The Experts below are selected from a list of 11481 Experts worldwide ranked by ideXlab platform
Yonggang Zhou - One of the best experts on this subject based on the ideXlab platform.
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expression of a recombinant hybrid antimicrobial peptide magainin ii cecropin b in the mycelium of the medicinal fungus cordyceps militaris and its validation in mice
Microbial Cell Factories, 2018Co-Authors: Min Zhang, Yuanlong Shan, Hongtao Gao, Bin Wang, Xin Liu, Yuanyuan Dong, Xiuming Liu, Na Yao, Yonggang ZhouAbstract:Antibiotic residues can cause antibiotic resistance in livestock and their food safety-related issues have increased the consumer demand for products lacking these residues. Hence, developing safe and effective antibiotic alternatives is important to the Animal Feed Industry. With their strong antibacterial actions, antimicrobial peptides have potential as antibiotic alternatives. We investigated the antibacterial and immunomodulatory activities and the mechanisms of action of an antimicrobial peptide. The hybrid antimicrobial peptide magainin II-cecropin B (Mag II-CB) gene was transformed into the medicinal Cordyceps militaris fungus. Recombinant Mag II-CB exhibited broad-spectrum antibacterial activity in vitro and its antibacterial and immunomodulatory functions were evaluated in BALB/c mice infected with Escherichia coli (ATCC 25922). Histologically, Mag II-CB ameliorated E. coli-related intestinal damage and maintained the integrity of the intestinal mucosal barrier by up-regulating tight junction proteins (zonula occludens-1, claudin-1 and occludin). The intestinal microbial flora was positively modulated in the Mag II-CB-treated mice infected with E. coli. Mag II-CB treatment also supported immune functioning in the mice by regulating their plasma immunoglobulin and ileum secreted immunoglobulin A levels, by attenuating their pro-inflammatory cytokine levels, and by elevating their anti-inflammatory cytokines levels. Moreover, directly Feeding the infected mice with the C. militaris mycelium producing Mag II-CB further proofed the antibacterial and immunomodulatory functions of recombinant hybrid antimicrobial peptide. Our findings suggest that both purified recombinant AMPs and C. militaris mycelium producing AMPs display antibacterial and immunomodulatory activities in mice. And C. militaris producing AMPs has the potential to become a substitute to antibiotics as a Feed additive for livestock in future.
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Expression of a recombinant hybrid antimicrobial peptide magainin II-cecropin B in the mycelium of the medicinal fungus Cordyceps militaris and its validation in mice
BMC, 2018Co-Authors: Min Zhang, Yuanlong Shan, Hongtao Gao, Bin Wang, Xin Liu, Yuanyuan Dong, Xiuming Liu, Na Yao, Yonggang ZhouAbstract:Abstract Background Antibiotic residues can cause antibiotic resistance in livestock and their food safety-related issues have increased the consumer demand for products lacking these residues. Hence, developing safe and effective antibiotic alternatives is important to the Animal Feed Industry. With their strong antibacterial actions, antimicrobial peptides have potential as antibiotic alternatives. Results We investigated the antibacterial and immunomodulatory activities and the mechanisms of action of an antimicrobial peptide. The hybrid antimicrobial peptide magainin II-cecropin B (Mag II-CB) gene was transformed into the medicinal Cordyceps militaris fungus. Recombinant Mag II-CB exhibited broad-spectrum antibacterial activity in vitro and its antibacterial and immunomodulatory functions were evaluated in BALB/c mice infected with Escherichia coli (ATCC 25922). Histologically, Mag II-CB ameliorated E. coli-related intestinal damage and maintained the integrity of the intestinal mucosal barrier by up-regulating tight junction proteins (zonula occludens-1, claudin-1 and occludin). The intestinal microbial flora was positively modulated in the Mag II-CB-treated mice infected with E. coli. Mag II-CB treatment also supported immune functioning in the mice by regulating their plasma immunoglobulin and ileum secreted immunoglobulin A levels, by attenuating their pro-inflammatory cytokine levels, and by elevating their anti-inflammatory cytokines levels. Moreover, directly Feeding the infected mice with the C. militaris mycelium producing Mag II-CB further proofed the antibacterial and immunomodulatory functions of recombinant hybrid antimicrobial peptide. Conclusion Our findings suggest that both purified recombinant AMPs and C. militaris mycelium producing AMPs display antibacterial and immunomodulatory activities in mice. And C. militaris producing AMPs has the potential to become a substitute to antibiotics as a Feed additive for livestock in future
Lilia Renee Cavaglieri - One of the best experts on this subject based on the ideXlab platform.
