The Experts below are selected from a list of 3282 Experts worldwide ranked by ideXlab platform
Olivier Pruvost - One of the best experts on this subject based on the ideXlab platform.
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A new semi-selective medium for the isolation of Xanthomonas axonopodis pv. Dieffenbachiae, the etiological agent of Anthurium bacterial blight
Letters in Applied Microbiology, 2009Co-Authors: Philippe Laurent, Aude Chabirand, Emmanuel Jouen, Isabelle Robene-soustrade, Lionel Gagnevin, Bruno Hostachy, Olivier PruvostAbstract:Aims:Xanthomonas axonopodis pv. dieffenbachiae causes Anthurium blight, which is regarded as the most threatening disease for the Anthurium industry worldwide. The bacterium is listed as a quarantine pathogen in several regions, including Europe. We evaluated the use of Neomycin-Cephalexin-Trimethoprime-pivMecillinam 4 (NCTM4) medium for its isolation. Methods and Results:A total of 104 bacterial strains were inoculated onto NCTM4 and on the previously published Cellobiose-Starch (CS) and Esculin-Trehalose (ET) media. The strain collection included: the Anthurium blight pathogen, Xanthomonas strains, for which false positive results are known to occur using serological identification-tests; other bacterial pathogens of Anthurium; and representatives of bacteria that are commonly present in the Anthurium phyllosphere. Media were evaluated following the ISO 16140 protocol for the validation of alternative methods. Conclusion:Growth of the Anthurium blight pathogen was better on NCTM4 and ET media than on CS. NCTM4 provided a better repeatability. It also displayed a lower rate of false positive and false negative results when the pathogen was isolated from plant extracts. Significance and Impact of the Study:This study will lead to improved isolation protocols of the Anthurium blight in official procedures. NCTM4 medium could also favourably be used in studies, which aim to further understanding of the biology and epidemiology of this pathogen.
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Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium (Anthurium andreanum) Tissues by Nested PCR
Applied and environmental microbiology, 2006Co-Authors: Isabelle Robene-soustrade, Philippe Laurent, Emmanuel Jouen, Lionel Gagnevin, Olivier PruvostAbstract:Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of Anthurium bacterial blight, requires a sensitive and reliable diagnostic tool. A nested PCR test was developed from a sequence-characterized amplified region marker identified by randomly amplified polymorphic DNA PCR for the detection of X. axonopodis pv. dieffenbachiae. Serological and pathogenicity tests were performed concurrently with the nested PCR test with a large collection of X. axonopodis pv. dieffenbachiae strains that were isolated worldwide and are pathogenic to Anthurium and/or other aroids. The internal primer pair directed amplification of the expected product (785 bp) for all 70 X. axonopodis pv. dieffenbachiae strains pathogenic to Anthurium tested and for isolates originating from syngonium and not pathogenic to Anthurium. This finding is consistent with previous studies which indicated that there is a high level of relatedness between strains from Anthurium and strains from syngonium. Strains originating from the two host genera can be distinguished by restriction analysis of the amplification product. No amplification product was obtained with 98 strains of unrelated phytopathogenic bacteria or saprophytic bacteria from the Anthurium phyllosphere, except for a weak signal obtained for one X. axonopodis pv. allii strain. Nevertheless, restriction enzyme analysis permitted the two pathovars to be distinguished. The detection threshold obtained with pure cultures or plant extracts (103 CFU ml−1) allowed detection of the pathogen from symptomless contaminated plants. This test could be a useful diagnostic tool for screening propagation stock plant material and for monitoring international movement of X. axonopodis pv. dieffenbachiae.
Isabelle Robene-soustrade - One of the best experts on this subject based on the ideXlab platform.
