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Silvano Focardi - One of the best experts on this subject based on the ideXlab platform.

  • potential role of cholinesterases in the invasive capacity of the freshwater bivalve Anodonta woodiana bivalvia unionacea a comparative study with the indigenous species of the genus Anodonta sp
    Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (p < 0.05) using acetylthiocholine (ASCh) as substrate and the activity was more widely distributed in tissues involved in movement (adductor muscle and foot), respiration, feeding (gills) and reproduction (gonads). Moreover, only the invasive species, A. woodiana, showed detectable ChE (vs. ASCh) activity in gill tissue. No substrate specificity was observed in any tissue of either species as already described for other bivalve species. ChE activity was not inhibited by Iso-OMPA but showed high sensitivity to BW248c51 and eserine. Both species showed moderate to low sensitivities in vitro to OP insecticides in the range 10− 7–10− 2 M. Calculated IC50 for fenitrothion and chlorpyrifos was in the range 10− 6–10− 3 M in muscle of A. woodiana while a higher inhibition was observed for fenitrothion (10− 7 M) and lower for chlorpyrifos (10− 2 M) in the indigenous species Anodonta sp. Similar IC50 of 10− 5–10− 6 M were observed for DFP and azamethiphos in both species. The hypotheses of other authors that acetylcholinesterase (AChE) is involved in the control of many essential functions, such as frontal ciliary activity of gill epithelium, temperature resistance, ciliary activity for transport of suspended particulate, valve opening and embryo development, suggest that the high catalytic efficiency of the invasive species may endow it with a competitive advantage over the endemic species. In view of the peculiar reproductive strategy of these mussels, higher ChE vs. ASCh activity in gonads of the invasive species could also favour glochidium production and embryo development under a wider range of environmental conditions.

  • Potential role of cholinesterases in the invasive capacity of the freshwater bivalve, Anodonta woodiana (Bivalvia: Unionacea): a comparative study with the indigenous species of the genus, Anodonta sp.
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (p

  • A potential role of cholinesterases in the invasive capacity of the freshwater bivalve Anodonta woodiana (Lea, 1834) (Bivalvia: unionacea): a comparative study with the indigenous species of the genus Anodonta sp. (Linnaeus, 1758).
    2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (pb0.05) using acetylthiocholine (ASCh) as substrate and the activity was more widely distributed in tissues involved in movement (adductor muscle and foot), respiration, feeding (gills) and reproduction (gonads). Moreover, only the invasive species, A. woodiana, showed detectable ChE (vs. ASCh) activity in gill tissue. No substrate specificity was observed in any tissue of either species as already described for other bivalve species. ChE activity was not inhibited by Iso-OMPA but showed high sensitivity to BW248c51 and eserine. Both species showed moderate to low sensitivities in vitro to OP insecticides in the range 10−7–10−2 M. Calculated IC50 for fenitrothion and chlorpyrifos was in the range 10−6–10−3 M in muscle of A. woodiana while a higher inhibition was observed for fenitrothion (10−7 M) and lower for chlorpyrifos (10−2 M) in the indigenous species Anodonta sp. Similar IC50 of 10−5–10−6 M were observed for DFP and azamethiphos in both species. The hypotheses of other authors that acetylcholinesterase (AChE) is involved in the control of many essential functions, such as frontal ciliary activity of gill epithelium, temperature resistance, ciliary activity for transport of suspended particulate, valve opening and embryo development, suggest that the high catalytic efficiency of the invasive species may endow it with a competitive advantage over the endemic species. In view of the peculiar reproductive strategy of these mussels, higher ChE vs. ASCh activity in gonads of the invasive species could also favour glochidium production and embryo development under a wider range of environmental conditions

Ilaria Corsi - One of the best experts on this subject based on the ideXlab platform.

