The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Michaela Drogemuller - One of the best experts on this subject based on the ideXlab platform.
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erratum to amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods vet parasitol 124 2004 205 215
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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Erratum to “Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods” [Vet. Parasitol. 124 (2004) 205–215]
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von Samson-himmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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Erratum to “Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods” [Vet. Parasitol. 124 (2004) 205–215]
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von Samson-himmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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erratum to amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods vet parasitol 124 2004 205 215
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods
Veterinary Parasitology, 2004Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Abstract The diagnosis of tapeworm infections in horses relies on copro-diagnostic methods, which are time-consuming and of limited sensitivity for determination of the exact prevalence. The development of serological tests has slightly improved the detection of tapeworm infections, but more sensitive methods are still required. A polymerase chain reaction (PCR)-based approach may constitute a valuable tool to improve tapeworm diagnosis. Nuclear ribosomal DNA (rDNA) is a useful target for species and/or strain markers. Partial 18S, the internal transcribed spacer 1 (ITS-1), the 5.8S, the internal transcribed spacer 2 (ITS-2), and partial 28S rDNA of the equine tapeworms Anoplocephala perfoliata and Anoplocephaloides mamillana were amplified and sequenced. The lengths and GC contents of the regions sequenced were 2087–2091 bp and 49.35–49.69% for A. perfoliata, and 2110–2119 bp and 49.15–49.32% for A. mamillana, respectively. Sequence alignment and comparison of both taxa showed 79.3–80.2% identity. The lowest identities were found in the ITS regions with 39.9–43.5% for the ITS-1 and 59.5–61.2% for the ITS-2. No matches of the ITS-2 of A. perfoliata and A. mamillana were found with other species by BLAST search. For this reason, ITS-2 sequences seemed appropriate as accurate species markers and A. perfoliata ITS-2 primers were developed. The ITS-2 PCR enabled the detection of genomic DNA as low as 0.5 pgs. First efforts on the practical application of the PCR-based approach were made. A 6-mg fragment of a tapeworm proglottid was detected in 0.5 and 1 g of faeces.
A. Uggla - One of the best experts on this subject based on the ideXlab platform.
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Epidemiology of Anoplocephala perfoliata infection in foals on a stud farm in south-western Sweden.
Veterinary parasitology, 1998Co-Authors: J. Höglund, B.-l. Ljungström, O. Nilsson, Johan Hellander, Eva Osterman Lind, A. UgglaAbstract:The egg output and humoral antibody response to scolex antigens of the equine tapeworm Anoplocephala perfoliata were monitored in naturally infected foals by an egg flotation/centrifugation method and an indirect enzyme-linked immunosorbent assay (ELISA). The study was performed on a stud farm in south-western Sweden between May 1994 and April 1995. Sequential blood and faecal samples were taken from 21 foals during their first summer on pasture and until tapeworm eggs were detected. Results were expressed separately for 10 and 11 foals born before and after the end of April 1994, respectively. Increased levels of antibodies were noticed from October and onwards in both groups whereas tapeworm eggs were detected in the faeces of all foals about 4 months later. The antibody response was similar in both groups but it was more pronounced in foals born before April 1994. All foals were treated in March 1995 with an oral paste formulation of pyrantel pamoate at a dose rate of 38 mg/kg bodyweight. Most animals responded to the anthelmintic treatment and one month later, tapeworm eggs were only detected in one out of the 18 foals examined one month after treatment. Thus, the treatment reduced the number of A. perfoliata egg positive horses by 94%. A concomitant decline in antibody levels was also observed. Western immunoblot analysis of sequential individual serum samples showed that at least 10 different scolex antigens in the molecular weight range 10-200 kDa were recognised. Banding intensities, especially of the 10 kDa, 45 kDa and 66 kDa proteins were different in the sequential sera taken during the course of infection.
