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Yuegang Zuo - One of the best experts on this subject based on the ideXlab platform.
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ultrasonic extraction and hplc determination of Anthraquinones aloe emodine emodine rheine chrysophanol and physcione in roots of polygoni multiflori
Phytochemical Analysis, 2009Co-Authors: Yue Jiao, Yuegang ZuoAbstract:Introduction Polygoni multiflori, one of traditional Chinese herbal medicines for the treatment of various diseases commonly associated with aging, is known to contain active anthraquinone ingredients. However, the content of the Anthraquinones varies among P. multiflori samples with collection season and sites. Thus, simple, reliable and accurate analytical methods for determining of Anthraquinones in P. multiflori products are needed for the quality control and pharmacological studies. Objective To develop an HPLC method for the simultaneous determination of five Anthraquinones, aloe-emodine, rheine, emodine, chrysophanol and physcione, in the roots of P. multiflori. Methodology Anthraquinones were extracted from the roots of P. multiflori using aqueous alcohol solutions or hot water under ultrasonication. Separation and quantitation of Anthraquinones was accomplished using a reversed-phase C18 column with the mobile phase of methanol–water–phosphoric acid (600:400:1), and the detection wavelength of 254 nm. Results Seventy per cent aqueous ethanol showed the highest extraction efficiency for Anthraquinones from roots of P. multiflori when compared with four other extraction solvents tested. All calibration curves were linear over the concentration range tested with the square of correlation coefficients >0.999. The detection limits (S/N = 3) were 0.89, 1.1, 1.6, 1.7 and 2.0 ng for chrysophanol, aloe-emodine, rheine, emodine and physcione, respectively. Emodine and physcione were found in the samples tested at concentrations of 0.341 and 0.197 mg/g, respectively. Conclusion The described HPLC methods are simple, accurate and selective techniques for separation and quantification of Anthraquinones in roots of P. multiflori and other plant samples. Copyright © 2009 John Wiley & Sons, Ltd.
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simultaneous determination of Anthraquinones in radix polygoni multiflori by capillary gas chromatography coupled with flame ionization and mass spectrometric detection
Journal of Chromatography A, 2008Co-Authors: Yuegang Zuo, Chengjun Wang, Yuejuan Lin, Jinwen Guo, Yiwei DengAbstract:Abstract A rapid, accurate and reliable analytical method was developed for the simultaneous determination of five major Anthraquinones, aloe-emodin, chrysophanol, emodin, physcion, and rhein, in radix Polygoni multiflori, a traditional Chinese herbal medicine. The method comprises a fast ultrasonic extraction with methanol and derivatization with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) + 1% trimethylchlorosilane (TMCS) followed by capillary gas chromatographic (GC) separation. The effect of reaction time on the derivatization of Anthraquinones was examined. A baseline separation of the anthraquinone and internal standard derivatives was achieved in 15 min. The detection limits range from 0.22 to 0.60 μg/mL for the five Anthraquinones. The calibration curves are linear over the concentration range studied (from the detection limits to 40.0 μg/mL) with the squares of correlation coefficients, R2, greater than 0.998. The developed method was successfully applied to the simultaneous determination of Anthraquinones in radix P. multiflori samples. The peak identification was confirmed using GC–MS. The contents of Anthraquinones in radix P. multiflori samples studied were 27.41, 289.6, 64.22, 202.1, 288.6 μg/g for chrysophanol, emodin, aloe-emodin, physcion, rhein, respectively. All relative standard deviations are less than 3.2%. The recoveries range from 80.2% to 119.3% for the five analytes. To the authors’ best knowledge, this is the first GC method reported for the simultaneous determination of the five Anthraquinones in radix P. multiflori.
Marcello Locatelli - One of the best experts on this subject based on the ideXlab platform.
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comparison of three different extraction methods and hplc determination of the Anthraquinones aloe emodine emodine rheine chrysophanol and physcione in the bark of rhamnus alpinus l rhamnaceae
Phytochemical Analysis, 2010Co-Authors: Salvatore Genovese, Fabiana Tammaro, Giuseppe Carlucci, Francesco Epifano, Luigi Menghini, Marcello LocatelliAbstract:Introduction – Rhamnus alpinus L. (Rhamnaceae), a traditional plants in the flora of the Abruzzo region, is known to contain active anthraquinone secondary metabolites. However, the content of Anthraquinones varies among R. alpinus samples depending on collection season and site. Thus, using simple, reliable and accurate analytical methods for the determination of Anthraquinones in R. alpinus extracts allows comparative study of different methods of extraction. Objective – After a partial validation of an HPLC method for the simultaneous determination of five Anthraquinones, aloe-emodine, rheine, emodine, chrysophanol and physcione, in the bark of R. alpinus, we compared three different methods of extraction. Methodology – Anthraquinones were extracted from the bark of R. alpinus using different techniques (methanol maceration, ultrasonic and supercritical CO2 extraction). Separation and quantification of Anthraquinones were accomplished using a reversed-phase C18 column with the mobile phase of H2O–methanol (40 : 60, v/v, 1% formic acid) at a wavelength of 254 nm. The qualitative analyses were also achieved at wavelength of 435 nm. Results – All calibration curves were linear over the concentration range tested (10–200 mM) with the determination coefficients ≥0.991. The detection limits (S/N = 3) were 5 mM for each analytes. All five Anthraquinones were found in the samples tested at concentrations reported in experimental data. Conclusion – The described HPLC method and optimised extraction procedure are simple, accurate and selective for separation and quantification of Anthraquinones in the bark of R. alpinus and allow evaluation of the best extraction procedure between the tested assays. Copyright © 2009 John Wiley & Sons, Ltd.
