The Experts below are selected from a list of 48351 Experts worldwide ranked by ideXlab platform

Mercedes M Marotovaler - One of the best experts on this subject based on the ideXlab platform.

  • mineral carbonation from metal wastes effect of solid to liquid ratio on the efficiency and characterization of carbonated products
    Applied Energy, 2014
    Co-Authors: Aimaro Sanna, Mercedes M Marotovaler
    Abstract:

    Mineral carbonation technologies aim at permanently storing CO2 into materials rich in metal oxides. A multi-step mineralization Process employing Ca-rich waste streams to precipitate calcium carbonate is investigated in this paper. Ground granulated blast furnace slag (GGBS), phosphorus slag (PS) and steel slag (SS) were employed as feeding materials for the Process. Solid to liquid ratio (S/L) is an important factor which affects mineral carbonation and this study examines its effect on the carbonation efficiencies. The main phases present in the carbonated residues were identified using XRF, XRD and SEM–EDS analytical techniques. For the three materials investigated, the carbonation efficiency increased when the S/L ratio decreased (from 50g/L to 25g/L and then 15g/L) because of the dilution effect. In a previous study, where an Analog Process was employed, efficiency using serpentine was found lower than that calculated here for GGBS and SS, and slightly above PS. This confirms that, in general, waste materials require less energy-intensive carbonation conditions, in comparison to mineral rocks. Finally, the structure of the carbonated particles is also discussed.

  • mineral carbonation from metal wastes effect of solid to liquid ratio on the efficiency and characterization of carbonated products
    Applied Energy, 2014
    Co-Authors: Marco Dri, Aimaro Sanna, Mercedes M Marotovaler
    Abstract:

    Abstract Mineral carbonation technologies aim at permanently storing CO 2 into materials rich in metal oxides. A multi-step mineralization Process employing Ca-rich waste streams to precipitate calcium carbonate is investigated in this paper. Ground granulated blast furnace slag (GGBS), phosphorus slag (PS) and steel slag (SS) were employed as feeding materials for the Process. Solid to liquid ratio (S/L) is an important factor which affects mineral carbonation and this study examines its effect on the carbonation efficiencies. The main phases present in the carbonated residues were identified using XRF, XRD and SEM–EDS analytical techniques. For the three materials investigated, the carbonation efficiency increased when the S/L ratio decreased (from 50 g/L to 25 g/L and then 15 g/L) because of the dilution effect. In a previous study, where an Analog Process was employed, efficiency using serpentine was found lower than that calculated here for GGBS and SS, and slightly above PS. This confirms that, in general, waste materials require less energy-intensive carbonation conditions, in comparison to mineral rocks. Finally, the structure of the carbonated particles is also discussed.

Revaz Chachanidze - One of the best experts on this subject based on the ideXlab platform.

  • glutaraldehyde a subtle tool in the investigation of healthy and pathologic red blood cells
    Frontiers in Physiology, 2019
    Co-Authors: Asena Abay, Greta Simionato, Revaz Chachanidze, Anna Bogdanova, Laura Hertz, Paola Bianchi, Emile Van Den Akker, Marieke Von Lindern, Marc Leonetti
    Abstract:

    Glutaraldehyde is a well-known substance used in biomedical research to fix cells. Since hemolytic anemias are often associated with red blood cell shape changes deviating from the biconcave disk shape, conservation of these shapes for imaging in general and 3D-imaging in particular, like confocal microscopy, scanning electron microscopy or scanning probe microscopy is a common desire. Along with the fixation comes an increase in the stiffness of the cells. In the context of red blood cells this increased rigidity is often used to mimic malaria infected red blood cells because they are also stiffer than healthy red blood cells. However, the use of glutaraldehyde is associated with numerous pitfalls: (i) while the increase in rigidity by an application of increasing concentrations of glutaraldehyde is an Analog Process, the fixation is a rather digital event (all or none); (ii) addition of glutaraldehyde massively changes osmolality in a concentration dependent manner and hence cell shapes can be distorted; (iii) glutaraldehyde batches differ in their properties especially in the ratio of monomers and polymers; (iv) handling pitfalls, like inducing shear artifacts of red blood cell shapes or cell density changes that needs to be considered, e.g., when working with cells in flow; (v) staining glutaraldehyde treated red blood cells need different approaches compared to living cells, for instance, because glutaraldehyde itself induces a strong fluorescence. Within this paper we provide documentation about the subtle use of glutaraldehyde on healthy and pathologic red blood cells and how to deal with or circumvent pitfalls.

