The Experts below are selected from a list of 68316 Experts worldwide ranked by ideXlab platform

Jose A Feijo - One of the best experts on this subject based on the ideXlab platform.

  • signaling with ions the keystone for Apical Cell growth and morphogenesis in pollen tubes
    Plant Physiology, 2017
    Co-Authors: Erwan Michard, Alexander A Simon, Barbara Tavares, Michael M Wudick, Jose A Feijo
    Abstract:

    Ion homeostasis and signaling are crucial to regulate pollen tube growth and morphogenesis and affect upstream membrane transporters and downstream targets.

  • transcriptional profiling of arabidopsis root hairs and pollen defines an Apical Cell growth signature
    BMC Plant Biology, 2014
    Co-Authors: Jorg D Becker, Seiji Takeda, Filipe Borges, Liam Dolan, Jose A Feijo
    Abstract:

    Background Current views on the control of Cell development are anchored on the notion that phenotypes are defined by networks of transcriptional activity. The large amounts of information brought about by transcriptomics should allow the definition of these networks through the analysis of Cell-specific transcriptional signatures. Here we test this principle by applying an analogue to comparative anatomy at the Cellular level, searching for conserved transcriptional signatures, or conserved small gene-regulatory networks (GRNs) on root hairs (RH) and pollen tubes (PT), two filamentous Apical growing Cells that are a striking example of conservation of structure and function in plants.

  • transcriptional profiling of arabidopsis root hairs and pollen defines an Apical Cell growth signature
    BMC Plant Biology, 2014
    Co-Authors: Jorg D Becker, Seiji Takeda, Filipe Borges, Liam Dolan, Jose A Feijo
    Abstract:

    Current views on the control of Cell development are anchored on the notion that phenotypes are defined by networks of transcriptional activity. The large amounts of information brought about by transcriptomics should allow the definition of these networks through the analysis of Cell-specific transcriptional signatures. Here we test this principle by applying an analogue to comparative anatomy at the Cellular level, searching for conserved transcriptional signatures, or conserved small gene-regulatory networks (GRNs) on root hairs (RH) and pollen tubes (PT), two filamentous Apical growing Cells that are a striking example of conservation of structure and function in plants. We developed a new method for isolation of growing and mature root hair Cells, analysed their transcriptome by microarray analysis, and further compared it with pollen and other single Cell transcriptomics data. Principal component analysis shows a statistical relation between the datasets of RHs and PTs which is suggestive of a common transcriptional profile pattern for the Apical growing Cells in a plant, with overlapping profiles and clear similarities at the level of small GTPases, vesicle-mediated transport and various specific metabolic responses. Furthermore, cis-regulatory element analysis of co-regulated genes between RHs and PTs revealed conserved binding sequences that are likely required for the expression of genes comprising the Apical signature. This included a significant occurrence of motifs associated to a defined transcriptional response upon anaerobiosis. Our results suggest that maintaining Apical growth mechanisms synchronized with energy yielding might require a combinatorial network of transcriptional regulation. We propose that this study should constitute the foundation for further genetic and physiological dissection of the mechanisms underlying Apical growth of plant Cells.

Siobhan A Braybrook - One of the best experts on this subject based on the ideXlab platform.

  • towards an understanding of spiral patterning in the sargassum muticum shoot apex
    Scientific Reports, 2017
    Co-Authors: Marina Linardic, Siobhan A Braybrook
    Abstract:

    In plants and parenchymatous brown algae the body arises through the activity of an Apical meristem (a niche of Cells or a single Cell). The meristem produces lateral organs in specific patterns, referred to as phyllotaxis. In plants, two different control mechanisms have been proposed: one is position-dependent and relies on morphogen accumulation at future organ sites; the other is a lineage-based system which links phyllotaxis to the Apical Cell division pattern. Here we examine the Apical patterning of the brown alga, Sargassum muticum, which exhibits spiral phyllotaxis (137.5° angle) and an unlinked Apical Cell division pattern. The Sargassum apex presents characteristics of a self-organising system, similar to plant meristems. In contrast to complex plant meristems, we were unable to correlate the plant morphogen auxin with bud positioning in Sargassum, nor could we predict Cell wall softening at new bud sites. Our data suggests that in Sargassum muticum there is no connection between phyllotaxis and the Apical Cell division pattern indicating a position-dependent patterning mechanism may be in place. The underlying mechanisms behind the phyllotactic patterning appear to be distinct from those seen in plants.