  • potential role of cholinesterases in the invasive capacity of the freshwater bivalve Anodonta woodiana bivalvia unionacea a comparative study with the indigenous species of the genus Anodonta sp
    Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (p < 0.05) using acetylthiocholine (ASCh) as substrate and the activity was more widely distributed in tissues involved in movement (adductor muscle and foot), respiration, feeding (gills) and reproduction (gonads). Moreover, only the invasive species, A. woodiana, showed detectable ChE (vs. ASCh) activity in gill tissue. No substrate specificity was observed in any tissue of either species as already described for other bivalve species. ChE activity was not inhibited by Iso-OMPA but showed high sensitivity to BW248c51 and eserine. Both species showed moderate to low sensitivities in vitro to OP insecticides in the range 10− 7–10− 2 M. Calculated IC50 for fenitrothion and chlorpyrifos was in the range 10− 6–10− 3 M in muscle of A. woodiana while a higher inhibition was observed for fenitrothion (10− 7 M) and lower for chlorpyrifos (10− 2 M) in the indigenous species Anodonta sp. Similar IC50 of 10− 5–10− 6 M were observed for DFP and azamethiphos in both species. The hypotheses of other authors that acetylcholinesterase (AChE) is involved in the control of many essential functions, such as frontal ciliary activity of gill epithelium, temperature resistance, ciliary activity for transport of suspended particulate, valve opening and embryo development, suggest that the high catalytic efficiency of the invasive species may endow it with a competitive advantage over the endemic species. In view of the peculiar reproductive strategy of these mussels, higher ChE vs. ASCh activity in gonads of the invasive species could also favour glochidium production and embryo development under a wider range of environmental conditions.

  • Potential role of cholinesterases in the invasive capacity of the freshwater bivalve, Anodonta woodiana (Bivalvia: Unionacea): a comparative study with the indigenous species of the genus, Anodonta sp.
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (p

  • A potential role of cholinesterases in the invasive capacity of the freshwater bivalve Anodonta woodiana (Lea, 1834) (Bivalvia: unionacea): a comparative study with the indigenous species of the genus Anodonta sp. (Linnaeus, 1758).
    2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (pb0.05) using acetylthiocholine (ASCh) as substrate and the activity was more widely distributed in tissues involved in movement (adductor muscle and foot), respiration, feeding (gills) and reproduction (gonads). Moreover, only the invasive species, A. woodiana, showed detectable ChE (vs. ASCh) activity in gill tissue. No substrate specificity was observed in any tissue of either species as already described for other bivalve species. ChE activity was not inhibited by Iso-OMPA but showed high sensitivity to BW248c51 and eserine. Both species showed moderate to low sensitivities in vitro to OP insecticides in the range 10−7–10−2 M. Calculated IC50 for fenitrothion and chlorpyrifos was in the range 10−6–10−3 M in muscle of A. woodiana while a higher inhibition was observed for fenitrothion (10−7 M) and lower for chlorpyrifos (10−2 M) in the indigenous species Anodonta sp. Similar IC50 of 10−5–10−6 M were observed for DFP and azamethiphos in both species. The hypotheses of other authors that acetylcholinesterase (AChE) is involved in the control of many essential functions, such as frontal ciliary activity of gill epithelium, temperature resistance, ciliary activity for transport of suspended particulate, valve opening and embryo development, suggest that the high catalytic efficiency of the invasive species may endow it with a competitive advantage over the endemic species. In view of the peculiar reproductive strategy of these mussels, higher ChE vs. ASCh activity in gonads of the invasive species could also favour glochidium production and embryo development under a wider range of environmental conditions

Lucio Castagnolo - One of the best experts on this subject based on the ideXlab platform.

  • THE REPRODUCTIVE CYCLE AND THE GLOCHIDIUM OF Anodonta CYGNE A L. FROM LAGO TRASIMENO (CENTRAL ITALY)
    Monitore Zoologico Italiano-Italian Journal of Zoology, 2013
    Co-Authors: Folco Giusti, Lucio Castagnolo, L. Moretti Farina, Aristeo Renzoni
    Abstract:

    SUMMARY The different phases of the biological cycle of Anodonta cygnea L. living in lake Trasimeno (Central Italy) are described in the present paper. Researches have been carried out on histological sections of germinal tissues and gills of specimens of both sexes sacrificed every month and verified by following the course of the parasitic phase of life of glochidia on the fish of the lake. Moreover, the various species of fish heavily infested by glochidia have been identified and the glochidium infestation frequency has been determined. The structure of the glochidium shell has been studied with the scanning microscope for a better understanding of the mechanism of attachment to fish and to collect more information in order to compare the species of Anodonta from lake Trasimeno with those of other parts of Europe.