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A new semi-selective medium for the isolation of Xanthomonas axonopodis pv. Dieffenbachiae, the etiological agent of Anthurium bacterial blight
Letters in Applied Microbiology, 2009Co-Authors: Philippe Laurent, Aude Chabirand, Emmanuel Jouen, Isabelle Robene-soustrade, Lionel Gagnevin, Bruno Hostachy, Olivier PruvostAbstract:Aims:Xanthomonas axonopodis pv. dieffenbachiae causes Anthurium blight, which is regarded as the most threatening disease for the Anthurium industry worldwide. The bacterium is listed as a quarantine pathogen in several regions, including Europe. We evaluated the use of Neomycin-Cephalexin-Trimethoprime-pivMecillinam 4 (NCTM4) medium for its isolation. Methods and Results:A total of 104 bacterial strains were inoculated onto NCTM4 and on the previously published Cellobiose-Starch (CS) and Esculin-Trehalose (ET) media. The strain collection included: the Anthurium blight pathogen, Xanthomonas strains, for which false positive results are known to occur using serological identification-tests; other bacterial pathogens of Anthurium; and representatives of bacteria that are commonly present in the Anthurium phyllosphere. Media were evaluated following the ISO 16140 protocol for the validation of alternative methods. Conclusion:Growth of the Anthurium blight pathogen was better on NCTM4 and ET media than on CS. NCTM4 provided a better repeatability. It also displayed a lower rate of false positive and false negative results when the pathogen was isolated from plant extracts. Significance and Impact of the Study:This study will lead to improved isolation protocols of the Anthurium blight in official procedures. NCTM4 medium could also favourably be used in studies, which aim to further understanding of the biology and epidemiology of this pathogen.
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Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium (Anthurium andreanum) Tissues by Nested PCR
Applied and environmental microbiology, 2006Co-Authors: Isabelle Robene-soustrade, Philippe Laurent, Emmanuel Jouen, Lionel Gagnevin, Olivier PruvostAbstract:Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of Anthurium bacterial blight, requires a sensitive and reliable diagnostic tool. A nested PCR test was developed from a sequence-characterized amplified region marker identified by randomly amplified polymorphic DNA PCR for the detection of X. axonopodis pv. dieffenbachiae. Serological and pathogenicity tests were performed concurrently with the nested PCR test with a large collection of X. axonopodis pv. dieffenbachiae strains that were isolated worldwide and are pathogenic to Anthurium and/or other aroids. The internal primer pair directed amplification of the expected product (785 bp) for all 70 X. axonopodis pv. dieffenbachiae strains pathogenic to Anthurium tested and for isolates originating from syngonium and not pathogenic to Anthurium. This finding is consistent with previous studies which indicated that there is a high level of relatedness between strains from Anthurium and strains from syngonium. Strains originating from the two host genera can be distinguished by restriction analysis of the amplification product. No amplification product was obtained with 98 strains of unrelated phytopathogenic bacteria or saprophytic bacteria from the Anthurium phyllosphere, except for a weak signal obtained for one X. axonopodis pv. allii strain. Nevertheless, restriction enzyme analysis permitted the two pathovars to be distinguished. The detection threshold obtained with pure cultures or plant extracts (103 CFU ml−1) allowed detection of the pathogen from symptomless contaminated plants. This test could be a useful diagnostic tool for screening propagation stock plant material and for monitoring international movement of X. axonopodis pv. dieffenbachiae.
Philippe Laurent - One of the best experts on this subject based on the ideXlab platform.
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A new semi-selective medium for the isolation of Xanthomonas axonopodis pv. Dieffenbachiae, the etiological agent of Anthurium bacterial blight
Letters in Applied Microbiology, 2009Co-Authors: Philippe Laurent, Aude Chabirand, Emmanuel Jouen, Isabelle Robene-soustrade, Lionel Gagnevin, Bruno Hostachy, Olivier PruvostAbstract:Aims:Xanthomonas axonopodis pv. dieffenbachiae causes Anthurium blight, which is regarded as the most threatening disease for the Anthurium industry worldwide. The bacterium is listed as a quarantine pathogen in several regions, including Europe. We evaluated the use of Neomycin-Cephalexin-Trimethoprime-pivMecillinam 4 (NCTM4) medium for its isolation. Methods and Results:A total of 104 bacterial strains were inoculated onto NCTM4 and on the previously published Cellobiose-Starch (CS) and Esculin-Trehalose (ET) media. The strain collection included: the Anthurium blight pathogen, Xanthomonas strains, for which false positive results are known to occur using serological identification-tests; other bacterial pathogens of Anthurium; and representatives of bacteria that are commonly present in the Anthurium phyllosphere. Media were evaluated following the ISO 16140 protocol for the validation of alternative methods. Conclusion:Growth of the Anthurium blight pathogen was better on NCTM4 and ET media than on CS. NCTM4 provided a better repeatability. It also displayed a lower rate of false positive and false negative results when the pathogen was isolated from plant extracts. Significance and Impact of the Study:This study will lead to improved isolation protocols of the Anthurium blight in official procedures. NCTM4 medium could also favourably be used in studies, which aim to further understanding of the biology and epidemiology of this pathogen.