  • towards an understanding of spiral patterning in the sargassum muticum shoot apex
    bioRxiv, 2017
    Co-Authors: Marina Linardic, Siobhan A Braybrook
    Abstract:

    In plants and parenchymatous brown algae the body arises through the activity of an Apical meristem (a niche of Cells or a single Cell). The meristem produces lateral organs in specific patterns, referred to as phyllotaxis. In plants, two different control mechanisms have been proposed; one is position-dependent and relies on morphogen accumulation at future organ sites whereas the other is a lineage-based system which links phyllotaxis to the Apical Cell division pattern. Here we examine the Apical patterning of the brown alga, Sargassum muticum, which exhibits spiral phyllotaxis (137.5 degree angle) and an unlinked Apical Cell division pattern. The Sargassum apex presents characteristics of a self-organising system, similar to plant meristems. We were unable to correlate the plant morphogen auxin with bud positioning in Sargassum, nor could we predict Cell wall softening at new bud sites. Our data suggests that in Sargassum muticum there is no connection between phyllotaxis and the Apical Cell division pattern indicating a position-dependent patterning mechanism may be in place. The underlying mechanisms behind the phyllotactic patterning appear to be distinct from those seen in plants.

Seiji Takeda - One of the best experts on this subject based on the ideXlab platform.

  • transcriptional profiling of arabidopsis root hairs and pollen defines an Apical Cell growth signature
    BMC Plant Biology, 2014
    Co-Authors: Jorg D Becker, Seiji Takeda, Filipe Borges, Liam Dolan, Jose A Feijo
    Abstract:

    Background Current views on the control of Cell development are anchored on the notion that phenotypes are defined by networks of transcriptional activity. The large amounts of information brought about by transcriptomics should allow the definition of these networks through the analysis of Cell-specific transcriptional signatures. Here we test this principle by applying an analogue to comparative anatomy at the Cellular level, searching for conserved transcriptional signatures, or conserved small gene-regulatory networks (GRNs) on root hairs (RH) and pollen tubes (PT), two filamentous Apical growing Cells that are a striking example of conservation of structure and function in plants.

  • transcriptional profiling of arabidopsis root hairs and pollen defines an Apical Cell growth signature
    BMC Plant Biology, 2014
    Co-Authors: Jorg D Becker, Seiji Takeda, Filipe Borges, Liam Dolan, Jose A Feijo
    Abstract:

    Current views on the control of Cell development are anchored on the notion that phenotypes are defined by networks of transcriptional activity. The large amounts of information brought about by transcriptomics should allow the definition of these networks through the analysis of Cell-specific transcriptional signatures. Here we test this principle by applying an analogue to comparative anatomy at the Cellular level, searching for conserved transcriptional signatures, or conserved small gene-regulatory networks (GRNs) on root hairs (RH) and pollen tubes (PT), two filamentous Apical growing Cells that are a striking example of conservation of structure and function in plants. We developed a new method for isolation of growing and mature root hair Cells, analysed their transcriptome by microarray analysis, and further compared it with pollen and other single Cell transcriptomics data. Principal component analysis shows a statistical relation between the datasets of RHs and PTs which is suggestive of a common transcriptional profile pattern for the Apical growing Cells in a plant, with overlapping profiles and clear similarities at the level of small GTPases, vesicle-mediated transport and various specific metabolic responses. Furthermore, cis-regulatory element analysis of co-regulated genes between RHs and PTs revealed conserved binding sequences that are likely required for the expression of genes comprising the Apical signature. This included a significant occurrence of motifs associated to a defined transcriptional response upon anaerobiosis. Our results suggest that maintaining Apical growth mechanisms synchronized with energy yielding might require a combinatorial network of transcriptional regulation. We propose that this study should constitute the foundation for further genetic and physiological dissection of the mechanisms underlying Apical growth of plant Cells.

Liam Dolan - One of the best experts on this subject based on the ideXlab platform.

  • transcriptional profiling of arabidopsis root hairs and pollen defines an Apical Cell growth signature
    BMC Plant Biology, 2014
    Co-Authors: Jorg D Becker, Seiji Takeda, Filipe Borges, Liam Dolan, Jose A Feijo
    Abstract:

    Background Current views on the control of Cell development are anchored on the notion that phenotypes are defined by networks of transcriptional activity. The large amounts of information brought about by transcriptomics should allow the definition of these networks through the analysis of Cell-specific transcriptional signatures. Here we test this principle by applying an analogue to comparative anatomy at the Cellular level, searching for conserved transcriptional signatures, or conserved small gene-regulatory networks (GRNs) on root hairs (RH) and pollen tubes (PT), two filamentous Apical growing Cells that are a striking example of conservation of structure and function in plants.

