Katzman, Scott A; Burns, Erin; Peterson, Janel; Dahlgren, Anna; Ming-Whitfield, Brittni; Glessner, Shelby; Borer-Matsui, Amanda; Valberg, Stephanie J | Abstract: BACKGROUND:A subset of horses deficient in Alpha-Tocopherol (α-TP) develop muscle atrophy and vitamin E-responsive myopathy (VEM) characterized by mitochondrial alterations in the sacrocaudalis dorsalis medialis muscle (SC). OBJECTIVES:To quantify muscle histopathologic abnormalities in subclinical α-TP deficient horses before and after α-TP supplementation and compare with retrospective (r)VEM cases. ANIMALS:Prospective study; 16 healthy α-TP-deficient Quarter Horses. Retrospective study; 10 retrospective vitamin E-responsive myopathy (rVEM) cases . METHODS:Blood, SC, and gluteus medius (GM) biopsy specimens were obtained before (day 0) and 56 days after 5000 IU/450 kg horse/day PO water dispersible liquid α-TP (n = 8) or control (n = 8). Muscle fiber morphology and mitochondrial alterations were compared in samples from days 0 and 56 and in rVEM cases. RESULTS:Mitochondrial alterations more common than our reference range (l2.5% affected fibers) were present in 3/8 control and 4/8 treatment horses on day 0 in SC but not in GM (mean, 2.2; range, 0%-10% of fibers). Supplementation with α-TP for 56 days did not change the percentage of fibers with mitochondrial alterations or anguloid atrophy, or fiber size in GM or SC. Clinical rVEM horses had significantly more mitochondrial alterations (rVEM SC, 13% ± 7%; GM, 3% ± 2%) and anguloid atrophy compared to subclinical day 0 horses. CONCLUSIONS AND CLINICAL IMPORTANCE:Clinically normal α-TP-deficient horses can have mitochondrial alterations in the SC that are less severe than in atrophied VEM cases and do not resolve after 56 days of α-TP supplementation. Preventing α-TP Deficiency may be of long-term importance for mitochondrial viability.

  • an innate immune response and altered nuclear receptor activation defines the spinal cord transcriptome during Alpha Tocopherol Deficiency in ttpa null mice
    Free Radical Biology and Medicine, 2018
    Co-Authors: Carrie J Finno, Erin N Burns, Brittni Mingwhitfield, Matthew H Bordbari, Giuliana Gianino, Janel Merkel, Monica Britton, Blythe Durbinjohnson, Erica A Sloma, Marissa Z Mcmackin
    Abstract:

    Abstract Mice with Deficiency in Tocopherol (Alpha) transfer protein gene develop peripheral Tocopherol Deficiency and sensory neurodegeneration. Ttpa-/- mice maintained on diets with deficient α-Tocopherol (α-TOH) had proprioceptive deficits by six months of age, axonal degeneration and neuronal chromatolysis within the dorsal column of the spinal cord and its projections into the medulla. Transmission electron microscopy revealed degeneration of dorsal column axons. We addressed the potential pathomechanism of α-TOH deficient neurodegeneration by global transcriptome sequencing within the spinal cord and cerebellum. RNA-sequencing of the spinal cord in Ttpa-/- mice revealed upregulation of genes associated with the innate immune response, indicating a molecular signature of microglial activation as a result of Tocopherol Deficiency. For the first time, low level Ttpa expression was identified in the murine spinal cord. Further, the transcription factor liver X receptor (LXR) was strongly activated by α-TOH Deficiency, triggering dysregulation of cholesterol biosynthesis. The aberrant activation of transcription factor LXR suppressed the normal induction of the transcription factor retinoic-related orphan receptor-α (RORA), which is required for neural homeostasis. Thus we find that α-TOH Deficiency induces LXR, which may lead to a molecular signature of microglial activation and contribute to sensory neurodegeneration.

Erin N Burns - One of the best experts on this subject based on the ideXlab platform.

  • impact of Alpha Tocopherol Deficiency and supplementation on sacrocaudalis and gluteal muscle fiber histopathology and morphology in horses
    Journal of Veterinary Internal Medicine, 2019
    Co-Authors: Lauren Bookbinder, Carrie J Finno, Anna M Firshman, Scott A Katzman, Erin N Burns, Janel Peterson, Anna R Dahlgren, Brittni Mingwhitfield, Shelby Glessner, Amanda Borermatsui
    Abstract:

    Author(s): Bookbinder, Lauren; Finno, Carrie J; Firshman, Anna M; Katzman, Scott A; Burns, Erin; Peterson, Janel; Dahlgren, Anna; Ming-Whitfield, Brittni; Glessner, Shelby; Borer-Matsui, Amanda; Valberg, Stephanie J | Abstract: BACKGROUND:A subset of horses deficient in Alpha-Tocopherol (α-TP) develop muscle atrophy and vitamin E-responsive myopathy (VEM) characterized by mitochondrial alterations in the sacrocaudalis dorsalis medialis muscle (SC). OBJECTIVES:To quantify muscle histopathologic abnormalities in subclinical α-TP deficient horses before and after α-TP supplementation and compare with retrospective (r)VEM cases. ANIMALS:Prospective study; 16 healthy α-TP-deficient Quarter Horses. Retrospective study; 10 retrospective vitamin E-responsive myopathy (rVEM) cases . METHODS:Blood, SC, and gluteus medius (GM) biopsy specimens were obtained before (day 0) and 56 days after 5000 IU/450 kg horse/day PO water dispersible liquid α-TP (n = 8) or control (n = 8). Muscle fiber morphology and mitochondrial alterations were compared in samples from days 0 and 56 and in rVEM cases. RESULTS:Mitochondrial alterations more common than our reference range (l2.5% affected fibers) were present in 3/8 control and 4/8 treatment horses on day 0 in SC but not in GM (mean, 2.2; range, 0%-10% of fibers). Supplementation with α-TP for 56 days did not change the percentage of fibers with mitochondrial alterations or anguloid atrophy, or fiber size in GM or SC. Clinical rVEM horses had significantly more mitochondrial alterations (rVEM SC, 13% ± 7%; GM, 3% ± 2%) and anguloid atrophy compared to subclinical day 0 horses. CONCLUSIONS AND CLINICAL IMPORTANCE:Clinically normal α-TP-deficient horses can have mitochondrial alterations in the SC that are less severe than in atrophied VEM cases and do not resolve after 56 days of α-TP supplementation. Preventing α-TP Deficiency may be of long-term importance for mitochondrial viability.

