The Experts below are selected from a list of 1083 Experts worldwide ranked by ideXlab platform

Brian R Roome - One of the best experts on this subject based on the ideXlab platform.

  • netrin1 and reelin signaling are required for the migration of Anterolateral System neurons in the embryonic spinal cord
    Pain, 2021
    Co-Authors: Brian R Roome, Shima Rastegarpouyani, Annie Dumouchel, Amy Ker, Marie Kmita, Artur Kania
    Abstract:

    ABSTRACT Projection neurons of the spinal cord dorsal horn which transmit pain, itch and temperature information to the brain comprise the Anterolateral System (AS). A recent molecular and genetic study showed that many developing AS neurons express the transcription factor Phox2a, and provided insights into the mechanisms of their ontogeny and wiring of nociceptive neuronal circuits. Here we show that the loss of the axonal guidance and neuronal migration signal netrin1 results in impaired migration of mouse Phox2a+ AS neurons into the spinal lamina I. Furthermore, we show that in the absence of Dab1, an intracellular transducer of the neuronal migration signal reelin, the migration of spinal lamina V and lateral spinal nucleus Phox2a+ AS neurons is impaired, in line with deficits in nociception seen in mice with a loss of reelin signaling. Together, these results provide evidence that netrin1 and reelin control the development of spinal nociceptive projection neurons, suggesting a mechanistic explanation for studies that link sequence variations in human genes encoding these neurodevelopmental signals and abnormal pain sensation.

  • phox2a defines a developmental origin of the Anterolateral System in mice and humans
    Cell Reports, 2020
    Co-Authors: Brian R Roome, Farin B Bourojeni, Bishakha Mona, Shima Rastegarpouyani, Raphael Blain, Annie Dumouchel, Charleen Salesse, Scott W Thompson, Megan Brookbank, Yorick Gitton
    Abstract:

    Anterolateral System neurons relay pain, itch, and temperature information from the spinal cord to pain-related brain regions, but the differentiation of these neurons and their specific contribution to pain perception remain poorly defined. Here, we show that most mouse spinal neurons that embryonically express the autonomic-System-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive brain targets, including the parabrachial nucleus and the thalamus. We define the Phox2a Anterolateral System neuron birth order, migration, and differentiation and uncover an essential role for Phox2a in the development of relay of nociceptive signals from the spinal cord to the brain. Finally, we also demonstrate that the molecular identity of Phox2a neurons is conserved in the human fetal spinal cord, arguing that the developmental expression of Phox2a is a prominent feature of Anterolateral System neurons.

  • phox2a defines a developmental origin of the Anterolateral System in mice and humans
    bioRxiv, 2020
    Co-Authors: Brian R Roome, Farin B Bourojeni, Bishakha Mona, Shima Rastegarpouyani, Raphael Blain, Annie Dumouchel, Charleen Salesse
    Abstract:

    Summary Anterolateral System neurons relay pain, itch and temperature information from the spinal cord to pain-related brain regions, but the differentiation of these neurons and their specific contribution to pain perception remain poorly defined. Here, we show that virtually all mouse spinal neurons that embryonically express the autonomic System-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive brain targets, including the parabrachial nucleus and the thalamus. We define Phox2a Anterolateral System neuron birth order, migration and differentiation, and uncover an essential role for Phox2a in the development of relay of nociceptive signals from the spinal cord to the brain. Finally, we also demonstrate that the molecular identity of Phox2a neurons is conserved in the human foetal spinal cord. The developmental expression of Phox2a as a uniting feature of Anterolateral System neurons suggests a link between nociception and autonomic nervous System function.

Farin B Bourojeni - One of the best experts on this subject based on the ideXlab platform.

  • netrin 1 receptor dcc is required for the contralateral topography of lamina i Anterolateral System neurons
    Pain, 2021
    Co-Authors: Farin B Bourojeni, Hanns Ulrich Zeilhofer, Artur Kania
    Abstract:

    Anterolateral System (AS) neurons relay nociceptive information from the spinal cord to the brain, protecting the body from harm by evoking a variety of behaviours and autonomic responses. The developmental programs that guide the connectivity of AS neurons remain poorly understood. Spinofugal axons cross the spinal midline in response to Netrin-1 signalling through its receptor deleted in colorectal carcinoma (DCC); however, the relevance of this canonical pathway to AS neuron development has only been demonstrated recently. Here, we disrupted Netrin-1:DCC signalling developmentally in AS neurons and assessed the consequences on the path finding of the different classes of spinofugal neurons. Many lamina I AS neurons normally innervate the lateral parabrachial nucleus and periaqueductal gray on the contralateral side. The loss of DCC in the developing spinal cord resulted in increased frequency of ipsilateral projection of spinoparabrachial and spinoperiaqueductal gray neurons. Given that contralateral spinofugal projections are largely associated with somatotopic representation of the body, changes in the laterality of AS spinofugal projections may contribute to reduced precision in pain localization observed in mice and humans carrying Dcc mutations.