  • transcriptional profiling of arabidopsis root hairs and pollen defines an Apical Cell growth signature
    BMC Plant Biology, 2014
    Co-Authors: Jorg D Becker, Seiji Takeda, Filipe Borges, Liam Dolan, Jose A Feijo
    Abstract:

    Current views on the control of Cell development are anchored on the notion that phenotypes are defined by networks of transcriptional activity. The large amounts of information brought about by transcriptomics should allow the definition of these networks through the analysis of Cell-specific transcriptional signatures. Here we test this principle by applying an analogue to comparative anatomy at the Cellular level, searching for conserved transcriptional signatures, or conserved small gene-regulatory networks (GRNs) on root hairs (RH) and pollen tubes (PT), two filamentous Apical growing Cells that are a striking example of conservation of structure and function in plants. We developed a new method for isolation of growing and mature root hair Cells, analysed their transcriptome by microarray analysis, and further compared it with pollen and other single Cell transcriptomics data. Principal component analysis shows a statistical relation between the datasets of RHs and PTs which is suggestive of a common transcriptional profile pattern for the Apical growing Cells in a plant, with overlapping profiles and clear similarities at the level of small GTPases, vesicle-mediated transport and various specific metabolic responses. Furthermore, cis-regulatory element analysis of co-regulated genes between RHs and PTs revealed conserved binding sequences that are likely required for the expression of genes comprising the Apical signature. This included a significant occurrence of motifs associated to a defined transcriptional response upon anaerobiosis. Our results suggest that maintaining Apical growth mechanisms synchronized with energy yielding might require a combinatorial network of transcriptional regulation. We propose that this study should constitute the foundation for further genetic and physiological dissection of the mechanisms underlying Apical growth of plant Cells.

Filipe Borges - One of the best experts on this subject based on the ideXlab platform.

  • transcriptional profiling of arabidopsis root hairs and pollen defines an Apical Cell growth signature
    BMC Plant Biology, 2014
    Co-Authors: Jorg D Becker, Seiji Takeda, Filipe Borges, Liam Dolan, Jose A Feijo
    Abstract:

    Background Current views on the control of Cell development are anchored on the notion that phenotypes are defined by networks of transcriptional activity. The large amounts of information brought about by transcriptomics should allow the definition of these networks through the analysis of Cell-specific transcriptional signatures. Here we test this principle by applying an analogue to comparative anatomy at the Cellular level, searching for conserved transcriptional signatures, or conserved small gene-regulatory networks (GRNs) on root hairs (RH) and pollen tubes (PT), two filamentous Apical growing Cells that are a striking example of conservation of structure and function in plants.

  • transcriptional profiling of arabidopsis root hairs and pollen defines an Apical Cell growth signature
    BMC Plant Biology, 2014
    Co-Authors: Jorg D Becker, Seiji Takeda, Filipe Borges, Liam Dolan, Jose A Feijo
    Abstract:

    Current views on the control of Cell development are anchored on the notion that phenotypes are defined by networks of transcriptional activity. The large amounts of information brought about by transcriptomics should allow the definition of these networks through the analysis of Cell-specific transcriptional signatures. Here we test this principle by applying an analogue to comparative anatomy at the Cellular level, searching for conserved transcriptional signatures, or conserved small gene-regulatory networks (GRNs) on root hairs (RH) and pollen tubes (PT), two filamentous Apical growing Cells that are a striking example of conservation of structure and function in plants. We developed a new method for isolation of growing and mature root hair Cells, analysed their transcriptome by microarray analysis, and further compared it with pollen and other single Cell transcriptomics data. Principal component analysis shows a statistical relation between the datasets of RHs and PTs which is suggestive of a common transcriptional profile pattern for the Apical growing Cells in a plant, with overlapping profiles and clear similarities at the level of small GTPases, vesicle-mediated transport and various specific metabolic responses. Furthermore, cis-regulatory element analysis of co-regulated genes between RHs and PTs revealed conserved binding sequences that are likely required for the expression of genes comprising the Apical signature. This included a significant occurrence of motifs associated to a defined transcriptional response upon anaerobiosis. Our results suggest that maintaining Apical growth mechanisms synchronized with energy yielding might require a combinatorial network of transcriptional regulation. We propose that this study should constitute the foundation for further genetic and physiological dissection of the mechanisms underlying Apical growth of plant Cells.