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impact of mycotoxin contamination in the Animal Feed Industry
Current opinion in food science, 2019Co-Authors: Alejandra Paola Magnoli, Valeria Lorena Poloni, Lilia Renee CavaglieriAbstract:Mycotoxin contamination generally affects a large number of raw materials and finished Feed intended for Animal production. The economic impact on livestock production encompasses both, the cost of eliminating contaminated Feed and the reduction in Animal productivity. Therefore, the increase in veterinary care costs and technical efforts aimed at reducing mycotoxins negative effects. Cereals and by-products constitute the main ingredients of finished Feed in production Animals. Food processing affects mycotoxin distribution and concentration. The mycotoxin distribution in cereal processing procedures is a worldwide problem due to the high economic and health impacts. The use of a safe diet in which the risk of mycotoxin contamination can be minimized and the cost/benefit is accurately quantified should allow the maximization of herd productivity. The present review intends to address the problem of Animal Feed contamination describing the impact on Animal health and productivity. Moreover, the economic impact generated by mycotoxins and their control in the Animal Feed Industry was considered.
Min Zhang - One of the best experts on this subject based on the ideXlab platform.
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expression of a recombinant hybrid antimicrobial peptide magainin ii cecropin b in the mycelium of the medicinal fungus cordyceps militaris and its validation in mice
Microbial Cell Factories, 2018Co-Authors: Min Zhang, Yuanlong Shan, Hongtao Gao, Bin Wang, Xin Liu, Yuanyuan Dong, Xiuming Liu, Na Yao, Yonggang ZhouAbstract:Antibiotic residues can cause antibiotic resistance in livestock and their food safety-related issues have increased the consumer demand for products lacking these residues. Hence, developing safe and effective antibiotic alternatives is important to the Animal Feed Industry. With their strong antibacterial actions, antimicrobial peptides have potential as antibiotic alternatives. We investigated the antibacterial and immunomodulatory activities and the mechanisms of action of an antimicrobial peptide. The hybrid antimicrobial peptide magainin II-cecropin B (Mag II-CB) gene was transformed into the medicinal Cordyceps militaris fungus. Recombinant Mag II-CB exhibited broad-spectrum antibacterial activity in vitro and its antibacterial and immunomodulatory functions were evaluated in BALB/c mice infected with Escherichia coli (ATCC 25922). Histologically, Mag II-CB ameliorated E. coli-related intestinal damage and maintained the integrity of the intestinal mucosal barrier by up-regulating tight junction proteins (zonula occludens-1, claudin-1 and occludin). The intestinal microbial flora was positively modulated in the Mag II-CB-treated mice infected with E. coli. Mag II-CB treatment also supported immune functioning in the mice by regulating their plasma immunoglobulin and ileum secreted immunoglobulin A levels, by attenuating their pro-inflammatory cytokine levels, and by elevating their anti-inflammatory cytokines levels. Moreover, directly Feeding the infected mice with the C. militaris mycelium producing Mag II-CB further proofed the antibacterial and immunomodulatory functions of recombinant hybrid antimicrobial peptide. Our findings suggest that both purified recombinant AMPs and C. militaris mycelium producing AMPs display antibacterial and immunomodulatory activities in mice. And C. militaris producing AMPs has the potential to become a substitute to antibiotics as a Feed additive for livestock in future.
