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Goverdina C H Derksen - One of the best experts on this subject based on the ideXlab platform.
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two validated hplc methods for the quantification of alizarin and other Anthraquinones in rubia tinctorum cultivars
Phytochemical Analysis, 2004Co-Authors: Goverdina C H Derksen, G P Lelyveld, Teris A Van Beek, Anthony Capelle, A E De GrootAbstract:Direct and indirect HPLC-UV methods for the quantitative determination of Anthraquinones in dried madder root have been developed, validated and compared. In the direct method, madder root was extracted twice with refluxing ethanol-water. This method allowed the determination of the two major native Anthraquinone Glycosides lucidin primeveroside and ruberythric acid. In the indirect extraction method, the Anthraquinone Glycosides were first converted into aglycones by endogenous enzymes and the aglycones were subsequently extracted with tetrahydroturan-water and then analysed. In this case the Anthraquinones alizarin, purpurin and nordamnacanthal may be determined. The content of nordamnacanthal is proportional to the amount of lucidin primeveroside 14 originally present. The indirect extraction method is easier to apply. Different madder cultivars were screened for their Anthraquinone content. Copyright (C) 2004 John Wiley Sons, Ltd.
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chemical and enzymatic hydrolysis of Anthraquinone Glycosides from madder roots
Phytochemical Analysis, 2003Co-Authors: Goverdina C H Derksen, M Naayer, T A Van Beek, A Capelle, I K Haaksman, H A Van Doren, A E De GrootAbstract:For the production of a commercially useful dye extract from madder, the glycoside ruberythric acid has to be hydrolysed to the aglycone alizarin which is the main dye component. An intrinsic problem is the simultaneous hydrolysis of the glycoside lucidin pritneveroside to the unwanted mutagenic aglycone lucidin. Madder root was treated with strong acid, strong base or enzymes to convert ruberythric acid into alizarin and the Anthraquinone compositions of the suspensions were analysed by HPLC. A cheap and easy method to hydrolyse ruberythric acid in madder root to alizarin without the formation of lucidin turned out to be the stirring of dried madder roots in water at room temperature for 90 min: this gave a suspension containing pseudopurpurin, munjistin, alizarin and nordamnacanthal. Native enzymes are responsible for the hydrolysis, after which lucidin is converted to nordamnacanthal by an endogenous oxidase. Copyright ©, 2003 John Wiley & Sons, Ltd.
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analysis of Anthraquinones in rubia tinctorum l by liquid chromatography coupled with diode array uv and mass spectrometric detection
Journal of Chromatography A, 2002Co-Authors: Goverdina C H Derksen, H A G Niederlander, Teris A Van BeekAbstract:A liquid chromatographic (LC) method for the separation of both Anthraquinone Glycosides and aglycones in extracts of Rubia tinctorum was improved. For on-line MS detection atmospheric pressure chemical ionisation as well as electrospray ionisation (ESI) were used. The Glycosides were ionised in both positive and negative ionisation (NI) mode, the aglycones only in the NI mode. With ESI ammonia was added to the eluent post-column to deprotonate the compounds. The efficiency of mass detection of the hydroxyAnthraquinone aglycones was found to depend on the pKa value of the component. LC–diode-array detection and LC–MS provide useful complementary information for the identification of Anthraquinones in plant extracts, which was proven with the identification of munjistin and pseudopurpurin.
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high performance liquid chromatographic method for the analysis of Anthraquinone Glycosides and aglycones in madder root rubia tinctorum l
Journal of Chromatography A, 1998Co-Authors: Goverdina C H Derksen, Teris A Van Beek, Aede De Groot, Anthony CapelleAbstract:A HPLC method has been developed for the simultaneous characterisation of Anthraquinone Glycosides and aglycones in extracts of Rubia tinctorum L. The Anthraquinones are separated on an end-capped C18-RP column with a water–acetonitrile gradient as eluent and measured with UV detection at 250 nm. With this method the Glycosides lucidin primeveroside and ruberythric acid and the aglycones lucidin, alizarin, purpurin, quinizarin and 2,6-dihydroxyAnthraquinone can be analysed. Lucidin which is not commercially available was synthesised starting from resorcinol and phthalic anhydride. The Glycosides ruberythric acid and lucidin primeveroside are commercially available as a mixture and were separated by droplet counter-current chromatography in ascending flow with chloroform–methanol–water as eluents prior to their use as standards.
Teris A Van Beek - One of the best experts on this subject based on the ideXlab platform.
