The Experts below are selected from a list of 15 Experts worldwide ranked by ideXlab platform
Huaguang Kang - One of the best experts on this subject based on the ideXlab platform.
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cholecystokinin octaPeptide reverses the κ opioid receptor mediated depression of calcium current in rat dorsal root ganglion neurons
Brain Research, 1996Co-Authors: Tao Xu, Yingxin Yu, Chongqing Li, Yu Shangguan, Huaguang KangAbstract:Abstract Although the cholecystokinin (CCK-8) is reported to antagonize the κ-opioid-receptor-mediated analgesic effect in spinal cord, its mechanism and sites of action remain obscure. In the present study, the whole-cell patch-clamp recording technique was employed to examine the effect of κ-opioid agonist U50488H on voltage-gated calcium channels and the interaction between the CCK-8 and U50488H in acutely isolated rat dorsal root ganglion neurons. The results indicate that the calcium currents elicited in dorsal root Antiopioid Peptide CCK-8. The effect of the CCK-8 can be abolished by the CCK-B receptor antagonist, L365, 260. While CCK-8 showed a potent opioid-reversal effect, it by itself exerted a slight inhibitory effects on calcium current. This novel observation in the dorsal root ganglion neurons indicates that CCK-8 can antagonize the κ-opioid-receptor-mediated depressant effect on voltage-gated calcium current, and this antagonizing effect appears to be mediated via CCK-B receptor.
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cholecystokinin octaPeptide reverses mu opioid receptor mediated inhibition of calcium current in rat dorsal root ganglion neurons
Journal of Pharmacology and Experimental Therapeutics, 1995Co-Authors: Tao Xu, Yingxin Yu, Chongqing Li, Chang Xu, Huaguang KangAbstract:Cholecystokinin octaPeptide (CCK-8) is reported to antagonize the analgesic effect produced by mu- and kappa- but not delta-opioid agonist in spinal cord. However, the mechanisms of interaction remain obscure. In the present study, whole-cell patch-clamp recording was performed on acutely isolated rat dorsal root ganglion (DRG) neurons to evaluate the effects of the highly specific mu-opioid agonist ohmefentanyl and the delta-opioid agonist DPDPE on voltage-gated calcium channels and the possible interaction between CCK-8 receptor and mu- or delta-opioid receptor. The results indicated that ohmefentanyl, but not DPDPE, can suppress the voltage-gated calcium currents elicited in DRG neurons, an effect readily reversed by naloxone or by the Antiopioid Peptide CCK-8. The effect of CCK-8 can in turn be abolished by the CCK-B receptor antagonist L365,260. CCK-8 used by itself has no enhancing effect, but rather a depressant effect, on calcium currents. However, used simultaneously with ohmefentanyl, CCK-8 shows a clear-cut reversal of depression of the mu-opioid. We conclude that the depressant effect produced by mu-opioid on voltage-gated calcium current in DRG neurons can be antagonized by CCK-8 through CCK-B receptor located in the same neuron. The delta-opioid DPDPE has no direct effect on the voltage-gated calcium current in DRG neurons.
Tao Xu - One of the best experts on this subject based on the ideXlab platform.
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cholecystokinin octaPeptide reverses the κ opioid receptor mediated depression of calcium current in rat dorsal root ganglion neurons
Brain Research, 1996Co-Authors: Tao Xu, Yingxin Yu, Chongqing Li, Yu Shangguan, Huaguang KangAbstract:Abstract Although the cholecystokinin (CCK-8) is reported to antagonize the κ-opioid-receptor-mediated analgesic effect in spinal cord, its mechanism and sites of action remain obscure. In the present study, the whole-cell patch-clamp recording technique was employed to examine the effect of κ-opioid agonist U50488H on voltage-gated calcium channels and the interaction between the CCK-8 and U50488H in acutely isolated rat dorsal root ganglion neurons. The results indicate that the calcium currents elicited in dorsal root Antiopioid Peptide CCK-8. The effect of the CCK-8 can be abolished by the CCK-B receptor antagonist, L365, 260. While CCK-8 showed a potent opioid-reversal effect, it by itself exerted a slight inhibitory effects on calcium current. This novel observation in the dorsal root ganglion neurons indicates that CCK-8 can antagonize the κ-opioid-receptor-mediated depressant effect on voltage-gated calcium current, and this antagonizing effect appears to be mediated via CCK-B receptor.