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Anoplocephala perfoliata in Horses in Sweden: Prevalence, Infection Levels and Intestinal Lesions
Acta Veterinaria Scandinavica, 1995Co-Authors: O. Nilsson, B.-l. Ljungström, J. Höglund, H. Lundquist, A. UgglaAbstract:Distal ileum, caecum and proximal colon of 470 horses were examined for helminths during 1 year at an abattoir in central Sweden. The infection levels of the horse tapeworm Anoplocephala perfoliata, their stage of development, site of attachment and gross pathological lesions caused by the worm were recorded. Faecal samples from 395 of the horses were examined specifically for tapeworm segments and eggs in order to correlate these findings with the numbers in the alimentary canal. In total 65% of the horses were infected with A. perfoliata and the mean intensity of infection was 79 worms per infected horse with a maximum of 912. The level of infection was significantly higher in (1) 3rd and 4th than in 1st and 2nd quarter of the year; (2) older horses than in yearlings; (3) females than in males and geldings; (4) thoroughbreed and cold-blooded horses than in Swedish standardbreeds and ponies. The level of infection was unaffected by the usage of anthelminthics against nematodes. Of the horses examined 51% had 1-100 worms whereas 14% were infected with more than 100 worms. Of the tapeworm positive horses 72% had mixed infections with both adult and juvenile worms, 20% solely juveniles, and 8% solely adults. The severity of intestinal lesions exacerbated by increasing numbers of A. perfoliata. About 11% of the intestines examined had severe lesions, but there was no history of acute abdominal distress in any of the horses included in this study. Although the number of detectable eggs was significantly higher for horses heavily infected with A. perfoliata, the egg recovery among infected horses was only 35%. An additional field survey comprising 218 horses on 88 premises in central and southern parts of Sweden showed that the prevalence of A. perfoliata egg positive horses was the same as found on faecal examination during the abattoir survey.
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Anoplocephala perfoliata in horses in Sweden: prevalence, infection levels and intestinal lesions.
Acta veterinaria Scandinavica, 1995Co-Authors: O. Nilsson, B.-l. Ljungström, J. Höglund, Lundquist H, A. UgglaAbstract:Distal ileum, caecum and proximal colon of 470 horses were examined for helminths during 1 year at an abattoir in central Sweden. The infection levels of the horse tapeworm Anoplocephala perfoliata, their stage of development, site of attachment and gross pathological lesions caused by the worm were recorded. Faecal samples from 395 of the horses were examined specifically for tapeworm segments and eggs in order to correlate these findings with the numbers in the alimentary canal. In total 65% of the horses were infected with A. perfoliata and the mean intensity of infection was 79 worms per infected horse with a maximum of 912. The level of infection was significantly higher in (1) 3rd and 4th than in 1st and 2nd quarter of the year; (2) older horses than in yearlings; (3) females than in males and geldings; (4) thoroughbred and cold-blooded horses than in Swedish standard breeds and ponies. The level of infection was unaffected by the usage of anthelminthics against nematodes. Of the horses examined 51% had 1-100 worms whereas 14% were infected with more than 100 worms. Of the tapeworm positive horses 72% had mixed infections with both adult and juvenile worms, 20% solely juveniles, and 8% solely adults. The severity of intestinal lesions exacerbated by increasing numbers of A. perfoliata. About 11% of the intestines examined had severe lesions, but there was no history of acute abdominal distress in any of the horses included in this study. Although the number of detectable eggs was significantly higher for horses heavily infected with A. perfoliata, the egg recovery among infected horses was only 35%. An additional field survey comprising 218 horses on 88 premises in central and southern parts of Sweden showed that the prevalence of A. perfoliata egg positive horses was the same as found on faecal examination during the abattoir survey.
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Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Anoplocephala perfoliata in horse sera.