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anthraquinone profile and chemical fingerprint of rhamnus saxatilis l from italy
Phytochemistry Letters, 2009Co-Authors: Marcello Locatelli, Fabiana Tammaro, Giuseppe Carlucci, Francesco Epifano, Luigi Menghini, Salvatore GenoveseAbstract:Abstract Several species belonging to the genus Rhamnus (Rhamnaceae), comprising ones among which are found the most typical plants of the Italian flora, are known to contain biologically active anthraquinone secondary metabolites. Although several Rhamnus species were so far investigated, no information is available concerning the content and relative abundances of Anthraquinones in R. saxatilis. In this study we used a simple, reliable, and accurate analytical method to determine the Anthraquinones in bark of R. saxatilis. This allowed us also to trace a comparative study on the efficacy of different extraction solvents in ultrasonication time dependent assays. Separation and quantification of Anthraquinones were accomplished using a C18 column with the mobile phase of H2O:methanol (40:60, v/v, 1% formic acid) at a flow rate of 0.7 mL/min and a detection wavelength of 254 nm, while the qualitative analyses were also achieved at a wavelength of 435 nm.Finally, the described HPLC method, was used to obtain a specific chemical fingerprint for this species in comparison with other species from the same family.
Yue Jiao - One of the best experts on this subject based on the ideXlab platform.
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ultrasonic extraction and hplc determination of Anthraquinones aloe emodine emodine rheine chrysophanol and physcione in roots of polygoni multiflori
Phytochemical Analysis, 2009Co-Authors: Yue Jiao, Yuegang ZuoAbstract:Introduction Polygoni multiflori, one of traditional Chinese herbal medicines for the treatment of various diseases commonly associated with aging, is known to contain active anthraquinone ingredients. However, the content of the Anthraquinones varies among P. multiflori samples with collection season and sites. Thus, simple, reliable and accurate analytical methods for determining of Anthraquinones in P. multiflori products are needed for the quality control and pharmacological studies. Objective To develop an HPLC method for the simultaneous determination of five Anthraquinones, aloe-emodine, rheine, emodine, chrysophanol and physcione, in the roots of P. multiflori. Methodology Anthraquinones were extracted from the roots of P. multiflori using aqueous alcohol solutions or hot water under ultrasonication. Separation and quantitation of Anthraquinones was accomplished using a reversed-phase C18 column with the mobile phase of methanol–water–phosphoric acid (600:400:1), and the detection wavelength of 254 nm. Results Seventy per cent aqueous ethanol showed the highest extraction efficiency for Anthraquinones from roots of P. multiflori when compared with four other extraction solvents tested. All calibration curves were linear over the concentration range tested with the square of correlation coefficients >0.999. The detection limits (S/N = 3) were 0.89, 1.1, 1.6, 1.7 and 2.0 ng for chrysophanol, aloe-emodine, rheine, emodine and physcione, respectively. Emodine and physcione were found in the samples tested at concentrations of 0.341 and 0.197 mg/g, respectively. Conclusion The described HPLC methods are simple, accurate and selective techniques for separation and quantification of Anthraquinones in roots of P. multiflori and other plant samples. Copyright © 2009 John Wiley & Sons, Ltd.
So Youn Won - One of the best experts on this subject based on the ideXlab platform.
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genome enabled discovery of anthraquinone biosynthesis in senna tora
Nature Communications, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, Daniel Ginzburg, Kangmei Zhao, So Youn WonAbstract:Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant anthraquinone biosynthesis, we reveal the genome sequence of S. tora at the chromosome level with 526 Mb (96%) assembled into 13 chromosomes. Comparison among related plant species shows that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identify a CHS-L gene contributing to the biosynthesis of Anthraquinones. The S. tora reference genome will accelerate the discovery of biologically active anthraquinone biosynthesis pathways in medicinal plants.
