The Experts below are selected from a list of 160782 Experts worldwide ranked by ideXlab platform
Paul J Orchard - One of the best experts on this subject based on the ideXlab platform.
-
coexpression of the uracil phosphoribosyltransferase Gene with a chimeric human nerve growth factor receptor cytosine deaminase fusion Gene using a single retroviral vector augments cytotoxicity of transduced human t cells exposed to 5 fluorocytosine
Human Gene Therapy, 2006Co-Authors: Tracey A Obrien, Dong T Tuong, Lisa Basso, Scott R. Mcivor, Paul J OrchardAbstract:Donor T lymphocytes Genetically engineered to express a "suicide Gene" to facilitate negative selection represent a promising strategy for the management of graft-versus-host disease occurring after alloGeneic hematopoietic cell transplantation (HCT). For this purpose, the herpes simplex virus thymidine kinase (HSV-tk) Gene, although well studied, has limitations. Cytosine deaminase (CD), an Alternative Gene for negative selection, converts 5-fluorocytosine (5-FC) to the toxic metabolite 5-fluorouracil (5-FU). Sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase (UPRT), which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate. By using a chimeric Gene (NG/CD) expressing the truncated human nerve growth factor receptor (NGFR) for positive selection fused to the Saccharomyces cerevisiae CD Gene, we investigated strategies to achieve optimal T cell eradication by CD and UPRT expression, utilizing a single retroviral vector. Three vector strate...
-
Coexpression of the uracil phosphoribosyltransferase Gene with a chimeric human nerve growth factor receptor/cytosine deaminase fusion Gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells exposed to 5-fluorocytosi
Human Gene Therapy, 2006Co-Authors: Tracey A. O'brien, Dong T Tuong, Lisa Basso, R. Scott Mcivor, Paul J OrchardAbstract:Donor T lymphocytes Genetically engineered to express a "suicide Gene" to facilitate negative selection represent a promising strategy for the management of graft-versus-host disease occurring after alloGeneic hematopoietic cell transplantation (HCT). For this purpose, the herpes simplex virus thymidine kinase (HSV-tk) Gene, although well studied, has limitations. Cytosine deaminase (CD), an Alternative Gene for negative selection, converts 5-fluorocytosine (5-FC) to the toxic metabolite 5-fluorouracil (5-FU). Sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase (UPRT), which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate. By using a chimeric Gene (NG/CD) expressing the truncated human nerve growth factor receptor (NGFR) for positive selection fused to the Saccharomyces cerevisiae CD Gene, we investigated strategies to achieve optimal T cell eradication by CD and UPRT expression, utilizing a single retroviral vector. Three vector strate...
Lisa Basso - One of the best experts on this subject based on the ideXlab platform.
-
coexpression of the uracil phosphoribosyltransferase Gene with a chimeric human nerve growth factor receptor cytosine deaminase fusion Gene using a single retroviral vector augments cytotoxicity of transduced human t cells exposed to 5 fluorocytosine
Human Gene Therapy, 2006Co-Authors: Tracey A Obrien, Dong T Tuong, Lisa Basso, Scott R. Mcivor, Paul J OrchardAbstract:Donor T lymphocytes Genetically engineered to express a "suicide Gene" to facilitate negative selection represent a promising strategy for the management of graft-versus-host disease occurring after alloGeneic hematopoietic cell transplantation (HCT). For this purpose, the herpes simplex virus thymidine kinase (HSV-tk) Gene, although well studied, has limitations. Cytosine deaminase (CD), an Alternative Gene for negative selection, converts 5-fluorocytosine (5-FC) to the toxic metabolite 5-fluorouracil (5-FU). Sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase (UPRT), which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate. By using a chimeric Gene (NG/CD) expressing the truncated human nerve growth factor receptor (NGFR) for positive selection fused to the Saccharomyces cerevisiae CD Gene, we investigated strategies to achieve optimal T cell eradication by CD and UPRT expression, utilizing a single retroviral vector. Three vector strate...
-
Coexpression of the uracil phosphoribosyltransferase Gene with a chimeric human nerve growth factor receptor/cytosine deaminase fusion Gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells exposed to 5-fluorocytosi
Human Gene Therapy, 2006Co-Authors: Tracey A. O'brien, Dong T Tuong, Lisa Basso, R. Scott Mcivor, Paul J OrchardAbstract:Donor T lymphocytes Genetically engineered to express a "suicide Gene" to facilitate negative selection represent a promising strategy for the management of graft-versus-host disease occurring after alloGeneic hematopoietic cell transplantation (HCT). For this purpose, the herpes simplex virus thymidine kinase (HSV-tk) Gene, although well studied, has limitations. Cytosine deaminase (CD), an Alternative Gene for negative selection, converts 5-fluorocytosine (5-FC) to the toxic metabolite 5-fluorouracil (5-FU). Sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase (UPRT), which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate. By using a chimeric Gene (NG/CD) expressing the truncated human nerve growth factor receptor (NGFR) for positive selection fused to the Saccharomyces cerevisiae CD Gene, we investigated strategies to achieve optimal T cell eradication by CD and UPRT expression, utilizing a single retroviral vector. Three vector strate...