  • Glutaraldehyde – A Subtle Tool in the Investigation of Healthy and Pathologic Red Blood Cells
    Frontiers Media S.A., 2019
    Co-Authors: Asena Abay, Greta Simionato, Revaz Chachanidze, Anna Bogdanova, Laura Hertz, Paola Bianchi
    Abstract:

    Glutaraldehyde is a well-known substance used in biomedical research to fix cells. Since hemolytic anemias are often associated with red blood cell shape changes deviating from the biconcave disk shape, conservation of these shapes for imaging in general and 3D-imaging in particular, like confocal microscopy, scanning electron microscopy or scanning probe microscopy is a common desire. Along with the fixation comes an increase in the stiffness of the cells. In the context of red blood cells this increased rigidity is often used to mimic malaria infected red blood cells because they are also stiffer than healthy red blood cells. However, the use of glutaraldehyde is associated with numerous pitfalls: (i) while the increase in rigidity by an application of increasing concentrations of glutaraldehyde is an Analog Process, the fixation is a rather digital event (all or none); (ii) addition of glutaraldehyde massively changes osmolality in a concentration dependent manner and hence cell shapes can be distorted; (iii) glutaraldehyde batches differ in their properties especially in the ratio of monomers and polymers; (iv) handling pitfalls, like inducing shear artifacts of red blood cell shapes or cell density changes that needs to be considered, e.g., when working with cells in flow; (v) staining glutaraldehyde treated red blood cells need different approaches compared to living cells, for instance, because glutaraldehyde itself induces a strong fluorescence. Within this paper we provide documentation about the subtle use of glutaraldehyde on healthy and pathologic red blood cells and how to deal with or circumvent pitfalls

Marc Leonetti - One of the best experts on this subject based on the ideXlab platform.

  • glutaraldehyde a subtle tool in the investigation of healthy and pathologic red blood cells
    Frontiers in Physiology, 2019
    Co-Authors: Asena Abay, Greta Simionato, Revaz Chachanidze, Anna Bogdanova, Laura Hertz, Paola Bianchi, Emile Van Den Akker, Marieke Von Lindern, Marc Leonetti
    Abstract:

    Glutaraldehyde is a well-known substance used in biomedical research to fix cells. Since hemolytic anemias are often associated with red blood cell shape changes deviating from the biconcave disk shape, conservation of these shapes for imaging in general and 3D-imaging in particular, like confocal microscopy, scanning electron microscopy or scanning probe microscopy is a common desire. Along with the fixation comes an increase in the stiffness of the cells. In the context of red blood cells this increased rigidity is often used to mimic malaria infected red blood cells because they are also stiffer than healthy red blood cells. However, the use of glutaraldehyde is associated with numerous pitfalls: (i) while the increase in rigidity by an application of increasing concentrations of glutaraldehyde is an Analog Process, the fixation is a rather digital event (all or none); (ii) addition of glutaraldehyde massively changes osmolality in a concentration dependent manner and hence cell shapes can be distorted; (iii) glutaraldehyde batches differ in their properties especially in the ratio of monomers and polymers; (iv) handling pitfalls, like inducing shear artifacts of red blood cell shapes or cell density changes that needs to be considered, e.g., when working with cells in flow; (v) staining glutaraldehyde treated red blood cells need different approaches compared to living cells, for instance, because glutaraldehyde itself induces a strong fluorescence. Within this paper we provide documentation about the subtle use of glutaraldehyde on healthy and pathologic red blood cells and how to deal with or circumvent pitfalls.

Asena Abay - One of the best experts on this subject based on the ideXlab platform.

  • glutaraldehyde a subtle tool in the investigation of healthy and pathologic red blood cells
    Frontiers in Physiology, 2019
    Co-Authors: Asena Abay, Greta Simionato, Revaz Chachanidze, Anna Bogdanova, Laura Hertz, Paola Bianchi, Emile Van Den Akker, Marieke Von Lindern, Marc Leonetti
    Abstract:

    Glutaraldehyde is a well-known substance used in biomedical research to fix cells. Since hemolytic anemias are often associated with red blood cell shape changes deviating from the biconcave disk shape, conservation of these shapes for imaging in general and 3D-imaging in particular, like confocal microscopy, scanning electron microscopy or scanning probe microscopy is a common desire. Along with the fixation comes an increase in the stiffness of the cells. In the context of red blood cells this increased rigidity is often used to mimic malaria infected red blood cells because they are also stiffer than healthy red blood cells. However, the use of glutaraldehyde is associated with numerous pitfalls: (i) while the increase in rigidity by an application of increasing concentrations of glutaraldehyde is an Analog Process, the fixation is a rather digital event (all or none); (ii) addition of glutaraldehyde massively changes osmolality in a concentration dependent manner and hence cell shapes can be distorted; (iii) glutaraldehyde batches differ in their properties especially in the ratio of monomers and polymers; (iv) handling pitfalls, like inducing shear artifacts of red blood cell shapes or cell density changes that needs to be considered, e.g., when working with cells in flow; (v) staining glutaraldehyde treated red blood cells need different approaches compared to living cells, for instance, because glutaraldehyde itself induces a strong fluorescence. Within this paper we provide documentation about the subtle use of glutaraldehyde on healthy and pathologic red blood cells and how to deal with or circumvent pitfalls.

