The Experts below are selected from a list of 42132 Experts worldwide ranked by ideXlab platform
Xinmiao Liang - One of the best experts on this subject based on the ideXlab platform.
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high performance liquid chromatography separation of cis trans anthocyanin isomers from wild lycium ruthenicum murr employing a mixed mode reversed phase strong anion exchange stationary phase
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Fan Yang, Jixia Wang, Xiaolong Li, Xiaojun Peng, Xinmiao LiangAbstract:The cis–trans isomerism is a common phenomenon for acylated Anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric Anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric Anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure Anthocyanins, including two pairs of cis–trans isomeric Anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the r...
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high performance liquid chromatography separation of cis trans anthocyanin isomers from wild lycium ruthenicum murr employing a mixed mode reversed phase strong anion exchange stationary phase
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Hongli Jin, Jixia Wang, Fan Yang, Xiaojun Peng, Yanfang Liu, Zhimour Guo, Xinmiao LiangAbstract:The cis-trans isomerism is a common phenomenon for acylated Anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric Anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric Anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure Anthocyanins, including two pairs of cis-trans isomeric Anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the results indicated that this method is an effective way to separate Anthocyanins, especially for cis-trans isomers.
Hongli Jin - One of the best experts on this subject based on the ideXlab platform.
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preparative separation of a challenging anthocyanin from lycium ruthenicum murr by two dimensional reversed phase liquid chromatography hydrophilic interaction chromatography
RSC Advances, 2015Co-Authors: Fan Yang, Hongli Jin, Jianqiang Zhao, Weijia Zhou, Aijin Shen, Yanfang Liu, Zhimou Guo, Xiuli Zhang, Yanduo TaoAbstract:The preparative separation of Anthocyanins by HPLC often suffers from insufficient separation selectivity. In this work, a two-dimensional liquid (LC-LC) method was established to efficiently purify a challenging anthocyanin in Lycium ruthenicum Murray. Reversed phase liquid chromatography (RPLC) was used in the first-dimension preparation to fractionate the sample for its high separation efficiency. After the optimization of second-dimension methods, hydrophilic interaction chromatography (HILIC) was applied to further isolate the anthocyanin for the good orthogonality to RPLC. To improve HILIC separation for Anthocyanins, stationary phases and mobile phases were investigated systematically. A satisfactory result was obtained on a zwitterionic Click XIon column with 1% phosphoric acid as an acidic additive. Using the above method, the anthocyanin and three new alkaloids were isolated from L. ruthenicum for the first time. This RPLC/HILIC method solved the coelution problem of anthocyanin and basic non-Anthocyanins in one-dimensional HPLC, benefiting from the significantly improved separation resolution.
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high performance liquid chromatography separation of cis trans anthocyanin isomers from wild lycium ruthenicum murr employing a mixed mode reversed phase strong anion exchange stationary phase
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Hongli Jin, Jixia Wang, Fan Yang, Xiaojun Peng, Yanfang Liu, Zhimour Guo, Xinmiao LiangAbstract:The cis-trans isomerism is a common phenomenon for acylated Anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric Anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric Anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure Anthocyanins, including two pairs of cis-trans isomeric Anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the results indicated that this method is an effective way to separate Anthocyanins, especially for cis-trans isomers.
Fan Yang - One of the best experts on this subject based on the ideXlab platform.
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preparative separation of a challenging anthocyanin from lycium ruthenicum murr by two dimensional reversed phase liquid chromatography hydrophilic interaction chromatography
RSC Advances, 2015Co-Authors: Fan Yang, Hongli Jin, Jianqiang Zhao, Weijia Zhou, Aijin Shen, Yanfang Liu, Zhimou Guo, Xiuli Zhang, Yanduo TaoAbstract:The preparative separation of Anthocyanins by HPLC often suffers from insufficient separation selectivity. In this work, a two-dimensional liquid (LC-LC) method was established to efficiently purify a challenging anthocyanin in Lycium ruthenicum Murray. Reversed phase liquid chromatography (RPLC) was used in the first-dimension preparation to fractionate the sample for its high separation efficiency. After the optimization of second-dimension methods, hydrophilic interaction chromatography (HILIC) was applied to further isolate the anthocyanin for the good orthogonality to RPLC. To improve HILIC separation for Anthocyanins, stationary phases and mobile phases were investigated systematically. A satisfactory result was obtained on a zwitterionic Click XIon column with 1% phosphoric acid as an acidic additive. Using the above method, the anthocyanin and three new alkaloids were isolated from L. ruthenicum for the first time. This RPLC/HILIC method solved the coelution problem of anthocyanin and basic non-Anthocyanins in one-dimensional HPLC, benefiting from the significantly improved separation resolution.