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two validated hplc methods for the quantification of alizarin and other Anthraquinones in rubia tinctorum cultivars
Phytochemical Analysis, 2004Co-Authors: Goverdina C H Derksen, G P Lelyveld, Teris A Van Beek, Anthony Capelle, A E De GrootAbstract:Direct and indirect HPLC-UV methods for the quantitative determination of Anthraquinones in dried madder root have been developed, validated and compared. In the direct method, madder root was extracted twice with refluxing ethanol-water. This method allowed the determination of the two major native Anthraquinone Glycosides lucidin primeveroside and ruberythric acid. In the indirect extraction method, the Anthraquinone Glycosides were first converted into aglycones by endogenous enzymes and the aglycones were subsequently extracted with tetrahydroturan-water and then analysed. In this case the Anthraquinones alizarin, purpurin and nordamnacanthal may be determined. The content of nordamnacanthal is proportional to the amount of lucidin primeveroside 14 originally present. The indirect extraction method is easier to apply. Different madder cultivars were screened for their Anthraquinone content. Copyright (C) 2004 John Wiley Sons, Ltd.
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analysis of Anthraquinones in rubia tinctorum l by liquid chromatography coupled with diode array uv and mass spectrometric detection
Journal of Chromatography A, 2002Co-Authors: Goverdina C H Derksen, H A G Niederlander, Teris A Van BeekAbstract:A liquid chromatographic (LC) method for the separation of both Anthraquinone Glycosides and aglycones in extracts of Rubia tinctorum was improved. For on-line MS detection atmospheric pressure chemical ionisation as well as electrospray ionisation (ESI) were used. The Glycosides were ionised in both positive and negative ionisation (NI) mode, the aglycones only in the NI mode. With ESI ammonia was added to the eluent post-column to deprotonate the compounds. The efficiency of mass detection of the hydroxyAnthraquinone aglycones was found to depend on the pKa value of the component. LC–diode-array detection and LC–MS provide useful complementary information for the identification of Anthraquinones in plant extracts, which was proven with the identification of munjistin and pseudopurpurin.
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high performance liquid chromatographic method for the analysis of Anthraquinone Glycosides and aglycones in madder root rubia tinctorum l
Journal of Chromatography A, 1998Co-Authors: Goverdina C H Derksen, Teris A Van Beek, Aede De Groot, Anthony CapelleAbstract:A HPLC method has been developed for the simultaneous characterisation of Anthraquinone Glycosides and aglycones in extracts of Rubia tinctorum L. The Anthraquinones are separated on an end-capped C18-RP column with a water–acetonitrile gradient as eluent and measured with UV detection at 250 nm. With this method the Glycosides lucidin primeveroside and ruberythric acid and the aglycones lucidin, alizarin, purpurin, quinizarin and 2,6-dihydroxyAnthraquinone can be analysed. Lucidin which is not commercially available was synthesised starting from resorcinol and phthalic anhydride. The Glycosides ruberythric acid and lucidin primeveroside are commercially available as a mixture and were separated by droplet counter-current chromatography in ascending flow with chloroform–methanol–water as eluents prior to their use as standards.
Anthony Capelle - One of the best experts on this subject based on the ideXlab platform.
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two validated hplc methods for the quantification of alizarin and other Anthraquinones in rubia tinctorum cultivars
Phytochemical Analysis, 2004Co-Authors: Goverdina C H Derksen, G P Lelyveld, Teris A Van Beek, Anthony Capelle, A E De GrootAbstract:Direct and indirect HPLC-UV methods for the quantitative determination of Anthraquinones in dried madder root have been developed, validated and compared. In the direct method, madder root was extracted twice with refluxing ethanol-water. This method allowed the determination of the two major native Anthraquinone Glycosides lucidin primeveroside and ruberythric acid. In the indirect extraction method, the Anthraquinone Glycosides were first converted into aglycones by endogenous enzymes and the aglycones were subsequently extracted with tetrahydroturan-water and then analysed. In this case the Anthraquinones alizarin, purpurin and nordamnacanthal may be determined. The content of nordamnacanthal is proportional to the amount of lucidin primeveroside 14 originally present. The indirect extraction method is easier to apply. Different madder cultivars were screened for their Anthraquinone content. Copyright (C) 2004 John Wiley Sons, Ltd.
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high performance liquid chromatographic method for the analysis of Anthraquinone Glycosides and aglycones in madder root rubia tinctorum l
Journal of Chromatography A, 1998Co-Authors: Goverdina C H Derksen, Teris A Van Beek, Aede De Groot, Anthony CapelleAbstract:A HPLC method has been developed for the simultaneous characterisation of Anthraquinone Glycosides and aglycones in extracts of Rubia tinctorum L. The Anthraquinones are separated on an end-capped C18-RP column with a water–acetonitrile gradient as eluent and measured with UV detection at 250 nm. With this method the Glycosides lucidin primeveroside and ruberythric acid and the aglycones lucidin, alizarin, purpurin, quinizarin and 2,6-dihydroxyAnthraquinone can be analysed. Lucidin which is not commercially available was synthesised starting from resorcinol and phthalic anhydride. The Glycosides ruberythric acid and lucidin primeveroside are commercially available as a mixture and were separated by droplet counter-current chromatography in ascending flow with chloroform–methanol–water as eluents prior to their use as standards.
Guoying Zhou - One of the best experts on this subject based on the ideXlab platform.