  • Glutaraldehyde – A Subtle Tool in the Investigation of Healthy and Pathologic Red Blood Cells
    Frontiers Media S.A., 2019
    Co-Authors: Asena Abay, Greta Simionato, Revaz Chachanidze, Anna Bogdanova, Laura Hertz, Paola Bianchi
    Abstract:

    Glutaraldehyde is a well-known substance used in biomedical research to fix cells. Since hemolytic anemias are often associated with red blood cell shape changes deviating from the biconcave disk shape, conservation of these shapes for imaging in general and 3D-imaging in particular, like confocal microscopy, scanning electron microscopy or scanning probe microscopy is a common desire. Along with the fixation comes an increase in the stiffness of the cells. In the context of red blood cells this increased rigidity is often used to mimic malaria infected red blood cells because they are also stiffer than healthy red blood cells. However, the use of glutaraldehyde is associated with numerous pitfalls: (i) while the increase in rigidity by an application of increasing concentrations of glutaraldehyde is an Analog Process, the fixation is a rather digital event (all or none); (ii) addition of glutaraldehyde massively changes osmolality in a concentration dependent manner and hence cell shapes can be distorted; (iii) glutaraldehyde batches differ in their properties especially in the ratio of monomers and polymers; (iv) handling pitfalls, like inducing shear artifacts of red blood cell shapes or cell density changes that needs to be considered, e.g., when working with cells in flow; (v) staining glutaraldehyde treated red blood cells need different approaches compared to living cells, for instance, because glutaraldehyde itself induces a strong fluorescence. Within this paper we provide documentation about the subtle use of glutaraldehyde on healthy and pathologic red blood cells and how to deal with or circumvent pitfalls

Aimaro Sanna - One of the best experts on this subject based on the ideXlab platform.

  • mineral carbonation from metal wastes effect of solid to liquid ratio on the efficiency and characterization of carbonated products
    Applied Energy, 2014
    Co-Authors: Aimaro Sanna, Mercedes M Marotovaler
    Abstract:

    Mineral carbonation technologies aim at permanently storing CO2 into materials rich in metal oxides. A multi-step mineralization Process employing Ca-rich waste streams to precipitate calcium carbonate is investigated in this paper. Ground granulated blast furnace slag (GGBS), phosphorus slag (PS) and steel slag (SS) were employed as feeding materials for the Process. Solid to liquid ratio (S/L) is an important factor which affects mineral carbonation and this study examines its effect on the carbonation efficiencies. The main phases present in the carbonated residues were identified using XRF, XRD and SEM–EDS analytical techniques. For the three materials investigated, the carbonation efficiency increased when the S/L ratio decreased (from 50g/L to 25g/L and then 15g/L) because of the dilution effect. In a previous study, where an Analog Process was employed, efficiency using serpentine was found lower than that calculated here for GGBS and SS, and slightly above PS. This confirms that, in general, waste materials require less energy-intensive carbonation conditions, in comparison to mineral rocks. Finally, the structure of the carbonated particles is also discussed.

  • mineral carbonation from metal wastes effect of solid to liquid ratio on the efficiency and characterization of carbonated products
    Applied Energy, 2014
    Co-Authors: Marco Dri, Aimaro Sanna, Mercedes M Marotovaler
    Abstract:

    Abstract Mineral carbonation technologies aim at permanently storing CO 2 into materials rich in metal oxides. A multi-step mineralization Process employing Ca-rich waste streams to precipitate calcium carbonate is investigated in this paper. Ground granulated blast furnace slag (GGBS), phosphorus slag (PS) and steel slag (SS) were employed as feeding materials for the Process. Solid to liquid ratio (S/L) is an important factor which affects mineral carbonation and this study examines its effect on the carbonation efficiencies. The main phases present in the carbonated residues were identified using XRF, XRD and SEM–EDS analytical techniques. For the three materials investigated, the carbonation efficiency increased when the S/L ratio decreased (from 50 g/L to 25 g/L and then 15 g/L) because of the dilution effect. In a previous study, where an Analog Process was employed, efficiency using serpentine was found lower than that calculated here for GGBS and SS, and slightly above PS. This confirms that, in general, waste materials require less energy-intensive carbonation conditions, in comparison to mineral rocks. Finally, the structure of the carbonated particles is also discussed.