The Experts below are selected from a list of 2223 Experts worldwide ranked by ideXlab platform
Keith G. Davies - One of the best experts on this subject based on the ideXlab platform.
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Cuticle surface coat of plant-Parasitic Nematodes.
Annual Review of Phytopathology, 2011Co-Authors: Keith G. Davies, Rosane H. C. CurtisAbstract:The surface coat (SC) of the plant-Parasitic nematode cuticle is an understudied area of current research, even though it likely plays key roles in both nematode-plant and nematode-microbe interactions. Although in several ways Caenorhabditis elegans is a poor model for plant-Parasitic Nematodes, it is a useful starting point for investigations of the cuticle and its SC, especially in the light of recent work using this species as a model for innate immunity and the generic biology underpinning much host-parasite biology. We review the research focused on the involvement of the SC of plant-Parasitic Nematodes. Using the insights gained from Animal-Parasitic Nematodes and other sequenced Nematodes, we discuss the key roles that the SC may play.
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predatory behaviour of trapping fungi against srf mutants of caenorhabditis elegans and different plant and Animal Parasitic Nematodes
Parasitology, 1999Co-Authors: Mendoza P De Gives, Keith G. Davies, S J Clark, Jerzy M. BehnkeAbstract:The initial infection process of nematode-trapping fungi is based on an interaction between the trapping structure of the fungus and the surface of the nematode cuticle. A bioassay was designed to investigate the predatory response of several isolates of nematode-trapping fungi against 3 mutants of Caenorhabditis elegans (AT6, AT10 and CL261), which have been reported to differ in the reaction of their cuticle to antibodies and lectins. The bioassay was also applied to infective larvae of Animal (Haemonchus contortus, Teladorsagia (Ostertagia) circumcincta and Trichostrongylus axei) and plant (Meloidogyne spp.) Parasitic Nematodes. Differences in trapping ability were most marked in the first 24 h, and were density dependent. Although the isolate of Arthrobotrys responded very rapidly in the first 24 h, Duddingtonia flagrans was generally the most effective isolate and Monacrosporium responded relatively poorly throughout all experiments. All the fungi tested trapped the srf mutants of C. elegans more efficiently than the wild type, and there were differences between the different srf mutants of C. elegans. Differences in trapping ability were also observed between different isolates of D. flagrans; similarly, differences in trapping behaviour were observed not only amongst the different species of plant-Parasitic Nematodes, but also between the sheathed and exsheathed larvae of the Animal-Parasitic Nematodes.
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Attachment tests of Pasteuria penetrans to the cuticle of plant and Animal Parasitic Nematodes, free living Nematodes and srf mutants of Caenorhabditis elegans.
Journal of helminthology, 1999Co-Authors: P. Mendoza De Gives, Keith G. Davies, Mari Morgan, Jerzy M. BehnkeAbstract:Populations of Pasteuria penetrans isolated from root-knot Nematodes (Meloidogyne spp.) and cyst Nematodes (Heterodera spp.) were tested for their ability to adhere to a limited selection of sheathed and ex-sheathed Animal Parasitic Nematodes, free living Nematodes, including Caenorhabditis elegans wild type and several srf mutants, and plant Parasitic Nematodes. The attachment of spores of Pasteuria was restricted and no spores were observed adhering to any of the Animal Parasitic Nematodes either with or without their sheath or to any of the free living Nematodes including C. elegans and the srf mutants. All spore attachment was restricted to plant Parasitic Nematodes; however, spores isolated from cyst Nematodes showed the ability to adhere to other genera of plant Parasitic Nematodes which was not the case with spores isolated from root-knot Nematodes. The results are discussed in relationship to cuticular heterogeneity.
Jerzy M. Behnke - One of the best experts on this subject based on the ideXlab platform.