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cholecystokinin octaPeptide reverses mu opioid receptor mediated inhibition of calcium current in rat dorsal root ganglion neurons
Journal of Pharmacology and Experimental Therapeutics, 1995Co-Authors: Tao Xu, Yingxin Yu, Chongqing Li, Chang Xu, Huaguang KangAbstract:Cholecystokinin octaPeptide (CCK-8) is reported to antagonize the analgesic effect produced by mu- and kappa- but not delta-opioid agonist in spinal cord. However, the mechanisms of interaction remain obscure. In the present study, whole-cell patch-clamp recording was performed on acutely isolated rat dorsal root ganglion (DRG) neurons to evaluate the effects of the highly specific mu-opioid agonist ohmefentanyl and the delta-opioid agonist DPDPE on voltage-gated calcium channels and the possible interaction between CCK-8 receptor and mu- or delta-opioid receptor. The results indicated that ohmefentanyl, but not DPDPE, can suppress the voltage-gated calcium currents elicited in DRG neurons, an effect readily reversed by naloxone or by the Antiopioid Peptide CCK-8. The effect of CCK-8 can in turn be abolished by the CCK-B receptor antagonist L365,260. CCK-8 used by itself has no enhancing effect, but rather a depressant effect, on calcium currents. However, used simultaneously with ohmefentanyl, CCK-8 shows a clear-cut reversal of depression of the mu-opioid. We conclude that the depressant effect produced by mu-opioid on voltage-gated calcium current in DRG neurons can be antagonized by CCK-8 through CCK-B receptor located in the same neuron. The delta-opioid DPDPE has no direct effect on the voltage-gated calcium current in DRG neurons.
Yingxin Yu - One of the best experts on this subject based on the ideXlab platform.
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cholecystokinin octaPeptide reverses the κ opioid receptor mediated depression of calcium current in rat dorsal root ganglion neurons
Brain Research, 1996Co-Authors: Tao Xu, Yingxin Yu, Chongqing Li, Yu Shangguan, Huaguang KangAbstract:Abstract Although the cholecystokinin (CCK-8) is reported to antagonize the κ-opioid-receptor-mediated analgesic effect in spinal cord, its mechanism and sites of action remain obscure. In the present study, the whole-cell patch-clamp recording technique was employed to examine the effect of κ-opioid agonist U50488H on voltage-gated calcium channels and the interaction between the CCK-8 and U50488H in acutely isolated rat dorsal root ganglion neurons. The results indicate that the calcium currents elicited in dorsal root Antiopioid Peptide CCK-8. The effect of the CCK-8 can be abolished by the CCK-B receptor antagonist, L365, 260. While CCK-8 showed a potent opioid-reversal effect, it by itself exerted a slight inhibitory effects on calcium current. This novel observation in the dorsal root ganglion neurons indicates that CCK-8 can antagonize the κ-opioid-receptor-mediated depressant effect on voltage-gated calcium current, and this antagonizing effect appears to be mediated via CCK-B receptor.
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cholecystokinin octaPeptide reverses mu opioid receptor mediated inhibition of calcium current in rat dorsal root ganglion neurons
Journal of Pharmacology and Experimental Therapeutics, 1995Co-Authors: Tao Xu, Yingxin Yu, Chongqing Li, Chang Xu, Huaguang KangAbstract:Cholecystokinin octaPeptide (CCK-8) is reported to antagonize the analgesic effect produced by mu- and kappa- but not delta-opioid agonist in spinal cord. However, the mechanisms of interaction remain obscure. In the present study, whole-cell patch-clamp recording was performed on acutely isolated rat dorsal root ganglion (DRG) neurons to evaluate the effects of the highly specific mu-opioid agonist ohmefentanyl and the delta-opioid agonist DPDPE on voltage-gated calcium channels and the possible interaction between CCK-8 receptor and mu- or delta-opioid receptor. The results indicated that ohmefentanyl, but not DPDPE, can suppress the voltage-gated calcium currents elicited in DRG neurons, an effect readily reversed by naloxone or by the Antiopioid Peptide CCK-8. The effect of CCK-8 can in turn be abolished by the CCK-B receptor antagonist L365,260. CCK-8 used by itself has no enhancing effect, but rather a depressant effect, on calcium currents. However, used simultaneously with ohmefentanyl, CCK-8 shows a clear-cut reversal of depression of the mu-opioid. We conclude that the depressant effect produced by mu-opioid on voltage-gated calcium current in DRG neurons can be antagonized by CCK-8 through CCK-B receptor located in the same neuron. The delta-opioid DPDPE has no direct effect on the voltage-gated calcium current in DRG neurons.