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Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium (Anthurium andreanum) Tissues by Nested PCR
Applied and environmental microbiology, 2006Co-Authors: Isabelle Robene-soustrade, Philippe Laurent, Emmanuel Jouen, Lionel Gagnevin, Olivier PruvostAbstract:Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of Anthurium bacterial blight, requires a sensitive and reliable diagnostic tool. A nested PCR test was developed from a sequence-characterized amplified region marker identified by randomly amplified polymorphic DNA PCR for the detection of X. axonopodis pv. dieffenbachiae. Serological and pathogenicity tests were performed concurrently with the nested PCR test with a large collection of X. axonopodis pv. dieffenbachiae strains that were isolated worldwide and are pathogenic to Anthurium and/or other aroids. The internal primer pair directed amplification of the expected product (785 bp) for all 70 X. axonopodis pv. dieffenbachiae strains pathogenic to Anthurium tested and for isolates originating from syngonium and not pathogenic to Anthurium. This finding is consistent with previous studies which indicated that there is a high level of relatedness between strains from Anthurium and strains from syngonium. Strains originating from the two host genera can be distinguished by restriction analysis of the amplification product. No amplification product was obtained with 98 strains of unrelated phytopathogenic bacteria or saprophytic bacteria from the Anthurium phyllosphere, except for a weak signal obtained for one X. axonopodis pv. allii strain. Nevertheless, restriction enzyme analysis permitted the two pathovars to be distinguished. The detection threshold obtained with pure cultures or plant extracts (103 CFU ml−1) allowed detection of the pathogen from symptomless contaminated plants. This test could be a useful diagnostic tool for screening propagation stock plant material and for monitoring international movement of X. axonopodis pv. dieffenbachiae.
Emmanuel Jouen - One of the best experts on this subject based on the ideXlab platform.
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A new semi-selective medium for the isolation of Xanthomonas axonopodis pv. Dieffenbachiae, the etiological agent of Anthurium bacterial blight
Letters in Applied Microbiology, 2009Co-Authors: Philippe Laurent, Aude Chabirand, Emmanuel Jouen, Isabelle Robene-soustrade, Lionel Gagnevin, Bruno Hostachy, Olivier PruvostAbstract:Aims:Xanthomonas axonopodis pv. dieffenbachiae causes Anthurium blight, which is regarded as the most threatening disease for the Anthurium industry worldwide. The bacterium is listed as a quarantine pathogen in several regions, including Europe. We evaluated the use of Neomycin-Cephalexin-Trimethoprime-pivMecillinam 4 (NCTM4) medium for its isolation. Methods and Results:A total of 104 bacterial strains were inoculated onto NCTM4 and on the previously published Cellobiose-Starch (CS) and Esculin-Trehalose (ET) media. The strain collection included: the Anthurium blight pathogen, Xanthomonas strains, for which false positive results are known to occur using serological identification-tests; other bacterial pathogens of Anthurium; and representatives of bacteria that are commonly present in the Anthurium phyllosphere. Media were evaluated following the ISO 16140 protocol for the validation of alternative methods. Conclusion:Growth of the Anthurium blight pathogen was better on NCTM4 and ET media than on CS. NCTM4 provided a better repeatability. It also displayed a lower rate of false positive and false negative results when the pathogen was isolated from plant extracts. Significance and Impact of the Study:This study will lead to improved isolation protocols of the Anthurium blight in official procedures. NCTM4 medium could also favourably be used in studies, which aim to further understanding of the biology and epidemiology of this pathogen.
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Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium (Anthurium andreanum) Tissues by Nested PCR
Applied and environmental microbiology, 2006Co-Authors: Isabelle Robene-soustrade, Philippe Laurent, Emmanuel Jouen, Lionel Gagnevin, Olivier PruvostAbstract:Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of Anthurium bacterial blight, requires a sensitive and reliable diagnostic tool. A nested PCR test was developed from a sequence-characterized amplified region marker identified by randomly amplified polymorphic DNA PCR for the detection of X. axonopodis pv. dieffenbachiae. Serological and pathogenicity tests were performed concurrently with the nested PCR test with a large collection of X. axonopodis pv. dieffenbachiae strains that were isolated worldwide and are pathogenic to Anthurium and/or other aroids. The internal primer pair directed amplification of the expected product (785 bp) for all 70 X. axonopodis pv. dieffenbachiae strains pathogenic to Anthurium tested and for isolates originating from syngonium and not pathogenic to Anthurium. This finding is consistent with previous studies which indicated that there is a high level of relatedness between strains from Anthurium and strains from syngonium. Strains originating from the two host genera can be distinguished by restriction analysis of the amplification product. No amplification product was obtained with 98 strains of unrelated phytopathogenic bacteria or saprophytic bacteria from the Anthurium phyllosphere, except for a weak signal obtained for one X. axonopodis pv. allii strain. Nevertheless, restriction enzyme analysis permitted the two pathovars to be distinguished. The detection threshold obtained with pure cultures or plant extracts (103 CFU ml−1) allowed detection of the pathogen from symptomless contaminated plants. This test could be a useful diagnostic tool for screening propagation stock plant material and for monitoring international movement of X. axonopodis pv. dieffenbachiae.