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Expression of a recombinant hybrid antimicrobial peptide magainin II-cecropin B in the mycelium of the medicinal fungus Cordyceps militaris and its validation in mice
BMC, 2018Co-Authors: Min Zhang, Yuanlong Shan, Hongtao Gao, Bin Wang, Xin Liu, Yuanyuan Dong, Xiuming Liu, Na Yao, Yonggang ZhouAbstract:Abstract Background Antibiotic residues can cause antibiotic resistance in livestock and their food safety-related issues have increased the consumer demand for products lacking these residues. Hence, developing safe and effective antibiotic alternatives is important to the Animal Feed Industry. With their strong antibacterial actions, antimicrobial peptides have potential as antibiotic alternatives. Results We investigated the antibacterial and immunomodulatory activities and the mechanisms of action of an antimicrobial peptide. The hybrid antimicrobial peptide magainin II-cecropin B (Mag II-CB) gene was transformed into the medicinal Cordyceps militaris fungus. Recombinant Mag II-CB exhibited broad-spectrum antibacterial activity in vitro and its antibacterial and immunomodulatory functions were evaluated in BALB/c mice infected with Escherichia coli (ATCC 25922). Histologically, Mag II-CB ameliorated E. coli-related intestinal damage and maintained the integrity of the intestinal mucosal barrier by up-regulating tight junction proteins (zonula occludens-1, claudin-1 and occludin). The intestinal microbial flora was positively modulated in the Mag II-CB-treated mice infected with E. coli. Mag II-CB treatment also supported immune functioning in the mice by regulating their plasma immunoglobulin and ileum secreted immunoglobulin A levels, by attenuating their pro-inflammatory cytokine levels, and by elevating their anti-inflammatory cytokines levels. Moreover, directly Feeding the infected mice with the C. militaris mycelium producing Mag II-CB further proofed the antibacterial and immunomodulatory functions of recombinant hybrid antimicrobial peptide. Conclusion Our findings suggest that both purified recombinant AMPs and C. militaris mycelium producing AMPs display antibacterial and immunomodulatory activities in mice. And C. militaris producing AMPs has the potential to become a substitute to antibiotics as a Feed additive for livestock in future
T Satyanarayana - One of the best experts on this subject based on the ideXlab platform.
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a cost effective cane molasses medium for enhanced cell bound phytase production by pichia anomala
Journal of Applied Microbiology, 2004Co-Authors: A Vohra, T SatyanarayanaAbstract:Aim: Formulation of an inexpensive cane molasses medium for improved cell-bound phytase production by Pichia anomala. Methods and Results: Cell-bound phytase production by Pichia anomala was compared in synthetic glucose–beef extract and cane molasses media. The yeast was cultivated in 250 ml flasks containing 50 ml of the medium, inoculated with a 12 h-old inoculum (3 × 106 CFU ml−1) and incubated at 25°C for 24 h at 250 rev min−1. Different cultural parameters were optimized in cane molasses medium in batch fermentation. The cell-bound phytase content increased significantly in cane molasses medium (176 U g−1 dry biomass) when compared with the synthetic medium (100 U g−1 dry biomass). In fed-batch fermentation, a marked increase in biomass (20 g l−1) and the phytase yield (3000 U l−1) were recorded in cane molasses medium. The cost of production in cane molasses medium was £0·006 per 1000 U, which is much lower when compared with that in synthetic medium (£0·25 per 1000 U). Conclusions: An overall 86·6% enhancement in phytase yield was attained in optimized cane molasses medium using fed-batch fermentation when compared with that in synthetic medium. Furthermore, the production in cane molasses medium is cost-effective. Significance and Impact of the Study: Phytase yield was improved in cane molasses when compared with the synthetic medium, and the cost of production was also significantly reduced. This enzyme can find application in the Animal Feed Industry for improving the nutritional status of Feed and combating environmental pollution.
Alejandra Paola Magnoli - One of the best experts on this subject based on the ideXlab platform.
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impact of mycotoxin contamination in the Animal Feed Industry
Current opinion in food science, 2019Co-Authors: Alejandra Paola Magnoli, Valeria Lorena Poloni, Lilia Renee CavaglieriAbstract:Mycotoxin contamination generally affects a large number of raw materials and finished Feed intended for Animal production. The economic impact on livestock production encompasses both, the cost of eliminating contaminated Feed and the reduction in Animal productivity. Therefore, the increase in veterinary care costs and technical efforts aimed at reducing mycotoxins negative effects. Cereals and by-products constitute the main ingredients of finished Feed in production Animals. Food processing affects mycotoxin distribution and concentration. The mycotoxin distribution in cereal processing procedures is a worldwide problem due to the high economic and health impacts. The use of a safe diet in which the risk of mycotoxin contamination can be minimized and the cost/benefit is accurately quantified should allow the maximization of herd productivity. The present review intends to address the problem of Animal Feed contamination describing the impact on Animal health and productivity. Moreover, the economic impact generated by mycotoxins and their control in the Animal Feed Industry was considered.