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de novo transcriptome sequence of senna tora provides insights into anthraquinone biosynthesis
PLOS ONE, 2020Co-Authors: Sang-ho Kang, Chang-muk Lee, Joon-soo Sim, So Youn Won, Woohaeng Lee, Sora Han, Soojin Kwon, Jung Sun Kim, Changkug KimAbstract:Senna tora is an annual herb with rich source of Anthraquinones that have tremendous pharmacological properties. However, there is little mention of genetic information for this species, especially regarding the biosynthetic pathways of Anthraquinones. To understand the key genes and regulatory mechanism of anthraquinone biosynthesis pathways, we performed spatial and temporal transcriptome sequencing of S. tora using short RNA sequencing (RNA-Seq) and long-read isoform sequencing (Iso-Seq) technologies, and generated two unigene sets composed of 118,635 and 39,364, respectively. A comprehensive functional annotation and classification with multiple public databases identified array of genes involved in major secondary metabolite biosynthesis pathways and important transcription factor (TF) families (MYB, MYB-related, AP2/ERF, C2C2-YABBY, and bHLH). Differential expression analysis indicated that the expression level of genes involved in anthraquinone biosynthetic pathway regulates differently depending on the degree of tissues and seeds development. Furthermore, we identified that the amount of anthraquinone compounds were greater in late seeds than early ones. In conclusion, these results provide a rich resource for understanding the anthraquinone metabolism in S. tora.
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genome enabled discovery of anthraquinone biosynthesis in senna tora
bioRxiv, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, So Youn Won, Namhoon Kim, Ok Ran LeeAbstract:Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant anthraquinone biosynthesis, we sequenced and annotated the genome of S. tora at the chromosome level with contig N50 and super-scaffold N50 of 4.03 Mb and 41.7 Mb. Comparison among related plant species showed that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identified a CHS-L responsible for biosynthesis of Anthraquinones, the first example in plants. The S. tora reference genome will accelerate the discovery of biologically active anthraquinone biosynthesis pathways in medicinal plants.
Sang-ho Kang - One of the best experts on this subject based on the ideXlab platform.
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genome enabled discovery of anthraquinone biosynthesis in senna tora
Nature Communications, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, Daniel Ginzburg, Kangmei Zhao, So Youn WonAbstract:Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant anthraquinone biosynthesis, we reveal the genome sequence of S. tora at the chromosome level with 526 Mb (96%) assembled into 13 chromosomes. Comparison among related plant species shows that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identify a CHS-L gene contributing to the biosynthesis of Anthraquinones. The S. tora reference genome will accelerate the discovery of biologically active anthraquinone biosynthesis pathways in medicinal plants.
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de novo transcriptome sequence of senna tora provides insights into anthraquinone biosynthesis
PLOS ONE, 2020Co-Authors: Sang-ho Kang, Chang-muk Lee, Joon-soo Sim, So Youn Won, Woohaeng Lee, Sora Han, Soojin Kwon, Jung Sun Kim, Changkug KimAbstract:Senna tora is an annual herb with rich source of Anthraquinones that have tremendous pharmacological properties. However, there is little mention of genetic information for this species, especially regarding the biosynthetic pathways of Anthraquinones. To understand the key genes and regulatory mechanism of anthraquinone biosynthesis pathways, we performed spatial and temporal transcriptome sequencing of S. tora using short RNA sequencing (RNA-Seq) and long-read isoform sequencing (Iso-Seq) technologies, and generated two unigene sets composed of 118,635 and 39,364, respectively. A comprehensive functional annotation and classification with multiple public databases identified array of genes involved in major secondary metabolite biosynthesis pathways and important transcription factor (TF) families (MYB, MYB-related, AP2/ERF, C2C2-YABBY, and bHLH). Differential expression analysis indicated that the expression level of genes involved in anthraquinone biosynthetic pathway regulates differently depending on the degree of tissues and seeds development. Furthermore, we identified that the amount of anthraquinone compounds were greater in late seeds than early ones. In conclusion, these results provide a rich resource for understanding the anthraquinone metabolism in S. tora.
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genome enabled discovery of anthraquinone biosynthesis in senna tora
bioRxiv, 2020Co-Authors: Sang-ho Kang, Ramesh Prasad Pandey, Chang-muk Lee, Joon-soo Sim, Jin-tae Jeong, Beom-soon Choi, Myunghee Jung, So Youn Won, Namhoon Kim, Ok Ran LeeAbstract:Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of Anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant anthraquinone biosynthesis, we sequenced and annotated the genome of S. tora at the chromosome level with contig N50 and super-scaffold N50 of 4.03 Mb and 41.7 Mb. Comparison among related plant species showed that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identified a CHS-L responsible for biosynthesis of Anthraquinones, the first example in plants. The S. tora reference genome will accelerate the discovery of biologically active anthraquinone biosynthesis pathways in medicinal plants.