The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Hideyuki Tanaka - One of the best experts on this subject based on the ideXlab platform.

  • free fatty acids regulate insulin secretion from pancreatic beta cells through gpr40
    Nature, 2003
    Co-Authors: Yasuaki Itoh, Yuji Kawamata, Masataka Harada, Makoto Kobayashi, Ryo Fujii, Shoji Fukusumi, Masaki Hosoya, Yasuhiro Tanaka, Hiroshi Uejima, Hideyuki Tanaka
    Abstract:

    Diabetes, a disease in which carbohydrate and lipid metabolism are regulated improperly by insulin, is a serious worldwide health issue1,2. Insulin is secreted from pancreatic β cells in response to elevated plasma glucose, with various factors modifying its secretion3. Free fatty acids (FFAs) provide an important energy source as nutrients, and they also act as signalling molecules in various cellular processes, including insulin secretion4,5. Although FFAs are thought to promote insulin secretion in an acute phase, this mechanism is not clearly understood6. Here we show that a G-protein-coupled receptor, GPR40, which is abundantly expressed in the pancreas, functions as a receptor for long-chain FFAs. Furthermore, we show that long-chain FFAs amplify glucose-stimulated insulin secretion from pancreatic β cells by activating GPR40. Our results indicate that GPR40 agonists and/or antagonists show potential for the development of new Anti-Diabetic Drugs.

  • free fatty acids regulate insulin secretion from pancreatic beta cells through gpr40
    Nature, 2003
    Co-Authors: Yasuaki Itoh, Yuji Kawamata, Masataka Harada, Makoto Kobayashi, Ryo Fujii, Shoji Fukusumi, Masaki Hosoya, Yasuhiro Tanaka, Hiroshi Uejima, Hideyuki Tanaka
    Abstract:

    Diabetes, a disease in which carbohydrate and lipid metabolism are regulated improperly by insulin, is a serious worldwide health issue. Insulin is secreted from pancreatic beta cells in response to elevated plasma glucose, with various factors modifying its secretion. Free fatty acids (FFAs) provide an important energy source as nutrients, and they also act as signalling molecules in various cellular processes, including insulin secretion. Although FFAs are thought to promote insulin secretion in an acute phase, this mechanism is not clearly understood. Here we show that a G-protein-coupled receptor, GPR40, which is abundantly expressed in the pancreas, functions as a receptor for long-chain FFAs. Furthermore, we show that long-chain FFAs amplify glucose-stimulated insulin secretion from pancreatic beta cells by activating GPR40. Our results indicate that GPR40 agonists and/or antagonists show potential for the development of new Anti-Diabetic Drugs.

Sung Won Kwon - One of the best experts on this subject based on the ideXlab platform.

  • comparison of ultraviolet detection evaporative light scattering detection and charged aerosol detection methods for liquid chromatographic determination of anti diabetic Drugs
    Journal of Pharmaceutical and Biomedical Analysis, 2010
    Co-Authors: Jia Shaodong, Ji Won Ee, Jeong Hill Park, Sung Won Kwon
    Abstract:

    Abstract Recently, charged aerosol detection (CAD), a new kind of universal detection method, has been widely employed in the HPLC system. In the present study, four kinds of Anti-Diabetic drug standards, glipizide, gliclazide, glibenclamide and glimepiride were determined by ultraviolet (UV) detection, evaporative light scattering detection (ELSD) and the aforementioned CAD. The results were compared with reference to linearity, accuracy, precision and limit of detection (LOD). All of the experiments were performed on a reverse phase column with water and acetonitrile as the mobile phase. Separations were achieved under the same chromatographic conditions for each detection method. As a result, CAD generated nearly uniform responses compared with UV detection and ELSD. It showed the best accuracy and LOD among 3 detectors and had similar precision with UV detection at higher concentrations while UV detection showed a better precision at lower concentrations than did CAD or ELSD. The LOD of CAD, in fact, can be up to two times higher than that of ELSD. The UV and ELSD linearity was satisfactory at R2 > 0.99, though in the case of CAD, a log–log transformation was needed. The proposed methods were also applied to the real Anti-Diabetic Drugs and diabetes-related dietary supplements.

  • comparison of ultraviolet detection evaporative light scattering detection and charged aerosol detection methods for liquid chromatographic determination of anti diabetic Drugs
    Journal of Pharmaceutical and Biomedical Analysis, 2010
    Co-Authors: Jia Shaodong, Jeong Hill Park, Sung Won Kwon, Won Jun Lee, Jeongmi Lee
    Abstract:

    Recently, charged aerosol detection (CAD), a new kind of universal detection method, has been widely employed in the HPLC system. In the present study, four kinds of Anti-Diabetic drug standards, glipizide, gliclazide, glibenclamide and glimepiride were determined by ultraviolet (UV) detection, evaporative light scattering detection (ELSD) and the aforementioned CAD. The results were compared with reference to linearity, accuracy, precision and limit of detection (LOD). All of the experiments were performed on a reverse phase column with water and acetonitrile as the mobile phase. Separations were achieved under the same chromatographic conditions for each detection method. As a result, CAD generated nearly uniform responses compared with UV detection and ELSD. It showed the best accuracy and LOD among 3 detectors and had similar precision with UV detection at higher concentrations while UV detection showed a better precision at lower concentrations than did CAD or ELSD. The LOD of CAD, in fact, can be up to two times higher than that of ELSD. The UV and ELSD linearity was satisfactory at R(2)>0.99, though in the case of CAD, a log-log transformation was needed. The proposed methods were also applied to the real Anti-Diabetic Drugs and diabetes-related dietary supplements.