Chongqing Li - One of the best experts on this subject based on the ideXlab platform.
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cholecystokinin octaPeptide reverses the κ opioid receptor mediated depression of calcium current in rat dorsal root ganglion neurons
Brain Research, 1996Co-Authors: Tao Xu, Yingxin Yu, Chongqing Li, Yu Shangguan, Huaguang KangAbstract:Abstract Although the cholecystokinin (CCK-8) is reported to antagonize the κ-opioid-receptor-mediated analgesic effect in spinal cord, its mechanism and sites of action remain obscure. In the present study, the whole-cell patch-clamp recording technique was employed to examine the effect of κ-opioid agonist U50488H on voltage-gated calcium channels and the interaction between the CCK-8 and U50488H in acutely isolated rat dorsal root ganglion neurons. The results indicate that the calcium currents elicited in dorsal root Antiopioid Peptide CCK-8. The effect of the CCK-8 can be abolished by the CCK-B receptor antagonist, L365, 260. While CCK-8 showed a potent opioid-reversal effect, it by itself exerted a slight inhibitory effects on calcium current. This novel observation in the dorsal root ganglion neurons indicates that CCK-8 can antagonize the κ-opioid-receptor-mediated depressant effect on voltage-gated calcium current, and this antagonizing effect appears to be mediated via CCK-B receptor.
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cholecystokinin octaPeptide reverses mu opioid receptor mediated inhibition of calcium current in rat dorsal root ganglion neurons
Journal of Pharmacology and Experimental Therapeutics, 1995Co-Authors: Tao Xu, Yingxin Yu, Chongqing Li, Chang Xu, Huaguang KangAbstract:Cholecystokinin octaPeptide (CCK-8) is reported to antagonize the analgesic effect produced by mu- and kappa- but not delta-opioid agonist in spinal cord. However, the mechanisms of interaction remain obscure. In the present study, whole-cell patch-clamp recording was performed on acutely isolated rat dorsal root ganglion (DRG) neurons to evaluate the effects of the highly specific mu-opioid agonist ohmefentanyl and the delta-opioid agonist DPDPE on voltage-gated calcium channels and the possible interaction between CCK-8 receptor and mu- or delta-opioid receptor. The results indicated that ohmefentanyl, but not DPDPE, can suppress the voltage-gated calcium currents elicited in DRG neurons, an effect readily reversed by naloxone or by the Antiopioid Peptide CCK-8. The effect of CCK-8 can in turn be abolished by the CCK-B receptor antagonist L365,260. CCK-8 used by itself has no enhancing effect, but rather a depressant effect, on calcium currents. However, used simultaneously with ohmefentanyl, CCK-8 shows a clear-cut reversal of depression of the mu-opioid. We conclude that the depressant effect produced by mu-opioid on voltage-gated calcium current in DRG neurons can be antagonized by CCK-8 through CCK-B receptor located in the same neuron. The delta-opioid DPDPE has no direct effect on the voltage-gated calcium current in DRG neurons.
Yu Shangguan - One of the best experts on this subject based on the ideXlab platform.
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cholecystokinin octaPeptide reverses the κ opioid receptor mediated depression of calcium current in rat dorsal root ganglion neurons
Brain Research, 1996Co-Authors: Tao Xu, Yingxin Yu, Chongqing Li, Yu Shangguan, Huaguang KangAbstract:Abstract Although the cholecystokinin (CCK-8) is reported to antagonize the κ-opioid-receptor-mediated analgesic effect in spinal cord, its mechanism and sites of action remain obscure. In the present study, the whole-cell patch-clamp recording technique was employed to examine the effect of κ-opioid agonist U50488H on voltage-gated calcium channels and the interaction between the CCK-8 and U50488H in acutely isolated rat dorsal root ganglion neurons. The results indicate that the calcium currents elicited in dorsal root Antiopioid Peptide CCK-8. The effect of the CCK-8 can be abolished by the CCK-B receptor antagonist, L365, 260. While CCK-8 showed a potent opioid-reversal effect, it by itself exerted a slight inhibitory effects on calcium current. This novel observation in the dorsal root ganglion neurons indicates that CCK-8 can antagonize the κ-opioid-receptor-mediated depressant effect on voltage-gated calcium current, and this antagonizing effect appears to be mediated via CCK-B receptor.