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high performance liquid chromatography separation of cis trans anthocyanin isomers from wild lycium ruthenicum murr employing a mixed mode reversed phase strong anion exchange stationary phase
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Fan Yang, Jixia Wang, Xiaolong Li, Xiaojun Peng, Xinmiao LiangAbstract:The cis–trans isomerism is a common phenomenon for acylated Anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric Anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric Anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure Anthocyanins, including two pairs of cis–trans isomeric Anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the r...
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high performance liquid chromatography separation of cis trans anthocyanin isomers from wild lycium ruthenicum murr employing a mixed mode reversed phase strong anion exchange stationary phase
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Hongli Jin, Jixia Wang, Fan Yang, Xiaojun Peng, Yanfang Liu, Zhimour Guo, Xinmiao LiangAbstract:The cis-trans isomerism is a common phenomenon for acylated Anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric Anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric Anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure Anthocyanins, including two pairs of cis-trans isomeric Anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the results indicated that this method is an effective way to separate Anthocyanins, especially for cis-trans isomers.
Xiaojun Peng - One of the best experts on this subject based on the ideXlab platform.
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high performance liquid chromatography separation of cis trans anthocyanin isomers from wild lycium ruthenicum murr employing a mixed mode reversed phase strong anion exchange stationary phase
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Fan Yang, Jixia Wang, Xiaolong Li, Xiaojun Peng, Xinmiao LiangAbstract:The cis–trans isomerism is a common phenomenon for acylated Anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric Anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric Anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure Anthocyanins, including two pairs of cis–trans isomeric Anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the r...
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high performance liquid chromatography separation of cis trans anthocyanin isomers from wild lycium ruthenicum murr employing a mixed mode reversed phase strong anion exchange stationary phase
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Hongli Jin, Jixia Wang, Fan Yang, Xiaojun Peng, Yanfang Liu, Zhimour Guo, Xinmiao LiangAbstract:The cis-trans isomerism is a common phenomenon for acylated Anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric Anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric Anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure Anthocyanins, including two pairs of cis-trans isomeric Anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the results indicated that this method is an effective way to separate Anthocyanins, especially for cis-trans isomers.
Jixia Wang - One of the best experts on this subject based on the ideXlab platform.
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high performance liquid chromatography separation of cis trans anthocyanin isomers from wild lycium ruthenicum murr employing a mixed mode reversed phase strong anion exchange stationary phase
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Fan Yang, Jixia Wang, Xiaolong Li, Xiaojun Peng, Xinmiao LiangAbstract:The cis–trans isomerism is a common phenomenon for acylated Anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric Anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric Anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure Anthocyanins, including two pairs of cis–trans isomeric Anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the r...
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high performance liquid chromatography separation of cis trans anthocyanin isomers from wild lycium ruthenicum murr employing a mixed mode reversed phase strong anion exchange stationary phase
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Hongli Jin, Jixia Wang, Fan Yang, Xiaojun Peng, Yanfang Liu, Zhimour Guo, Xinmiao LiangAbstract:The cis-trans isomerism is a common phenomenon for acylated Anthocyanins. Nevertheless, few studies reported effective methods for the preparation of isomeric Anthocyanins from natural products. In this work, a high-performance liquid chromatography (HPLC) method was developed to efficiently purify anthocyanin isomers from Lycium ruthenicum Murr. based on a mixed-mode reversed-phase/strong anion-exchange column (named XCharge C8SAX). Four commercially available columns were evaluated with a pair of isomeric Anthocyanins, and the results demonstrated that the XCharge C8SAX column exhibited improved selectivity and column efficiency for the isomers. The chromatographic parameters, including pH, organic content, and ionic strength, were investigated. Optimal separation quality for the anthocyanin isomers was achieved on the XCharge C8SAX column. Six pure Anthocyanins, including two pairs of cis-trans isomeric Anthocyanins with one new anthocyanin, were purified from L. ruthenicum and identified. All of the results indicated that this method is an effective way to separate Anthocyanins, especially for cis-trans isomers.