  • simultaneous determination of anti diabetes anti obesity Drugs by lc pda and targeted analysis of sibutramine analog in dietary supplements by lc ms ms
    Biomedical Chromatography, 2009
    Co-Authors: So Hyun Kim, Jeongmi Lee, Taehyung Yoon, Jangduck Choi, Dongmi Choi, Deukjoon Kim, Sung Won Kwon
    Abstract:

    The safety of dietary supplements is questionable as there have been occasional reports of products contaminated with illegal adulterants. The present study was carried out to develop trustworthy methodologies to screen for six Anti-Diabetic Drugs (phenformin, rosiglitazone, glipizide, glimepiride, glybenclamide and gliclazide) and six anti-obesity Drugs (ephedrine, fenfluramine, T3, T4, fluoxetine and sibutramine) in dietary supplements. A simultaneous determination method of the 12 Drugs by liquid chromatography coupled with a photodiode array (LC/PDA) was established and was validated for linearity (r(2) > 0.99), precision (RSD <13.3%), recoveries (88.8-115.9%) and reproducibility. Sibutramine and its analogs, N-desmethylsibutramine, were subject to further investigation by LC/MS/MS because they were one of the major illegal adulterants. Our proposed method to monitor illegal drug adulterations in dietary supplements using LC/PDA is a simple and reliable, and therefore applicable to routine drug-adulteration screening.

Jia Shaodong - One of the best experts on this subject based on the ideXlab platform.

  • comparison of ultraviolet detection evaporative light scattering detection and charged aerosol detection methods for liquid chromatographic determination of anti diabetic Drugs
    Journal of Pharmaceutical and Biomedical Analysis, 2010
    Co-Authors: Jia Shaodong, Ji Won Ee, Jeong Hill Park, Sung Won Kwon
    Abstract:

    Abstract Recently, charged aerosol detection (CAD), a new kind of universal detection method, has been widely employed in the HPLC system. In the present study, four kinds of Anti-Diabetic drug standards, glipizide, gliclazide, glibenclamide and glimepiride were determined by ultraviolet (UV) detection, evaporative light scattering detection (ELSD) and the aforementioned CAD. The results were compared with reference to linearity, accuracy, precision and limit of detection (LOD). All of the experiments were performed on a reverse phase column with water and acetonitrile as the mobile phase. Separations were achieved under the same chromatographic conditions for each detection method. As a result, CAD generated nearly uniform responses compared with UV detection and ELSD. It showed the best accuracy and LOD among 3 detectors and had similar precision with UV detection at higher concentrations while UV detection showed a better precision at lower concentrations than did CAD or ELSD. The LOD of CAD, in fact, can be up to two times higher than that of ELSD. The UV and ELSD linearity was satisfactory at R2 > 0.99, though in the case of CAD, a log–log transformation was needed. The proposed methods were also applied to the real Anti-Diabetic Drugs and diabetes-related dietary supplements.

  • comparison of ultraviolet detection evaporative light scattering detection and charged aerosol detection methods for liquid chromatographic determination of anti diabetic Drugs
    Journal of Pharmaceutical and Biomedical Analysis, 2010
    Co-Authors: Jia Shaodong, Jeong Hill Park, Sung Won Kwon, Won Jun Lee, Jeongmi Lee
    Abstract:

    Recently, charged aerosol detection (CAD), a new kind of universal detection method, has been widely employed in the HPLC system. In the present study, four kinds of Anti-Diabetic drug standards, glipizide, gliclazide, glibenclamide and glimepiride were determined by ultraviolet (UV) detection, evaporative light scattering detection (ELSD) and the aforementioned CAD. The results were compared with reference to linearity, accuracy, precision and limit of detection (LOD). All of the experiments were performed on a reverse phase column with water and acetonitrile as the mobile phase. Separations were achieved under the same chromatographic conditions for each detection method. As a result, CAD generated nearly uniform responses compared with UV detection and ELSD. It showed the best accuracy and LOD among 3 detectors and had similar precision with UV detection at higher concentrations while UV detection showed a better precision at lower concentrations than did CAD or ELSD. The LOD of CAD, in fact, can be up to two times higher than that of ELSD. The UV and ELSD linearity was satisfactory at R(2)>0.99, though in the case of CAD, a log-log transformation was needed. The proposed methods were also applied to the real Anti-Diabetic Drugs and diabetes-related dietary supplements.