  • phox2a defines a developmental origin of the Anterolateral System in mice and humans
    Cell Reports, 2020
    Co-Authors: Brian R Roome, Farin B Bourojeni, Bishakha Mona, Shima Rastegarpouyani, Raphael Blain, Annie Dumouchel, Charleen Salesse, Scott W Thompson, Megan Brookbank, Yorick Gitton
    Abstract:

    Anterolateral System neurons relay pain, itch, and temperature information from the spinal cord to pain-related brain regions, but the differentiation of these neurons and their specific contribution to pain perception remain poorly defined. Here, we show that most mouse spinal neurons that embryonically express the autonomic-System-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive brain targets, including the parabrachial nucleus and the thalamus. We define the Phox2a Anterolateral System neuron birth order, migration, and differentiation and uncover an essential role for Phox2a in the development of relay of nociceptive signals from the spinal cord to the brain. Finally, we also demonstrate that the molecular identity of Phox2a neurons is conserved in the human fetal spinal cord, arguing that the developmental expression of Phox2a is a prominent feature of Anterolateral System neurons.

  • phox2a defines a developmental origin of the Anterolateral System in mice and humans
    bioRxiv, 2020
    Co-Authors: Brian R Roome, Farin B Bourojeni, Bishakha Mona, Shima Rastegarpouyani, Raphael Blain, Annie Dumouchel, Charleen Salesse
    Abstract:

    Summary Anterolateral System neurons relay pain, itch and temperature information from the spinal cord to pain-related brain regions, but the differentiation of these neurons and their specific contribution to pain perception remain poorly defined. Here, we show that virtually all mouse spinal neurons that embryonically express the autonomic System-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive brain targets, including the parabrachial nucleus and the thalamus. We define Phox2a Anterolateral System neuron birth order, migration and differentiation, and uncover an essential role for Phox2a in the development of relay of nociceptive signals from the spinal cord to the brain. Finally, we also demonstrate that the molecular identity of Phox2a neurons is conserved in the human foetal spinal cord. The developmental expression of Phox2a as a uniting feature of Anterolateral System neurons suggests a link between nociception and autonomic nervous System function.

Shima Rastegarpouyani - One of the best experts on this subject based on the ideXlab platform.

  • netrin1 and reelin signaling are required for the migration of Anterolateral System neurons in the embryonic spinal cord
    Pain, 2021
    Co-Authors: Brian R Roome, Shima Rastegarpouyani, Annie Dumouchel, Amy Ker, Marie Kmita, Artur Kania
    Abstract:

    ABSTRACT Projection neurons of the spinal cord dorsal horn which transmit pain, itch and temperature information to the brain comprise the Anterolateral System (AS). A recent molecular and genetic study showed that many developing AS neurons express the transcription factor Phox2a, and provided insights into the mechanisms of their ontogeny and wiring of nociceptive neuronal circuits. Here we show that the loss of the axonal guidance and neuronal migration signal netrin1 results in impaired migration of mouse Phox2a+ AS neurons into the spinal lamina I. Furthermore, we show that in the absence of Dab1, an intracellular transducer of the neuronal migration signal reelin, the migration of spinal lamina V and lateral spinal nucleus Phox2a+ AS neurons is impaired, in line with deficits in nociception seen in mice with a loss of reelin signaling. Together, these results provide evidence that netrin1 and reelin control the development of spinal nociceptive projection neurons, suggesting a mechanistic explanation for studies that link sequence variations in human genes encoding these neurodevelopmental signals and abnormal pain sensation.

  • phox2a defines a developmental origin of the Anterolateral System in mice and humans
    Cell Reports, 2020
    Co-Authors: Brian R Roome, Farin B Bourojeni, Bishakha Mona, Shima Rastegarpouyani, Raphael Blain, Annie Dumouchel, Charleen Salesse, Scott W Thompson, Megan Brookbank, Yorick Gitton
    Abstract:

    Anterolateral System neurons relay pain, itch, and temperature information from the spinal cord to pain-related brain regions, but the differentiation of these neurons and their specific contribution to pain perception remain poorly defined. Here, we show that most mouse spinal neurons that embryonically express the autonomic-System-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive brain targets, including the parabrachial nucleus and the thalamus. We define the Phox2a Anterolateral System neuron birth order, migration, and differentiation and uncover an essential role for Phox2a in the development of relay of nociceptive signals from the spinal cord to the brain. Finally, we also demonstrate that the molecular identity of Phox2a neurons is conserved in the human fetal spinal cord, arguing that the developmental expression of Phox2a is a prominent feature of Anterolateral System neurons.

  • phox2a defines a developmental origin of the Anterolateral System in mice and humans
    bioRxiv, 2020
    Co-Authors: Brian R Roome, Farin B Bourojeni, Bishakha Mona, Shima Rastegarpouyani, Raphael Blain, Annie Dumouchel, Charleen Salesse
    Abstract:

    Summary Anterolateral System neurons relay pain, itch and temperature information from the spinal cord to pain-related brain regions, but the differentiation of these neurons and their specific contribution to pain perception remain poorly defined. Here, we show that virtually all mouse spinal neurons that embryonically express the autonomic System-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive brain targets, including the parabrachial nucleus and the thalamus. We define Phox2a Anterolateral System neuron birth order, migration and differentiation, and uncover an essential role for Phox2a in the development of relay of nociceptive signals from the spinal cord to the brain. Finally, we also demonstrate that the molecular identity of Phox2a neurons is conserved in the human foetal spinal cord. The developmental expression of Phox2a as a uniting feature of Anterolateral System neurons suggests a link between nociception and autonomic nervous System function.

