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Jen Hwey Chiu - One of the best experts on this subject based on the ideXlab platform.

  • chrysin suppresses il 6 induced Angiogenesis via down regulation of jak1 stat3 and vegf an in vitro and in ovo approach
    Journal of Agricultural and Food Chemistry, 2010
    Co-Authors: Kou-gi Shyu, Bao Wei Wang, Hang Chang, Yenhsu Chen, Jen Hwey Chiu
    Abstract:

    Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced Angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane Assay, an in ovo Angiogenesis Assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiAngiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced Angiogenesis through modulation of the sIL-6R/gp130/JAK...

  • chrysin suppresses il 6 induced Angiogenesis via down regulation of jak1 stat3 and vegf an in vitro and in ovo approach
    Journal of Agricultural and Food Chemistry, 2010
    Co-Authors: Chiu Mei Lin, Bao Wei Wang, Hang Chang, Yenhsu Chen, Kou-gi Shyu, Jen Hwey Chiu
    Abstract:

    Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced Angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane Assay, an in ovo Angiogenesis Assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiAngiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced Angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signaling pathway. Chrysin may provide new therapeutic potential for IL-6-induced pathological Angiogenesis.

Marco Presta - One of the best experts on this subject based on the ideXlab platform.

  • fibroblast growth factor 2 induced Angiogenesis in zebrafish the zebrafish yolk membrane zfym Angiogenesis Assay
    Journal of Cellular and Molecular Medicine, 2009
    Co-Authors: Stefania Nicoli, Giulia De Sena, Marco Presta
    Abstract:

    Angiogenesis plays a key role in tumour growth and metastasis. The teleost zebrafish (Danio rerio) represents a promising alternative model in cancer research. Here, we describe a zebrafish yolk membrane (ZFYM) Angiogenesis Assays based on the injection of 1–30 ng of human recombinant FGF2 (rFGF2) in the perivitelline space of zebrafish embryos in the proximity of developing subintestinal vein vessels (SIVs) at 48 hrs after fertilization. The rFGF2 induces a rapid and dose-dependent angiogenic response from the SIV basket, characterized by the ectopic growth of newly formed, alkaline phosphatase-positive blood vessels. These vessels are formed by proliferating cells that incorporate bromodeoxyuridine and express the endothelial cell markers vegfr2/kdr and fli1. Microangiography shows that rFGF2-induced vessels are patent and connected to the systemic circulation of the embryo. In keeping with these observations, fli1:EGFP+ cells isolated from transgenic tg(fli1:EGFP)y1 zebrafish embryos express the tyrosine kinase (TK) FGF receptor-1 (FGFR1) and activate extracellular signal-regulated kinase signalling when stimulated in vitro by rFGF2. The low molecular weight TK-FGFR1 inhibitor SU5402 and the high molecular weight FGF2 antagonist long-pentraxin 3 inhibit the angiogenic activity of rFGF2 when added to fish water or when co-injected with the growth factor, respectively. Moreover, similar to rFGF2, injection of the zebrafish form of vascular endothelial growth factor-A (VEGF-A) induces a significant angiogenic response in the ZFYM Assay that is suppressed by the VEGF receptor-2/KDR TK inhibitor SU5416. The ZFYM Assay represents a novel tool for testing the activity of low and high molecular weight inhibitors targeting a defined angiogenic growth factor in zebrafish. The Assay may offer significant advantages when compared to other animal models.

  • the zebrafish tumor xenograft Angiogenesis Assay
    Nature Protocols, 2007
    Co-Authors: Stefania Nicoli, Marco Presta
    Abstract:

    Zebrafish (Danio rerio) represents a powerful model system in cancer research. Recent observations have shown the possibility to exploit zebrafish to investigate tumor Angiogenesis, a pivotal step in cancer progression and target for anti-tumor therapies. Novel genetic tools and high resolutionin vivo imaging techniques are also becoming available in zebrafish. It is anticipated that zebrafish will represent an important tool for chemical discovery and gene targeting in tumor Angiogenesis. Here we describe a method to study tumor Angiogenesis in zebrafish (Danio rerio) based on the injection of proangiogenic mammalian tumor cells into the perivitelline space of zebrafish embryos at 48 h post-fertilization. Within 24–48 h, proangiogenic tumor grafts induce a neovascular response originating from the developing sub-intestinal vessels. Angiogenesis inhibitors added to the fish water or to the injected cell suspension prevent tumor-induced neovascularization. Also, gene inactivation by antisense morpholino oligonucleotide injection in zebrafish embryos may allow the rapid identification of genes involved in tumor Angiogenesis. The Assay represents a novel tool for investigating tumor Angiogenesis and for antiangiogenic drug discovery.