Veterinary parasitology, 1995Co-Authors: J. Höglund, B.-l. Ljungström, O. Nilsson, A. UgglaAbstract:A scolex antigen of the horse tapeworm Anoplocephala perfoliata containing at least 14 different proteins was employed in an enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to A. perfoliata in equine sera. The assay was applied to sera from 426 slaughtered horses with different numbers of worms and with varying degrees of intestinal lesions. As measured by the ELISA, there was a very strong effect on the antibody levels both from the number of tapeworms present and from the intestinal lesion score. However, considerable individual variation was observed between horses with similar worm counts. The ELISA values of horses that were either negative for strongyles and tapeworms or positive only for strongyles were similar, whereas both of these groups differed significantly from animals that harboured only A. perfoliata. Thus it seemed that cross-reactivity with concurrent nematode infections did not occur. Furthermore, a significant seasonal pattern in antibody levels was observed which reflected the establishment of newly acquired infections. No observations were made of confounding effects owing to age and previous anthelmintic treatment of the horses. It is concluded that the ELISA presented here has a potential for monitoring A. perfoliata infection on a herd level and can be used as a complementary diagnostic tool in epidemiological studies. The present study indicates that transmission ofA. perfoliata in Central Sweden occurred during a short period in spring and more consistently in late autumn.
Georg Von Samsonhimmelstjerna - One of the best experts on this subject based on the ideXlab platform.
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erratum to amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods vet parasitol 124 2004 205 215
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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erratum to amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods vet parasitol 124 2004 205 215
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods
Veterinary Parasitology, 2004Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Abstract The diagnosis of tapeworm infections in horses relies on copro-diagnostic methods, which are time-consuming and of limited sensitivity for determination of the exact prevalence. The development of serological tests has slightly improved the detection of tapeworm infections, but more sensitive methods are still required. A polymerase chain reaction (PCR)-based approach may constitute a valuable tool to improve tapeworm diagnosis. Nuclear ribosomal DNA (rDNA) is a useful target for species and/or strain markers. Partial 18S, the internal transcribed spacer 1 (ITS-1), the 5.8S, the internal transcribed spacer 2 (ITS-2), and partial 28S rDNA of the equine tapeworms Anoplocephala perfoliata and Anoplocephaloides mamillana were amplified and sequenced. The lengths and GC contents of the regions sequenced were 2087–2091 bp and 49.35–49.69% for A. perfoliata, and 2110–2119 bp and 49.15–49.32% for A. mamillana, respectively. Sequence alignment and comparison of both taxa showed 79.3–80.2% identity. The lowest identities were found in the ITS regions with 39.9–43.5% for the ITS-1 and 59.5–61.2% for the ITS-2. No matches of the ITS-2 of A. perfoliata and A. mamillana were found with other species by BLAST search. For this reason, ITS-2 sequences seemed appropriate as accurate species markers and A. perfoliata ITS-2 primers were developed. The ITS-2 PCR enabled the detection of genomic DNA as low as 0.5 pgs. First efforts on the practical application of the PCR-based approach were made. A 6-mg fragment of a tapeworm proglottid was detected in 0.5 and 1 g of faeces.
Kurt Pfister - One of the best experts on this subject based on the ideXlab platform.
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erratum to amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods vet parasitol 124 2004 205 215
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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Erratum to “Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods” [Vet. Parasitol. 124 (2004) 205–215]
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von Samson-himmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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Erratum to “Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods” [Vet. Parasitol. 124 (2004) 205–215]
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von Samson-himmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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erratum to amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods vet parasitol 124 2004 205 215
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods
Veterinary Parasitology, 2004Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Abstract The diagnosis of tapeworm infections in horses relies on copro-diagnostic methods, which are time-consuming and of limited sensitivity for determination of the exact prevalence. The development of serological tests has slightly improved the detection of tapeworm infections, but more sensitive methods are still required. A polymerase chain reaction (PCR)-based approach may constitute a valuable tool to improve tapeworm diagnosis. Nuclear ribosomal DNA (rDNA) is a useful target for species and/or strain markers. Partial 18S, the internal transcribed spacer 1 (ITS-1), the 5.8S, the internal transcribed spacer 2 (ITS-2), and partial 28S rDNA of the equine tapeworms Anoplocephala perfoliata and Anoplocephaloides mamillana were amplified and sequenced. The lengths and GC contents of the regions sequenced were 2087–2091 bp and 49.35–49.69% for A. perfoliata, and 2110–2119 bp and 49.15–49.32% for A. mamillana, respectively. Sequence alignment and comparison of both taxa showed 79.3–80.2% identity. The lowest identities were found in the ITS regions with 39.9–43.5% for the ITS-1 and 59.5–61.2% for the ITS-2. No matches of the ITS-2 of A. perfoliata and A. mamillana were found with other species by BLAST search. For this reason, ITS-2 sequences seemed appropriate as accurate species markers and A. perfoliata ITS-2 primers were developed. The ITS-2 PCR enabled the detection of genomic DNA as low as 0.5 pgs. First efforts on the practical application of the PCR-based approach were made. A 6-mg fragment of a tapeworm proglottid was detected in 0.5 and 1 g of faeces.