Dong T Tuong - One of the best experts on this subject based on the ideXlab platform.
-
coexpression of the uracil phosphoribosyltransferase Gene with a chimeric human nerve growth factor receptor cytosine deaminase fusion Gene using a single retroviral vector augments cytotoxicity of transduced human t cells exposed to 5 fluorocytosine
Human Gene Therapy, 2006Co-Authors: Tracey A Obrien, Dong T Tuong, Lisa Basso, Scott R. Mcivor, Paul J OrchardAbstract:Donor T lymphocytes Genetically engineered to express a "suicide Gene" to facilitate negative selection represent a promising strategy for the management of graft-versus-host disease occurring after alloGeneic hematopoietic cell transplantation (HCT). For this purpose, the herpes simplex virus thymidine kinase (HSV-tk) Gene, although well studied, has limitations. Cytosine deaminase (CD), an Alternative Gene for negative selection, converts 5-fluorocytosine (5-FC) to the toxic metabolite 5-fluorouracil (5-FU). Sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase (UPRT), which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate. By using a chimeric Gene (NG/CD) expressing the truncated human nerve growth factor receptor (NGFR) for positive selection fused to the Saccharomyces cerevisiae CD Gene, we investigated strategies to achieve optimal T cell eradication by CD and UPRT expression, utilizing a single retroviral vector. Three vector strate...
-
Coexpression of the uracil phosphoribosyltransferase Gene with a chimeric human nerve growth factor receptor/cytosine deaminase fusion Gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells exposed to 5-fluorocytosi
Human Gene Therapy, 2006Co-Authors: Tracey A. O'brien, Dong T Tuong, Lisa Basso, R. Scott Mcivor, Paul J OrchardAbstract:Donor T lymphocytes Genetically engineered to express a "suicide Gene" to facilitate negative selection represent a promising strategy for the management of graft-versus-host disease occurring after alloGeneic hematopoietic cell transplantation (HCT). For this purpose, the herpes simplex virus thymidine kinase (HSV-tk) Gene, although well studied, has limitations. Cytosine deaminase (CD), an Alternative Gene for negative selection, converts 5-fluorocytosine (5-FC) to the toxic metabolite 5-fluorouracil (5-FU). Sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase (UPRT), which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate. By using a chimeric Gene (NG/CD) expressing the truncated human nerve growth factor receptor (NGFR) for positive selection fused to the Saccharomyces cerevisiae CD Gene, we investigated strategies to achieve optimal T cell eradication by CD and UPRT expression, utilizing a single retroviral vector. Three vector strate...
Tracey A. O'brien - One of the best experts on this subject based on the ideXlab platform.
-
Coexpression of the uracil phosphoribosyltransferase Gene with a chimeric human nerve growth factor receptor/cytosine deaminase fusion Gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells exposed to 5-fluorocytosi
Human Gene Therapy, 2006Co-Authors: Tracey A. O'brien, Dong T Tuong, Lisa Basso, R. Scott Mcivor, Paul J OrchardAbstract:Donor T lymphocytes Genetically engineered to express a "suicide Gene" to facilitate negative selection represent a promising strategy for the management of graft-versus-host disease occurring after alloGeneic hematopoietic cell transplantation (HCT). For this purpose, the herpes simplex virus thymidine kinase (HSV-tk) Gene, although well studied, has limitations. Cytosine deaminase (CD), an Alternative Gene for negative selection, converts 5-fluorocytosine (5-FC) to the toxic metabolite 5-fluorouracil (5-FU). Sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase (UPRT), which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate. By using a chimeric Gene (NG/CD) expressing the truncated human nerve growth factor receptor (NGFR) for positive selection fused to the Saccharomyces cerevisiae CD Gene, we investigated strategies to achieve optimal T cell eradication by CD and UPRT expression, utilizing a single retroviral vector. Three vector strate...
Tracey A Obrien - One of the best experts on this subject based on the ideXlab platform.
-
coexpression of the uracil phosphoribosyltransferase Gene with a chimeric human nerve growth factor receptor cytosine deaminase fusion Gene using a single retroviral vector augments cytotoxicity of transduced human t cells exposed to 5 fluorocytosine
Human Gene Therapy, 2006Co-Authors: Tracey A Obrien, Dong T Tuong, Lisa Basso, Scott R. Mcivor, Paul J OrchardAbstract:Donor T lymphocytes Genetically engineered to express a "suicide Gene" to facilitate negative selection represent a promising strategy for the management of graft-versus-host disease occurring after alloGeneic hematopoietic cell transplantation (HCT). For this purpose, the herpes simplex virus thymidine kinase (HSV-tk) Gene, although well studied, has limitations. Cytosine deaminase (CD), an Alternative Gene for negative selection, converts 5-fluorocytosine (5-FC) to the toxic metabolite 5-fluorouracil (5-FU). Sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase (UPRT), which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate. By using a chimeric Gene (NG/CD) expressing the truncated human nerve growth factor receptor (NGFR) for positive selection fused to the Saccharomyces cerevisiae CD Gene, we investigated strategies to achieve optimal T cell eradication by CD and UPRT expression, utilizing a single retroviral vector. Three vector strate...