Stefania Nicoli - One of the best experts on this subject based on the ideXlab platform.

  • fibroblast growth factor 2 induced Angiogenesis in zebrafish the zebrafish yolk membrane zfym Angiogenesis Assay
    Journal of Cellular and Molecular Medicine, 2009
    Co-Authors: Stefania Nicoli, Giulia De Sena, Marco Presta
    Abstract:

    Angiogenesis plays a key role in tumour growth and metastasis. The teleost zebrafish (Danio rerio) represents a promising alternative model in cancer research. Here, we describe a zebrafish yolk membrane (ZFYM) Angiogenesis Assays based on the injection of 1–30 ng of human recombinant FGF2 (rFGF2) in the perivitelline space of zebrafish embryos in the proximity of developing subintestinal vein vessels (SIVs) at 48 hrs after fertilization. The rFGF2 induces a rapid and dose-dependent angiogenic response from the SIV basket, characterized by the ectopic growth of newly formed, alkaline phosphatase-positive blood vessels. These vessels are formed by proliferating cells that incorporate bromodeoxyuridine and express the endothelial cell markers vegfr2/kdr and fli1. Microangiography shows that rFGF2-induced vessels are patent and connected to the systemic circulation of the embryo. In keeping with these observations, fli1:EGFP+ cells isolated from transgenic tg(fli1:EGFP)y1 zebrafish embryos express the tyrosine kinase (TK) FGF receptor-1 (FGFR1) and activate extracellular signal-regulated kinase signalling when stimulated in vitro by rFGF2. The low molecular weight TK-FGFR1 inhibitor SU5402 and the high molecular weight FGF2 antagonist long-pentraxin 3 inhibit the angiogenic activity of rFGF2 when added to fish water or when co-injected with the growth factor, respectively. Moreover, similar to rFGF2, injection of the zebrafish form of vascular endothelial growth factor-A (VEGF-A) induces a significant angiogenic response in the ZFYM Assay that is suppressed by the VEGF receptor-2/KDR TK inhibitor SU5416. The ZFYM Assay represents a novel tool for testing the activity of low and high molecular weight inhibitors targeting a defined angiogenic growth factor in zebrafish. The Assay may offer significant advantages when compared to other animal models.

  • the zebrafish tumor xenograft Angiogenesis Assay
    Nature Protocols, 2007
    Co-Authors: Stefania Nicoli, Marco Presta
    Abstract:

    Zebrafish (Danio rerio) represents a powerful model system in cancer research. Recent observations have shown the possibility to exploit zebrafish to investigate tumor Angiogenesis, a pivotal step in cancer progression and target for anti-tumor therapies. Novel genetic tools and high resolutionin vivo imaging techniques are also becoming available in zebrafish. It is anticipated that zebrafish will represent an important tool for chemical discovery and gene targeting in tumor Angiogenesis. Here we describe a method to study tumor Angiogenesis in zebrafish (Danio rerio) based on the injection of proangiogenic mammalian tumor cells into the perivitelline space of zebrafish embryos at 48 h post-fertilization. Within 24–48 h, proangiogenic tumor grafts induce a neovascular response originating from the developing sub-intestinal vessels. Angiogenesis inhibitors added to the fish water or to the injected cell suspension prevent tumor-induced neovascularization. Also, gene inactivation by antisense morpholino oligonucleotide injection in zebrafish embryos may allow the rapid identification of genes involved in tumor Angiogenesis. The Assay represents a novel tool for investigating tumor Angiogenesis and for antiangiogenic drug discovery.