  • potential role of cholinesterases in the invasive capacity of the freshwater bivalve Anodonta woodiana bivalvia unionacea a comparative study with the indigenous species of the genus Anodonta sp
    Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (p < 0.05) using acetylthiocholine (ASCh) as substrate and the activity was more widely distributed in tissues involved in movement (adductor muscle and foot), respiration, feeding (gills) and reproduction (gonads). Moreover, only the invasive species, A. woodiana, showed detectable ChE (vs. ASCh) activity in gill tissue. No substrate specificity was observed in any tissue of either species as already described for other bivalve species. ChE activity was not inhibited by Iso-OMPA but showed high sensitivity to BW248c51 and eserine. Both species showed moderate to low sensitivities in vitro to OP insecticides in the range 10− 7–10− 2 M. Calculated IC50 for fenitrothion and chlorpyrifos was in the range 10− 6–10− 3 M in muscle of A. woodiana while a higher inhibition was observed for fenitrothion (10− 7 M) and lower for chlorpyrifos (10− 2 M) in the indigenous species Anodonta sp. Similar IC50 of 10− 5–10− 6 M were observed for DFP and azamethiphos in both species. The hypotheses of other authors that acetylcholinesterase (AChE) is involved in the control of many essential functions, such as frontal ciliary activity of gill epithelium, temperature resistance, ciliary activity for transport of suspended particulate, valve opening and embryo development, suggest that the high catalytic efficiency of the invasive species may endow it with a competitive advantage over the endemic species. In view of the peculiar reproductive strategy of these mussels, higher ChE vs. ASCh activity in gonads of the invasive species could also favour glochidium production and embryo development under a wider range of environmental conditions.

  • Potential role of cholinesterases in the invasive capacity of the freshwater bivalve, Anodonta woodiana (Bivalvia: Unionacea): a comparative study with the indigenous species of the genus, Anodonta sp.
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (p

  • A potential role of cholinesterases in the invasive capacity of the freshwater bivalve Anodonta woodiana (Lea, 1834) (Bivalvia: unionacea): a comparative study with the indigenous species of the genus Anodonta sp. (Linnaeus, 1758).
    2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (pb0.05) using acetylthiocholine (ASCh) as substrate and the activity was more widely distributed in tissues involved in movement (adductor muscle and foot), respiration, feeding (gills) and reproduction (gonads). Moreover, only the invasive species, A. woodiana, showed detectable ChE (vs. ASCh) activity in gill tissue. No substrate specificity was observed in any tissue of either species as already described for other bivalve species. ChE activity was not inhibited by Iso-OMPA but showed high sensitivity to BW248c51 and eserine. Both species showed moderate to low sensitivities in vitro to OP insecticides in the range 10−7–10−2 M. Calculated IC50 for fenitrothion and chlorpyrifos was in the range 10−6–10−3 M in muscle of A. woodiana while a higher inhibition was observed for fenitrothion (10−7 M) and lower for chlorpyrifos (10−2 M) in the indigenous species Anodonta sp. Similar IC50 of 10−5–10−6 M were observed for DFP and azamethiphos in both species. The hypotheses of other authors that acetylcholinesterase (AChE) is involved in the control of many essential functions, such as frontal ciliary activity of gill epithelium, temperature resistance, ciliary activity for transport of suspended particulate, valve opening and embryo development, suggest that the high catalytic efficiency of the invasive species may endow it with a competitive advantage over the endemic species. In view of the peculiar reproductive strategy of these mussels, higher ChE vs. ASCh activity in gonads of the invasive species could also favour glochidium production and embryo development under a wider range of environmental conditions

Angela Maria Pastore - One of the best experts on this subject based on the ideXlab platform.