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predatory behaviour of trapping fungi against srf mutants of caenorhabditis elegans and different plant and Animal Parasitic Nematodes
Parasitology, 1999Co-Authors: Mendoza P De Gives, Keith G. Davies, S J Clark, Jerzy M. BehnkeAbstract:The initial infection process of nematode-trapping fungi is based on an interaction between the trapping structure of the fungus and the surface of the nematode cuticle. A bioassay was designed to investigate the predatory response of several isolates of nematode-trapping fungi against 3 mutants of Caenorhabditis elegans (AT6, AT10 and CL261), which have been reported to differ in the reaction of their cuticle to antibodies and lectins. The bioassay was also applied to infective larvae of Animal (Haemonchus contortus, Teladorsagia (Ostertagia) circumcincta and Trichostrongylus axei) and plant (Meloidogyne spp.) Parasitic Nematodes. Differences in trapping ability were most marked in the first 24 h, and were density dependent. Although the isolate of Arthrobotrys responded very rapidly in the first 24 h, Duddingtonia flagrans was generally the most effective isolate and Monacrosporium responded relatively poorly throughout all experiments. All the fungi tested trapped the srf mutants of C. elegans more efficiently than the wild type, and there were differences between the different srf mutants of C. elegans. Differences in trapping ability were also observed between different isolates of D. flagrans; similarly, differences in trapping behaviour were observed not only amongst the different species of plant-Parasitic Nematodes, but also between the sheathed and exsheathed larvae of the Animal-Parasitic Nematodes.
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Attachment tests of Pasteuria penetrans to the cuticle of plant and Animal Parasitic Nematodes, free living Nematodes and srf mutants of Caenorhabditis elegans.
Journal of helminthology, 1999Co-Authors: P. Mendoza De Gives, Keith G. Davies, Mari Morgan, Jerzy M. BehnkeAbstract:Populations of Pasteuria penetrans isolated from root-knot Nematodes (Meloidogyne spp.) and cyst Nematodes (Heterodera spp.) were tested for their ability to adhere to a limited selection of sheathed and ex-sheathed Animal Parasitic Nematodes, free living Nematodes, including Caenorhabditis elegans wild type and several srf mutants, and plant Parasitic Nematodes. The attachment of spores of Pasteuria was restricted and no spores were observed adhering to any of the Animal Parasitic Nematodes either with or without their sheath or to any of the free living Nematodes including C. elegans and the srf mutants. All spore attachment was restricted to plant Parasitic Nematodes; however, spores isolated from cyst Nematodes showed the ability to adhere to other genera of plant Parasitic Nematodes which was not the case with spores isolated from root-knot Nematodes. The results are discussed in relationship to cuticular heterogeneity.
Keqin Zhang - One of the best experts on this subject based on the ideXlab platform.
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the complete mitochondrial genomes of the nematode trapping fungus arthrobotrys oligospora
Mitochondrial DNA Part B, 2018Co-Authors: Lili Jiang, Yunrun Zhang, Keqin Zhang, Ying ZhangAbstract:AbstractArthrobotrys oligospora is a potential candidate of biocontrol agents against plant and Animal Parasitic Nematodes. In this study, the complete mitochondrial genome of A.oligospora was sequenced. This mitogenome is a circular molecule of 160,613 bp in length. Gene annotation showed that 44 putative protein-coding genes and 24 tRNAs, all located on the same strand. The evolutionary relationships between A. oligospora and other representative ascomycetes were revealed based on sequences at the 14 concatenated mitochondrial protein-coding genes.
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genetic diversity and recombination in natural populations of the nematode trapping fungus arthrobotrys oligospora from china
Ecology and Evolution, 2013Co-Authors: Ying Zhang, Min Qiao, Yang Cao, Keqin ZhangAbstract:Nematophagous fungi can trap and capture Nematodes and other small invertebrates. This unique ability has made them ideal organisms from which to develop biological control agents against plant- and Animal-Parasitic Nematodes. However, effective application of biocontrol agents in the field requires a comprehensive understanding about the ecology and population genetics of the nematophagous fungi in natural environments. Here, we genotyped 228 strains of the nematode-trapping fungus Arthrobotrys oligospora using 12 single nucleotide polymorphic markers located on eight random DNA fragments. The strains were from different ecological niches and geographical regions from China. Our analyses identified that ecological niche separations contributed significantly, whereas geographic separation contributed relatively little to the overall genetic variation in our samples of A. oligospora. Interestingly, populations from stressful environments seemed to be more variable and showed more evidence for recombination than those from benign environments at the same geographic areas. We discussed the implications of our results to the conservation and biocontrol application of A. oligospora in agriculture and forestry.