Kou-gi Shyu - One of the best experts on this subject based on the ideXlab platform.

  • chrysin suppresses il 6 induced Angiogenesis via down regulation of jak1 stat3 and vegf an in vitro and in ovo approach
    Journal of Agricultural and Food Chemistry, 2010
    Co-Authors: Kou-gi Shyu, Bao Wei Wang, Hang Chang, Yenhsu Chen, Jen Hwey Chiu
    Abstract:

    Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced Angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane Assay, an in ovo Angiogenesis Assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiAngiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced Angiogenesis through modulation of the sIL-6R/gp130/JAK...

  • chrysin suppresses il 6 induced Angiogenesis via down regulation of jak1 stat3 and vegf an in vitro and in ovo approach
    Journal of Agricultural and Food Chemistry, 2010
    Co-Authors: Chiu Mei Lin, Bao Wei Wang, Hang Chang, Yenhsu Chen, Kou-gi Shyu, Jen Hwey Chiu
    Abstract:

    Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced Angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane Assay, an in ovo Angiogenesis Assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiAngiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced Angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signaling pathway. Chrysin may provide new therapeutic potential for IL-6-induced pathological Angiogenesis.

Incheol Kang - One of the best experts on this subject based on the ideXlab platform.

  • high throughput screening of novel peptide inhibitors of an integrin receptor from the hexapeptide library by using a protein microarray chip
    Journal of Biomolecular Screening, 2004
    Co-Authors: Dongku Kang, Soo-ik Chang, Incheol Kang
    Abstract:

    3Protein microarray is an emerging technology that makes high-throughput analysis possible for protein-protein interactions and analysis of proteome and biomarkers in parallel. The authors investigated the application of a novel protein microarray chip, ProteoChip, in new drug discovery. Integrin αvβ3 microarray immobilized on the ProteoChip was employed to screen new active peptides against the integrin from multiple hexapeptide sublibraries of a positional scanning synthetic peptide combinatorial library (PS-SPCL). The integrin αvβ3-vitronectin interaction was successfully demonstrated on the integrin microarray in a dose-dependent manner and was inhibited not only by the synthetic RGD peptide but also by various integrin antagonists on the integrin microarray chip. Novel peptide ligands with high affinity to the integrin were also identified from the peptide libraries with this chip-based screening system by a competitive inhibition Assay in a simultaneous and highthroughput fashion. The authors have confirmed antiangiogenic functions of the novel peptides thus screened through an in vitro and in vivo Angiogenesis Assay. These results provide evidence that the ProteoChip is a promising tool for highthroughput screening of lead molecules in new drug development. (Journal of Biomolecular Screening 2004:687-694)

  • high throughput screening of novel peptide inhibitors of an integrin receptor from the hexapeptide library by using a protein microarray chip
    Journal of Biomolecular Screening, 2004
    Co-Authors: Yoonsuk Lee, Dongku Kang, Soo-ik Chang, Moon Hi Han, Incheol Kang
    Abstract:

    Protein microarray is an emerging technology that makes high-throughput analysis possible for protein-protein interactions and analysis of proteome and biomarkers in parallel. The authors investigated the application of a novel protein microarray chip, ProteoChip, in new drug discovery. Integrin alpha(v)beta(3) microarray immobilized on the ProteoChip was employed to screen new active peptides against the integrin from multiple hexapeptide sublibraries of a positional scanning synthetic peptide combinatorial library (PS-SPCL). The integrin alpha(v)beta(3)-vitronectin interaction was successfully demonstrated on the integrin microarray in a dose-dependent manner and was inhibited not only by the synthetic RGD peptide but also by various integrin antagonists on the integrin microarray chip. Novel peptide ligands with high affinity to the integrin were also identified from the peptide libraries with this chip-based screening system by a competitive inhibition Assay in a simultaneous and high-throughput fashion. The authors have confirmed antiangiogenic functions of the novel peptides thus screened through an in vitro and in vivo Angiogenesis Assay. These results provide evidence that the ProteoChip is a promising tool for high-throughput screening of lead molecules in new drug development.