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Antioxidant Assay

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Riming Huang – One of the best experts on this subject based on the ideXlab platform.

  • new antioxidative secondary metabolites from the fruits of a beibu gulf mangrove avicennia marina
    Marine Drugs, 2014
    Co-Authors: Cheng-hai Gao, Wenpei Xie, Yinning Chen, Riming Huang

    Abstract:

    Further chemical investigation of the fruits of the mangrove, Avicennia marina, afforded three new phenylethyl glycosides, marinoids J-L (1-3), and a new cinnamoyl glycoside, marinoid M (4). The structures of isolates were elucidated on the basis of extensive spectroscopic analysis and by comparison of the data with those of related secondary metabolites. The Antioxidant activity of the isolates was evaluated using the cellular Antioxidant Assay (CAA), and compounds 1-4 showed Antioxidant activities, with EC50 values ranging from 23.0 ± 0.71 μM to 247.8 ± 2.47 μM.

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  • four new jacaranone analogs from the fruits of a beibu gulf mangrove avicennia marina
    Marine Drugs, 2014
    Co-Authors: Yong Chen, Cheng-hai Gao, Wenpei Xie, Yinning Chen, Riming Huang

    Abstract:

    Four new jacaranone analogs, marinoids F–I (1–4), were isolated from the fruits of a Beibu Gulf mangrove Avicennia marina. The structures were elucidated based on analysis of spectroscopic data. Marinoids F and G are shown to be diastereoisomers of chlorocornoside, a new halogen containing marine secondary metabolite. The Antioxidant activity of the isolates was evaluated using a cellular Antioxidant Assay, and 4 showed good Antioxidant activity (EC50 = 26 μM).

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Satyanshu Kumar – One of the best experts on this subject based on the ideXlab platform.

  • effect of extraction methods on yield phytochemical constituents and Antioxidant activity of withania somnifera
    Arabian Journal of Chemistry, 2017
    Co-Authors: Tushar Dhanani, Sonal Shah, N A Gajbhiye, Satyanshu Kumar

    Abstract:

    Abstract Withnaia somnifera (L.) is a wonder herb with multiple medicinal properties. Its root is used in the preparation of many Ayurvedic medicines. Withanolides, steroidal lactones, present in the root are active chemical markers, however, phenolics, and flavonoids have also been reported in the root of this plant. In most of the herbal preparations, water extraction is carried out using the infusion or decoction preparation process. In the present study, extract yield, phytochemical constituents such as total phenol and withanolide content of water and water-alcohol extracts prepared using two most commonly used extraction techniques, also known as “Green Extraction” techniques, ultrasound and microwave assisted solvent extraction were compared with the conventional extraction method. Antioxidant activity of the extracts was also determined using DPPH and ABTS methods of Antioxidant Assay. Extract yield, chemical composition of the extracts (total phenol and withanolide content) and Antioxidant activity of the extracts varied with the extraction process as well as solvent composition.

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R. P. Singh – One of the best experts on this subject based on the ideXlab platform.

  • Antioxidant and antiplatlet aggregation properties of bark extracts of garcinia pedunculata and garcinia cowa
    Journal of Food Science and Technology-mysore, 2014
    Co-Authors: Anushi Sharma, G S Joseph, R. P. Singh

    Abstract:

    The bark extracts of Garcinia pedunculata and Garcinia cowa, which are abundant in the Northeastern regions of India, were screened for their Antioxidant and in vitro antiplatelet aggregating activities. By β-carotene linoleate model for Antioxidant Assay, acetone extract of G. pedunculata and hexane extracts of G. cowa exhibited higher Antioxidant activity (86.47 and 66.94 % respectively, at 25 ppm) than other extracts. Similar pattern was observed for superoxide radical scavenging method for Antioxidant Assay. The ethyl acetate extract of G. pedunculata and hexane extract of G. cowa exhibited higher antiplatelet aggregation capacity towards ADP induced platelet aggregation (IC50 0.16 and 0.43 ug, respectively) than other extracts.

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  • Genesis and development of DPPH method of Antioxidant Assay
    Journal of Food Science and Technology, 2011
    Co-Authors: Sagar B. Kedare, R. P. Singh

    Abstract:

    α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method offers the first approach for evaluating the Antioxidant potential of a compound, an extract or other biological sources. This is the simplest method, wherein the prospective compound or extract is mixed with DPPH solution and absorbance is recorded after a defined period. However, with the advancement and sophistication in instrumental techniques, the method has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them. This article presents a critical review on various developments to the DPPH method.

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