The Experts below are selected from a list of 123 Experts worldwide ranked by ideXlab platform
Claudio Chiesa - One of the best experts on this subject based on the ideXlab platform.
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comparison of nephelometric and hemolytic techniques for determination of Antistreptolysin o antibodies
American Journal of Clinical Pathology, 1995Co-Authors: Lucia Pacifico, G Mancuso, Enrico Properzi, Giampietro Ravagnan, A M Pasquino, Claudio ChiesaAbstract:The sensitivity of a newly devised nephelometric method for determining Antistreptolysin O antibodies was compared with the hemolytic inhibition assay. Three hundred-thirty single serum samples from children with and without evidence of group A streptococcal infection were analyzed by the two techniques. The nephelometric method results correlated well with those of the reference test (concordance : r = 0.88). Furthermore, 134 pairs of acute and convalescent phase sera from patients with culture-proven GAS infection and 50 pairs from children who served as control subjects were examined. The nephelometric assay was more sensitive in detecting significant ASO antibody rises than the hemolytic assay. The automated nephelometric method appears to be a much simpler and sensitive procedure for testing ASO antibodies. (Key words : Antistreptolysin O ; Rate nephelometry) Am J Clin Pathol 1995 ;103 :396-399.
K Toyama - One of the best experts on this subject based on the ideXlab platform.
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high activity of Antistreptolysin o in a case of igm myeloma
The Japanese journal of clinical hematology, 1996Co-Authors: Y Inatomi, Akihiko Gotoh, Yuzuru Kuriyama, S Kuwabara, M Nakano, K ToyamaAbstract:: A 56-year-old man was admitted to our hospital in November, 1991 because of hyperproteinemia and anemia. Total protein showed 12 g/dl and serum immunoglobulins were as follows; IgG 974 mg/dl, IgA 142 mg/dl, IgM 9270 mg/dl. M-component was identified as IgM-kappa with immunoelectrophoresis and serum viscosity indicated 6.9. Although the patient had no history of severe streptococcal infection, his serum showed very high activity of ASLO (6890 IU/ml). Bence Jones protein was detected in the urine and determined to be of kappa-type. Plasma cells occupied 43% of bone marrow nucleated cells and their cytoplasms were stained with FITC-labeled anti-IgM and anti-kappa antibodies. X-ray examination disclosed punched out lesions in the skull. Consequently, the patient was diagnosed as having IgM-myeloma. At first, VCAP regimens were given after plasmapheresis, but had no effect. Therefore, the patient was treated with CHOP protocol and the serum IgM decreased in amount and the proportion of bone marrow myeloma cells got down to 17%. ASLO titer also decreased in parallel with IgM. These findings suggest that IgM in this case had ASLO activity.
Lucia Pacifico - One of the best experts on this subject based on the ideXlab platform.
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comparison of nephelometric and hemolytic techniques for determination of Antistreptolysin o antibodies
American Journal of Clinical Pathology, 1995Co-Authors: Lucia Pacifico, G Mancuso, Enrico Properzi, Giampietro Ravagnan, A M Pasquino, Claudio ChiesaAbstract:The sensitivity of a newly devised nephelometric method for determining Antistreptolysin O antibodies was compared with the hemolytic inhibition assay. Three hundred-thirty single serum samples from children with and without evidence of group A streptococcal infection were analyzed by the two techniques. The nephelometric method results correlated well with those of the reference test (concordance : r = 0.88). Furthermore, 134 pairs of acute and convalescent phase sera from patients with culture-proven GAS infection and 50 pairs from children who served as control subjects were examined. The nephelometric assay was more sensitive in detecting significant ASO antibody rises than the hemolytic assay. The automated nephelometric method appears to be a much simpler and sensitive procedure for testing ASO antibodies. (Key words : Antistreptolysin O ; Rate nephelometry) Am J Clin Pathol 1995 ;103 :396-399.
Athimalaipet V Ramanan - One of the best experts on this subject based on the ideXlab platform.
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how to use Antistreptolysin o titre
Archives of Disease in Childhood, 2014Co-Authors: Athimalaipet V RamananAbstract:Group A streptococcus (GAS) is the cause of a wide range of acute suppurative and, following a latent period, non-suppurative diseases such as rheumatic fever and poststreptococcal glomerulonephritis. Diagnosis of the latter group requires evidence of preceding GAS infection. The bacteria produce a range of extracellular antigens, including streptolysin O, which induce an antibody response in the host. A rise in Antistreptolysin O titre (ASOT) is indicative of preceding GAS infection. In clinical practice, often only a single ASOT measurement is available and its timing in relation to a possible GAS infection is unknown. Interpretation of the result in this context is liable to misdiagnosis. In order to optimise diagnosis of preceding GAS infection, at least two sequential ASOT measurements, together with simultaneous assay for anti-DNase B, a second antistreptococcal antibody, is recommended.
Y Inatomi - One of the best experts on this subject based on the ideXlab platform.
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high activity of Antistreptolysin o in a case of igm myeloma
The Japanese journal of clinical hematology, 1996Co-Authors: Y Inatomi, Akihiko Gotoh, Yuzuru Kuriyama, S Kuwabara, M Nakano, K ToyamaAbstract:: A 56-year-old man was admitted to our hospital in November, 1991 because of hyperproteinemia and anemia. Total protein showed 12 g/dl and serum immunoglobulins were as follows; IgG 974 mg/dl, IgA 142 mg/dl, IgM 9270 mg/dl. M-component was identified as IgM-kappa with immunoelectrophoresis and serum viscosity indicated 6.9. Although the patient had no history of severe streptococcal infection, his serum showed very high activity of ASLO (6890 IU/ml). Bence Jones protein was detected in the urine and determined to be of kappa-type. Plasma cells occupied 43% of bone marrow nucleated cells and their cytoplasms were stained with FITC-labeled anti-IgM and anti-kappa antibodies. X-ray examination disclosed punched out lesions in the skull. Consequently, the patient was diagnosed as having IgM-myeloma. At first, VCAP regimens were given after plasmapheresis, but had no effect. Therefore, the patient was treated with CHOP protocol and the serum IgM decreased in amount and the proportion of bone marrow myeloma cells got down to 17%. ASLO titer also decreased in parallel with IgM. These findings suggest that IgM in this case had ASLO activity.