Pamela Beelitz - One of the best experts on this subject based on the ideXlab platform.
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erratum to amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods vet parasitol 124 2004 205 215
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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Erratum to “Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods” [Vet. Parasitol. 124 (2004) 205–215]
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von Samson-himmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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Erratum to “Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods” [Vet. Parasitol. 124 (2004) 205–215]
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von Samson-himmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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erratum to amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods vet parasitol 124 2004 205 215
Veterinary Parasitology, 2005Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Erratum to ‘‘Amplification of ribosomal DNA of Anoplocephalidae: Anoplocephala perfoliata diagnosis by PCR as a possible alternative to coprological methods’’ [Vet. Parasitol. 124 (2004) 205–215] Michaela Drogemuller , Pamela Beelitz , Kurt Pfister , Thomas Schnieder , Georg von Samson-Himmelstjerna a,* a Institute of Parasitology, School of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany b Faculty of Veterinary Medicine, Institute of Comparative Tropical Medicine and Parasitology, Ludwig Maximilian University, Veterinarstr. 13, D-80539 Munich, Germany
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amplification of ribosomal dna of anoplocephalidae Anoplocephala perfoliata diagnosis by pcr as a possible alternative to coprological methods
Veterinary Parasitology, 2004Co-Authors: Michaela Drogemuller, Pamela Beelitz, Kurt Pfister, Thomas Schnieder, Georg Von SamsonhimmelstjernaAbstract:Abstract The diagnosis of tapeworm infections in horses relies on copro-diagnostic methods, which are time-consuming and of limited sensitivity for determination of the exact prevalence. The development of serological tests has slightly improved the detection of tapeworm infections, but more sensitive methods are still required. A polymerase chain reaction (PCR)-based approach may constitute a valuable tool to improve tapeworm diagnosis. Nuclear ribosomal DNA (rDNA) is a useful target for species and/or strain markers. Partial 18S, the internal transcribed spacer 1 (ITS-1), the 5.8S, the internal transcribed spacer 2 (ITS-2), and partial 28S rDNA of the equine tapeworms Anoplocephala perfoliata and Anoplocephaloides mamillana were amplified and sequenced. The lengths and GC contents of the regions sequenced were 2087–2091 bp and 49.35–49.69% for A. perfoliata, and 2110–2119 bp and 49.15–49.32% for A. mamillana, respectively. Sequence alignment and comparison of both taxa showed 79.3–80.2% identity. The lowest identities were found in the ITS regions with 39.9–43.5% for the ITS-1 and 59.5–61.2% for the ITS-2. No matches of the ITS-2 of A. perfoliata and A. mamillana were found with other species by BLAST search. For this reason, ITS-2 sequences seemed appropriate as accurate species markers and A. perfoliata ITS-2 primers were developed. The ITS-2 PCR enabled the detection of genomic DNA as low as 0.5 pgs. First efforts on the practical application of the PCR-based approach were made. A 6-mg fragment of a tapeworm proglottid was detected in 0.5 and 1 g of faeces.