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separation of three Anthraquinone Glycosides including two isomers by preparative high performance liquid chromatography and high speed countercurrent chromatography from rheum tanguticum maxim ex balf
IEEE Journal of Solid-state Circuits, 2016Co-Authors: Tao Chen, Chen Chen, Denglang Zou, Yongling Liu, Guoying ZhouAbstract:Anthraquinone Glycosides, such as chrysophanol 1-O-β-d-glucoside, chrysophanol 8-O-β-d-glucoside and physion 8-O-β-d-glucoside, are the accepted important active components of Rheum tanguticum Maxim. due to their pharmacological properties: antifungal, anti-microbial, cytotoxic and antioxidant activities. However, an effective method for the separation of the above Anthraquinone Glycosides from this herb is not currently available. Especially, greater difficulty existed in the separation of the two isomers chrysophanol 1-O-β-d-glucoside and chrysophanol 8-O-β-d-glucoside. This study demonstrated an efficient strategy based on preparative high-performance liquid chromatography and high-speed countercurrent chromatography for the separation of the above Anthraquinone Glycosides from Rheum tanguticum Maxim. This article is protected by copyright. All rights reserved
Peigen Xiao - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Open Access
2014Co-Authors: Zhe Wang, Yong Peng, Peigen XiaoAbstract:Background: Rhubarb is an important Chinese medicinal herb with a long history of over 2000 years and has been commonly used as a laxative. It is the radix and rhizome of Rheum officinale Baill., R. palmatum L. and R. tanguticum Maxim, all of which are mainly distributed in a broad region in the Tibetan plateau. Anthraquinone Glycosides are a series of major active ingredients found in all three species. They are key intermediates in the Anthraquinone secondary metabolism and the sennnoside biosynthesis. The variation of the Anthraquinone glycoside content in rhubarb in response to specific factors remains an attractive topic. Results: A simple and sensitive Ultra Performance Liquid Chromatography with Photo-Diode Array (UPLC-PDA) detector was developed for the simultaneous determination of six Anthraquinone Glycosides in rhubarb, i.e., aloeemodin-8-O-glucoside, rhein-8-O-glucoside, chrysophanol-1-O-glucoside, emodin-1-O-glucoside, chrysophanol-8-Oglucoside, emodin-8-O-glucoside. Twenty-seven batches from three species were submitted to the multi-component analysis. The results showed that the Anthraquinone glycoside content varied significantly even within the same species. The results showed that the Anthraquinone glycoside content varied significantly within the same species but not between different species. The PCA and content analysis results confirmed that the plant species has no obvious effect on the content variation. Neither was any significant correlation observed between the Anthraquinone glycosid
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evaluation of the content variation of Anthraquinone Glycosides in rhubarb by uplc pda
Chemistry Central Journal, 2013Co-Authors: Zhe Wang, Yong Peng, Peigen XiaoAbstract:Rhubarb is an important Chinese medicinal herb with a long history of over 2000 years and has been commonly used as a laxative. It is the radix and rhizome of Rheum officinale Baill., R. palmatum L. and R. tanguticum Maxim, all of which are mainly distributed in a broad region in the Tibetan plateau. Anthraquinone Glycosides are a series of major active ingredients found in all three species. They are key intermediates in the Anthraquinone secondary metabolism and the sennnoside biosynthesis. The variation of the Anthraquinone glycoside content in rhubarb in response to specific factors remains an attractive topic. A simple and sensitive Ultra Performance Liquid Chromatography with Photo-Diode Array (UPLC-PDA) detector was developed for the simultaneous determination of six Anthraquinone Glycosides in rhubarb, i.e., aloeemodin-8-O-glucoside, rhein-8-O-glucoside, chrysophanol-1-O-glucoside, emodin-1-O-glucoside, chrysophanol-8-O-glucoside, emodin-8-O-glucoside. Twenty-seven batches from three species were submitted to the multi-component analysis. The results showed that the Anthraquinone glycoside content varied significantly even within the same species. The results showed that the Anthraquinone glycoside content varied significantly within the same species but not between different species. The PCA and content analysis results confirmed that the plant species has no obvious effect on the content variation. Neither was any significant correlation observed between the Anthraquinone glycoside content and the geographic distribution of the rhubarb. Through correlational analysis, altitude was found to be the main factor that affects the Anthraquinone glycoside content in rhubarb. Rhubarb grown at higher altitude has higher Anthraquinone glycoside content. This work provides a rapid, sensitive and accurate UPLC-PDA method for the simultaneous determination of six Anthraquinone Glycosides in rhubarb. The Anthraquinone glycoside content varied significantly within the same species. The relationship of the Anthraquinone glycoside content with plant species, geographic distribution and altitude were studied using correlational analysis, principal component analysis and spatial autocorrelation analysis through SPSS and ArcGIS. Plant species and geographic distribution were found not to affect the content of the six Anthraquinone Glycosides in rhubarb. The variations in the Anthraquinone glycoside content were primarily due to the different altitude where the plant was grown.