Lionel Gagnevin - One of the best experts on this subject based on the ideXlab platform.
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A new semi-selective medium for the isolation of Xanthomonas axonopodis pv. Dieffenbachiae, the etiological agent of Anthurium bacterial blight
Letters in Applied Microbiology, 2009Co-Authors: Philippe Laurent, Aude Chabirand, Emmanuel Jouen, Isabelle Robene-soustrade, Lionel Gagnevin, Bruno Hostachy, Olivier PruvostAbstract:Aims:Xanthomonas axonopodis pv. dieffenbachiae causes Anthurium blight, which is regarded as the most threatening disease for the Anthurium industry worldwide. The bacterium is listed as a quarantine pathogen in several regions, including Europe. We evaluated the use of Neomycin-Cephalexin-Trimethoprime-pivMecillinam 4 (NCTM4) medium for its isolation. Methods and Results:A total of 104 bacterial strains were inoculated onto NCTM4 and on the previously published Cellobiose-Starch (CS) and Esculin-Trehalose (ET) media. The strain collection included: the Anthurium blight pathogen, Xanthomonas strains, for which false positive results are known to occur using serological identification-tests; other bacterial pathogens of Anthurium; and representatives of bacteria that are commonly present in the Anthurium phyllosphere. Media were evaluated following the ISO 16140 protocol for the validation of alternative methods. Conclusion:Growth of the Anthurium blight pathogen was better on NCTM4 and ET media than on CS. NCTM4 provided a better repeatability. It also displayed a lower rate of false positive and false negative results when the pathogen was isolated from plant extracts. Significance and Impact of the Study:This study will lead to improved isolation protocols of the Anthurium blight in official procedures. NCTM4 medium could also favourably be used in studies, which aim to further understanding of the biology and epidemiology of this pathogen.
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Specific Detection of Xanthomonas axonopodis pv. dieffenbachiae in Anthurium (Anthurium andreanum) Tissues by Nested PCR
Applied and environmental microbiology, 2006Co-Authors: Isabelle Robene-soustrade, Philippe Laurent, Emmanuel Jouen, Lionel Gagnevin, Olivier PruvostAbstract:Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of Anthurium bacterial blight, requires a sensitive and reliable diagnostic tool. A nested PCR test was developed from a sequence-characterized amplified region marker identified by randomly amplified polymorphic DNA PCR for the detection of X. axonopodis pv. dieffenbachiae. Serological and pathogenicity tests were performed concurrently with the nested PCR test with a large collection of X. axonopodis pv. dieffenbachiae strains that were isolated worldwide and are pathogenic to Anthurium and/or other aroids. The internal primer pair directed amplification of the expected product (785 bp) for all 70 X. axonopodis pv. dieffenbachiae strains pathogenic to Anthurium tested and for isolates originating from syngonium and not pathogenic to Anthurium. This finding is consistent with previous studies which indicated that there is a high level of relatedness between strains from Anthurium and strains from syngonium. Strains originating from the two host genera can be distinguished by restriction analysis of the amplification product. No amplification product was obtained with 98 strains of unrelated phytopathogenic bacteria or saprophytic bacteria from the Anthurium phyllosphere, except for a weak signal obtained for one X. axonopodis pv. allii strain. Nevertheless, restriction enzyme analysis permitted the two pathovars to be distinguished. The detection threshold obtained with pure cultures or plant extracts (103 CFU ml−1) allowed detection of the pathogen from symptomless contaminated plants. This test could be a useful diagnostic tool for screening propagation stock plant material and for monitoring international movement of X. axonopodis pv. dieffenbachiae.