Yasuaki Itoh - One of the best experts on this subject based on the ideXlab platform.

  • free fatty acids regulate insulin secretion from pancreatic beta cells through gpr40
    Nature, 2003
    Co-Authors: Yasuaki Itoh, Yuji Kawamata, Masataka Harada, Makoto Kobayashi, Ryo Fujii, Shoji Fukusumi, Masaki Hosoya, Yasuhiro Tanaka, Hiroshi Uejima, Hideyuki Tanaka
    Abstract:

    Diabetes, a disease in which carbohydrate and lipid metabolism are regulated improperly by insulin, is a serious worldwide health issue1,2. Insulin is secreted from pancreatic β cells in response to elevated plasma glucose, with various factors modifying its secretion3. Free fatty acids (FFAs) provide an important energy source as nutrients, and they also act as signalling molecules in various cellular processes, including insulin secretion4,5. Although FFAs are thought to promote insulin secretion in an acute phase, this mechanism is not clearly understood6. Here we show that a G-protein-coupled receptor, GPR40, which is abundantly expressed in the pancreas, functions as a receptor for long-chain FFAs. Furthermore, we show that long-chain FFAs amplify glucose-stimulated insulin secretion from pancreatic β cells by activating GPR40. Our results indicate that GPR40 agonists and/or antagonists show potential for the development of new Anti-Diabetic Drugs.

  • free fatty acids regulate insulin secretion from pancreatic beta cells through gpr40
    Nature, 2003
    Co-Authors: Yasuaki Itoh, Yuji Kawamata, Masataka Harada, Makoto Kobayashi, Ryo Fujii, Shoji Fukusumi, Masaki Hosoya, Yasuhiro Tanaka, Hiroshi Uejima, Hideyuki Tanaka
    Abstract:

    Diabetes, a disease in which carbohydrate and lipid metabolism are regulated improperly by insulin, is a serious worldwide health issue. Insulin is secreted from pancreatic beta cells in response to elevated plasma glucose, with various factors modifying its secretion. Free fatty acids (FFAs) provide an important energy source as nutrients, and they also act as signalling molecules in various cellular processes, including insulin secretion. Although FFAs are thought to promote insulin secretion in an acute phase, this mechanism is not clearly understood. Here we show that a G-protein-coupled receptor, GPR40, which is abundantly expressed in the pancreas, functions as a receptor for long-chain FFAs. Furthermore, we show that long-chain FFAs amplify glucose-stimulated insulin secretion from pancreatic beta cells by activating GPR40. Our results indicate that GPR40 agonists and/or antagonists show potential for the development of new Anti-Diabetic Drugs.

Sanjay Kalra - One of the best experts on this subject based on the ideXlab platform.

  • sodium glucose cotransporter 2 inhibition and the glomerulus a review
    Advances in Therapy, 2016
    Co-Authors: Sanjay Kalra, Vikram Singh, Dinesh Nagrale
    Abstract:

    Blood glucose-lowering treatment options generally target insulin action or beta-cell function. In diabetes, expression of the sodium-glucose cotransporter-2 (SGLT2) genes is up-regulated and renal threshold increased, resulting in increased glucose reabsorption from glomerular filtrate, reducing urinary glucose excretion and worsening the hyperglycemic condition. The SGLT2 inhibitors (SGLT2i) are a novel class of Anti-Diabetic Drugs that lower blood glucose levels through the suppression of renal glucose reabsorption thereby promoting renal glucose excretion. The efficacy of SGLT2i is reduced in renal impairment because the ability of glucose-lowering is directly proportional to glomerular filtration rate. On the other hand, ongoing research suggests that SGLT2i may offer potential nephroprotection in diabetes. The SGLT2i have been shown to reduce glomerular hyperfiltration, systemic and intraglomerular pressure and the biochemical progression of chronic kidney disease. Additional mechanisms through which SGLT2i exert nephroprotection may include normalizing blood pressure and uricemia. This review explores this bidirectional relationship of the SGLT2i and the glomerulus. While SGLT2i exhibit reduced efficacy in later stages, they exhibit nephroprotective effects in early stages of renal impairment.

  • Sodium Glucose Co-Transporter-2 (SGLT2) Inhibitors: A Review of Their Basic and Clinical Pharmacology
    Diabetes Therapy, 2014
    Co-Authors: Sanjay Kalra
    Abstract:

    Sodium-glucose co-transporter-2 (SGLT2) inhibitors are a newly developed class of oral Anti-Diabetic Drugs (OADs) with a unique mechanism of action. This review describes the biochemistry and physiology underlying the use of SGLT2 inhibitors, and their clinical pharmacology, including mechanism of action and posology. The pragmatic placement of these molecules in the existing OAD arena is also discussed.