  • potential role of cholinesterases in the invasive capacity of the freshwater bivalve Anodonta woodiana bivalvia unionacea a comparative study with the indigenous species of the genus Anodonta sp
    Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (p < 0.05) using acetylthiocholine (ASCh) as substrate and the activity was more widely distributed in tissues involved in movement (adductor muscle and foot), respiration, feeding (gills) and reproduction (gonads). Moreover, only the invasive species, A. woodiana, showed detectable ChE (vs. ASCh) activity in gill tissue. No substrate specificity was observed in any tissue of either species as already described for other bivalve species. ChE activity was not inhibited by Iso-OMPA but showed high sensitivity to BW248c51 and eserine. Both species showed moderate to low sensitivities in vitro to OP insecticides in the range 10− 7–10− 2 M. Calculated IC50 for fenitrothion and chlorpyrifos was in the range 10− 6–10− 3 M in muscle of A. woodiana while a higher inhibition was observed for fenitrothion (10− 7 M) and lower for chlorpyrifos (10− 2 M) in the indigenous species Anodonta sp. Similar IC50 of 10− 5–10− 6 M were observed for DFP and azamethiphos in both species. The hypotheses of other authors that acetylcholinesterase (AChE) is involved in the control of many essential functions, such as frontal ciliary activity of gill epithelium, temperature resistance, ciliary activity for transport of suspended particulate, valve opening and embryo development, suggest that the high catalytic efficiency of the invasive species may endow it with a competitive advantage over the endemic species. In view of the peculiar reproductive strategy of these mussels, higher ChE vs. ASCh activity in gonads of the invasive species could also favour glochidium production and embryo development under a wider range of environmental conditions.

  • Potential role of cholinesterases in the invasive capacity of the freshwater bivalve, Anodonta woodiana (Bivalvia: Unionacea): a comparative study with the indigenous species of the genus, Anodonta sp.
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (p

  • A potential role of cholinesterases in the invasive capacity of the freshwater bivalve Anodonta woodiana (Lea, 1834) (Bivalvia: unionacea): a comparative study with the indigenous species of the genus Anodonta sp. (Linnaeus, 1758).
    2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (pb0.05) using acetylthiocholine (ASCh) as substrate and the activity was more widely distributed in tissues involved in movement (adductor muscle and foot), respiration, feeding (gills) and reproduction (gonads). Moreover, only the invasive species, A. woodiana, showed detectable ChE (vs. ASCh) activity in gill tissue. No substrate specificity was observed in any tissue of either species as already described for other bivalve species. ChE activity was not inhibited by Iso-OMPA but showed high sensitivity to BW248c51 and eserine. Both species showed moderate to low sensitivities in vitro to OP insecticides in the range 10−7–10−2 M. Calculated IC50 for fenitrothion and chlorpyrifos was in the range 10−6–10−3 M in muscle of A. woodiana while a higher inhibition was observed for fenitrothion (10−7 M) and lower for chlorpyrifos (10−2 M) in the indigenous species Anodonta sp. Similar IC50 of 10−5–10−6 M were observed for DFP and azamethiphos in both species. The hypotheses of other authors that acetylcholinesterase (AChE) is involved in the control of many essential functions, such as frontal ciliary activity of gill epithelium, temperature resistance, ciliary activity for transport of suspended particulate, valve opening and embryo development, suggest that the high catalytic efficiency of the invasive species may endow it with a competitive advantage over the endemic species. In view of the peculiar reproductive strategy of these mussels, higher ChE vs. ASCh activity in gonads of the invasive species could also favour glochidium production and embryo development under a wider range of environmental conditions

Antonella Lodde - One of the best experts on this subject based on the ideXlab platform.