Jeremy M Foster - One of the best experts on this subject based on the ideXlab platform.
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interdomain lateral gene transfer of an essential ferrochelatase gene in human Parasitic Nematodes
Proceedings of the National Academy of Sciences of the United States of America, 2013Co-Authors: Bo Wu, Harry A. Dailey, Jacopo F Novelli, Daojun Jiang, Frederic Landmann, Louise Ford, Mark J Taylor, Clotilde K S Carlow, Sanjay Kumar, Jeremy M FosterAbstract:Lateral gene transfer events between bacteria and Animals highlight an avenue for evolutionary genomic loss/gain of function. Herein, we report functional lateral gene transfer in Animal Parasitic Nematodes. Members of the Nematoda are heme auxotrophs, lacking the ability to synthesize heme; however, the human filarial parasite Brugia malayi has acquired a bacterial gene encoding ferrochelatase (BmFeCH), the terminal step in heme biosynthesis. BmFeCH, encoded by a 9-exon gene, is a mitochondrial-targeted, functional ferrochelatase based on enzyme assays, complementation, and inhibitor studies. Homologs have been identified in several filariae and a nonfilarial nematode. RNAi and ex vivo inhibitor experiments indicate that BmFeCH is essential for viability, validating it as a potential target for filariasis control.
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efficient in vitro rna interference and immunofluorescence based phenotype analysis in a human Parasitic nematode brugia malayi
Parasites & Vectors, 2012Co-Authors: Frederic Landmann, Jeremy M Foster, Barton E Slatko, William J SullivanAbstract:Background RNA interference (RNAi) is an efficient reverse genetics technique for investigating gene function in eukaryotes. The method has been widely used in model organisms, such as the free-living nematode Caenorhabditis elegans, where it has been deployed in genome-wide high throughput screens to identify genes involved in many cellular and developmental processes. However, RNAi techniques have not translated efficiently to Animal Parasitic Nematodes that afflict humans, livestock and companion Animals across the globe, creating a dependency on data tentatively inferred from C. elegans.
Frederic Landmann - One of the best experts on this subject based on the ideXlab platform.
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interdomain lateral gene transfer of an essential ferrochelatase gene in human Parasitic Nematodes
Proceedings of the National Academy of Sciences of the United States of America, 2013Co-Authors: Bo Wu, Harry A. Dailey, Jacopo F Novelli, Daojun Jiang, Frederic Landmann, Louise Ford, Mark J Taylor, Clotilde K S Carlow, Sanjay Kumar, Jeremy M FosterAbstract:Lateral gene transfer events between bacteria and Animals highlight an avenue for evolutionary genomic loss/gain of function. Herein, we report functional lateral gene transfer in Animal Parasitic Nematodes. Members of the Nematoda are heme auxotrophs, lacking the ability to synthesize heme; however, the human filarial parasite Brugia malayi has acquired a bacterial gene encoding ferrochelatase (BmFeCH), the terminal step in heme biosynthesis. BmFeCH, encoded by a 9-exon gene, is a mitochondrial-targeted, functional ferrochelatase based on enzyme assays, complementation, and inhibitor studies. Homologs have been identified in several filariae and a nonfilarial nematode. RNAi and ex vivo inhibitor experiments indicate that BmFeCH is essential for viability, validating it as a potential target for filariasis control.
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efficient in vitro rna interference and immunofluorescence based phenotype analysis in a human Parasitic nematode brugia malayi
Parasites & Vectors, 2012Co-Authors: Frederic Landmann, Jeremy M Foster, Barton E Slatko, William J SullivanAbstract:Background RNA interference (RNAi) is an efficient reverse genetics technique for investigating gene function in eukaryotes. The method has been widely used in model organisms, such as the free-living nematode Caenorhabditis elegans, where it has been deployed in genome-wide high throughput screens to identify genes involved in many cellular and developmental processes. However, RNAi techniques have not translated efficiently to Animal Parasitic Nematodes that afflict humans, livestock and companion Animals across the globe, creating a dependency on data tentatively inferred from C. elegans.