Annie Dumouchel - One of the best experts on this subject based on the ideXlab platform.

  • netrin1 and reelin signaling are required for the migration of Anterolateral System neurons in the embryonic spinal cord
    Pain, 2021
    Co-Authors: Brian R Roome, Shima Rastegarpouyani, Annie Dumouchel, Amy Ker, Marie Kmita, Artur Kania
    Abstract:

    ABSTRACT Projection neurons of the spinal cord dorsal horn which transmit pain, itch and temperature information to the brain comprise the Anterolateral System (AS). A recent molecular and genetic study showed that many developing AS neurons express the transcription factor Phox2a, and provided insights into the mechanisms of their ontogeny and wiring of nociceptive neuronal circuits. Here we show that the loss of the axonal guidance and neuronal migration signal netrin1 results in impaired migration of mouse Phox2a+ AS neurons into the spinal lamina I. Furthermore, we show that in the absence of Dab1, an intracellular transducer of the neuronal migration signal reelin, the migration of spinal lamina V and lateral spinal nucleus Phox2a+ AS neurons is impaired, in line with deficits in nociception seen in mice with a loss of reelin signaling. Together, these results provide evidence that netrin1 and reelin control the development of spinal nociceptive projection neurons, suggesting a mechanistic explanation for studies that link sequence variations in human genes encoding these neurodevelopmental signals and abnormal pain sensation.

  • phox2a defines a developmental origin of the Anterolateral System in mice and humans
    Cell Reports, 2020
    Co-Authors: Brian R Roome, Farin B Bourojeni, Bishakha Mona, Shima Rastegarpouyani, Raphael Blain, Annie Dumouchel, Charleen Salesse, Scott W Thompson, Megan Brookbank, Yorick Gitton
    Abstract:

    Anterolateral System neurons relay pain, itch, and temperature information from the spinal cord to pain-related brain regions, but the differentiation of these neurons and their specific contribution to pain perception remain poorly defined. Here, we show that most mouse spinal neurons that embryonically express the autonomic-System-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive brain targets, including the parabrachial nucleus and the thalamus. We define the Phox2a Anterolateral System neuron birth order, migration, and differentiation and uncover an essential role for Phox2a in the development of relay of nociceptive signals from the spinal cord to the brain. Finally, we also demonstrate that the molecular identity of Phox2a neurons is conserved in the human fetal spinal cord, arguing that the developmental expression of Phox2a is a prominent feature of Anterolateral System neurons.

  • phox2a defines a developmental origin of the Anterolateral System in mice and humans
    bioRxiv, 2020
    Co-Authors: Brian R Roome, Farin B Bourojeni, Bishakha Mona, Shima Rastegarpouyani, Raphael Blain, Annie Dumouchel, Charleen Salesse
    Abstract:

    Summary Anterolateral System neurons relay pain, itch and temperature information from the spinal cord to pain-related brain regions, but the differentiation of these neurons and their specific contribution to pain perception remain poorly defined. Here, we show that virtually all mouse spinal neurons that embryonically express the autonomic System-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive brain targets, including the parabrachial nucleus and the thalamus. We define Phox2a Anterolateral System neuron birth order, migration and differentiation, and uncover an essential role for Phox2a in the development of relay of nociceptive signals from the spinal cord to the brain. Finally, we also demonstrate that the molecular identity of Phox2a neurons is conserved in the human foetal spinal cord. The developmental expression of Phox2a as a uniting feature of Anterolateral System neurons suggests a link between nociception and autonomic nervous System function.

Yorick Gitton - One of the best experts on this subject based on the ideXlab platform.

  • phox2a defines a developmental origin of the Anterolateral System in mice and humans
    Cell Reports, 2020
    Co-Authors: Brian R Roome, Farin B Bourojeni, Bishakha Mona, Shima Rastegarpouyani, Raphael Blain, Annie Dumouchel, Charleen Salesse, Scott W Thompson, Megan Brookbank, Yorick Gitton
    Abstract:

    Anterolateral System neurons relay pain, itch, and temperature information from the spinal cord to pain-related brain regions, but the differentiation of these neurons and their specific contribution to pain perception remain poorly defined. Here, we show that most mouse spinal neurons that embryonically express the autonomic-System-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive brain targets, including the parabrachial nucleus and the thalamus. We define the Phox2a Anterolateral System neuron birth order, migration, and differentiation and uncover an essential role for Phox2a in the development of relay of nociceptive signals from the spinal cord to the brain. Finally, we also demonstrate that the molecular identity of Phox2a neurons is conserved in the human fetal spinal cord, arguing that the developmental expression of Phox2a is a prominent feature of Anterolateral System neurons.