  • potential role of cholinesterases in the invasive capacity of the freshwater bivalve Anodonta woodiana bivalvia unionacea a comparative study with the indigenous species of the genus Anodonta sp
    Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (p < 0.05) using acetylthiocholine (ASCh) as substrate and the activity was more widely distributed in tissues involved in movement (adductor muscle and foot), respiration, feeding (gills) and reproduction (gonads). Moreover, only the invasive species, A. woodiana, showed detectable ChE (vs. ASCh) activity in gill tissue. No substrate specificity was observed in any tissue of either species as already described for other bivalve species. ChE activity was not inhibited by Iso-OMPA but showed high sensitivity to BW248c51 and eserine. Both species showed moderate to low sensitivities in vitro to OP insecticides in the range 10− 7–10− 2 M. Calculated IC50 for fenitrothion and chlorpyrifos was in the range 10− 6–10− 3 M in muscle of A. woodiana while a higher inhibition was observed for fenitrothion (10− 7 M) and lower for chlorpyrifos (10− 2 M) in the indigenous species Anodonta sp. Similar IC50 of 10− 5–10− 6 M were observed for DFP and azamethiphos in both species. The hypotheses of other authors that acetylcholinesterase (AChE) is involved in the control of many essential functions, such as frontal ciliary activity of gill epithelium, temperature resistance, ciliary activity for transport of suspended particulate, valve opening and embryo development, suggest that the high catalytic efficiency of the invasive species may endow it with a competitive advantage over the endemic species. In view of the peculiar reproductive strategy of these mussels, higher ChE vs. ASCh activity in gonads of the invasive species could also favour glochidium production and embryo development under a wider range of environmental conditions.

  • Potential role of cholinesterases in the invasive capacity of the freshwater bivalve, Anodonta woodiana (Bivalvia: Unionacea): a comparative study with the indigenous species of the genus, Anodonta sp.
    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (p

  • A potential role of cholinesterases in the invasive capacity of the freshwater bivalve Anodonta woodiana (Lea, 1834) (Bivalvia: unionacea): a comparative study with the indigenous species of the genus Anodonta sp. (Linnaeus, 1758).
    2007
    Co-Authors: Ilaria Corsi, Angela Maria Pastore, Antonella Lodde, Lucio Castagnolo, Emanuela Palmerini, Silvano Focardi
    Abstract:

    To address the potential role of cholinesterase enzymes in the invasive capacity of species, the present study investigated ChE activity in the invasive freshwater bivalve Anodonta woodiana (Lea, 1834) comparing it with that of the indigenous species, Anodonta sp. (Linnaeus, 1758). The invasive capacity of pests has often been linked to their ecological plasticity and high intrinsic genetic variability; however the role played by molecular and cellular mechanisms, generally known as an organism's response to pollution, is unclear. Different substrates and selective ChE enzyme inhibitors were investigated in digestive gland, foot, gonad, adductor muscle and gill tissues while sensitivity to four organophosphate (OP) insecticides was investigated in vitro only in adductor muscle. The invasive species (A. woodiana) showed significantly greater (at least one order of magnitude) ChE activity than the endemic species (Anodonta sp.) (pb0.05) using acetylthiocholine (ASCh) as substrate and the activity was more widely distributed in tissues involved in movement (adductor muscle and foot), respiration, feeding (gills) and reproduction (gonads). Moreover, only the invasive species, A. woodiana, showed detectable ChE (vs. ASCh) activity in gill tissue. No substrate specificity was observed in any tissue of either species as already described for other bivalve species. ChE activity was not inhibited by Iso-OMPA but showed high sensitivity to BW248c51 and eserine. Both species showed moderate to low sensitivities in vitro to OP insecticides in the range 10−7–10−2 M. Calculated IC50 for fenitrothion and chlorpyrifos was in the range 10−6–10−3 M in muscle of A. woodiana while a higher inhibition was observed for fenitrothion (10−7 M) and lower for chlorpyrifos (10−2 M) in the indigenous species Anodonta sp. Similar IC50 of 10−5–10−6 M were observed for DFP and azamethiphos in both species. The hypotheses of other authors that acetylcholinesterase (AChE) is involved in the control of many essential functions, such as frontal ciliary activity of gill epithelium, temperature resistance, ciliary activity for transport of suspended particulate, valve opening and embryo development, suggest that the high catalytic efficiency of the invasive species may endow it with a competitive advantage over the endemic species. In view of the peculiar reproductive strategy of these mussels, higher ChE vs. ASCh activity in gonads of the invasive species could also favour glochidium production and embryo development under a wider range of environmental conditions