  • an innate immune response and altered nuclear receptor activation defines the spinal cord transcriptome during Alpha Tocopherol Deficiency in ttpa null mice
    Free Radical Biology and Medicine, 2018
    Co-Authors: Carrie J Finno, Erin N Burns, Brittni Mingwhitfield, Matthew H Bordbari, Giuliana Gianino, Janel Merkel, Monica Britton, Blythe Durbinjohnson, Erica A Sloma, Marissa Z Mcmackin
    Abstract:

    Abstract Mice with Deficiency in Tocopherol (Alpha) transfer protein gene develop peripheral Tocopherol Deficiency and sensory neurodegeneration. Ttpa-/- mice maintained on diets with deficient α-Tocopherol (α-TOH) had proprioceptive deficits by six months of age, axonal degeneration and neuronal chromatolysis within the dorsal column of the spinal cord and its projections into the medulla. Transmission electron microscopy revealed degeneration of dorsal column axons. We addressed the potential pathomechanism of α-TOH deficient neurodegeneration by global transcriptome sequencing within the spinal cord and cerebellum. RNA-sequencing of the spinal cord in Ttpa-/- mice revealed upregulation of genes associated with the innate immune response, indicating a molecular signature of microglial activation as a result of Tocopherol Deficiency. For the first time, low level Ttpa expression was identified in the murine spinal cord. Further, the transcription factor liver X receptor (LXR) was strongly activated by α-TOH Deficiency, triggering dysregulation of cholesterol biosynthesis. The aberrant activation of transcription factor LXR suppressed the normal induction of the transcription factor retinoic-related orphan receptor-α (RORA), which is required for neural homeostasis. Thus we find that α-TOH Deficiency induces LXR, which may lead to a molecular signature of microglial activation and contribute to sensory neurodegeneration.

Lauren Bookbinder - One of the best experts on this subject based on the ideXlab platform.

  • impact of Alpha Tocopherol Deficiency and supplementation on sacrocaudalis and gluteal muscle fiber histopathology and morphology in horses
    Journal of Veterinary Internal Medicine, 2019
    Co-Authors: Lauren Bookbinder, Carrie J Finno, Anna M Firshman, Scott A Katzman, Erin N Burns, Janel Peterson, Anna R Dahlgren, Brittni Mingwhitfield, Shelby Glessner, Amanda Borermatsui
    Abstract:

    Author(s): Bookbinder, Lauren; Finno, Carrie J; Firshman, Anna M; Katzman, Scott A; Burns, Erin; Peterson, Janel; Dahlgren, Anna; Ming-Whitfield, Brittni; Glessner, Shelby; Borer-Matsui, Amanda; Valberg, Stephanie J | Abstract: BACKGROUND:A subset of horses deficient in Alpha-Tocopherol (α-TP) develop muscle atrophy and vitamin E-responsive myopathy (VEM) characterized by mitochondrial alterations in the sacrocaudalis dorsalis medialis muscle (SC). OBJECTIVES:To quantify muscle histopathologic abnormalities in subclinical α-TP deficient horses before and after α-TP supplementation and compare with retrospective (r)VEM cases. ANIMALS:Prospective study; 16 healthy α-TP-deficient Quarter Horses. Retrospective study; 10 retrospective vitamin E-responsive myopathy (rVEM) cases . METHODS:Blood, SC, and gluteus medius (GM) biopsy specimens were obtained before (day 0) and 56 days after 5000 IU/450 kg horse/day PO water dispersible liquid α-TP (n = 8) or control (n = 8). Muscle fiber morphology and mitochondrial alterations were compared in samples from days 0 and 56 and in rVEM cases. RESULTS:Mitochondrial alterations more common than our reference range (l2.5% affected fibers) were present in 3/8 control and 4/8 treatment horses on day 0 in SC but not in GM (mean, 2.2; range, 0%-10% of fibers). Supplementation with α-TP for 56 days did not change the percentage of fibers with mitochondrial alterations or anguloid atrophy, or fiber size in GM or SC. Clinical rVEM horses had significantly more mitochondrial alterations (rVEM SC, 13% ± 7%; GM, 3% ± 2%) and anguloid atrophy compared to subclinical day 0 horses. CONCLUSIONS AND CLINICAL IMPORTANCE:Clinically normal α-TP-deficient horses can have mitochondrial alterations in the SC that are less severe than in atrophied VEM cases and do not resolve after 56 days of α-TP supplementation. Preventing α-TP Deficiency may be of long-term importance for mitochondrial viability.